{"id":33567,"date":"2022-08-02T15:12:03","date_gmt":"2022-08-02T07:12:03","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33567"},"modified":"2024-07-26T10:41:40","modified_gmt":"2024-07-26T02:41:40","slug":"onestep-blocker%e5%b0%81%e9%96%89%e6%8a%97%e9%ab%94%e5%a2%9e%e5%bc%b7%e6%ba%b6%e6%b6%b2-bio-helix%e8%b2%a8%e8%99%9f-bs001-b500ml","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33567","title":{"rendered":"OneStep Blocker\u5c01\u9589&#038;\u6297\u9ad4\u589e\u5f37\u6eb6\u6db2 | BIO-HELIX\u8ca8\u865f BS001-B500ML"},"content":{"rendered":"<p><span style=\"color: #33cccc;\"><strong><span style=\"font-size: 12pt;\"><a style=\"color: #33cccc;\" href=\"http:\/\/biopioneer.com.tw\/?p=33567\">OneStep Blocker\u5c01\u9589&amp;\u6297\u9ad4\u589e\u5f37\u6eb6\u6db2 | BIO-HELIX\u8ca8\u865f BS001-B500ML<\/a><\/span><\/strong><\/span><\/p>\n<p>OneStep Blocker \u7de9\u885d\u6db2\u4e00\u500b\u6b65\u9a5f\u540c\u6642\u5b8c\u6210\u5c01\u9589(blocking)\u3001\u4e00\u6297\u548c\u4e8c\u6297\u96dc\u4ea4(hybridization)\u7b49\u4e09\u500b\u5be6\u9a57\u6b65\u9a5f\uff0cOneStep Blocker \u540c\u6642\u4e5f\u80fd\u589e\u5f37 HRP\uff08horseradish peroxidase\uff09\u6216 AP\uff08alkaline phosphatase, \u9e7c\u6027\u78f7\u9178\u9176\uff09\u5e95\u7269\u7522\u751f\u7684signal\u3002\u5728\u897f\u65b9\u5206\u6790(Western analysis)\u4e2d\u65e2\u662f\u963b\u65b7\u5291(blocker)\u53c8\u662f\u589e\u5f37\u5291(enhancer)\u3002\u4e0d\u542b\u86cb\u767d\u8cea\u914d\u65b9(Protein free)\u5927\u5e45\u6e1b\u5c11 ECL \u6aa2\u6e2c\u4e2d\u5e38\u898b\u7684\u975e\u7279\u7570\u6027\u4fe1\u865f\uff0c\u964d\u4f4e\u80cc\u666f\u503c\u3002\u9069\u7528\u65bc\u6240\u6709PVDF &amp; NC membrane\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.bio-helix.com\/assets\/bs5-b76690e64b6ed9045cb16069f8c091cff6dcf61aa580fe9bc184f02474bc53e5.png\" alt=\"Bs5\" width=\"122\" height=\"30\" \/><\/p>\n<p><img decoding=\"async\" src=\"http:\/\/www.bio-helix.com\/uploads\/ckeditor\/pictures\/96\/content_BS001-B500ML_01-01.jpg\" alt=\"\" \/><\/p>\n<p>Mix OneStep Blocker, 1\u00b0Ab and 2\u00b0 Ab with membrane together, that&#8217;s all.<\/p>\n<p><strong>OneStep Blocker\u00a0<\/strong>is a blocking solution for Western blot analysis. This\u00a0<strong>OneStep Blocker<\/strong>\u00a0buffer not only provides blocking, primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the 3-in-1 step procedure,\u00a0<strong>OneStep Blocker<\/strong>\u00a0is a time and labor economic solution for the time consuming and laborious Western procedure.<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/TOP-SALES-150x150.png\" alt=\"\" width=\"119\" height=\"107\" \/><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.bio-helix.com\/uploads\/product\/photo\/230\/BS001-B500ML.png\" alt=\"Bs001 b500ml\" width=\"273\" height=\"305\" \/><\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/08\/OneStep-Blocker\u5c01\u9589\u6297\u9ad4\u589e\u5f37\u6eb6\u6db2-BIO-HELIX\u8ca8\u865f-BS001-B500ML.pdf\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E5%BB%A0%E6%93%8D%E4%BD%9C%E4%B8%8B%E8%BC%89-300x75.png\" alt=\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\" width=\"143\" height=\"37\" \/><\/a><\/p>\n<p><strong>Features of OneStep Blocker &#8211; Western blocking solution and a signal enhancer\u00a0(Protein free)<\/strong><\/p>\n<ul>\n<li><strong>3 steps in one<\/strong>: Block the membrane and dilute\u00a01o\u00a0\u00a0Ab &amp; 2o\u00a0\u00a0Ab in one step.