{"id":37120,"date":"2022-12-22T18:34:33","date_gmt":"2022-12-22T10:34:33","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37120"},"modified":"2022-12-27T10:53:21","modified_gmt":"2022-12-27T02:53:21","slug":"chekine-%e7%b2%92%e7%b7%9a%e9%ab%94%e8%a4%87%e5%90%88%e7%89%a9%e2%85%a4%e6%b4%bb%e6%80%a7%e6%b8%ac%e5%ae%9a%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-micro-mitochondrial-complex%e2%85%a4","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37120","title":{"rendered":"CheKine\u2122 \u7c92\u7dda\u9ad4\u8907\u5408\u7269\u2164\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Mitochondrial complex\u2164 Activity Assay Kit\u8ca8\u865fKTB1890"},"content":{"rendered":"

CheKine\u2122 \u7c92\u7dda\u9ad4\u8907\u5408\u7269\u2164<\/strong>\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Mitochondrial complex\u2164 Activity Assay Kit<\/strong>\u8ca8\u865f<\/strong>KTB1890<\/a><\/span><\/p>\n

CheKine\u2122 Micro Mitochondrial complex \u2164 Activity Assay Kit<\/span><\/p>\n

\u7dda\u7c92\u9ad4\u547c\u5438\u93c8\u8907\u5408\u9ad4V(Mitochondrial respiratory chain complex V)\uff0c\u53c8\u7a31F1F0-ATP\u5408\u9176(F1F0-ATP synthase)\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u7684\u7c92\u7dda\u9ad4\u4e2d\u3002\u8907\u5408\u7269 V \u7531\u5169\u500b\u4e9e\u57fa F1 \u548c F0 \u7d44\u6210\u3002\u5b83\u53ef\u4ee5\u5229\u7528\u547c\u5438\u93c8\u7522\u751f\u7684\u8cea\u5b50\u7684\u96fb\u5316\u5b78\u68af\u5ea6(electrochemical gradient)\u50ac\u5316ATP\u7684\u5408\u6210\uff0c\u4e5f\u53ef\u4ee5\u5728\u53ef\u9006(reversible process)\u904e\u7a0b\u4e2d\u6c34\u89e3ATP\u3002\u6b64\u5916\uff0c\u8907\u5408\u7269V(complex V)\u9084\u5b58\u5728\u65bc\u8449\u7da0\u9ad4(chloroplasts)\u3001\u7570\u990a\u7d30\u83cc(heterotrophic bacteria)\u548c\u5149\u5408\u7d30\u83cc(photosynthetic bacteria)\u4e2d\u3002 Complex V\u662f\u7c92\u7dda\u9ad4\u6c27\u5316\u78f7\u9178\u5316(mitochondrial oxidative phosphorylation)\u548c\u8449\u7da0\u9ad4(chloroplast)\u5149\u5408\u78f7\u9178\u5316\u5408\u6210ATP\u7684\u95dc\u9375\u9176\u3002<\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong><\/span><\/p>\n

Abbkine CheKine\u2122 Micro Mitochondrial complex \u2164\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(Abbkine CheKine\u2122 Micro Mitochondrial complex \u2164 Activity Detection Kit, KTB1890)\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u9748\u654f\u6e96\u78ba\u3002\u8907\u5408\u7269\u2164\u6c34\u89e3ATP\u7522\u751fADP\u548cPi\u3002\u8907\u5408\u7269\u2164\u7684\u6d3b\u6027\u901a\u904e\u6e2c\u5b9aPi\u589e\u52a0\u7387\u4f86\u78ba\u5b9a\u3002<\/p>\n

