{"id":35539,"date":"2022-10-28T11:17:06","date_gmt":"2022-10-28T03:17:06","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=35539"},"modified":"2022-11-12T19:16:53","modified_gmt":"2022-11-12T11:16:53","slug":"%e6%ba%b6%e9%85%b6%e9%ab%94%e7%b4%b0%e8%83%9e%e5%85%a7%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-lysosomal-intracellular-activity-assay-kit%e8%b2%a8%e8%99%9f-k448-abcam-ab23462","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=35539","title":{"rendered":"\u6eb6\u9176\u9ad4\u7d30\u80de\u5167\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | Lysosomal Intracellular Activity Assay Kit\u8ca8\u865f K448 (ABCAM ab234622)"},"content":{"rendered":"

\u6eb6\u9176\u9ad4\u7d30\u80de\u5167\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | <\/strong>Lysosomal Intracellular Activity Assay Kit\u8ca8\u865f K448 (ABCAM ab234622)<\/span><\/p>\n

\"k448-c\"<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u6eb6\u9176\u9ad4(Lysosomes)\u662f\u5c0d\u5404\u7a2e\u7d30\u80de\u904e\u7a0b\u5f88\u91cd\u8981\u7684\u819c\u7d50\u5408\u7d30\u80de\u5668(membrane-bound organelles)\u3002\u5b83\u5011\u542b\u6709\u7528\u65bc\u67d0\u4e9b\u751f\u7269\u5206\u5b50\u4ee3\u8b1d\u7684\u6c34\u89e3\u9176(hydrolytic enzymes)\u3002\u7d30\u80de\u5916\u8ca8\u7269(extracellular cargo)\uff08\u4f8b\u5982\u71df\u990a\u7d20\u6bd2\u7d20, nutrients toxins\uff09\u8207\u7d30\u80de\u819c(cell membrane)\u7d50\u5408\uff0c\u96a8\u5f8c\u88ab\u8f49\u904b\u5230\u819c\u7d50\u5408\u7684\u5167\u9ad4(membrane-bound endosomes)\u4e2d\uff0c\u7531\u6eb6\u9176\u9ad4(lysosomes)\u9032\u4e00\u6b65\u964d\u89e3\uff0c\u800c\u7d30\u80de\u5167\u6210\u5206\u901a\u904e\u81ea\u566c(autophagy)\u8f49\u904b\u5230\u6eb6\u9176\u9ad4\u3002\u6eb6\u9176\u9ad4\u529f\u80fd\u969c\u7919\u8207\u8a31\u591a\u4eba\u985e\u75be\u75c5\u6709\u95dc\uff0c\u4f8b\u5982\u8870\u8001(aging)\u548c\u795e\u7d93\u9000\u884c\u6027\u75be\u200b\u200b\u75c5(neurodegenerative disease)\u3002\u96d6\u7136\u6eb6\u9176\u9ad4(lysosomes)\u7684\u7d30\u80de\u5167\u6d3b\u6027(intracellular activity)\u96e3\u4ee5\u5728\u6d3b\u7d30\u80de\u4e2d\u6e2c\u91cf\uff0c\u4f46 BioVision \u958b\u767c\u4e86\u4e00\u7a2e\u5c08\u6709\u7684\u6eb6\u9176\u9ad4\u7279\u7570\u6027\u81ea\u731d\u6ec5\u5e95\u7269(Lysosome-Specific Self-Quenched Substrate)\uff0c\u8a72\u5e95\u7269\u5177\u6709\u4f4e\u80cc\u666f\u87a2\u5149\u3001\u9ad8\u4fe1\u80cc\u666f\u6bd4\u4e14\u5c0d pH \u4e0d\u654f\u611f\u3002\u4f5c\u70ba\u5167\u541e\u8ca8\u7269\u7684\u5e95\u7269\u53ef\u4ee5\u88ab\u7d30\u80de\u5438\u6536\u4e26\u5728\u5167\u6eb6\u9176\u9ad4\u56ca\u6ce1\u5167(endo-lysosomal vesicle)\u964d\u89e3\u3002\u87a2\u5149\u4fe1\u865f\u5f9e\u81ea\u731d\u6ec5\u5e95\u7269\u4e2d\u6062\u5fa9\u3002\u964d\u89e3\u7522\u751f\u7684\u87a2\u5149\u4fe1\u865f\u8207\u7d30\u80de\u5167\u6eb6\u9176\u9ad4\u6d3b\u6027\u6210\u6b63\u6bd4\uff0c\u53ef\u4ee5\u4f7f\u7528\u87a2\u5149\u986f\u5fae\u93e1\u548c\/\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u9032\u884c\u6e2c\u91cf\u3002\u6eb6\u9176\u9ad4\u7d30\u80de\u5167\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2(Lysosomal Intracellular Activity Assay Kit)\uff08\u7d30\u80de\uff09\u5305\u62ec Cytochalasin D\uff0c\u4e00\u7a2e\u53ef\u6ef2\u900f\u7d30\u80de\u7684\u5167\u541e\u4f5c\u7528\u6291\u5236\u5291\uff0c\u7528\u4f5c\u5be6\u9a57\u5c0d\u7167\u3002\u9019\u7a2e\u6613\u65bc\u4f7f\u7528\u7684\u975e\u653e\u5c04\u6027\u8a66\u5291\u76d2\u53ef\u4ee5\u5c0d\u57f9\u990a\u7d30\u80de\u4e2d\u7684\u53bb\u6dec\u6ec5\u5e95\u7269\u9032\u884c\u6210\u50cf\u548c\u6e96\u78ba\u6e2c\u91cf\u3002<\/p>\n

