{"id":35083,"date":"2022-10-20T17:13:24","date_gmt":"2022-10-20T09:13:24","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=35083"},"modified":"2024-02-16T14:35:50","modified_gmt":"2024-02-16T06:35:50","slug":"ecl%e5%86%b7%e5%85%89%e8%a9%a6%e5%8a%91-superkine-west-femto-maximum-sensitivity-substrate%e8%b2%a8%e8%99%9fbmu102-en","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=35083","title":{"rendered":"ECL\u51b7\u5149\u8a66\u5291 (Femto) | SuperKine\u2122 West Femto Maximum Sensitivity Substrate\u8ca8\u865fBMU102-EN"},"content":{"rendered":"

ECL\u51b7\u5149\u8a66\u5291 | SuperKine\u2122 West Femto Maximum Sensitivity Substrate\u8ca8\u865fBMU102-EN<\/a><\/span><\/strong><\/p>\n

\"LINE\u7fa4\u767c<\/p>\n

SuperKine\u2122 West Femto Maximum Sensitivity Substrate \u63d0\u4f9b\u4e86\u4e00\u7a2e\u9ad8\u5ea6\u654f\u611f\u7684\u986f\u8272\u6eb6\u6db2\uff0c\u53ef\u589e\u5f37\u514d\u75ab\u5b78\u5be6\u9a57\u7684\u5316\u5b78\u4fe1\u865f\u3002\u70ba\u4e86\u7372\u5f97\u6700\u4f73\u7684\u5be6\u9a57\u7d50\u679c\uff0c\u60a8\u9700\u8981\u512a\u5316\u6240\u6709\u5be6\u9a57\u56e0\u7d20\uff0c\u5305\u62ec\u6a23\u54c1\u6578\u91cf\u3001\u6297\u9ad4\u6fc3\u5ea6(antibody concentration)\u3001\u819c\u7684\u4f7f\u7528\u548c\u5c01\u9589\u5291 (blocking agents)\u3002ECL \u5e95\u7269\u7cfb\u5217(ECL substrates)\u662f\u4e00\u7cfb\u5217\u9810\u6df7\u5408(premixed)\u7684\u5373\u7528\u578b(ready-to-use)\u5316\u5b78\u767c\u5149\u8a66\u5291(chemiluminescent reagents)\uff0c\u7528\u65bc\u6aa2\u6e2c\u57fa\u65bc HRP \u7684\u86cb\u767d\u8cea(HRP-based Westerns)\u3002 SuperKine\u2122 West Femto \u6700\u5927\u9748\u654f\u5ea6\u5e95\u7269\u5c08\u9580\u91dd\u5c0d\u514d\u75ab\u5370\u8de1\u4e0a(picogram amounts) \u7684\u6297\u539f\u6aa2\u6e2c\u9032\u884c\u4e86\u512a\u5316\u3002\u5e95\u7269\u7684\u5f37\u4fe1\u865f\u8f38\u51fa\u548c\u6301\u7e8c\u6642\u9593\u53ef\u4ee5\u7528\u7167\u76f8\u6216\u5176\u4ed6\u6210\u50cf\u65b9\u6cd5\u5b8c\u7f8e\u5730\u6aa2\u6e2c(picogram amounts)\u7684\u6297\u539f\u3002<\/p>\n

\"Fig.<\/p>\n

Fig. The sample is Mouse TNF-alpha protein (PRP1113, 17KD), the primary antibody is TNF-\u03b1 Polyclonal Antibody (ABP0127, 1:2500), and the secondary antibody is HRP, Goat Anti-rabbit IgG (A21020, 1:10000). The exposure time is 30s.<\/p>\n

\"\u64cd\u4f5c\u624b\u518a2\"<\/a><\/p>\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nSuperKine\u2122 West Femto Maximum Sensitivity Substrate<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 ECL HRP Substrate Reagent A
\n\u2022 ECL HRP Substrate Reagent B<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nThe SuperKine\u2122 West Femto Maximum Sensitivity Substrate developed by Abbkine is based on the innovation and optimization of the classic peroxidase substrate system. It is used for enhanced chemiluminescence (ECL) and can directly replace other expensive ECL products without the need to renew Optimize experimental conditions.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Mix the two substrate components at a 1:1 ratio to prepare the substrate working solution.
\n\u2022 Suggest 0.1-0.2ml of SuperLumia ECL working solution for 1 cm2 size of blot membrane.
\n\u2022 Incubate blot 1-5 minutes in SuperLumia ECL Substrate working solution.
\n\u2022 Drain excess reagent. Cover blot with clear plastic wrap.
\n\u2022 Expose blot to X-ray film.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for two years at 4-8\u00b0C from date of shipment. Exposure to the sun or any other intense light can harm the working solution. Short-term exposure to typical laboratory lighting will not harm the working solution.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

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