{"id":33846,"date":"2022-08-24T15:59:20","date_gmt":"2022-08-24T07:59:20","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33846"},"modified":"2023-07-06T11:12:01","modified_gmt":"2023-07-06T03:12:01","slug":"gshgssg%e6%aa%a2%e6%b8%ac%e5%a5%97%e7%b5%84-gshgssg-ratio-assaycatalog-%e8%b2%a8%e8%99%9f8558","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33846","title":{"rendered":"GSH\/GSSG\u6aa2\u6e2c\u5957\u7d44 | GSH\/GSSG Ratio Assay Sciencell\u8ca8\u865f8558"},"content":{"rendered":"

GSH\/GSSG\u6aa2\u6e2c\u5957\u7d44 | GSH\/GSSG Ratio Assay Sciencell\u8ca8\u865f8558<\/span><\/strong><\/span><\/a><\/span><\/p>\n

\u5075\u6e2c\u7bc4\u570d0.195-6.25 uM<\/p>\n

\u7a40\u80f1\u7518\u80bd(Glutathione) \u662f\u4e00\u7a2e\u4e09\u80bd(tripeptide)\uff08g-glutamylcysteinylglycine , g-\u8c37\u6c28\u9170\u534a\u80f1\u6c28\u9170\u7518\u6c28\u9178\uff09\uff0c\u4ee5\u9084\u539f\uff08reduced GSH\uff09\u548c\u6c27\u5316\uff08GSSG\uff09\u72c0\u614b\u5b58\u5728\u3002\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u50ac\u5316\u7531 GSH \u5f62\u6210 GSSG\uff0c\u7a40\u80f1\u7518\u80bd\u9084\u539f\u9176\u5c07 GSSG \u518d\u5faa\u74b0\u70ba GSH\u3002\u96a8\u8457\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u6c34\u5e73\u7684\u589e\u52a0\uff0c\u7d30\u80de\u5167 GSSG \u6703\u7a4d\u7d2f\uff0cGSH\/GSSG \u6bd4\u7387\u6703\u964d\u4f4e\u3002\u56e0\u6b64\uff0c\u6e2c\u5b9a GSH\/GSSG \u6bd4\u503c\u662f\u7d30\u80de\u548c\u7d44\u7e54\u4e2d\u6c27\u5316\u61c9\u6fc0\u7684\u6709\u7528\u6307\u6a19\u3002\u9019\u7a2e\u6bd4\u8272\u6e2c\u5b9a\u57fa\u65bc 5′, 5′-\u4e8c\u786b\u4ee3\u96d9 2-\u785d\u57fa\u82ef\u7532\u9178 (DTNB) \u548c GSH \u4e4b\u9593\u7684\u53cd\u61c9\u5f62\u6210 TNB\uff0c\u5176\u5728 412 nm \u8655\u8868\u73fe\u51fa\u6700\u5927\u5438\u5149\u5ea6\u3002\u5438\u5149\u5ea6\u7684\u5f37\u5ea6\u8207\u6a23\u54c1\u4e2d\u7684 GSH \u6c34\u5e73\u6210\u6b63\u6bd4\u3002GSH\/GSSG \u6bd4\u7387\u6e2c\u5b9a\u8a66\u5291\u76d2\u53ef\u6e2c\u91cf\u4e0d\u540c\u985e\u578b\u6a23\u54c1\uff08\u5982\u7d44\u7e54\u3001\u7d30\u80de\u88c2\u89e3\u7269\u548c\u8840\u6f3f\uff09\u4e2d GSH \u548c GSSG \u4e4b\u9593\u7684\u6bd4\u7387\u3002\u672c\u7522\u54c1\u50c5\u7528\u65bc\u7814\u7a76\u76ee\u7684\uff0c\u4e0d\u5f97\u7528\u65bc\u52d5\u7269\u3001\u4eba\u985e\u6216\u8a3a\u65b7\u7a0b\u5e8f\u3002<\/p>\n

GSH\/GSSH\u7522\u54c1\u5167\u5bb9\u7269<\/span><\/strong><\/span><\/p>\n

<\/p>\n

GSH\/GSSHvfgr wd qmv mgbuu\u00a0<\/span><\/strong><\/span><\/p>\n

<\/p>\n

GSH\/GSSH\u539f\u7406\u53ca\u6fc3\u5ea6\u8a08\u7b97<\/span><\/strong><\/span><\/p>\n

Glutathione is a tripeptide (g-glutamylcysteinylglycine) and exists in reduced (GSH) and oxidized (GSSG) states\u3002Glutathione peroxidase catalyzes the formation of GSSG from GSH, and glutathione reductase recycles GSSG to GSH. With increased levels of oxidative stress, intracellular GSSG will accumulate, and the GSH\/GSSG ratio will decrease. Therefore, the determination of the GSH\/GSSG ratio is a useful indicator of oxidative stress in cells and tissues. This colorimetric assay is based on the reaction between 5\u2019, 5\u2019-Dithiobis 2- nitrobenzoic acid (DTNB) and GSH to form TNB, which exhibits maximum absorbance at 412 nm. The intensity of the absorbance is proportional to the GSH level in the sample\u3002<\/p>\n

