{"id":33614,"date":"2022-08-03T15:34:39","date_gmt":"2022-08-03T07:34:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33614"},"modified":"2022-09-02T10:27:19","modified_gmt":"2022-09-02T02:27:19","slug":"%e8%b1%ac%e6%8a%97%e5%bc%93%e5%bd%a2%e9%ab%94%e7%97%85-igg-%e6%8a%97%e9%ab%94-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fae64451pi","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33614","title":{"rendered":"\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5 (IgG) \u6297\u9ad4 ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE64451PI"},"content":{"rendered":"

\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5<\/strong> (IgG) <\/strong>\u6297\u9ad4<\/strong> ELISA <\/strong>\u8a66\u5291\u76d2<\/strong> | <\/strong>\u8ca8\u865f<\/strong>AE64451PI<\/a><\/span><\/p>\n

Pig Anti-toxoplasmosis (IgG) Antibody ELISA Kit
\n\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5<\/strong> (IgG) <\/strong>\u6297\u9ad4<\/strong> ELISA <\/strong>\u8a66\u5291\u76d2<\/strong> Competitive ELISA | \u8ca8\u865fAE64451PI\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Abebio \u662f\u4e00\u5bb6\u9818\u5148\u7684\u751f\u7269\u6280\u8853\u516c\u53f8\uff0c\u5c08\u9580\u751f\u7522\u9ad8\u8cea\u91cf\u7684\u751f\u7269\u7522\u54c1\uff0c\u5982\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u548c ELISA \u8a66\u5291\u76d2\u3002Abebio \u751f\u7522\u6578\u4ee5\u5343\u8a08\u7684 ELISA \u8a66\u5291\u76d2\uff0c\u7528\u65bc\u6aa2\u6e2c\u5404\u7a2e\u9776\u6a19\uff0c\u4f8b\u5982\u7d30\u80de\u56e0\u5b50(cytokines)\u3001\u751f\u9577\u56e0\u5b50(growth factors)\u3001\u8da8\u5316\u56e0\u5b50(chemokines)\u3001\u6fc0\u7d20(hormones)\u3001\u75c5\u6bd2\u6297\u539f(viral antigens)\u548c\u91cd\u7d44\u86cb\u767d(recombinant proteins)\u3002<\/p>\n

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD\/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species Reactivity<\/strong><\/td>\nPig (Sus scrofa; Porcine)<\/td>\n<\/tr>\n
UniProt<\/strong><\/td>\n<\/td>\n<\/tr>\n
Abbreviation<\/strong><\/td>\nToxoplasmosis IgG<\/td>\n<\/tr>\n
Alternative Names<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Range<\/strong><\/td>\nRequest Information<\/a><\/td>\n<\/tr>\n
Sensitivity<\/strong><\/td>\nRequest Information<\/a><\/td>\n<\/tr>\n
Sample Type<\/strong><\/td>\nSerum, Plasma, Other biological fluids<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nCompetitive ELISA<\/td>\n<\/tr>\n
Analysis Method<\/strong><\/td>\nQualitative<\/td>\n<\/tr>\n
Assay Duration<\/strong><\/td>\n1-3h<\/td>\n<\/tr>\n
Sample Volume<\/strong><\/td>\n1-200 \u03bcL<\/td>\n<\/tr>\n
Detection Wavelengt<\/strong><\/td>\n450 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5) <\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Assay plate (96 Wells)<\/td>\n1<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n
Standard (lyophilized)<\/td>\n2<\/td>\nSample Diluent<\/td>\n1\u00a0x 20 mL<\/td>\n<\/tr>\n
Biotin-Conjugate (concentrate\u00a0100 x)<\/td>\n1 x\u00a0120\u00a0\u03bcL<\/td>\nBiotin-Conjugate Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Streptavidin-HRP \u00a0(concentrate\u00a0100 x)<\/td>\n1 x 120\u00a0\u03bcL<\/td>\nStreptavidin-HRP Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Wash Buffer (concentrate\u00a025\u00a0x)<\/td>\n1 x 20\u00a0mL<\/td>\nSubstrate Solution<\/td>\n1 x 12\u00a0mL<\/td>\n<\/tr>\n
Stop Solution<\/td>\n1 x 10\u00a0mL<\/td>\nAdhesive Films<\/td>\n4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Matrices listed below were spiked with certain level of recombinant Pig Toxoplasmosis IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Pig Toxoplasmosis IgG in samples.<\/p>\n\n\n\n\n\n\n
Sample Type<\/td>\nNumber<\/td>\nRecovery range (%)<\/td>\nAverage(%)<\/td>\n<\/tr>\n
Serum<\/td>\n10<\/td>\n90-101<\/td>\n96<\/td>\n<\/tr>\n
EDTA plasma<\/td>\n10<\/td>\n89-97<\/td>\n93<\/td>\n<\/tr>\n
Heparin\u00a0plasma<\/td>\n10<\/td>\n91-99<\/td>\n95<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6a23\u54c1\u63a1\u96c6\u548c\u5132\u5b58<\/strong><\/p>\n

