{"id":34989,"date":"2022-10-19T16:21:14","date_gmt":"2022-10-19T08:21:14","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34989"},"modified":"2022-10-20T14:45:10","modified_gmt":"2022-10-20T06:45:10","slug":"mic-qpcr-%e2%95%91-mic-%e5%8d%b3%e6%99%82%e5%ae%9a%e9%87%8f%e8%81%9a%e5%90%88%e9%85%b6%e9%8f%88%e9%8e%96%e5%8f%8d%e6%87%89%e5%84%80","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=34989","title":{"rendered":"Mic qPCR \u2551 Mic \u5373\u6642\u5b9a\u91cf\u805a\u5408\u9176\u93c8\u9396\u53cd\u61c9\u5100"},"content":{"rendered":"<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA1111.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-35003\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA1111-300x200.jpg\" alt=\"_AAA1111\" width=\"300\" height=\"200\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA1111-300x200.jpg 300w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA1111-1024x683.jpg 1024w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a>\u00a0 \u00a0<a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA6419.jpg\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone size-medium wp-image-35005\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA6419-300x200.jpg\" alt=\"_AAA6419\" width=\"300\" height=\"200\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA6419-300x200.jpg 300w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/10\/AAA6419-1024x683.jpg 1024w\" sizes=\"(max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u95dc\u65bc<\/strong><strong>BMS<\/strong><\/span><\/p>\n<p><span style=\"font-size: 10pt;\">\u00a0 \u00a0 \u00a0 Bio Molecular Systems (BMS) \u662f\u4e00\u5bb6\u70ba\u751f\u547d\u79d1\u5b78\u8a2d\u8a08\u3001\u88fd\u9020\u548c\u92b7\u552e\u5100\u5668\u7684\u751f\u7269\u6280\u8853\u516c\u53f8\u3002 BMS\u6210\u7acb\u65bc2010\u5e74\uff0c\u7531\u524dCorbett\u751f\u547d\u79d1\u5b78\u516c\u53f8\u7684\u5275\u59cb\u4eba\u548c\u8cc7\u6df1\u4eba\u54e1\u7d44\u6210\uff0c\u5728\u958b\u767c\u5275\u65b0\u3001\u591a\u529f\u80fd\u3001\u5f37\u5927\u4e14\u6613\u65bc\u4f7f\u7528\u7684\u7522\u54c1\u65b9\u9762\u64c1\u6709\u5c08\u696d\u77e5\u8b58\uff0c\u9032\u800c\u7522\u751f\u5f37\u5927\u7684\u5ba2\u6236\u5fe0\u8aa0\u5ea6\u3002\u56e0\u70ba\u5728\u751f\u7269\u6280\u8853\u88fd\u9020\u9818\u57df\u7684\u9577\u671f\u5730\u4f4d\uff0c\u4f7f\u6211\u5011\u80fd\u5920\u5728\u6301\u7e8c\u548c\u5feb\u901f\u7684\u5167\u90e8\u5275\u65b0\u548c\u7522\u54c1\u9032\u5316\u65b9\u9762\u5efa\u7acb\u826f\u597d\u7684\u8072\u8b7d\u3002BMS\u4f4d\u65bc\u6fb3\u6d32\uff0c\u64c1\u6709\u7814\u767c\u548c\u88fd\u9020\u8a2d\u65bd\uff0c\u900f\u904e\u76f4\u63a5\u63a7\u5236\u751f\u7522\u7684\u6240\u6709\u968e\u6bb5\uff0c\u53ef\u4ee5\u512a\u5316\u8cea\u91cf\u63a7\u5236\uff0c\u6700\u5927\u9650\u5ea6\u5730\u964d\u4f4e\u9593\u63a5\u6210\u672c\u4e26\u78ba\u4fdd\u6700\u5feb\u901f\u7684\u7522\u54c1\u958b\u767c\u9031\u671f\u3002<\/span><\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/ca3UiuEDXeo\" width=\"400\" height=\"400\