{"id":34963,"date":"2022-10-19T13:33:45","date_gmt":"2022-10-19T05:33:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34963"},"modified":"2022-10-19T17:57:11","modified_gmt":"2022-10-19T09:57:11","slug":"%e6%96%91%e9%a6%ac%e9%ad%9agpx1%ef%bc%88%e7%a9%80%e8%83%b1%e7%94%98%e8%82%bd%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6-1%ef%bc%89%e6%aa%a2%e6%b8%ac-elk-biotechnology%e8%b2%a8%e8%99%9felk8862","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=34963","title":{"rendered":"\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c |ELK Biotechnology\u8ca8\u865fELK8862"},"content":{"rendered":"

\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 –<\/strong>ELK Biotechnology\u8ca8\u865fELK8862<\/a><\/span><\/p>\n

Zebrafish GPX1(Glutathione Peroxidase 1) ELISA Kit<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fELK8862 (Competitive Inhibition)\uff0c\u5075\u6e2c\u7684\u9748\u654f\u5ea61.57-100 ng\/mL\u3001\u6700\u4f4e\u9748\u654f\u5ea60.48 ng\/mL\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002\u8a72\u6aa2\u6e2c\u65b9\u6cd5\u5c0d\u6aa2\u6e2c\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u51fa\u8272\u7684\u7279\u7570\u6027\u3002ELISA\u5546\u54c1\u7684\u578b\u5f0f\u591a\u5143\uff0c \u63d0\u4f9b\u4e09\u660e\u6cbbELISA\u3001\u7af6\u722d\u578bELISA\u3002\u53ef\u61c9\u7528\u65bcserum, plasma, tissue homogenates, cell lysates, cell culture\u3002\u4e26\u5728 450nm \u00b1 10nm \u7684\u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u7136\u5f8c\u901a\u904e\u5c07\u6a23\u54c1\u7684 OD \u8207\u6a19\u6e96\u66f2\u7dda\u9032\u884c\u6bd4\u8f03\u4f86\u78ba\u5b9a\u6a23\u54c1\u4e2d\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u7684\u6fc3\u5ea6\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a2\"<\/a><\/strong><\/p>\n

ELK Biotechnology\u516c\u53f8\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u751f\u547d\u79d1\u5b78\u3001\u514d\u75ab\u7814\u7a76\u7684\u9ad8\u79d1\u6280\u751f\u7269\u4f01\u696d\uff0c\u4f4d\u65bcBiolake, Donghu New & High Technology Development Zone, Wuhan city.ELK Biotechnology\u662f\u4e00\u5bb6\u5c08\u6ce8\u65bc\u514d\u75ab\u7522\u54c1\u548c\u5206\u5b50\u751f\u7269\u5b78\u7522\u54c1\u7814\u767c\u548c\u751f\u7522\u7684\u9ad8\u79d1\u6280\u751f\u7269\u516c\u53f8\u3002\u7522\u54c1\u6db5\u84cbELISA\u8a66\u5291\u76d2\u3001\u6297\u9ad4\u3001\u57fa\u56e0\u7d44\u63d0\u53d6\u8a66\u5291\u3001\u5206\u5b50\u751f\u7269\u5b78\u8a66\u5291\u3002\u6d89\u53ca\u514d\u75ab\u5316\u5b78\u3001\u816b\u7624\u7814\u7a76\u3001\u795e\u7d93\u751f\u7269\u5b78\u3001\u7d30\u80de\u9031\u671f\u3001\u4fe1\u865f\u8f49\u5c0e\u7b49\u9818\u57df\u3002\u6211\u5011\u7684\u7522\u54c1\u6709\u56b4\u683c\u7684\u7814\u767c\u548c\u8cea\u91cf\u6aa2\u6e2c\u6d41\u7a0b\u3002\u6bcf\u500b\u7522\u54c1\u90fd\u6709\u5b8c\u6574\u7684\u7814\u767c\u3001\u751f\u7522\u548c\u8cea\u6aa2\u8a18\u9304\u3002\u7d93\u9a57\u8c50\u5bcc\u7684\u7814\u767c\u4eba\u54e1\uff0c\u5b8c\u5584\u7684\u8cea\u6aa2\u652f\u6301\u90e8\u9580\uff0c\u56b4\u683c\u7684\u6aa2\u6e2c\u6d41\u7a0b\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u8cea\u91cf\u53ef\u9760\u7684\u79d1\u7814\u7522\u54c1\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name:<\/td>\nZebrafish GPX1(Glutathione Peroxidase 1) ELISA Kit<\/td>\n<\/tr>\n
Reactivity:<\/td>\nZebrafish<\/td>\n<\/tr>\n
Alternative Names:<\/td>\nGSHPX1; Cellular glutathione peroxidase<\/td>\n<\/tr>\n
Assay Type:<\/td>\nSandwich<\/td>\n<\/tr>\n
Sensitivity:<\/td>\n7.2 pg\/mL<\/td>\n<\/tr>\n
Standard:<\/td>\n1000 pg\/mL<\/td>\n<\/tr>\n
Range:<\/td>\n15.63-1000 pg\/mL<\/td>\n<\/tr>\n
Sample Type:<\/td>\nSerum, plasma and other biological fluids.<\/td>\n<\/tr>\n
Assay Length:<\/td>\n3.5h<\/td>\n<\/tr>\n
Research Area:<\/td>\nEnzyme & Kinase;Metabolic pathway;<\/td>\n<\/tr>\n
Test principle:<\/td>\nThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutathione Peroxidase 1(GPX1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glutathione Peroxidase 1(GPX1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutathione Peroxidase 1(GPX1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm \u00b1 10nm. The concentration of Glutathione Peroxidase 1(GPX1) in the samples is then determined by comparing the OD of the samples to the standard curve.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u71b1\u9580\u8a66\u5291\"<\/p>\n

