{"id":33601,"date":"2022-08-03T14:30:36","date_gmt":"2022-08-03T06:30:36","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33601"},"modified":"2022-09-02T10:25:29","modified_gmt":"2022-09-02T02:25:29","slug":"%e8%b1%ac%e5%81%bd%e7%8b%82%e7%8a%ac%e7%97%85%e7%97%85%e6%af%92-igg-%e6%8a%97%e9%ab%94-prv-ab-igg-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fae63272pi","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33601","title":{"rendered":"\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2 IgG \u6297\u9ad4 (PRV-Ab-IgG) ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE63272PI"},"content":{"rendered":"

\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2<\/strong> IgG <\/strong>\u6297\u9ad4<\/strong> (PRV-Ab-IgG) ELISA <\/strong>\u8a66\u5291\u76d2<\/strong> | <\/strong>\u8ca8\u865f<\/strong>AE63272PI<\/a><\/span><\/p>\n

Pig Pseudorabies virus IgG antibody (PRV-Ab-IgG) ELISA Kit<\/p>\n

\"ABEBIO<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2<\/strong> IgG <\/strong>\u6297\u9ad4<\/strong> (PRV-Ab-IgG) ELISA <\/strong>\u8a66\u5291\u76d2<\/strong> \u00a0<\/u>Competitive Inhibition |\u8ca8\u865fAE63272PI\u3002ELISA\u539f\u7406\u70ba\u5229\u7528\u6297\u9ad4\u8207\u6297\u539f\u4e4b\u9593\u7684\u9ad8\u5ea6\u5c08\u4e00\u6027\uff0c\u505a\u70ba\u5b9a\u91cf\u6a19\u8a8c\u7684\u65b9\u6cd5\uff1b\u7121\u8ad6\u6e2c\u91cf\u6297\u539f\u6216\u6e2c\u91cf\u6297\u9ad4\u90fd\u6709\u5f88\u9ad8\u7684\u9748\u654f\u5ea6\u8ddf\u5c08\u4e00\u6027\u3002Abebio \u662f\u4e00\u5bb6\u9818\u5148\u7684\u751f\u7269\u6280\u8853\u516c\u53f8\uff0c\u5c08\u9580\u751f\u7522\u9ad8\u8cea\u91cf\u7684\u751f\u7269\u7522\u54c1\uff0c\u5982\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u548c ELISA \u8a66\u5291\u76d2\u3002 Abebio \u751f\u7522\u6578\u4ee5\u5343\u8a08\u7684 ELISA \u8a66\u5291\u76d2\uff0c\u7528\u65bc\u6aa2\u6e2c\u5404\u7a2e\u9776\u6a19\uff0c\u4f8b\u5982\u7d30\u80de\u56e0\u5b50(cytokines)\u3001\u751f\u9577\u56e0\u5b50(growth factors)\u3001\u8da8\u5316\u56e0\u5b50(chemokines)\u3001\u6fc0\u7d20(hormones)\u3001\u75c5\u6bd2\u6297\u539f(viral antigens)\u548c\u91cd\u7d44\u86cb\u767d(recombinant proteins)\u3002<\/p>\n

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD\/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species Reactivity<\/strong><\/td>\nPig (Sus scrofa; Porcine)<\/td>\n<\/tr>\n
UniProt<\/strong><\/td>\n<\/td>\n<\/tr>\n
Abbreviation<\/strong><\/td>\nPRV-Ab-IgG<\/td>\n<\/tr>\n
Alternative Names<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Range<\/strong><\/td>\nRequest Information<\/a><\/td>\n<\/tr>\n
Sensitivity<\/strong><\/td>\nRequest Information<\/a><\/td>\n<\/tr>\n
Sample Type<\/strong><\/td>\nSerum, Plasma, Other biological fluids<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nSandwich<\/td>\n<\/tr>\n
Analysis Method<\/strong><\/td>\nQualitative<\/td>\n<\/tr>\n
Assay Duration<\/strong><\/td>\n1-4.5h<\/td>\n<\/tr>\n
Sample Volume<\/strong><\/td>\n1-200 \u03bcL<\/td>\n<\/tr>\n
Detection Wavelengt<\/strong><\/td>\n450 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

This assay has high sensitivity and excellent specificity for detection of Pig PRV-Ab-IgG. No significant cross-reactivity or interference between Pig PRV-Ab-IgG and analogues was observed.<\/p>\n