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\"SCIENCELL<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n\n\n\n\n\n\n\n\n\n
Catalog No.<\/strong><\/td>\n8558<\/td>\n<\/tr>\n
Country of Manufacture<\/strong><\/td>\nUnited States<\/td>\n<\/tr>\n
Product Code<\/strong><\/td>\nGSH\/GSSG<\/td>\n<\/tr>\n
Size\/Quantity<\/strong><\/td>\n100 Tests<\/td>\n<\/tr>\n
Product Use<\/strong><\/td>\nThis product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.<\/td>\n<\/tr>\n
Storage<\/strong><\/td>\nStore in -20\u00b0C<\/td>\n<\/tr>\n
Shipping Info<\/strong><\/td>\nDry ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CAT. NO.<\/th>\nCODE<\/th>\nDESCRIPTION<\/th>\n<\/tr>\n
8008<\/td>\nCol<\/td>\nCollagen I Cell Adhesion Assay<\/a><\/td>\n<\/tr>\n
8018<\/td>\nFibro<\/td>\nFibronectin Cell Adhesion Assay<\/a><\/td>\n<\/tr>\n
8028<\/td>\nMT10<\/td>\nMTT Cell Viability & Proliferation Assay<\/a><\/td>\n<\/tr>\n
8038<\/td>\nWST<\/td>\nWST-1 Cell Viability & Proliferation Assay<\/a><\/td>\n<\/tr>\n
8058<\/td>\nCSA<\/td>\nCell Senescence Assay<\/a><\/td>\n<\/tr>\n
8078<\/td>\nLDH<\/td>\nLDH Cytotoxicity Assay<\/a><\/td>\n<\/tr>\n
8088<\/td>\nTUNEL<\/td>\nColorimetric TUNEL Apoptosis Assay<\/a><\/td>\n<\/tr>\n
8138<\/td>\nCSK<\/td>\nLive\/Dead Cell Staining Kit<\/a><\/td>\n<\/tr>\n
8158<\/td>\nIVTFA<\/td>\nIn Vitro Tube Formation Assay<\/a><\/td>\n<\/tr>\n
8178<\/td>\nC3DGK<\/td>\nCollagen I-3D Gelling Kit<\/a><\/td>\n<\/tr>\n
8188<\/td>\nCCCSCK<\/td>\nCollagen I-Cell Culture Surface Coating Kit<\/a><\/td>\n<\/tr>\n
8208<\/td>\nMYCO<\/td>\nMycoplasma PCR Detection Kit<\/a><\/td>\n<\/tr>\n
8228<\/td>\nCAS<\/td>\nCaspase-3 Assay<\/a><\/td>\n<\/tr>\n
8808<\/td>\n3D-GSP<\/td>\n3D Gel Staining Prep Kit<\/a><\/td>\n<\/tr>\n
8988<\/td>\nHLCC<\/td>\nHeLa Cell Contamination Detection Kit<\/a><\/td>\n<\/tr>\n
GK801<\/td>\nAHKRQ<\/td>\nAbsolute Human Kringle Repeats Quantification qPCR Assay Kit<\/a><\/td>\n<\/tr>\n
GK820<\/td>\nHSDQ<\/td>\nGeneQuery\u2122 Human Biological Sex Determination qPCR Assay Kit (HSDQ)<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
CAT. NO.<\/th>\nCODE<\/th>\nDESCRIPTION<\/th>\n<\/tr>\n
8118<\/td>\nMGPA<\/td>\nMalachite Green Phosphate Assay<\/a><\/td>\n<\/tr>\n
8128<\/td>\nCCA<\/td>\nColorimetric Calcium Assay<\/a><\/td>\n<\/tr>\n
8308<\/td>\nLAC<\/td>\nL-Lactate Assay<\/a><\/td>\n<\/tr>\n
8338<\/td>\nGLU<\/td>\nGlutamate Assay<\/a><\/td>\n<\/tr>\n
8368<\/td>\nNAD<\/td>\nNAD\/NADH Assay<\/a><\/td>\n<\/tr>\n
8388<\/td>\nPYR<\/td>\nPyruvate Assay<\/a><\/td>\n<\/tr>\n
8398<\/td>\nG6P<\/td>\nGlucose-6-phosphate Assay<\/a><\/td>\n<\/tr>\n
8418<\/td>\nGL<\/td>\nGlucose Assay<\/a><\/td>\n<\/tr>\n
8438<\/td>\nUS<\/td>\nUric Acid Assay<\/a><\/td>\n<\/tr>\n
8448<\/td>\nIR<\/td>\nIron Assay<\/a><\/td>\n<\/tr>\n
8468<\/td>\nGLY<\/td>\nGlycerol Assay<\/a><\/td>\n<\/tr>\n
8498<\/td>\nTG<\/td>\nTriglyceride Assay<\/a><\/td>\n<\/tr>\n
8518<\/td>\nTGA<\/td>\nTotal Glutathione Assay<\/a><\/td>\n<\/tr>\n
8528<\/td>\nAA<\/td>\nAmmonia Assay<\/a><\/td>\n<\/tr>\n
8558<\/td>\nGSH\/GSSG<\/td>\nGSH\/GSSG Ratio Assay<\/a><\/td>\n<\/tr>\n
8588<\/td>\nCho<\/td>\nCholine Assay<\/a><\/td>\n<\/tr>\n
8648<\/td>\nMal<\/td>\nMalate Assay<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

GSH\/GSSG\u6aa2\u6e2c\u5957\u7d44 | GSH\/GSSG Ratio Assay Sciencell\u8ca8\u865f8558 \u5075\u6e2c\u7bc4 […]<\/p>\n","protected":false},"author":3,"featured_media":33847,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5625],"tags":[4911],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33846"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33846"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33846\/revisions"}],"predecessor-version":[{"id":43692,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33846\/revisions\/43692"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33847"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33846"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33846"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}