\u00a0\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/strong><\/p>\n

\"HOT\"\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u9ad4 I \uff08Mitochondrial respiratory chain complex I , EC 1.6.5. 3\uff09\uff0c\u53c8\u7a31NADH-CoQ\u9084\u539f\u9176(NADH-CoQ reductase)\u6216NADH\u812b\u6c2b\u9176(NADH dehydrogenase)\uff0c\u662f\u7c92\u7dda\u9ad4\u5167\u819c\u4e2d\u6700\u5927\u7684\u86cb\u767d\u8cea\u8907\u5408\u9ad4\u3002 Complex I\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u7684\u7c92\u7dda\u9ad4\u4e2d\u3002\u9019\u7a2e\u9176\u50ac\u5316\u4e00\u5c0d\u96fb\u5b50\u5f9eNADH\u8f49\u79fb\u5230CoQ\uff0c\u540c\u6642\u53ef\u4ee5\u9084\u539fO2\u7522\u751fO2-\uff0c\u662f\u547c\u5438\u96fb\u5b50\u50b3\u905e\u93c8\u7522\u751fO2-\u7684\u4e3b\u8981\u90e8\u5206\u3002 Complex I\u7684\u6d3b\u6027\u4e0d\u50c5\u53ef\u4ee5\u53cd\u6620\u8cac\u4efb\u96fb\u5b50\u50b3\u905e\u93c8\uff08ETC\uff09\u7684\u72c0\u614b\uff0c\u9084\u53ef\u4ee5\u53cd\u6620\u6d3b\u6027\u6c27\uff08ROS\uff09\u7684\u7522\u751f\u3002CheKine\u2122 \u7c92\u7dda\u9ad4\u8907\u5408\u7269 I \u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Micro Mitochondrial complex I Activity Assay Kit\u8ca8\u865fKTB1850<\/a><\/span>\u5c08\u70ba\u6aa2\u6e2c\u8907\u5408\u7269I(Complex I)\u6d3b\u6027\u800c\u958b\u767c\u3002\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u9748\u654f\u6e96\u78ba\u3002\u8907\u5408\u7269 I (Complex I)\u53ef\u4ee5\u50ac\u5316 NADH \u812b\u6c2b\u70ba NAD+\u3002\u53ef\u4ee5\u5728 340 nm \u8655\u6e2c\u5b9a NADH \u7684\u6c27\u5316\u901f\u7387\uff0c\u4ee5\u8a08\u7b97\u8907\u5408\u7269 I \u7684\u6d3b\u6027\u3002<\/p>\n

\"HOT\"\u7c92\u7dda\u9ad4\u8907\u5408\u7269\u2161(Mitochondrial complex\u2161)\u53c8\u540d\u7425\u73c0\u9178\u8f14\u9176Q\u9084\u539f\u9176(succinic acid-coenzyme Q reductase)\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u3002 Complex\u2161\u53ef\u50ac\u5316\u7425\u73c0\u9178(succinic acid)\u6c27\u5316\u751f\u6210\u5bcc\u99ac\u9178(fumaric acid)\uff0c\u800c\u8f14\u56e0\u5b50 FAD (cofactor FAD\u9084\u539f)\u70ba FADH2\uff0c\u9032\u4e00\u6b65\u9084\u539f\u6c27\u5316\u578b\u8f14\u9176 Q \u7522\u751f\u9084\u539f\u578b\u8f14\u9176 Q(reduced coenzyme Q)\uff0c\u9019\u662f\u4e00\u500b\u5206\u652f(branch)\u547c\u5438\u96fb\u5b50\u50b3\u905e\u93c8(respiratory electron transport chain)CheKine\u2122 Micro Mitochondrial Complex\u2161\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(CheKine\u2122 Micro Mitochondrial Complex\u2161 Activity Assay Kit, KTB1860)<\/a><\/span>\u63d0\u4f9b\u4e86\u4e00\u7a2e\u65b9\u4fbf\u7684\u7c92\u7dda\u9ad4\u8907\u5408\u7269\u2161\u6d3b\u6027(Mitochondrial complex\u2161 Activity)\u6aa2\u6e2c\u5de5\u5177\u3002\u539f\u7406\u662f\u9084\u539f\u578b\u8f14\u9176Q(reduced coenzyme Q)\uff0c\u5b83\u662f\u50ac\u5316\u7522\u7269\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269\u2161(mitochondrial respiratory chain complex\u2161)\u53ef\u9032\u4e00\u6b65\u9084\u539f\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0(absorption peak)\u76842,6-\u4e8c\u6c2f\u5432\u54da(2,6-dichloroindoxyl)( 605nm)\u3002\u56e0\u6b64\uff0ccomplex\u2161\u7684\u6d3b\u6027\u53ef\u4ee5\u6839\u64da2,6-\u4e8c\u6c2f\u5432\u54da(2,6-dichloroindoxyl)( 605nm)\u7684\u6e1b\u5c11\u7387\u4f86\u8a08\u7b97\u3002<\/p>\n