<\/li>\n<li><strong>Enhance antibody signal:<\/strong>\u00a0It shows a two- to five-fold increase in signal intensity for most protein targets,\u00a0enabling much less protein to be detected with the same substrate and method.<\/li>\n<li><strong>Enhance time-saving:<\/strong>\u00a0It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.<\/li>\n<li><strong>Universal antibody diluent<\/strong>: Ready-to-use dilution buffer for most of\u00a01o\u00a0\u00a0Ab &amp; 2o\u00a0\u00a0Ab.<\/li>\n<li><strong>No blocking step needed:<\/strong>\u00a0Just immerse the membrane in the OneStep Blocker solution with your antibodies. That&#8217;s all.<\/li>\n<li><strong>Effective with any ECL:\u00a0<\/strong>After the antibody detection process,\u00a0the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.<\/li>\n<li><strong>Less hands-on steps:<\/strong>\u00a0No 3 wash steps are required, meaning no need to transfer the membrane in&amp;out of the container.<\/li>\n<li><strong>Compatible with PVDF &amp; NC membrane:<\/strong>\u00a0Regardless of the pore size, the OneStep Blocker minimizes the background from non-specific protein binding by antibodies.<\/li>\n<li><strong>Improve protein detection:<\/strong>\u00a0Improve the binding process of\u00a0target proteins, so that specific antibodies can bind more effectively.<\/li>\n<li><strong>Protein free:\u00a0<\/strong>Reduces overall background and minimizes non-specific signals often seen with ECL detection.<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.bio-helix.com\/uploads\/ckeditor\/pictures\/98\/content_1020_%E6%AD%A5%E9%A9%9F_%E7%9B%B4%E5%BC%8F_OneStep_Blocker_II-05.png\" alt=\"\" width=\"598\" height=\"607\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.bio-helix.com\/uploads\/ckeditor\/pictures\/97\/content_BS001-B500ML_01-02.jpg\" alt=\"\" width=\"440\" height=\"342\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/www.bio-helix.com\/uploads\/ckeditor\/pictures\/112\/content_%E7%B6%B2%E9%A0%81%E7%94%A8_%E9%98%B2%E5%91%86%E5%8D%A1-01-.jpg\" alt=\"\" width=\"735\" height=\"1158\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E4%BD%BF%E7%94%A8%E6%96%B9%E6%B3%95A-300x92.png\" alt=\"\u00e4\u00bd\u00bf\u00e7\u0094\u00a8\u00e6\u0096\u00b9\u00e6\u00b3\u0095A\" width=\"114\" height=\"37\" \/><\/p>\n<ol>\n<li>Western blot \u8f49\u79fb\u5f8c\uff0c\u5c07 PVDF \u6216 NC \u819c\u6d78\u5165 PBST \u7de9\u885d\u6db2\u4e2d 5 \u5206\u9418\u3002<\/li>\n<li>\u7528\u9069\u91cf\u7684 OneStep Blocker \u7a00\u91cb\u4e00\u6297\u548c\u4e8c\u6297\u3002<\/li>\n<\/ol>\n<p>&#8212;&#8212;2.1 \u4f8b\u5982\uff0c\u7576\u4e00\u6297\u548c\u4e8c\u6297\u7684\u7a00\u91cb\u500d\u6578\u70ba 1\uff1a10,000 \u6642\uff0c\u5c07 2\u03bcl \u4e00\u6297\u52a0\u5165 10ml OneStep Blocker\uff08\u7b2c 1 \u7ba1\uff09\u4e2d\uff0c\u7136\u5f8c\u5c07 2\u03bcl \u4e8c\u6297\u52a0\u5165\u53e6\u5916 10ml OneStep\u963b\u6eef\u5291\uff08\u7b2c<\/p>\n<p>2\u00a0 \u7ba1\uff09\u3002<\/p>\n<p>&#8212;&#8212;2.2 \u901a\u904e\u4f86\u56deinverting\u5c07\u6bcf\u500b\u7ba1\u5167\u7684\u6297\u9ad4-OneStep Blocker \u6eb6\u6db2\u5fb9\u5e95\u6df7\u5408\u3002<\/p>\n<p>&#8212;&#8212;2.3 \u5148\u5c07\u4e00\u6297-OneStep Blocker \u6eb6\u6db2\u5012\u5165\u6e96\u5099\u597d\u7684\u5bb9\u5668\u4e2d\uff0c\u7136\u5f8c\u5c07\u4e8c\u6297-OneStep Blocker \u6eb6\u6db2\u52a0\u5165\u540c\u4e00\u500b\u5bb9\u5668\u4e2d\u3002<\/p>\n<ol