{"id":34067,"date":"2022-09-12T10:25:05","date_gmt":"2022-09-12T02:25:05","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=34067"},"modified":"2023-10-11T17:37:16","modified_gmt":"2023-10-11T09:37:16","slug":"abbkine%e7%b4%b0%e8%83%9e%e6%9f%93%e5%8a%91-cfdadapi%e7%b4%b0%e8%83%9e%e6%9f%93%e8%89%b2-abbkine%e6%9f%93%e5%8a%91","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=34067","title":{"rendered":"ABBKINE\u7d30\u80de\u67d3\u5291 | CFDA\/DAPI\u7d30\u80de\u67d3\u8272-Abbkine\u67d3\u5291"},"content":{"rendered":"

\u87a2\u5149\u67d3\u6599 (Fluorescent Dyes)-5(6)-CFDA, SE<\/a><\/span><\/span><\/strong><\/span><\/p>\n

5(6)-CFDA, SE\u00a0| \u00a05-(and-6)-Carboxyfluorescein diacetate\u00a0\u8ca8\u865fBMD0066<\/a><\/span><\/strong><\/span>
\n\u7d30\u80de\u67d3\u8272(Cell staining)\u662f\u8ffd\u8e2a\u8907\u96dc\u74b0\u5883\u4e2d\u7d30\u80de\u7684\u91cd\u8981\u5de5\u5177\uff0c\u5ee3\u6cdb\u61c9\u7528\u65bc\u7d30\u80de\u9077\u79fb(cell migration)\u3001\u50b7\u53e3\u7652\u5408(wound healing)\u548c\u5e79\u7d30\u80de\u5206\u5316\u7814\u7a76(stem cell differentiation studies)\u3002\u7d30\u80de\u904b\u52d5(Cell movement)\u548c\u5b9a\u4f4d\u7814\u7a76(localization studies )\u9700\u8981\u7279\u6b8a\u7684\u63a2\u91dd\u67d3\u6599\uff0c\u5fc5\u9808\u5c0d\u6d3b\u7d30\u80de\u7121\u6bd2(non-toxic )\uff0c\u540c\u6642\u6709\u591a\u7a2e\u87a2\u5149\u984f\u8272\u53ef\u4f9b\u9078\u64c7\uff0c\u4ee5\u5339\u914d\u4e0d\u540c\u7684\u5100\u5668\u6fc0\u5149\u6ce2\u9577\u548c\u6ffe\u5149\u7247\uff0c\u53ef\u4ee5\u6a19\u8a18\u6297\u9ad4\u6216\u5176\u4ed6\u7d30\u80de\u5206\u6790\u6a19\u8a18\u63a2\u91dd\u5171\u67d3\u3002Abbkine\u63d0\u4f9b\u8010\u5149\u6027\u5f37\u3001\u6bd2\u6027\u4f4e\u7684\u87a2\u5149\u67d3\u6599\uff0c\u5982\uff1a\u9b3c\u7b46\u74b0\u80bd(phalloidin)\u3001Cy5\u3001Cy3\u67d3\u6599\u3001Biotin SE\u3001DAPI\u7b49\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"5(6)-CFDA, SE\uff08\u5168\u540d\uff1a5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester\uff0c\u4e5f\u7a31\u70ba CFSE\uff09\u662f\u4e00\u7a2e\u6709\u7528\u7684\u87a2\u5149\u793a\u8e2a\u5291(fluorescent tracer)\uff0c\u53ef\u4ee5\u88ab\u52d5\u64f4\u6563\u5230\u7d30\u80de\u4e2d\u4e26\u5171\u50f9\u6a19\u8a18\u7d30\u80de\u5167\u86cb\u767d\u8cea(intracellular proteins)\uff0c\u5f9e\u800c\u5c0e\u81f4\u9577\u671f\u7d30\u80de\u6a19\u8a18\u3002\u8a72\u8a66\u5291\u672c\u8eab\u662f\u7121\u8272\u7121\u87a2\u5149\u7684\uff0c\u4f46\u4e00\u65e6\u88ab\u7d30\u80de\u5167\u916f\u9176(intracellular esterases)\u6c34\u89e3\uff0c\u5c31\u6703\u8b8a\u6210\u4eae\u7da0\u8272\u87a2\u5149\u3002<\/p>\n

\"abbkine02\"<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n
Product name<\/strong><\/td>\n5(6)-CFDA, SE<\/a><\/td>\n<\/tr>\n
CAS Number<\/strong><\/td>\n150347-59-4<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC29<\/sub>H19<\/sub>NO11<\/sub><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\n5(6)-CFDA, SE,5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester; CFSE; BMD00066<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Product Properties<\/span><\/strong><\/h3>\n\n\n\n\n\n\n\n\n\n
Formulation<\/strong><\/td>\nWhite solid or Pale yellow solid<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 \u03bbEX\/\u03bbEm: 494\/521\u3002\uff08pH=7\uff09<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n557.47<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Soluble in DMSO<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStore at -20\u00b0C and protect from light. Product is stable for at least 12\u00a0months from date of receipt if stored as recommended.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