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u7522\u54c1\u7279\u8272<\/strong><\/span><\/p>\n<ul>\n<li>\u4e0d\u5bb9\u59a5\u5354\u7684\u5feb\u901f\uff0c40\u5206\u9418\u5373\u53ef\u5b8c\u6210\u4e00\u6b21\u6e2c\u8a66\u3002<\/li>\n<li>\u7368\u7279\u7684\u52a0\u71b1\u92c1\u584a\uff0c\u6eab\u5ea6\u5747\u52fb\u6027\u9ad8\uff0c\u512a\u826f\u7684\u6eab\u63a7\uff0c\u512a\u7570\u7684\u518d\u73fe\u6027\u3002<\/li>\n<li>\u85cd\u82bd\u7121\u7dda\u9023\u7d50\uff0c\u64f4\u5145\u6027\u9ad8\uff0c\u6700\u591a\u53ef\u4ee5\u4e00\u6b21\u9032\u884c480\u500b\u53cd\u61c9\u3002<\/li>\n<li>\u9ad8\u89e3\u6790\u5ea6\u7684Melting\u66f2\u7dda<\/li>\n<li>\u56fa\u5b9a\u7684\u5149\u5b78\u7cfb\u7d71\uff0c\u6c92\u6709\u79fb\u52d5\u90e8\u4ef6\uff0c\u7121\u9808\u4efb\u4f55\u6821\u6b63<\/li>\n<li>\u5075\u6e2c\u7ba1\u5167\u5df2\u9810\u653e\u6cb9\u5c64\uff0c\u6709\u6548\u9632\u6b62\u84b8\u767c\u548c\u51b7\u51dd\u3002<\/li>\n<li>\u4fbf\u65bc\u651c\u5e36\uff0c\u8f15\u5de7\u4e14\u4e0d\u5360\u7a7a\u9593\u3002<\/li>\n<li>\u4e00\u7cfb\u5217\u5b8c\u6574\u7684\u6559\u5b78\u5f71\u7247\u3002<\/li>\n<li>\u50f9\u683c\u512a\u7570\uff0cCP\u503c\u8d85\u9ad8\u3002<\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u9ebb\u96c0\u96d6\u5c0f\uff0c\u4e94\u81df\u4ff1\u5168<\/strong><\/span><\/p>\n<p>MIC\u4f7f\u7528\u5c08\u5229\u78c1\u611f\u61c9\u6280\u8853\u52a0\u71b1\u6a23\u54c1\u548c\u98a8\u6247\u5f37\u5236\u7a7a\u6c23\u51b7\u537b\uff0cqPCR\u53ef\u572825\u5206\u9418\u5167\u5b8c\u621035\u500b\u5faa\u74b0\u3002\u6709\u8457\u5f37\u5927\u7684\u5149\u5b78\u7cfb\u7d71\u53ef\u540c\u6642\u8b80\u53d6\u6240\u6709\u901a\u9053\uff0c\u4e26\u5feb\u901f\u7684\u5206\u6790\u3002\u6bcf\u500b\u901a\u9053\u90fd\u4f7f\u7528\u7368\u7acb\u7684\u9ad8\u5f37\u5ea6LED\u3001\u5149\u96fb\u63a2\u6e2c\u5668\u548c\u6ffe\u5149\u7247\u7d44\u7d44\u5408\u5728\u4e00\u8d77\uff0c\u4ee5\u63d0\u4f9b\u7121\u8207\u502b\u6bd4\u7684\u6aa2\u6e2c\u6027\u80fd\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u4e00\u81f4\u6027\u7684\u7d50\u679c<\/strong><\/span><\/p>\n<p>MIC\u63a1\u7528\u4e86\u7368\u7279\u7684\u65cb\u8f49\u92c1\u8f49\u5b50\uff0c\u8a72\u8f49\u5b50\u5728\u52d5\u614b\u548c\u975c\u614b\u64cd\u4f5c\u671f\u9593\u5177\u6709\u7121\u8207\u502b\u6bd4\u7684\u6eab\u5ea6\u5747\u52fb\u6027\uff0c\u4f7f\u5f97\u591a\u6b21\u904b\u884c\u7684\u91cd\u73fe\u6027\u512a\u65bc\u5728\u8a31\u591a\u5faa\u74b0\u5100\u4e0a\u904b\u884c\u7684\u55ae\u500b96\u6216384\u5b54\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u9ad8\u64f4\u5145\u6027\uff0c\u7121\u9808\u64d4\u5fc3\u8cc7\u6599\u6d88\u5931<\/strong><\/span><\/p>\n<p>MIC\u53ef\u4ee5\u900f\u904e\u85cd\u82bd\u9032\u884c\u9023\u63a5\uff0c\u591a\u500bMIC\u53ef\u4ee5\u5f9e\u540c\u4e00\u500b\u5de5\u4f5c\u7ad9\u64cd\u4f5c\uff0c\u56e0\u6b6448\u8b8a\u70ba96\uff0c96\u8b8a\u70ba192\uff0c\u6700\u591a\u53ef\u5c07\u5341\u53f0MIC\u9023\u63a5\u5230\u4e00\u53f0PC\uff0c\u9019\u610f\u5473\u8457\u53ef\u4ee5\u540c\u6642\u5206\u6790\u591a\u9054480\u500b\u6a23\u54c1\u3002\u4e5f\u4e0d\u6703\u56e0\u65b7\u958b\u9023\u63a5\u800c\u4e1f\u5931\u6578\u64da\uff0c\u6bcf\u500bMIC\u90fd\u5167\u7f6e\u6578\u64da\u5b58\u5132\uff0c\u5373\u4f7fPC\u5df2\u65b7\u958b\u9023\u63a5\uff0c\u6578\u64da\u4e5f\u4e0d\u6703\u56e0\u6b64\u6d88\u5931\uff0c\u6062\u5fa9\u9023\u63a5\u5f8c\u6703\u81ea\u52d5\u50b3\u8f38\u5230PC\u4e2d\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u5f37\u5927\u7684\u5206\u6790\u8edf\u9ad4<\/strong><\/span><\/p>\n<p>\u900f\u904eHigh