\u4e7e\u6d74\u52a0\u71b1\u6c34\u6d74\u69fd<\/strong><\/a> \u00a0|\u00a0 <\/strong>Finnpipette F3 <\/strong>\u5fae\u91cf\u5206\u6ce8\u5668<\/strong><\/a>\u00a0| \u00a0<\/strong>\u8a66\u7ba1\u632f\u76ea\u5668 Vortex Mixer\u00a0<\/strong><\/a>\u00a0|\u00a0 <\/strong>Genereach<\/strong>\u8ff7\u4f60\u578b\u5fae\u91cf\u96e2\u5fc3\u6a5f<\/strong><\/a>\u00a0 |\u00a0\u00a0 <\/strong>ELISA<\/strong>\u5100\u5668<\/strong><\/a><\/span><\/p>\n

\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a><\/p>\n

\"\u71b1\u9580\u8a66\u5291\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u54c1\u9805<\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1163<\/a><\/span><\/td>\n\u4eba TSH\uff08\u4fc3\u7532\u72c0\u817a\u6fc0\u7d20\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK1163<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8863<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a MDA\uff08\u4e19\u4e8c\u919b\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8860<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a CAT\uff08Catalase\u904e\u6c27\u5316\u6c2b\u9176\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8860<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8860<\/a><\/span><\/td>\n\u6591\u99ac\u9b5a CAT\uff08Catalase\u904e\u6c27\u5316\u6c2b\u9176\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8860<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8861<\/a><\/span><\/td>\n\u6591\u99ac\u9b5aGSH\uff08\u7a40\u80f1\u7518\u80bd\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8861<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK8862<\/a><\/span><\/td>\n\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0–<\/u>ELK Biotechnology\u8ca8\u865fELK8862<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1157<\/a><\/span><\/td>\n\u5c0f\u9f20IL6\uff08\u767d\u7d30\u80de\u4ecb\u7d20 6\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1157<\/a><\/span><\/td>\n<\/tr>\n
\u8ca8\u865fELK1395<\/a><\/span><\/td>\n\u5c0f\u9f20 TNFa (\u816b\u7624\u58de\u6b7b\u56e0\u5b50-\u03b1) \u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0|\u00a0ELK Biotechnology\u8ca8\u865fELK1395<\/a><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

\u6591\u99ac\u9b5aGPX1\uff08\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176 1\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2 –ELK Biotechnology\u8ca8\u865fELK […]<\/p>\n","protected":false},"author":3,"featured_media":34915,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[5005],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34963"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=34963"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34963\/revisions"}],"predecessor-version":[{"id":34982,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/34963\/revisions\/34982"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/34915"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=34963"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=34963"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=34963"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}