{"id":37400,"date":"2023-01-09T11:39:30","date_gmt":"2023-01-09T03:39:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37400"},"modified":"2023-01-16T11:48:27","modified_gmt":"2023-01-16T03:48:27","slug":"atf4-lentiviral-vector-human-cmv-plenti-giii-cmv-gfp-2a-puro%e8%b2%a8%e8%99%9f126100610395","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37400","title":{"rendered":"ATF4 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)\u8ca8\u865f126100610395"},"content":{"rendered":"

ATF4 Lentiviral Vector \u6162\u75c5\u6bd2\u8f09\u9ad4(pLenti-GIII-CMV-GFP-2A-Puro) |\u00a0\u8ca8\u865f126100610395<\/a><\/span><\/strong><\/p>\n

\"pORF\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name<\/th>\nATF4 Lentiviral Vector (Human) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)<\/td>\n<\/tr>\n
Accession Number<\/th>\nBC016855<\/td>\n<\/tr>\n
Description<\/th>\nThe GOI expression will be driven by a CMV promoter, with a GFP reporter. Inserts are flanked by EcoRV sites.<\/td>\n<\/tr>\n
Cat.No<\/th>\n126100610395<\/td>\n<\/tr>\n
Gene Name<\/th>\nATF4<\/td>\n<\/tr>\n
Full Gene Name<\/th>\nactivating transcription factor 4 (tax-responsive enhancer element B67)<\/td>\n<\/tr>\n
Aliases<\/th>\nTAXREB67, CREB-2<\/td>\n<\/tr>\n
List of species<\/th>\nHuman<\/td>\n<\/tr>\n
Units<\/th>\n1.0 \u00b5g DNA<\/td>\n<\/tr>\n
Vector<\/th>\npLenti-GIII-CMV-GFP-2A-Puro<\/a><\/td>\n<\/tr>\n
Applications<\/th>\n\n
    \n
  • Direct non-viral plasmid transfection for immediate expression<\/li>\n
  • Package into lentiviral particles for high efficiency transduction and stably integrated expression<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
System<\/th>\nLentiviral Vector<\/td>\n<\/tr>\n
Insert Size<\/th>\n1056bp<\/td>\n<\/tr>\n
List of Markers<\/th>\nBacterial: Kanamycin. Mammalian: Puromycin<\/td>\n<\/tr>\n
Promoters<\/th>\nCMV<\/td>\n<\/tr>\n
Tag<\/th>\nGFP<\/td>\n<\/tr>\n
Appearance<\/th>\nClear Liquid<\/td>\n<\/tr>\n
Vector Size<\/th>\n9000bp<\/td>\n<\/tr>\n
Format<\/th>\nLentiviral Plasmid<\/td>\n<\/tr>\n
Storage Buffer<\/th>\n10mM Tris-HCI, 1mM EDTA, pH8.0<\/td>\n<\/tr>\n
Storage<\/th>\n1 year when stored at -20\u00b0C or lower in a non-frost free freezer.<\/td>\n<\/tr>\n
Reconstitution<\/th>\n5′-NheI and 3′-BamHI<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n
Vector Name<\/b><\/td>\npLenti-GIII-CMV-GFP-2A-Puro<\/td>\n<\/tr>\n
VectorType<\/b><\/td>\nLentiviral Plasmid<\/td>\n<\/tr>\n
Antibiotic Information<\/b><\/td>\nBacterial: Kanamycin. Mammalian: Puromycin.<\/td>\n<\/tr>\n
Sequencing Primers<\/b><\/td>\nCMV sequencing primer
\n5`—CGC AAA TGG GCG GTA GGC GTG—3`<\/td>\n<\/tr>\n
Additional Information<\/b><\/td>\nThe gene expression cassette is driven by a CMV promoter, with a GFP reporter under the control of a separate promoter<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/a><\/p>\n

\uff08ABM\uff09\u6210\u7acb\u65bc2004\u5e74\uff0c\u5728\u50ac\u5316\u751f\u547d\u79d1\u5b78\u548c\u85e5\u7269\u958b\u767c\u7684\u79d1\u5b78\u767c\u73fe\u65b9\u9762\u5df2\u6709\u5341\u591a\u5e74\u7684\u6b77\u53f2\u3002\u7e3d\u90e8\u4f4d\u65bc\u52a0\u62ff\u5927\u6eab\u54e5\u83ef\uff0cabm \u5728\u7814\u767c\u548c\u5546\u696d\u5316\u65b0\u7a4e\u7814\u7a76\u5de5\u5177\u65b9\u9762\u7684\u654f\u6377\u6027\u4ee5\u53caabm\u7368\u7279\u670d\u52d9\u7684\u5b8c\u6574\u7d44\u5408\u4f7fabm\u6210\u70ba\u5168\u7403\u5be6\u9a57\u5ba4\u548c\u7814\u7a76\u6a5f\u69cb\u7684\u5bf6\u8cb4\u5408\u4f5c\u5925\u4f34\u3002\u5728\u4e00\u7fa4\u5145\u6eff\u6fc0\u60c5\u548c\u5929\u624d\u7684\u79d1\u5b78\u5bb6\u7684\u63a8\u52d5\u4e0b\uff0cabm\u81f4\u529b\u65bc\u70ba\u79d1\u5b78\u754c\u63d0\u4f9b\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u85e5\u7269\u958b\u767c\u65b9\u9762\u7684\u6700\u65b0\u5275\u65b0\u6210\u679c\u3002\"\"<\/p>\n