start=\"3\">\n<li>\u7acb\u5373\u5c07\u819c\u5728\u6297\u9ad4-OneStep Blocker \u6eb6\u6db2\u4e2d\u5ba4\u6eab\u5b75\u80b2 1-2 \u5c0f\u6642\uff0c\u540c\u6642\u8f15\u8f15\u652a\u62cc\u3002\u8acb\u6ce8\u610f\uff0c\u4e00\u6297\u548c\u4e8c\u6297\u6df7\u5408\u5f8c\uff0c\u819c\u9700\u8981\u5728 10 \u5206\u9418\u5167\u7acb\u5373\u6d78\u5165\u6df7\u5408\u7269\u4e2d\uff0c\u4ee5\u7372\u5f97\u6700\u4f73\u6027\u80fd\u3002<\/li>\n<li>\u7528 PBST\/TBST \u632f\u76ea\u6d17\u6ecc\u819c 3 \u6b21\u3002<\/li>\n<li>\u6392\u51fa\u904e\u591a\u7684\u6d17\u6ecc\u7de9\u885d\u6db2\u4e26\u7acb\u5373\u4f7f\u7528 ECL \u6216\u6bd4\u8272\u7cfb\u7d71\u9032\u884c\u986f\u50cf\u3002<\/li>\n<\/ol>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E4%BD%BF%E7%94%A8%E6%96%B9%E6%B3%95B-300x92.png\" alt=\"\u00e4\u00bd\u00bf\u00e7\u0094\u00a8\u00e6\u0096\u00b9\u00e6\u00b3\u0095B\" width=\"114\" height=\"37\" \/><\/p>\n<p>\u6ce8\u610f\u4e8b\u9805<\/p>\n<p>\u4e00\u6297\u548c\u4e8c\u6297\u6df7\u5408\u5f8c\uff0c\u9700\u8981\u5728 10 \u5206\u9418\u5167\u7acb\u5373\u5c07\u819c\u6d78\u5165\u6df7\u5408\u7269\u4e2d\uff0c\u4ee5\u7372\u5f97\u6700\u4f73\u6027\u80fd\u3002\u4e8c\u6297\u7684\u7a00\u91cb\u5ea6\u61c9\u81f3\u5c11\u70ba 1:10,000 \u6216\u66f4\u9ad8\u3002\u4f7f\u7528\u9ad8\u6fc3\u5ea6\u4e8c\u6297\u6642\u6703\u89c0\u5bdf\u5230\u66f4\u9ad8\u6c34\u5e73\u7684\u80cc\u666f\u566a\u97f3\u3002\u4e0d\u8981\u5728 OneStep Blocker \u4e2d\u5b75\u80b2\u819c\u8d85\u904e 4 \u5c0f\u6642\uff0c\u4ee5\u907f\u514d\u9ad8\u80cc\u666f\u3002\u5c24\u5176\u4e0d\u63a8\u85a6\u904e\u591c\u5b75\u80b2(O\/N)\u3002OneStep Blocker \u6eb6\u6db2\u4e2d\u6df7\u5408\u7684\u4e00\u6297\/\u4e8c\u6297\u53ef\u5728 3 \u5929\u5167\u91cd\u8907\u4f7f\u7528\u3002\u96a8\u8457\u5b58\u5132\u6642\u9593\u6216\u91cd\u8907\u6027\u7684\u589e\u52a0\uff0c\u589e\u5f37\u6548\u679c\u53ef\u80fd\u6703\u9010\u6f38\u6e1b\u5f31\u3002\u5c07\u6df7\u5408\u6eb6\u6db2\u51b7\u85cf\u3002\u5c0d\u65bc\u95dc\u9375\u5be6\u9a57\u6216\u5f37\u4fe1\u865f\uff0c\u9700\u8981\u65b0\u9bae\u88fd\u5099\u6297\u9ad4 OneStep Blocker \u6eb6\u6db2\u3002\u6297\u9ad4\u6fc3\u5ea6\u904e\u9ad8\u6216\u5b75\u80b2\u6642\u9593\u904e\u9577\uff0c\u6703\u5f15\u8d77\u9ad8\u80cc\u666f\u3002\u7576\u51fa\u73fe\u904e\u591a\u80cc\u666f\u6642\uff0c\u8acb\u5617\u8a66\u4ee5\u4e0b\u65b9\u6cd5\uff1a<\/p>\n<p>(a) \u964d\u4f4e\/\u512a\u5316\u4e00\u6297\u548c\/\u6216\u4e8c\u6297\u6fc3\u5ea6\u3002<\/p>\n<p>(b) \u4f7f\u7528\u6591\u9ede\u5370\u8de1\u6e2c\u8a66\u512a\u5316\u6297\u9ad4\u6fc3\u5ea6\u3002<\/p>\n<p>(c) \u6e1b\u5c11\/\u512a\u5316\u5b75\u80b2\u6642\u9593\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>OneStep Blocker\u5c01\u9589&amp;\u6297\u9ad4\u589e\u5f37\u6eb6\u6db2 | BIO-HELIX\u8ca8\u865f BS001-B500ML [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":33568,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5372],"tags":[4870],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33567"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33567"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33567\/revisions"}],"predecessor-version":[{"id":49871,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33567\/revisions\/49871"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33568"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33567"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33567"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33567"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}