\u00a0<\/strong>\u8840\u6e05\uff1a\u4f7f\u7528\u8840\u6e05\u5206\u96e2\u7ba1 (SST)\uff0c\u8b93\u6a23\u54c1\u5728\u5ba4\u6eab\u4e0b\u51dd\u7d50 2 \u5c0f\u6642\u6216\u5728 2 – 8\u00b0C \u4e0b\u904e\u591c\uff0c\u7136\u5f8c\u4ee5 1000 \u00d7 g \u96e2\u5fc3 15 \u5206\u9418\u3002\u7acb\u5373\u53d6\u51fa\u8840\u6e05\u4e26\u9032\u884c\u6aa2\u6e2c\uff0c\u6216\u5c07\u6a23\u54c1\u7b49\u5206\u4e26\u5132\u5b58\u5728 \u2264 -20\u00b0C\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002\u8840\u6f3f\uff1a\u4f7f\u7528 EDTA \u6216\u809d\u7d20\u4f5c\u70ba\u6297\u51dd\u5291\u6536\u96c6\u8840\u6f3f\u3002\u6536\u96c6\u5f8c 30 \u5206\u9418\u5167\uff0c\u5728 2 – 8\u00b0C \u4e0b\u4ee5 1000 \u00d7 g \u96e2\u5fc3 15 \u5206\u9418\u3002\u7acb\u5373\u6e2c\u5b9a\u6216\u5206\u88dd\u6a23\u54c1\u4e26\u5728 \u2264 -20\u00b0C \u4e0b\u5132\u5b58\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002\u5176\u4ed6\u751f\u7269\u9ad4\u6db2\uff1a\u4ee5 1000 \u00d7 g \u96e2\u5fc3\u6a23\u54c1 20 \u5206\u9418\u3002\u7acb\u5373\u53bb\u9664\u5fae\u7c92\u4e26\u9032\u884c\u5206\u6790\uff0c\u6216\u5c07\u6a23\u54c1\u5206\u88dd\u5728 -20\u00b0C \u6216 -80\u00b0C \u4e0b\u5132\u5b58\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Code<\/strong><\/td>\nProduct Name<\/strong><\/td>\nSpec<\/strong><\/td>\nUsage<\/strong><\/td>\n<\/tr>\n
AE64456PI<\/td>\nClassical Swine Fever Virus Anitibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62688PI<\/td>\nPig Reproductive and respiratory syndrome virus (PRRSV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64454PI<\/td>\nPig Foot and Mouth Disease Virus O type Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64455PI<\/td>\nPig Foot and Mouth Disease Virus 3ABC Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE63272PI<\/td>\nPig Pseudorabies virus IgG antibody (PRV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64453PI<\/td>\nPig Pseudorabies gE virus IgG antibody (PRV-gE-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62689PI<\/td>\nPig Japanese Encephalitis IgG Antibody (JE-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64457PI<\/td>\nPig Parvovirus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64452PI<\/td>\nPig Circovirus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64451PI<\/td>\nPig Toxoplasmosis Virus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64450GE<\/td>\nAvian Influenza Virus Antibody (AIV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64449DU<\/td>\nDuck Avian Influenza Virus Antibody (AIV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62246CH<\/td>\nChicken Newcastle disease virus IgG antibody (NDV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62916CH<\/td>\nChicken Infectious bronchitis antibody (IB-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62955DO<\/td>\nCanine Rabies virus antibody (RV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62228BO<\/td>\nBovine Antibody against Foot and Mouth Disease IgM (FMDV-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62251GO<\/td>\nGoat Antibody against Foot and Mouth Disease IgM (FMDV-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62406HU<\/td>\nHuman Anti-toxoplasma IgG antibody (TOX-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62407HU<\/td>\nHuman Anti-toxoplasma IgM antibody (TOX-Ab-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62317HU<\/td>\nHuman Anti-hepatitis A virus antibody IgG (HAV-Ab-IgG) ELISA Kit <\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE29944RA<\/td>\nRat Mycoplasma Pulmonis\/M Arthriditis IgG antibody (MP-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE29993MO<\/td>\nMouse Mycoplasma Pulmonis\/M Arthriditis IgG antibody (MP-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n<\/td>\n<\/tr>\n
AE62689PI<\/a><\/td>\n\u8c6c\u65e5\u672c\u8166\u708e<\/strong>\u00a0IgG\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0(JE-Ab-IgG) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64456PI<\/a><\/td>\n\u8c6c\u7d93\u5178\u8c6c\u761f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(CSFV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64454PI<\/a><\/td>\n\u8c6c\u53e3\u8e44\u75ab\u75c5\u6bd2<\/strong>O<\/strong>\u578b\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64505PI<\/a><\/td>\n\u8c6c\u7d93\u5178\u8c6c\u761f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(CSFV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE63272PI<\/a><\/td>\n\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2<\/strong>\u00a0IgG\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0(PRV-Ab-IgG) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64453PI<\/a><\/td>\n\u8c6c\u507d\u72c2\u72ac\u75c5gE\u75c5\u6bd2IgG\u6297\u9ad4\uff08PRV-gE-Ab-IgG\uff09ELISA\u8a66\u5291\u76d2<\/a><\/strong><\/td>\n<\/tr>\n
AE64457PI<\/a><\/td>\n\u8c6c\u7d30\u5c0f\u75c5\u6bd2\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64452PI<\/a><\/td>\n\u8c6c\u5713\u74b0\u75c5\u6bd2\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64451PI<\/a><\/td>\n\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5<\/strong>\u00a0(IgG)\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64450GE<\/a><\/td>\n\u79bd\u6d41\u611f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(AIV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64449DU<\/a><\/td>\n\u9d28\u79bd\u6d41\u611f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(AIV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong>\u00a0<\/a><\/td>\n<\/tr>\n
\u00a0AE62916CH<\/a><\/td>\n\u96de\u50b3\u67d3\u6027\u652f\u6c23\u7ba1\u708e\u6297\u9ad4<\/strong>\u00a0(IB-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong>\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5 (IgG) \u6297\u9ad4 ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE64451PI Pig Anti-toxopl […]<\/p>\n","protected":false},"author":3,"featured_media":33544,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[4881],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33614"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33614"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33614\/revisions"}],"predecessor-version":[{"id":33971,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33614\/revisions\/33971"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33544"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33614"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33614"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}