DAPI Staining Solution \u7c89\u72c0 (DNA staining)\u00a0|\u00a0\u8ca8\u865fBMD00063<\/a><\/span><\/strong><\/span><\/p>\n

\u7522\u54c1<\/strong>\u63cf\u8ff0\uff1a<\/strong>\u00a0DAPI\u662f\u4e00\u7a2e\u6d41\u884c\u7684\u85cd\u8272\u87a2\u5149DNA\u67d3\u6599(blue fluorescent DNA dye)\u3002\u8a72\u67d3\u6599\u8207dsDNA\u7684\u7d50\u5408\uff0c\u87a2\u5149\u589e\u5f37\u7d0420\u500d\u3002\u7531\u65bcDAPI\u7a7f\u904e\u5b8c\u6574\u7684\u7d30\u80de\u819c\uff0c\u56e0\u6b64\u53ef\u7528\u65bc\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de\u3002DAPI\u901a\u5e38\u7528\u65bc\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\uff0c\u67d3\u8272\u5f8c\u5c07\u4f7f\u7528\u87a2\u5149\u986f\u5fae\u93e1(fluorescence microscope)\u6216\u6d41\u5f0f\u7d30\u80de\u5100(flow cytometer)\u9032\u884c\u6aa2\u6e2c\u3002DAPI\u7684\u85cd\u8272\u767c\u5c04\u4fbf\u65bc\u9032\u884c\u591a\u91cd\u5206\u6790\uff0c\u56e0\u70baDAPI\u8207\u7da0\u8272\u87a2\u5149\u5206\u5b50\uff08\u5982FITC\u548c\u7da0\u8272\u87a2\u5149\u86cb\u767d\uff08GFP\uff09\u6216\u7d05\u8272\u87a2\u5149\u67d3\u6599\uff08\u5982Texas Red\uff09\u4e4b\u9593\u5e7e\u4e4e\u6c92\u6709\u87a2\u5149\u91cd\u758a\u3002<\/p>\n

\"\"<\/p>\n

Fig. DAPI structure formula<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nDAPI<\/a><\/td>\n<\/tr>\n
CAS Number<\/strong><\/td>\n28718-90-3<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC16<\/sub>H17<\/sub>Cl2<\/sub>N5<\/sub><\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nDAPI,BMD00063<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\n\u00a0Yellow powder<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 \u03bbEx\/\u03bbEm: 360\/460 nm (with DNA)<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n350.25<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Soluble in H2O<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStore at -20\u00b0C and protect from light. Product is stable for at least 12\u00a0months from date of receipt if stored as recommended.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nDAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement. DAPI is often applied to apoptosis detection, which will use fluorescence microscope or flow cytometer after staining.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nDAPI,BMD00063<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u5f15\u7528\u6587\u737b<\/p>\n

Synthesis and Evaluation of a Chitosan Oligosaccharide-Streptomycin Conjugate against Pseudomonas aeruginosa Biofilms<\/a><\/p>\n

Ruilian Li, Xianghua Yuan, Jinhua Wei, et al. Mar Drugs. 2019 Jan 10;17(1). pii: E43.<\/p>\n

AbFluor\u2122 488-Phalloidin\u00a0(Ex\/Em = 490\/515 nm)<\/p>\n

F-actin\u808c\u52d5\u86cb\u767d\u67d3\u8272\u5957\u7d44 (AbFluor\u2122 488-Phalloidin) | \u8ca8\u865fBMD00082<\/a><\/span><\/strong><\/span><\/p>\n

F-actin\u808c\u52d5\u86cb\u767d\u662f\u4e00\u7a2e\u5927\u7d0442 kDa\u7684\u86cb\u767d\u8cea\uff0c\u5e7e\u4e4e\u5728\u6240\u6709\u771f\u6838\u7d30\u80de\u4e2d\u90fd\u6709\u767c\u73fe\uff0c\u5b83\u4e5f\u662f\u6700\u9ad8\u5ea6\u4fdd\u5b88\u7684\u86cb\u767d\u8cea\u4e4b\u4e00\u3002\u808c\u52d5\u86cb\u767d\u7684\u55ae\u9ad4\u70ba\u7403\u5f62\u5206\u5b50\uff0c\u7a31\u70ba\u7403\u5f62\u808c\u52d5\u86cb\u767dG-actin\uff0c\u5b83\u7684\u591a\u805a\u9ad4\u7a31\u70ba\u7e96\u7dad\u5f62\u808c\u52d5\u86cb\u767dF-actin\u3002\u9019\u5169\u8005\u5c0d\u65bc\u7d30\u80de\u5206\u88c2\u904e\u7a0b\u4e2d\u7d30\u80de\u7684\u79fb\u52d5\u548c\u6536\u7e2e\u7b49\u91cd\u8981\u7684\u7d30\u80de\u529f\u80fd\u90fd\u662f\u5fc5\u4e0d\u53ef\u5c11\u7684\u3002AbFluor\u2122 488-Phalloidin\u9078\u64c7\u6027\u5730\u7d50\u5408F-actins, \u5b83\u6bd4\u6c1f\u897f\u6797-\u9130\u82ef\u4e8c\u7532a)\u7684\u6297\u6027\u8981\u9ad8\u5f97\u591a\u3002Phalloidin\u884d\u751f\u7269 \u662f\u6a19\u8a18\u3001\u8b58\u5225\u548c\u5b9a\u91cf\u7532\u919b\u56fa\u5b9a(formaldehyde-fixed )\u548c\u6ef2\u900f\u7d44\u7e54\u90e8\u5206( permeabilized tissue sections)\u4e2d\u7684F-actin\u7684\u65b9\u4fbf\u63a2\u91dd,\u53ef\u5728\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u67d3\u8272F-actin\u6216cell-free experiments at nanomolar levels\u3002<\/p>\n