\"HOT\"\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269III\uff08Mitochondrial respiratory chain complex III , EC 1.10.2.2\uff09\uff0c\u53c8\u7a31\u8f14\u9176Q-\u7d30\u80de\u8272\u7d20C\u9084\u539f\u9176(CoQ-cytochrome C reductase)\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u7684\u7c92\u7dda\u9ad4\u4e2d\u3002\u5b83\u5728\u7c92\u7dda\u9ad4\u547c\u5438\u96fb\u5b50\u50b3\u905e\u93c8(mitochondrial respiratory electron transport chain)\u7684\u4e3b\u8981\u548c\u5206\u652f\u901a\u8def\u4e2d\u8d77\u8457\u95dc\u9375\u4f5c\u7528\u3002 Complex III\u53ef\u4ee5\u5c07\u9084\u539f\u578bCoQ(reduced CoQ)\u4e2d\u7684\u6c2b(hydrogen)\u8f49\u79fb\u5230\u7d30\u80de\u8272\u7d20C(cytochrome C)\u4e0a\uff0c\u7522\u751f\u9084\u539f\u578b\u7d30\u80de\u8272\u7d20C(reduced cytochrome C)\u3002Abbkine CheKine\u2122 Micro Mitochondrial Complex \u2162\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(Abbkine CheKine\u2122 Micro Mitochondrial Complex \u2162 Activity Detection Kit, KTB1870)<\/a><\/span>\u5c08\u70ba\u6aa2\u6e2c\u8907\u5408\u7269I\u6d3b\u6027(complex I activity)\u800c\u958b\u767c\u3002\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u9748\u654f\u6e96\u78ba\u3002\u8907\u5408\u7269 III(Complex \u2162 ) \u53ef\u4ee5\u5c07\u6c2b\u5f9e\u9084\u539f\u7684 CoQ \u8f49\u79fb\u5230\u7d30\u80de\u8272\u7d20 C \u4ee5\u7522\u751f\u9084\u539f\u7684\u7d30\u80de\u8272\u7d20 C(cytochrome C)\u3002\u8207\u6c27\u5316\u7684\u7d30\u80de\u8272\u7d20 C(oxidized cytochrome C )\u4e0d\u540c\uff0c\u9084\u539f\u7684\u7d30\u80de\u8272\u7d20 C(reduced cytochrome C) \u5728 550 nm \u8655\u5177\u6709\u7279\u5fb5\u5149\u5438\u6536(light absorption550nm)\u3002\u56e0\u6b64\uff0c\u5149\u5438\u6536\u7387(light absorption rate)\u7684\u589e\u52a0\u53ef\u4ee5\u53cd\u6620Complex III\u9176\u7684\u6d3b\u6027\uff0cAbbkine CheKine\u2122 Micro Mitochondrial complex III Activity Assay Kit\u53ef\u4ee5\u6aa2\u6e2c\u52d5\u690d\u7269\u7d44\u7e54(animal and plant tissues)\u548c\u7d30\u80de\u6a23\u54c1(cell samples)\u3002<\/p>\n