\u6aa2\u6e2c\u8a66\u5291\u76d2 (\u9176\u806f\u514d\u75ab\u5438\u9644\u8a66\u9a57\u6cd5)<\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Assay plate (96 Wells)<\/td>\n1<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n
Standard (lyophilized)<\/td>\n2<\/td>\nSample Diluent<\/td>\n1\u00a0x 20 mL<\/td>\n<\/tr>\n
Biotin-Conjugate (concentrate\u00a0100 x)<\/td>\n1 x\u00a0120\u00a0\u03bcL<\/td>\nBiotin-Conjugate Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Streptavidin-HRP \u00a0(concentrate\u00a0100 x)<\/td>\n1 x 120\u00a0\u03bcL<\/td>\nStreptavidin-HRP Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Wash Buffer (concentrate\u00a025\u00a0x)<\/td>\n1 x 20\u00a0mL<\/td>\nSubstrate Solution<\/td>\n1 x 12\u00a0mL<\/td>\n<\/tr>\n
Stop Solution<\/td>\n1 x 10\u00a0mL<\/td>\nAdhesive Films<\/td>\n4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong>The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig PRV-Ab-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.<\/p>\n\n\n\n\n\n\n
Sample Type<\/td>\n1\uff1a2<\/td>\n1\uff1a4<\/td>\n1\uff1a8<\/td>\n1\uff1a16<\/td>\n<\/tr>\n
Serum<\/td>\n78-89%<\/td>\n81-99%<\/td>\n92-103%<\/td>\n95-105%<\/td>\n<\/tr>\n
EDTA plasma<\/td>\n91-101%<\/td>\n90-98%<\/td>\n93-101%<\/td>\n91-98%<\/td>\n<\/tr>\n
Heparin\u00a0plasma<\/td>\n92-103%<\/td>\n93-102%<\/td>\n92-99%<\/td>\n91-101%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6a23\u54c1\u63a1\u96c6\u548c\u5132\u5b58<\/strong><\/p>\n