\"k448-b\"<\/p>\n

\"k448\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
abID<\/th>\nab234622<\/td>\n<\/tr>\n
Cat # +Size<\/th>\nK448-50<\/td>\n<\/tr>\n
Size<\/th>\n50 assays<\/td>\n<\/tr>\n
Kit Summary<\/th>\n\u2022 Detection method- Flow cytometry and Fluorescence Microscopy
\n\u2022 Species reactivity- Mammalian
\n\u2022 Applications
\n– Measurement of Intracellular lysosomal activity
\n– Screening compounds with anti-lysosomal activity<\/td>\n<\/tr>\n
Detection Method<\/th>\nFlow Cytometry (FL1)\/ Microscopy (Ex: 488 nm)<\/td>\n<\/tr>\n
Species Reactivity<\/th>\nMammalian<\/td>\n<\/tr>\n
Applications<\/th>\n\u2022 Measurement of lysosomal intracellular activity.
\n\u2022 Elucidation of the mechanisms of endocytic pathway in living cells.
\n\u2022 Screening compounds with anti-lysosomal intracellular activity<\/td>\n<\/tr>\n
Features & Benefits<\/th>\nMeasurement of lysosomal intracellular activity.<\/td>\n<\/tr>\n
Kit Components<\/th>\n\u2022 Assay Buffer (50X)
\n\u2022 Self-Quenched Substrate
\n\u2022 Cytochalasin D (100X)<\/td>\n<\/tr>\n
Storage Conditions<\/th>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/th>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/th>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u86cb\u767d\u8cea\u5b9a\u91cf\u5546\u54c1 Coomassie blue\u86cb\u767d\u8cea\u5b9a\u91cf\u5206\u6790\u5957\u7d44<\/a> | BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2(\u4f4e\u6fc3\u5ea610-600ug\/ml)| 5\uff05\u8010\u6d17\u6ecc <\/a>| BCA\u86cb\u767d\u6e2c\u5b9a\u8a66\u5291\u76d2 (\u9084\u539f\u5291\u517c\u5bb9)<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u6eb6\u9176\u9ad4\u7d30\u80de\u5167\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2 | Lysosomal Intracellular Activity Assay […]<\/p>\n","protected":false},"author":3,"featured_media":35543,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5029],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/35539"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=35539"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/35539\/revisions"}],"predecessor-version":[{"id":37629,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/35539\/revisions\/37629"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/35543"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=35539"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=35539"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=35539"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}