abm \u516c\u53f8\u4e00\u76f4\u88ab\u516c\u8a8d\u70baB.C.\u767c\u5c55\u6700\u5feb\u768425\u5bb6\u516c\u53f8\u4e4b\u4e00\uff0cabm\u516c\u53f8\u81ea\u6210\u7acb\u4ee5\u4f86\u5e74\u5747\u4fdd\u630130\uff05\u7684\u5e74\u5747\u589e\u9577\u7387\uff0c\u4e26\u4fdd\u6301\u5e74\u5747\u6307\u6578\u589e\u9577\u7387\u548c\u53ef\u6301\u7e8c\u589e\u9577\u7387\u3002\u4eca\u5929\uff0cabm\u5f88\u81ea\u8c6a\u80fd\u5920\u6210\u70ba\u8a72\u5730\u5340\u6700\u5927\u7684\u751f\u7269\u6280\u8853\u516c\u53f8\u4e4b\u4e00\uff0c\u70baB.C.\u4e2d\u5145\u6eff\u6d3b\u529b\u548c\u5feb\u7bc0\u594f\u7684\u751f\u7269\u6280\u8853\u7522\u696d\u4f5c\u51fa\u8ca2\u737b\u3002abm \u8207\u8457\u540d\u5927\u5b78\u548c\u7814\u767c\u6a5f\u69cb\u4ee5\u53caabm \u5168\u7403\u5206\u92b7\u7db2\u7d61\u7684\u9577\u671f\u570b\u969b\u5408\u4f5c\u5925\u4f34\u95dc\u4fc2\u4f7fabm \u80fd\u5920\u5728\u5168\u7403\u7bc4\u570d\u5167\u63a8\u52d5\u767c\u73fe\u3002<\/p>\n

abm \u7684\u4f7f\u547d\u662f\u5e6b\u52a9\u79d1\u5b78\u5bb6\u901a\u904e\u6539\u8b8a\u904a\u6232\u898f\u5247\u7684\u7522\u54c1\u548c\u670d\u52d9\u8df3\u8e8d\u5230\u4ed6\u5011\u7684\u4e0b\u4e00\u500b\u7814\u7a76\u91cc\u7a0b\u7891\uff0c\u5f9e\u800c\u7c21\u5316\u89e3\u6c7a\u65b9\u6848\u4e26\u964d\u4f4e\u6210\u672c\u3002 abm \u6db5\u84cb\u6240\u6709\u7522\u54c1\u7684\u9023\u7e8c\u7522\u54c1\u6db5\u84cbCRISPR\u57fa\u56e0\u7de8\u8f2f\u5de5\u5177\u548c\u75c5\u6bd2\u8f09\u9ad4\/\u75c5\u6bd2\uff0c\u4e16\u754c\u4e0a\u6700\u5927\u7684\u7368\u7279\u7d30\u80de\u4fc2\u4ee5\u53ca\u6700\u5148\u9032\u7684PCR\u548c\u4e0b\u4e00\u4ee3\u6e2c\u5e8f\u6280\u8853\u548c\u670d\u52d9\u3002 abm \u63d0\u4f9b\u9ad8\u5ea6\u91cf\u8eab\u5b9a\u5236\u7684\u670d\u52d9\u7684\u80fd\u529b\u4f7f\u7814\u7a76\u4eba\u54e1\u80fd\u5920\u89e3\u6c7a\u4ed6\u5011\u6700\u7dca\u8feb\u7684\u6311\u6230\uff0c\u4e26\u4ee5\u65b0\u7a4e\u5275\u65b0\u7684\u65b9\u5f0f\u5c07\u7814\u7a76\u4e2d\u7684\u9ede\u9ede\u9023\u63a5\u8d77\u4f86\u3002abm \u7684\u9858\u666f\u662f\u6210\u70ba\u9ad8\u8cea\u91cf\u548c\u9ad8\u6027\u50f9\u6bd4\u7814\u7a76\u8a66\u5291\u7684\u4e00\u7ad9\u5f0f\u5546\u5e97\uff0c\u6eff\u8db3\u85e5\u7269\u958b\u767c\u548c\u751f\u7269\u88fd\u85e5\u5e02\u5834\u7684\u8feb\u5207\u9700\u6c42\u3002 \u4f5c\u70ba\u88fd\u9020\u5546\u548c\u670d\u52d9\u63d0\u4f9b\u5546\uff0cabm\u5e0c\u671b\u7c21\u5316\u65e5\u5e38\u79d1\u5b78\u4ee5\u52a0\u901f\u60a8\u7684\u91cd\u5927\u767c\u73fe\u3002<\/p>\n

HOT!!<\/strong><\/span>\u00a0\u9ef4\u6f3f\u83ccPCR\u5075\u6e2c<\/span><\/a>\u00a0|\u00a0DAPI\u67d3\u6838<\/a>\u00a0|\u00a0\u7d30\u80de\u5167\u9ef4\u6f3f\u83cc\u53bb\u9664\u5291<\/a>\u00a0|\u00a0\u6c34\u6d74\u69fd\u6291\u83cc\u5291<\/a>\u00a0|\u00a0\u5674\u9727\u5f0f\u53bb\u9ef4\u5291 \u7b49\u7cfb\u5217\u5546\u54c1<\/a><\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

ATF4 Lentiviral Vector \u6162\u75c5\u6bd2\u8f09\u9ad4(pLenti-GIII-CMV-GFP-2A-Pur […]<\/p>\n","protected":false},"author":3,"featured_media":37401,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[133],"tags":[5139],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37400"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37400"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37400\/revisions"}],"predecessor-version":[{"id":37402,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37400\/revisions\/37402"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/37401"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37400"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37400"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37400"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}