{"id":37073,"date":"2022-12-13T12:36:17","date_gmt":"2022-12-13T04:36:17","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=37073"},"modified":"2022-12-27T10:51:32","modified_gmt":"2022-12-27T02:51:32","slug":"egfr%e7%aa%81%e8%ae%8a%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-detection-kit-for-egfr-mutations-bd0040","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=37073","title":{"rendered":"EGFR\u7a81\u8b8a\u6aa2\u6e2c\u8a66\u5291\u76d2 | Detection kit for EGFR Mutations, BD0040"},"content":{"rendered":"<p><strong><span style=\"color: #33cccc; font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=37073\">EGFR\u7a81\u8b8a\u6aa2\u6e2c\u8a66\u5291\u76d2 | Detection kit for EGFR Mutations, BD0040<\/a><\/span><\/strong><\/p>\n<p><img decoding=\"async\" src=\"https:\/\/www.bioworlde.com\/data\/attachment\/202108\/29\/ee33c005f31d77a4.jpg\" alt=\"bioworlde\" \/><\/p>\n<p>EGFR\u7a81\u8b8a\u6aa2\u6e2c\u8a66\u5291\u76d2 (Detection kit for EGFR Mutations, BD0040)\uff08\u87a2\u5149\u63a2\u6e2c\uff09\u63a1\u7528\u87a2\u5149\u63a2\u67e5\u6280\u8853\uff0c\u6aa2\u6e2c\u80ba\u764c\u60a3\u8005EGFR\u516d\u7a2e\u7a81\u8b8a\u985e\u578b\uff1b\u9069\u7528\u65bc\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u6216\u975e\u5c0f\u7d30\u80de\u7d44\u7e54(non small cell lung cancer )\u3002\u6aa2\u6e2c\u7d50\u679c\u53ef\u4ee5\u53cd\u6620\u6613\u745e\u6c99(iressa)\u548c\u7279\u7f85\u51f1(tarceva)\u7b49\u9776\u5411\u85e5\u7269\u7684\u6709\u6548\u6027\uff0c\u4f46\u4e0d\u7528\u65bc\u81e8\u5e8a\u8a3a\u65b7(clinical diagnosis)\u548c\u6cbb\u7642\u3002\u3010\u6aa2\u6e2c\u539f\u7406\u3011 \u8a72\u8a66\u5291\u76d2\u4f7f\u7528 TaqMan-MGB \u63a2\u91dd\u6280\u8853(TaqMan-MGB probing technology)\u6aa2\u6e2c\u516d\u500b\u7a81\u8b8a\u4f4d\u9ede\u3002 5&#8217;TaqMan-MGB \u63a2\u91dd\u7684\u672b\u7aefsigned by reporter such as FAM, VIC\u3002 MGB \u53ef\u4ee5\u589e\u5f37\u63a2\u91dd\u8207\u6a21\u677f\uff0c\u63d0\u9ad8\u63a2\u91dd\u7684 Tm\u3002\u9019\u7a2e\u5e36\u6709 MGB \u7684\u8f03\u77ed\u63a2\u982d\u53ef\u4ee5\u9054\u5230\u66f4\u9ad8\u7684Tm \u985e\u4f3c\uff0c\u4f7f\u5831\u544a\u57fa\u56e0\u66f4\u63a5\u8fd1\u731d\u6ec5\u5291\uff0c\u5f9e\u800c\u7522\u751f\u66f4\u597d\u7684\u731d\u6ec5\u6548\u679c\uff0c\u66f4\u4f4e\u7684\u87a2\u5149\u80cc\u666f\u548c\u66f4\u9ad8\u7684\u4fe1\u566a\u6bd4(signal-noise ratio)\u3002\u63a2\u6e2c\u5b8c\u6210\u7576\u5b83\u4e0d\u8207\u6a21\u677f\u7d50\u5408\u6642\uff0c\u53e6\u5916\uff0c\u7531\u5831\u544a\u57fa\u56e0\u88ab\u6dec\u6ec5\u5291\u5438\u6536\uff0c\u5c0e\u81f4\u4e0d\u767c\u87a2\u5149\u3002\u5426\u5247\uff0c\u63a2\u91dd\u5728\u8207\u6a21\u677f\u7d50\u5408\u6642\u88ab Taq \u7684 5&#8217;\u21923&#8242; \u5207\u9664\u9176\u964d\u89e3\uff0c\u5c0e\u81f4\u5831\u544a\u5206\u5b50\u548c\u731d\u6ec5\u5291\u5206\u96e2\uff0c\u7136\u5f8c\u767c\u51fa\u87a2\u5149\u51fa\u73fe\u4e86\u3002 TaqMan-MGB \u63a2\u6e2c\u65b9\u6cd5\u5177\u6709\u4e00\u4e9b\u512a\u9ede\uff0c\u4f8b\u5982\u66f4\u9ad8\u7684\u654f\u611f\u6027\u548c\u7279\u7570\u6027\u3002\u4e00\u7ba1\u591a\u91cdPCR\uff0c\u53ef\u6e1b\u5c11\u751f\u7522\u6210\u672c\u548c\u63d0\u9ad8\u6548\u7387\uff0c\u53ef\u4ee5\u901a\u904e\u66ff\u4ee3\u5be6\u73fe\u4e0d\u540c\u6ce2\u9577\u7684\u87a2\u5149\u7d44\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>Procedure :<\/p>\n<p>[Self-contained reagents] Tips, PCR 96 pore plates or eight reaction tubes without RNase and DNase. [Suitable instrument] ABI PRISM 7000\/7700\/7300, StepOnePlus Real-Time PCR System and so on. [Requirement samples] Detectable samples are as follows: 1. Serum, blood, plasma DNA. 2. Tissue including fresh or embedded in paraffin, operation sample conserved in liquid nitrogen, ethyl alcohol and RNAlater. 