Resolution Melting(HRM)\u5206\u6790\u61c9\u7528\u7a0b\u5e8f\uff0c\u53ef\u6839\u64daDNA\u6a23\u54c1\u7684Melting\u884c\u70ba\u5c0d\u5176\u9032\u884c\u5206\u6790\uff0c\u5f9e\u6e05\u695a\u5730\u8b58\u5225\u7a81\u8b8a\u3002HRM\u662f\u61c9\u7528\u7684\u5b8c\u7f8e\u5de5\u5177\uff0c\u5305\u62ec\u78ba\u5b9a\u7b49\u4f4d\u57fa\u56e0\u6d41\u884c\u7387\u3001\u96dc\u5408\u6027\u7be9\u67e5\u3001DNA\u6307\u7d0b\u5716\u8b5c\u3001DNA\u7532\u57fa\u5316\u3001\u7269\u7a2e\u9451\u5b9a\u548c\u8a08\u7b97\u9ad4\u7d30\u80de\u7372\u5f97\u6027\u7a81\u8b8a\u7684\u6bd4\u7387\u3002<\/p>\n<p><span style=\"font-size: 12pt; color: #0000ff;\"><strong>\u6559\u5b78\u9023\u7d50<\/strong><\/span><\/p>\n<p>Episode 1 Introduction to micPCR Software<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/ykQqxc9euTo\" width=\"423\" height=\"423\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 2 Creating an Assay<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/gLIhmmFxyLo\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 3 Starting a Run<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/mADnWX93GoM\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 4 Sample Editor<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/RoJbuexrNgU\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 5 Cycling Analysis<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/-Rgy0AYC51Y\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 6 Melt Analysis<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/TdNOjIubeOI\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 7 HRM Analysis<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/FpduH4H6LKc\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 8 AQ Analysis<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/zwoQPDciYzo\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>Episode 9 RQ Analysis<\/p>\n<p><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/Gis__IkGIUo\" width=\"425\" height=\"350\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<table width=\"782\">\n<tbody>\n<tr>\n<td width=\"160\">\u96fb\u6e90\u4f9b\u61c9<\/td>\n<td width=\"219\">AC \u8f38\u5165<\/td>\n<td width=\"403\">100 \u2013 240 VAC, 50\/60 Hz<\/td>\n<\/tr>\n<tr>\n<td width=\"160\">\u6eab\u63a7\u8868\u73fe<\/td>\n<td width=\"219\">\u6eab\u5ea6\u6e96\u78ba\u5ea6<\/td>\n<td width=\"403\">\u00b1 0.25\u00b0C<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u6eab\u5ea6\u5747\u52fb\u5ea6<\/td>\n<td width=\"403\">\u00b10.05\u00b0C (MIC-4+HRM)<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\"><\/td>\n<td