{"id":3566,"date":"2014-11-27T17:35:43","date_gmt":"2014-11-27T09:35:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=3566"},"modified":"2022-07-27T16:53:21","modified_gmt":"2022-07-27T08:53:21","slug":"cell-immortalization-abmgood","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=3566","title":{"rendered":"\u3010\u4e0d\u673d\u7d30\u80de\u3011Cell Immortalization \u76f8\u95dc\u7522\u54c1\uff0dABMgood"},"content":{"rendered":"<p style=\"text-align: justify;\">\u3000\u3000\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2\u57fa\u56e0\u548c\u6291\u764c\u57fa\u56e0\uff08p53\u3001Rb\u7b49\uff09\u7684\u4e0d\u6d3b\u5316\u76f8\u95dc\uff0c\u6703\u8a98\u5c0e\u7d30\u80de\u8655\u65bcreplicative senescence\u7684\u968e\u6bb5\u3002\u76ee\u524d\u4e5f\u6709\u7814\u7a76\u986f\u793a\u88ab\u611f\u67d3\u7684\u7d30\u80de\uff0cSV40 T anbtigen\u6703\u8a98\u5c0e\u7aef\u7c92\u9176\uff08Telomerase\uff09\u7684\u6d3b\u6027\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u8fd1\u5e74\u4f86\u548c\u7aef\u7c92\uff08telomere\uff09\u9577\u5ea6\u76f8\u95dc\u7684Telomerase Reverse Transcriptase protein (TERT)\u662f\u4e00\u7a2e\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u61c9\u7528\u3002\u5e73\u5e38\u7d30\u80de\u4e2d\uff0chTERT\u662f\u8655\u65bc\u4e0d\u6d3b\u5316\u614b\uff0c\u5fc5\u8981\u6642\u6703\u9032\u884c\u6d3b\u5316\u4fdd\u6301telomere\u7684\u9577\u5ea6\uff0c\u907f\u514d\u7d30\u80de\u767c\u751freplicative senescence\u7684\u73fe\u8c61\u3002\u67d0\u4e9b\u7d30\u80dehTERT \u904e\u5ea6\u8868\u73fe\uff08over-expression\uff09\u5c0d\u65bc\u8a98\u767c\u7d30\u80de\u4e0d\u673d\u5316\u662f\u7121\u6548\u7684\uff08\u5982\u521d\u4ee3\u4e0a\u76ae\u7d30\u80de\uff09\uff0c\u53cd\u800c\u6703\u9020\u6210\u7d30\u80de\u6b7b\u4ea1\u3002\u540c\u6642\u8868\u73fehTERT catalytic subunit\u4ee5\u53cap53\u6216RB siRNA\u5728\u4eba\u985e\u521d\u4ee3\u5375\u5de2\u4e0a\u76ae\u7d30\u80de\u6703\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u904e\u5ea6\u8868\u73feRas\u6216Myc T58A mutants\u4e5f\u6709\u521d\u4ee3\u7d30\u80de\u4e0d\u673d\u5316\u7684\u73fe\u8c61\u7522\u751f\u3002<\/p>\n<p style=\"text-align: justify;\"><span style=\"color: #008000; font-size: 12pt;\"><strong>ABM\u63d0\u4f9b\u4ee5\u4e0b\u4e0d\u673d\u7d30\u80de\u7684\u7814\u7a76\u5de5\u5177\uff1a<\/strong><\/span><\/p>\n<ul>\n<li>Gene Immortalization (Myc, p53, Rb, Ras) Reagents<\/li>\n<li>HPV E6-E7 Cell Immortalization Reagents<\/li>\n<li>SV40 T antigen Cell Immortalization Reagents<\/li>\n<li>EBV Cell Immortalization Reagents<\/li>\n<li>hTERT Cell Immortalization Reagents<\/li>\n<\/ul>\n<table>\n<tbody>\n<tr>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td style=\"text-align: center;\" width=\"100\"><strong>\u5305\u88dd<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"384\"><span style=\"text-decoration: underline;\"><a title=\"SV40 T antigen Cell Immortalization Reagents\" href=\"http:\/\/www.abmgood.com\/SV40-Cell-Immortalization.html\" target=\"_blank\"><strong>SV40\u91cd\u7d44\u75c5\u6bd2<\/strong><\/a><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G203<\/td>\n<td width=\"476\">Lenti-SV40 Lentivirus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G256<\/td>\n<td width=\"476\">Lenti-SV40T Lentivirus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G258<\/td>\n<td width=\"476\">Lenti-SV40 T+t Lentivirus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G210<\/td>\n<td width=\"476\">Adeno-SV40 Adenovirus<\/td>\n<td style=\"text-align: center;\" width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G212<\/td>\n<td width=\"476\">Retro-SV40 