3. Sample from puncture and biopsy. DNA extraction: Using commercial kit to extract human genomic DNA such as QIAamp DNA Blood Mini Kit. Quantifying extracted DNA and adding less than100ng template in 20\u03bcL system. [Calibration method] In every PCR reaction, sample, positive and negative control need analyze simultaneously. 1. Preparing reagent for reaction Taking out nucleic acid reaction solution from the kit and thawing, then centrifuging at 2000rpm for 10sec. 2. Loading samples a). Adding 7.6\u03bcL PCR reaction, 10\u03bcL 2\u00d7Taq Mix and 0.4\u03bcL ROX Reference Dye to every tube. Reaction liquid can also be mixed and packaged subsequently. b). Adding 2\u03bcL DNA template, and quantifying DNA under 100ng firstly. c). Centrifuging all reagents in PCR 96 pore plates or eight reaction tubes to the bottom. 3. PCR amplification a). Putting PCR reaction plates in fluorescence quantitative PCR instrument. b). Cyclic condition setting<\/p>\n<p>For research use only, not for use in diagnostic procedures. The validity of reagents in kit is six months (Please use within the validity period ).<\/p>\n<p>Bioworld\u53f0\u7063\u4ee3\u7406\u5546-BRAND\/AGENCY\/\u4ee3\u7406\u54c1\u724c\/ Bioworld\u53f0\u7063\u4ee3\u7406\u5546<\/p>\n<p><strong><span style=\"font-size: 12pt;\"><a href=\"http:\/\/www.bioworlde.com\/\">Bioworld \u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/strong><\/p>\n<p>Bioworld\u64c1\u6709\u5c08\u696d\u7d93\u9a57\u8c50\u5bcc\u7684\u79d1\u5b78\u548c\u6280\u8853\u5c08\u696d\u4eba\u54e1\uff0c\u88fd\u9020\u548c\u92b7\u552e\u6297\u9ad4\u3002 \u6211\u5011\u76ee\u524d\u7684\u6297\u9ad4\u8986\u84cb\u7bc4\u570d\u5ee3\u6cdb\u7684\u4fe1\u865f\u8f49\u5c0e\u548c\u7d30\u80de\u6311\u9078\uff0c\u4f8b\u5982\uff1a<\/p>\n<p>1\uff09\u67d3\u8272\u8cea\u548c\u9170\u5316\u4fe1\u865f<\/p>\n<p>2\uff09MAPK\u4fe1\u865f<\/p>\n<p>3\uff09\u795e\u7d93\u5143SAPK \/ JNK\u4fe1\u865f<\/p>\n<p>4\uff09\u7d30\u80de\u51cb\u4ea1\u548cCaspase\u4fe1\u865f<\/p>\n<p>5\uff09AKT\u4fe1\u865f<\/p>\n<p>6\uff09\u8f49\u9304\u548c\u7ffb\u8b6f\u63a7\u5236<\/p>\n<p>7\uff09PKC\u4fe1\u865f<\/p>\n<p>8\uff09\u7d30\u80de\u9031\u671f\u548cDNA\u640d\u50b7\u4fe1\u865f<\/p>\n<p>9\uff09\u7d30\u80de\u9aa8\u67b6\u4fe1\u865f<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>EGFR\u7a81\u8b8a\u6aa2\u6e2c\u8a66\u5291\u76d2 | Detection kit for EGFR Mutations, BD0040  [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":31670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[136,3],"tags":[5098,5097],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37073"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=37073"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37073\/revisions"}],"predecessor-version":[{"id":37074,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/37073\/revisions\/37074"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31670"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=37073"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=37073"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=37073"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}