width=\"403\">\u00b10.10\u00b0C (MIC-2 and MIC-4)<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u5347\u964d\u6eab\u901f\u7387<\/td>\n<td width=\"403\">\u5347\u6eab : 5\u00b0C\/s<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\"><\/td>\n<td width=\"403\">\u964d\u6eab : 4\u00b0C\/s<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u6eab\u5ea6\u8f38\u5165\u7bc4\u570d<\/td>\n<td width=\"403\">35 &#8211; 99\u00b0C<\/td>\n<\/tr>\n<tr>\n<td width=\"160\">\u5149\u5b78\u7cfb\u7d71<\/td>\n<td width=\"219\">\u5075\u6e2c\u5668<\/td>\n<td width=\"403\">\u5149\u96fb\u4e8c\u6975\u7ba1<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u6fc0\u767c\u5149\u6e90<\/td>\n<td width=\"403\">\u9ad8\u5f37\u5ea6LED<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u901a\u9053<\/td>\n<td width=\"403\">\u7da0 : Ex. 465 nm ; Em. 510 nm<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\"><\/td>\n<td width=\"403\">\u9ec3 : Ex. 540 nm ; Em. 570 nm<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\"><\/td>\n<td width=\"403\">\u6a58 : Ex. 585 nm ; Em. 618 nm<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\"><\/td>\n<td width=\"403\">\u7d05 : Ex. 635 nm ; Em. 675 nm<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u5075\u6e2c\u983b\u7387<\/td>\n<td width=\"403\">1s<\/td>\n<\/tr>\n<tr>\n<td width=\"160\">\u8017\u6750<\/td>\n<td width=\"219\">\u5075\u6e2c\u6578\u91cf<\/td>\n<td width=\"403\">48\u500b<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u53cd\u61c9\u9ad4\u7a4d<\/td>\n<td width=\"403\">10 \u2013 25 \u00b5L<\/td>\n<\/tr>\n<tr>\n<td width=\"160\">\u64cd\u4f5c\u74b0\u5883<\/td>\n<td width=\"219\">\u6eab\u5ea6<\/td>\n<td width=\"403\">18 &#8211; 35\u00b0C<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u76f8\u5c0d\u6ebc\u5ea6<\/td>\n<td width=\"403\">20 \u2013 80%<\/td>\n<\/tr>\n<tr>\n<td width=\"160\">\u5100\u5668\u898f\u683c<\/td>\n<td width=\"219\">\u5c3a\u5bf8(L x W x H)<\/td>\n<td width=\"403\">150 x 150 x130 mm<\/td>\n<\/tr>\n<tr>\n<td width=\"160\"><\/td>\n<td width=\"219\">\u91cd\u91cf<\/td>\n<td width=\"403\">\u7d042\u516c\u65a4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>&nbsp; \u00a0 \u00a0 \u95dc\u65bcBMS \u00a0 \u00a0 \u00a0 Bio Molecular Systems (BMS) \u662f\u4e00\u5bb6\u70ba [&hellip;]<\/p>\n","protected":false},"author":5,"featured_media":35004,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[4963,4964],"tags":[5009],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34989"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=34989"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34989\/revisions"}],"predecessor-version":[{"id":35098,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34989\/revisions\/35098"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/35004"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=34989"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=34989"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=34989"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}