{"id":36600,"date":"2022-12-01T12:03:11","date_gmt":"2022-12-01T04:03:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=36600"},"modified":"2022-12-07T10:53:22","modified_gmt":"2022-12-07T02:53:22","slug":"2-nbdg-visualizing-glucose-uptake-into-living-cells-cas186689-07-6-apexbio-%e8%b2%a8%e8%99%9fb6035","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=36600","title":{"rendered":"2-NBDG\u8461\u8404\u7cd6\u4ee3\u8b1d\u67d3\u5291 | visualizing glucose uptake into living cells CAS#186689-07-6 APExBIO \u8ca8\u865fB6035"},"content":{"rendered":"

\u96f7\u5e15\u9ef4\u83cc\uff08Rapamycin\uff09<\/a>|\u00a0Romidepsin (FK228, depsipeptide)<\/a>\u00a0|\u00a0Daptomycin\u00a0<\/a>|\u00a0G-418 (80 S \u6838\u91a3\u9ad4\u5ef6\u4f38\u6291\u5236\u5291)<\/a>\u00a0<\/span><\/p>\n

\"HOT\"2-NBDG | \u00a0visualizing glucose uptake into living cells\u00a0CAS#186689-07-6 APExBIO\u00a0 \u8ca8\u865fB6035<\/a>\u00a0(10uM)<\/span><\/p>\n

fluorescent glucose analog for visualizing glucose uptake into living cells.<\/p>\n

2-NBDG \u662f\u4e00\u7a2e\u87a2\u5149\u6a19\u8a18\u7684 2-\u812b\u6c27\u8461\u8404\u91a3(2-deoxy-glucose)\u985e\u4f3c\u7269(analog)\uff0c\u53ef\u7528\u4f5c\u8a55\u4f30\u7d30\u80de\u8461\u8404\u7cd6\u4ee3\u8b1d(cellular glucose metabolism)\u7684\u793a\u8e64\u5291(tracer)\u3002\u8461\u8404\u7cd6(Glucose)\u662f\u7dad\u6301\u7d44\u7e54\u4e2d\u7d30\u80de\u6d3b\u52d5(cell activities)\u548c\u6046\u5b9a\u72c0\u614b(homeostasis)\u7684\u5fc5\u8981\u80fd\u91cf\u4f86\u6e90\u3002\u7cd6\u4ee3\u8b1d(Glucose metabolism)\u662f\u8a31\u591a\u75be\u75c5\u7684\u91cd\u8981\u9776\u6a19\uff0c\u4e26\u96a8\u8457\u75c5\u7406\u72c0\u6cc1(pathological condition)\u800c\u8b8a\u5316\uff0c\u56e0\u6b64\uff0c\u5c0d\u7cd6\u4ee3\u8b1d(glucose metabolism)\u7684\u8a55\u4f30\u53ef\u4ee5\u6210\u70ba\u75be\u75c5\u9032\u5c55\u7684\u91cd\u8981\u6307\u6a19\u30022-NBDG\u53ef\u7528\u65bc\u591a\u7a2e\u9ad4\u5916\u7d30\u80de (10uM)<\/span>\uff0c\u5982HepG2\u4eba\u809d\u764c\u7d30\u80de(HepG2 human hepatocarcinoma cells)\u3001L6\u5927\u9f20\u9aa8\u9abc\u808c\u7d30\u80de(L6 rat skeletal muscle cells)\u3001MCF-7\u4e73\u817a\u764c\u4e0a\u76ae\u7d30\u80de(MCF-7 breast cancer epithelial cells)\u548c\u661f\u5f62\u81a0\u8cea\u7d30\u80de(astrocytes)\uff0c\u4e5f\u7528\u65bc\u75be\u75c5\u6a21\u578b(disease models)\u3001\u7672\u7647\u5927\u9f20(epilepsy rat)\u3001\u9ad8\u8840\u7cd6(hyperglycemia)\u3001\u7cd6\u5c3f\u75c5(diabetes)\u6216\u5c0f\u9f20\u764c\u75c7\u7570\u7a2e\u79fb\u690d\u6a21\u578b(mouse xenograft model of cancer)\u3002<\/p>\n

2-NBDG \u901a\u904e\u8461\u8404\u7cd6\u8f49\u904b(glucose transporters)\u86cb\u767d\u9032\u5165\u7d30\u80de\uff0c\u96a8\u5f8c\u88ab\u5df1\u7cd6\u6fc0\u9176(hexokinase)\u78f7\u9178\u5316(phosphorylated)\u4e26\u88ab\u56f0\u5728\u7d30\u80de\u5167\u3002\u53ef\u4ee5\u5c0d\u7d30\u80de\u7522\u751f\u7684\u87a2\u5149\u9032\u884c\u6d41\u5f0f\u7d30\u80de\u8853\u6aa2\u6e2c(Flow cytometric detection)\uff0c\u4ee5\u6aa2\u67e5\u6d3b\u7d30\u80de\u5c0d 2-NBDG \u7684\u651d\u53d6(2-NBDG uptake)\uff0c\u4e26\u53ef\u4ee5\u4f7f\u7528\u87a2\u5149\u5fae\u5b54\u677f\u6e2c\u5b9a\u6cd5(fluorescence microplate assay)\u6e2c\u91cf\u8f49\u904b\u7684 2-NBDG \u7684\u7d30\u80de\u5167\u6fc3\u5ea6\u3002\u5b83\u4e5f\u53ef\u4ee5\u7c21\u55ae\u5730\u7528\u87a2\u5149\u6210\u50cf\u986f\u5fae\u93e1(fluorescence imaging microscopy)\u6216 CCD \u76f8\u6a5f\u6aa2\u6e2c\u3002<\/p>\n

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Cell experiment [1,2]:<\/strong><\/td>\n<\/tr>\n
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Cell lines<\/p>\n<\/td>\n

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HepG2 human hepatocarcinoma cells, L6 rat skeletal muscle cells, MCF-7 breast cancer epithelial cells<\/p>\n<\/td>\n<\/tr>\n

Preparation method<\/td>\nGeneral tips for obtaining a higher concentration: Please warm the tube at 37 \u2103 for 10 minutes and\/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20\u2103 for several months.<\/td>\n<\/tr>\n
Reacting condition<\/td>\n10 \u03bcM for 10 min<\/td>\n<\/tr>\n
Applications<\/td>\nIn HepG2 human hepatocarcinoma cells and L6 rat skeletal muscle cells, 2-NBDG concentrations higher than 0.25 mM might show a high degree of self-quenching. 2-NBDG could be used as a fluorescent indicator for direct glucose uptake measurement. In the MCF-7 breast cancer cells, 2-NBDG uptake displayed rapid uptake for the first one to five minutes, then slowed, reaching an apparent maximum uptake near 20\u201330 minutes.<\/td>\n<\/tr>\n
Animal experiment [3]:<\/strong><\/td>\n<\/tr>\n
Animal models<\/td>\nSprague\u2013Dawley male adult rat<\/td>\n<\/tr>\n
Dosage form<\/td>\n200 mg<\/td>\n<\/tr>\n
Application<\/td>\n2-NBDG can be used for localizing epileptic foci.<\/td>\n<\/tr>\n
Other notes<\/td>\nPlease test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/strong><\/p>\n