\"HOT\"\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269IV(Mitochondrial respiratory chain complex IV)\uff0c\u4e5f\u7a31\u70ba\u7d30\u80de\u8272\u7d20C\u6c27\u5316\u9176(cytochrome C oxidase)\uff0c\u4e5f\u662f\u7c92\u7dda\u9ad4\u547c\u5438\u934a(mitochondrial respiratory chain)\u4e3b\u8981\u548c\u5206\u652f\u901a\u8def\u7684\u5e38\u898b\u6210\u5206\u3002\u5b83\u8ca0\u8cac\u50ac\u5316\u9084\u539f\u7d30\u80de\u8272\u7d20 C (reduced cytochrome C)\u7684\u6c27\u5316\uff0c\u4e26\u6700\u7d42\u5c07\u96fb\u5b50(electrons)\u8f49\u79fb\u5230\u6c27\u6c23(oxygen)\u4ee5\u7522\u751f\u6c34(water)\u3002CheKine\u2122 \u7c92\u7dda\u9ad4\u8907\u5408\u7269\u2163\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2 (CheKine\u2122 Micro Mitochondrial complex \u2163 Activity Assay Kit\u8ca8\u865fKTB1880)\u00a0<\/a><\/span>\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u9748\u654f\u6e96\u78ba\u3002\u9084\u539f\u578b\u7d30\u80de\u8272\u7d20 C \u5728 550nm \u8655\u5177\u6709\u7279\u5fb5\u5149\u5438\u6536\u3002\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269IV\u53ef\u50ac\u5316\u9084\u539f\u578b\u7d30\u80de\u8272\u7d20C\u6c27\u5316\u70ba\u6c27\u5316\u7d30\u80de\u8272\u7d20C(oxidized cytochrome C)\uff0c\u56e0\u6b64\uff0c550nm\u8655\u5149\u5438\u6536\u7684\u905e\u6e1b\u7387\u53ef\u53cd\u6620\u7c92\u7dda\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269IV(mitochondrial respiratory chain complex IV)\u7684\u9176\u6d3b\u6027\u3002<\/p>\n

\"HOT\"\u7dda\u7c92\u9ad4\u547c\u5438\u93c8\u8907\u5408\u9ad4V(Mitochondrial respiratory chain complex V)\uff0c\u53c8\u7a31F1F0-ATP\u5408\u9176(F1F0-ATP synthase)\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269\u548c\u57f9\u990a\u7d30\u80de\u7684\u7c92\u7dda\u9ad4\u4e2d\u3002\u8907\u5408\u7269 V \u7531\u5169\u500b\u4e9e\u57fa F1 \u548c F0 \u7d44\u6210\u3002\u5b83\u53ef\u4ee5\u5229\u7528\u547c\u5438\u93c8\u7522\u751f\u7684\u8cea\u5b50\u7684\u96fb\u5316\u5b78\u68af\u5ea6(electrochemical gradient)\u50ac\u5316ATP\u7684\u5408\u6210\uff0c\u4e5f\u53ef\u4ee5\u5728\u53ef\u9006(reversible process)\u904e\u7a0b\u4e2d\u6c34\u89e3ATP\u3002\u6b64\u5916\uff0c\u8907\u5408\u7269V(complex V)\u9084\u5b58\u5728\u65bc\u8449\u7da0\u9ad4(chloroplasts)\u3001\u7570\u990a\u7d30\u83cc(heterotrophic bacteria)\u548c\u5149\u5408\u7d30\u83cc(photosynthetic bacteria)\u4e2d\u3002 Complex V\u662f\u7c92\u7dda\u9ad4\u6c27\u5316\u78f7\u9178\u5316(mitochondrial oxidative phosphorylation)\u548c\u8449\u7da0\u9ad4(chloroplast)\u5149\u5408\u78f7\u9178\u5316\u5408\u6210ATP\u7684\u95dc\u9375\u9176\u3002Abbkine CheKine\u2122 Micro Mitochondrial complex \u2164\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(Abbkine CheKine\u2122 Micro Mitochondrial complex \u2164 Activity Detection Kit, KTB1890)<\/a><\/span>\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u9748\u654f\u6e96\u78ba\u3002\u8907\u5408\u7269\u2164\u6c34\u89e3ATP\u7522\u751fADP\u548cPi\u3002\u8907\u5408\u7269\u2164\u7684\u6d3b\u6027\u901a\u904e\u6e2c\u5b9aPi\u589e\u52a0\u7387\u4f86\u78ba\u5b9a\u3002<\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

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