Retrovirus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"312\"><span style=\"text-decoration: underline;\"><a title=\"hTERT Cell Immortalization Reagents\" href=\"http:\/\/www.abmgood.com\/hTERT-Cell-Immortalization.html\" target=\"_blank\"><strong>hTERT\u91cd\u7d44\u75c5\u6bd2<\/strong><\/a><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G200<\/td>\n<td width=\"476\">Lenti-hTERT Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G201<\/td>\n<td width=\"476\">Lenti-hTERT Antisense Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G706<\/td>\n<td width=\"476\">Lenti-EF1\u03b1-hTERT Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G205<\/td>\n<td width=\"476\">Adeno-hTERT Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G208<\/td>\n<td width=\"476\">Adeno-hTERT Antisense Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">250\u03bcl<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G207<\/td>\n<td width=\"476\">Retro-E1-hTERT Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"384\"><span style=\"text-decoration: underline;\"><a title=\"Gene Immortalization (Myc, p53, Rb, Ras) Reagents\" href=\"http:\/\/www.abmgood.com\/Myc-p53-Rb-Ras-Cell-Immortalization.html\" target=\"_blank\"><strong>\u764c\u75c7\u57fa\u56e0\u76f8\u95dc\u91cd\u7d44\u75c5\u6bd2<\/strong><\/a><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G217<\/td>\n<td width=\"476\">Lenti-Myc-T58A Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G219<\/td>\n<td width=\"476\">Lenti-p53 siRNA Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G221<\/td>\n<td width=\"476\">Lenti-Ras-V12 Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G223<\/td>\n<td width=\"476\">Lenti-Rb siRNA Virus<\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" colspan=\"3\" width=\"384\"><strong>\u5176\u4ed6\u764c\u75c7\u76f8\u95dc\u91cd\u7d44\u75c5\u6bd2<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G229<\/td>\n<td width=\"476\"><span style=\"text-decoration: underline;\"><a title=\"EBV Cell Immortalization Reagents\" href=\"http:\/\/www.abmgood.com\/EBV-Cell-Immortalization.html\" target=\"_blank\">EBV Virus<\/a><\/span><\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center;\" width=\"100\">G268<\/td>\n<td width=\"476\"><span style=\"text-decoration: underline;\"><a title=\"HPV E6-E7 Cell Immortalization Reagents\" href=\"http:\/\/www.abmgood.com\/HPV-E6-E7-Cell-Immortalization.html\" target=\"_blank\">Lenti-HPV-16 E6\/E7 Virus<\/a><\/span><\/td>\n<td style=\"text-align: center;\" width=\"100\">10ml<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000\u9020\u6210\u7d30\u80de\u4e0d\u673d\u5316\u7684\u5de5\u5177\u4e2d\uff0cSV40 T antigen\u662f\u6700\u7c21\u55ae\u4e14\u6700\u5e38\u7528\u4f86\u9020\u6210\u4e0d\u673d\u5316\u7d30\u80de\u751f\u6210\u7684\u65b9\u6cd5\u3002\u591a\u6578\u7684\u75c5\u6bd2 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":3571,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[57,3],"tags":[289],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/3566"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=3566"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/3566\/revisions"}],"predecessor-version":[{"id":6597,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/3566\/revisions\/6597"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/3571"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=3566"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=3566"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=3566"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}