\u00a0<\/strong>\u8840\u6e05\uff1a\u4f7f\u7528\u8840\u6e05\u5206\u96e2\u7ba1 (SST)\uff0c\u8b93\u6a23\u54c1\u5728\u5ba4\u6eab\u4e0b\u51dd\u7d50 2 \u5c0f\u6642\u6216\u5728 2 – 8\u00b0C \u4e0b\u904e\u591c\uff0c\u7136\u5f8c\u4ee5 1000 \u00d7 g \u96e2\u5fc3 15 \u5206\u9418\u3002\u7acb\u5373\u53d6\u51fa\u8840\u6e05\u4e26\u9032\u884c\u6aa2\u6e2c\uff0c\u6216\u5c07\u6a23\u54c1\u7b49\u5206\u4e26\u5132\u5b58\u5728 \u2264 -20\u00b0C\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002\u8840\u6f3f\uff1a\u4f7f\u7528 EDTA \u6216\u809d\u7d20\u4f5c\u70ba\u6297\u51dd\u5291\u6536\u96c6\u8840\u6f3f\u3002\u6536\u96c6\u5f8c 30 \u5206\u9418\u5167\uff0c\u5728 2 – 8\u00b0C \u4e0b\u4ee5 1000 \u00d7 g \u96e2\u5fc3 15 \u5206\u9418\u3002\u7acb\u5373\u6e2c\u5b9a\u6216\u5206\u88dd\u6a23\u54c1\u4e26\u5728 \u2264 -20\u00b0C \u4e0b\u5132\u5b58\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002\u5176\u4ed6\u751f\u7269\u9ad4\u6db2\uff1a\u4ee5 1000 \u00d7 g \u96e2\u5fc3\u6a23\u54c1 20 \u5206\u9418\u3002\u7acb\u5373\u53bb\u9664\u5fae\u7c92\u4e26\u9032\u884c\u5206\u6790\uff0c\u6216\u5c07\u6a23\u54c1\u5206\u88dd\u5728 -20\u00b0C \u6216 -80\u00b0C \u4e0b\u5132\u5b58\u3002\u907f\u514d\u91cd\u8907\u51cd\u878d\u5faa\u74b0\u3002<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Code<\/strong><\/td>\nProduct Name<\/strong><\/td>\nSpec<\/strong><\/td>\nUsage<\/strong><\/td>\n<\/tr>\n
AE64456PI<\/td>\nClassical Swine Fever Virus Anitibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62688PI<\/td>\nPig Reproductive and respiratory syndrome virus (PRRSV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64454PI<\/td>\nPig Foot and Mouth Disease Virus O type Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64455PI<\/td>\nPig Foot and Mouth Disease Virus 3ABC Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE63272PI<\/td>\nPig Pseudorabies virus IgG antibody (PRV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64453PI<\/td>\nPig Pseudorabies gE virus IgG antibody (PRV-gE-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62689PI<\/td>\nPig Japanese Encephalitis IgG Antibody (JE-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64457PI<\/td>\nPig Parvovirus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64452PI<\/td>\nPig Circovirus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64451PI<\/td>\nPig Toxoplasmosis Virus Antibody ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64450GE<\/td>\nAvian Influenza Virus Antibody (AIV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE64449DU<\/td>\nDuck Avian Influenza Virus Antibody (AIV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62246CH<\/td>\nChicken Newcastle disease virus IgG antibody (NDV-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62916CH<\/td>\nChicken Infectious bronchitis antibody (IB-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62955DO<\/td>\nCanine Rabies virus antibody (RV-Ab) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62228BO<\/td>\nBovine Antibody against Foot and Mouth Disease IgM (FMDV-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62251GO<\/td>\nGoat Antibody against Foot and Mouth Disease IgM (FMDV-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62406HU<\/td>\nHuman Anti-toxoplasma IgG antibody (TOX-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62407HU<\/td>\nHuman Anti-toxoplasma IgM antibody (TOX-Ab-IgM) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE62317HU<\/td>\nHuman Anti-hepatitis A virus antibody IgG (HAV-Ab-IgG) ELISA Kit <\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE29944RA<\/td>\nRat Mycoplasma Pulmonis\/M Arthriditis IgG antibody (MP-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n
AE29993MO<\/td>\nMouse Mycoplasma Pulmonis\/M Arthriditis IgG antibody (MP-Ab-IgG) ELISA Kit<\/a><\/u><\/td>\n96 Tests<\/td>\nSerum\u3001plasma<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n<\/td>\n<\/tr>\n
AE62689PI<\/a><\/td>\n\u8c6c\u65e5\u672c\u8166\u708e<\/strong>\u00a0IgG\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0(JE-Ab-IgG) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64456PI<\/a><\/td>\n\u8c6c\u7d93\u5178\u8c6c\u761f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(CSFV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64454PI<\/a><\/td>\n\u8c6c\u53e3\u8e44\u75ab\u75c5\u6bd2<\/strong>O<\/strong>\u578b\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64505PI<\/a><\/td>\n\u8c6c\u7d93\u5178\u8c6c\u761f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(CSFV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE63272PI<\/a><\/td>\n\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2<\/strong>\u00a0IgG\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0(PRV-Ab-IgG) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64453PI<\/a><\/td>\n\u8c6c\u507d\u72c2\u72ac\u75c5gE\u75c5\u6bd2IgG\u6297\u9ad4\uff08PRV-gE-Ab-IgG\uff09ELISA\u8a66\u5291\u76d2<\/a><\/strong><\/td>\n<\/tr>\n
AE64457PI<\/a><\/td>\n\u8c6c\u7d30\u5c0f\u75c5\u6bd2\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64452PI<\/a><\/td>\n\u8c6c\u5713\u74b0\u75c5\u6bd2\u6297\u9ad4<\/strong>ELISA<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64451PI<\/a><\/td>\n\u8c6c\u6297\u5f13\u5f62\u9ad4\u75c5<\/strong>\u00a0(IgG)\u00a0<\/strong>\u6297\u9ad4<\/strong>\u00a0ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64450GE<\/a><\/td>\n\u79bd\u6d41\u611f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(AIV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong><\/a><\/td>\n<\/tr>\n
AE64449DU<\/a><\/td>\n\u9d28\u79bd\u6d41\u611f\u75c5\u6bd2\u6297\u9ad4<\/strong>\u00a0(AIV-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong>\u00a0<\/a><\/td>\n<\/tr>\n
\u00a0AE62916CH<\/a><\/td>\n\u96de\u50b3\u67d3\u6027\u652f\u6c23\u7ba1\u708e\u6297\u9ad4<\/strong>\u00a0(IB-Ab) ELISA\u00a0<\/strong>\u8a66\u5291\u76d2<\/strong>\u00a0<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u8c6c\u507d\u72c2\u72ac\u75c5\u75c5\u6bd2 IgG \u6297\u9ad4 (PRV-Ab-IgG) ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE63272PI Pig […]<\/p>\n","protected":false},"author":3,"featured_media":33544,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452,462],"tags":[4877],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33601"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33601"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33601\/revisions"}],"predecessor-version":[{"id":33967,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33601\/revisions\/33967"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33544"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33601"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33601"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33601"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}