{"id":34973,"date":"2022-10-19T15:04:54","date_gmt":"2022-10-19T07:04:54","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34973"},"modified":"2024-04-25T18:01:26","modified_gmt":"2024-04-25T10:01:26","slug":"%e5%b0%8f%e9%bc%a0-tnfa-%e8%85%ab%e7%98%a4%e5%a3%9e%e6%ad%bb%e5%9b%a0%e5%ad%90-%ce%b1-%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-elk-biotechnology%e8%b2%a8%e8%99%9felk1395","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=34973","title":{"rendered":"\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2 | ELK Biotechnology\u8ca8\u865fELK1395"},"content":{"rendered":"

\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1395<\/a><\/span><\/p>\n

Mouse TNFa(Tumor Necrosis Factor Alpha) ELISA Kit<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fELK1395 (Sandwich ELISA)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea615.63-1000 pg\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea66.1 pg\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0d\u6aa2\u6e2c\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1)\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3002\u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture\u3002\u4e26\u5728 450nm \u00b1 10nm \u7684\u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u7136\u5f8c\u901a\u904e\u5c07\u6a23\u54c1\u7684 OD \u8207\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u6bd4\u8f03\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2d\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2\u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a2\"<\/a><\/strong><\/p>\n

\"\"<\/p>\n

\"\u54c1\u724c\u4ecb\u7d39\"<\/p>\n

ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name:<\/td>\nMouse TNFa(Tumor Necrosis Factor Alpha) ELISA Kit<\/td>\n<\/tr>\n
Reactivity:<\/td>\nMouse<\/td>\n<\/tr>\n
Alternative Names:<\/td>\nTNF Alpha; DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2<\/td>\n<\/tr>\n
Assay Type:<\/td>\nSandwich<\/td>\n<\/tr>\n
Sensitivity:<\/td>\n6.1 pg\/mL<\/td>\n<\/tr>\n
Standard:<\/td>\n1000 pg\/mL<\/td>\n<\/tr>\n
Range:<\/td>\n15.63-1000 pg\/mL<\/td>\n<\/tr>\n
Sample Type:<\/td>\nserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids<\/td>\n<\/tr>\n
Assay Length:<\/td>\n3.5h<\/td>\n<\/tr>\n
Research Area:<\/td>\nCytokine;Tumor immunity;Infection immunity;<\/td>\n<\/tr>\n
Test principle:<\/td>\nThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Alpha(TNFa). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tumor Necrosis Factor Alpha(TNFa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Alpha(TNFa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Tumor Necrosis Factor Alpha(TNFa) in the samples is then determined by comparing the OD of the samples to the standard curve.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u71b1\u92b7\u5100\u5668\"<\/strong><\/a><\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/a> \u00a0|\u00a0 Finnpipette F3 \u5fae\u91cf\u5206\u6ce8\u5668<\/a>\u00a0| \u00a0\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/a>\u00a0|\u00a0 Genereach\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/a>\u00a0 |\u00a0\u00a0 ELISA\u5100\u5668<\/a><\/span><\/p>\n

\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n

\"\u71b1\u9580\u8a66\u5291\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u54c1\u9805<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1163<\/a><\/span><\/td>\n\u4eba TSH\uff08\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK1163<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8863<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a MDA\uff08\u4e19\u4e8c\u919b\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8860<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a CAT\uff08Catalase\u904e\u6c27\u5316\u6c2b\u9176\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8860<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8860<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a CAT\uff08Catalase\u904e\u6c27\u5316\u6c2b\u9176\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8860<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8861<\/a><\/span><\/td>\n\u6591\u99ac\u9b5aGSH\uff08\u7a40\u80f1\u7518\u80bd\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8861<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8862<\/a><\/span><\/td>\n\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8862<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1157<\/a><\/span><\/td>\n\u5c0f\u9f20IL6\uff08\u767d\u7d30\u80de\u4ecb\u7d20 6\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1157<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1395<\/a><\/span><\/td>\n\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1395<\/a><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u4fc3\u92b7\u6d3b\u52d52\"<\/strong>\u3010\u7d30\u80de\u589e\u6b96&\u6bd2\u7406&\u8870\u8001\u3011<\/a>|\u00a0DNA Damege\u640d\u50b7\/\u7d30\u80deStress\u00a0<\/a>\u00a0|\u00a0\u7d30\u80de\u51cb\u4ea1&\u7d30\u80de\u67d3\u8272<\/a>\u00a0|\u00a0\u767c\u708e\u53cd\u61c9ELISA\u5206\u6790<\/a>\u00a0|\u00a0\u81bd\u56fa\u9187\u4ee3\u8b1d\/\u4ee3\u8b1d\u8a66\u9a57<\/a>\u00a0|\u00a0\u751f\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a>|\u00a0\u6297\u9ad4\u86cb\u767d\u7d14\u5316&\u5176\u5b83<\/a>\u00a0|\u00a0<\/strong>Tag \u6a19\u7c64\u55ae\u591a\u682a\u6297\u9ad4<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1395 M […]<\/p>\n","protected":false},"author":3,"featured_media":34915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452,3],"tags":[5004],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34973"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=34973"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34973\/revisions"}],"predecessor-version":[{"id":49296,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34973\/revisions\/49296"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34915"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=34973"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=34973"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=34973"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}