\u9130\u82ef\u4e8c\u7532\u82ef\u7532\u985e\u6bd2\u7d20(Phalloidin )<\/span><\/strong><\/span><\/p>\n

Phalloidin \u70ba\u4e00\u7a2e\u5e36\u6709\u6bd2\u6027\u7684\u80dc\u80bd\uff0c\u5177\u6709\u8207 F-actin \u5c08\u4e00\u6027\u7d50\u5408\u7684\u7279\u6027\u3002\u5c6c\u65bc\u4e00\u985e\u7a31\u70baphallo\u6bd2\u7d20(phallotoxins)\u7684\u6bd2\u7d20,\u9019\u4e9b\u6bd2\u7d20\u8207\u6b7b\u4ea1\u5e3d\u8611\u83c7(\u963f\u99ac\u5c3c\u5854phalloides)\u5206\u96e2\u3002\u5b83\u662f\u4e00\u7a2e\u4e8c\u74b0\u80bd(bicyclic peptide ),\u7279\u5225\u8207F-actin\u7d50\u5408\u3002\u56e0\u6b64,\u4f7f\u7528\u87a2\u5149\u67d3\u6599\u6a19\u8a18\u7684\u9130\u82ef\u4e8c\u7532\u4e8c\u7d20\u7d20,\u53ef\u4ee5\u975e\u5e38\u65b9\u4fbf\u5730\u7814\u7a76F-actin\u7684\u5206\u4f48\u3002\u5728\u566c\u83cc\u7d20\u5167\u90e8,\u534a\u80f1\u6c28\u9178\u548c\u8272\u6c28\u9178\u4e4b\u9593\u6709\u4e00\u500b\u4e0d\u5c0b\u5e38\u7684\u786b\u919a\u6a4b,\u53ef\u4ee5\u5f62\u6210\u4e00\u500b\u5167\u74b0\u7d50\u69cb\u3002\u7576pH\u5347\u9ad8\u6642,\u786b\u919a\u88ab\u5207\u5408,\u9130\u82ef\u4e8c\u7532\u9178\u916f\u5931\u53bb\u5c0d\u4f5c\u7528\u7d20\u7684\u89aa\u548c\u529b\u3002\u6a19\u5b9a\u6709\u87a2\u5149\u67d3\u6599\u7684 Phalloidin \u8a66\u5291\u7522\u54c1\uff0c\u80fd\u5920\u5354\u52a9\u7814\u7a76\u4eba\u54e1\u7cbe\u78ba\u638c\u63e1\u7d30\u80de\u5167 F-actin \u7684\u5206\u5e03\u60c5\u5f62\u3001\u4e86\u89e3 F-actin \u8868\u73fe\u91cf\u8b8a\u5316\u3002
\n\u7522\u54c1\u4ecb\u7d39:<\/strong>\u00a0AbFluor\u2122 F-actin 488-Phalloidin\u67d3\u8272\u8a66\u5291\u76d2(Gree Fluorescence, \u7da0\u8272\u87a2\u5149)\u4f7f\u7528\u7da0\u8272\u87a2\u5149(Ex\/Em = 490\/515 nm)\u9130\u82ef\u4e8c\u7532\u7d20\u5076\u806f,\u9078\u64c7\u6027\u5730\u7d50\u5408F-actins,\u5176\u5149\u7a69\u5b9a\u6027\u9060\u9060\u9ad8\u65bc\u5176\u4ed6\u6c1f\u7d20-\u566c\u83cc\u7d20\u5076\u806f(fluorescein-phalloidin conjugates)\u3002Phalloidin\u884d\u751f\u7269(fluorescein-phalloidin conjugates.)\u662f\u6a19\u8a18\u3001\u8b58\u5225\u548c\u5b9a\u91cf\u7532\u919b\u56fa\u5b9a\u548c\u6ef2\u900f\u7d44\u7e54\u90e8\u5206\u4e2d\u7684F-actin\u63a2\u91dd,\u53ef\u5728\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u67d3\u8272F-actin\u6216cell-free experiments at nanomolar levels\u3002<\/p>\n