{"id":33561,"date":"2022-08-01T16:21:10","date_gmt":"2022-08-01T08:21:10","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33561"},"modified":"2022-08-02T15:13:11","modified_gmt":"2022-08-02T07:13:11","slug":"%e4%ba%ba%e9%80%8f%e6%98%8e%e8%b3%aa%e9%85%b8-ha-elisa-%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9fae40537hu","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33561","title":{"rendered":"\u4eba\u900f\u660e\u8cea\u9178 (HA) ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE40537HU"},"content":{"rendered":"

\u4eba\u900f\u660e\u8cea\u9178 (HA) ELISA \u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>AE40537HU<\/a><\/span><\/p>\n

Human Hyaluronic acid (HA) ELISA Kit<\/p>\n

\u8a72\u6e2c\u5b9a\u63a1\u7528\u96d9\u4f4d\u9ede\u593e\u5fc3(two-site sandwich ELISA) ELISA \u4f86\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684 \u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA)\u3002 \u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA)\u7279\u7570\u6027\u6297\u9ad4\u5df2\u9810\u5148\u5305\u88ab\u5728\u5fae\u5b54\u677f\u4e0a\u3002\u6a19\u6e96\u54c1\u548c\u6a23\u54c1\u88ab\u79fb\u6db2\u5230\u5b54\u4e2d\uff0c\u4efb\u4f55\u5b58\u5728\u7684 \u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA)\u90fd\u88ab\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u3002\u5728\u53bb\u9664\u4efb\u4f55\u672a\u7d50\u5408\u7684\u7269\u8cea\u5f8c\uff0c\u5c07\u4e00\u7a2e\u751f\u7269\u7d20\u5076\u806f(biotin-conjugated antibody)\u7684 HA \u7279\u7570\u6027\u6297\u9ad4\u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u6d17\u6ecc\u5f8c\uff0c\u5c07\u93c8\u9ef4\u89aa\u548c\u7d20\u7db4\u5408\u7684\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 (HRP) \u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u5728\u6d17\u6ecc\u4ee5\u53bb\u9664\u4efb\u4f55\u672a\u7d50\u5408\u7684\u6297\u751f\u7269\u7d20\u86cb\u767d\u9176\u8a66\u5291\u5f8c\uff0c\u5c07\u5e95\u7269\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u984f\u8272\u8207\u521d\u59cb\u6b65\u9a5f\u4e2d\u7d50\u5408\u7684\u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA) \u7684\u91cf\u6210\u6bd4\u4f8b\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u8a72\u65b9\u6cd5\u5c0d\u4eba\u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA)\u7684\u6aa2\u6e2c\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u548c\u512a\u7570\u7684\u7279\u7570\u6027\u3002\u672a\u89c0\u5bdf\u5230\u4eba \u900f\u660e\u8cea\u9178(<\/strong>Hyaluronic acid , HA)\u548c\u985e\u4f3c\u7269\u4e4b\u9593\u7684\u986f\u8457\u4ea4\u53c9\u53cd\u61c9(cross-reactivity)\u6216\u5e72\u64fe(interference)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species Reactivity<\/strong><\/td>\nHuman (Homo sapiens)<\/td>\n<\/tr>\n
UniProt<\/strong><\/td>\n<\/td>\n<\/tr>\n
Abbreviation<\/strong><\/td>\nHA<\/td>\n<\/tr>\n
Alternative Names<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Range<\/strong><\/td>\n1.56-100 ng\/mL<\/td>\n<\/tr>\n
Sensitivity<\/strong><\/td>\n0.78 ng\/mL<\/td>\n<\/tr>\n
Sample Type<\/strong><\/td>\nSerum, Plasma, Other biological fluids<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nSandwich<\/td>\n<\/tr>\n
Analysis Method<\/strong><\/td>\nQuantitive<\/td>\n<\/tr>\n
Assay Duration<\/strong><\/td>\n1-4.5h<\/td>\n<\/tr>\n
Sample Volume<\/strong><\/td>\n1-200 \u03bcL<\/td>\n<\/tr>\n
Detection Wavelengt<\/strong><\/td>\n450 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Assay plate (96 Wells)<\/td>\n1<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n
Standard (lyophilized)<\/td>\n2<\/td>\nSample Diluent<\/td>\n1\u00a0x 20 mL<\/td>\n<\/tr>\n
Biotin-Conjugate (concentrate\u00a0100 x)<\/td>\n1 x\u00a0120\u00a0\u03bcL<\/td>\nBiotin-Conjugate Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Streptavidin-HRP \u00a0(concentrate\u00a0100 x)<\/td>\n1 x 120\u00a0\u03bcL<\/td>\nStreptavidin-HRP Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Wash Buffer (concentrate\u00a025\u00a0x)<\/td>\n1 x 20\u00a0mL<\/td>\nSubstrate Solution<\/td>\n1 x 12\u00a0mL<\/td>\n<\/tr>\n
Stop Solution<\/td>\n1 x 10\u00a0mL<\/td>\nAdhesive Films<\/td>\n4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Matrices listed below were spiked with certain level of recombinant Human HA and the recovery rates were calculated by comparing the measured value to the expected amount of Human HA in samples.<\/p>\n\n\n\n\n\n\n
Sample Type<\/td>\nNumber<\/td>\nRecovery range (%)<\/td>\nAverage(%)<\/td>\n<\/tr>\n
Serum<\/td>\n10<\/td>\n90-101<\/td>\n96<\/td>\n<\/tr>\n
EDTA plasma<\/td>\n10<\/td>\n89-97<\/td>\n93<\/td>\n<\/tr>\n
Heparin\u00a0plasma<\/td>\n10<\/td>\n91-99<\/td>\n95<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD\/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%<\/p>\n\n\n\n\n\n\n
Sample Type<\/td>\n1\uff1a2<\/td>\n1\uff1a4<\/td>\n1\uff1a8<\/td>\n1\uff1a16<\/td>\n<\/tr>\n
Serum<\/td>\n78-89%<\/td>\n81-99%<\/td>\n92-103%<\/td>\n95-105%<\/td>\n<\/tr>\n
EDTA plasma<\/td>\n91-101%<\/td>\n90-98%<\/td>\n93-101%<\/td>\n91-98%<\/td>\n<\/tr>\n
Heparin\u00a0plasma<\/td>\n92-103%<\/td>\n93-102%<\/td>\n92-99%<\/td>\n91-101%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

\u4eba\u900f\u660e\u8cea\u9178 (HA) ELISA \u8a66\u5291\u76d2 | \u8ca8\u865fAE40537HU Human Hyaluronic aci […]<\/p>\n","protected":false},"author":3,"featured_media":33544,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[4868],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33561"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33561"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33561\/revisions"}],"predecessor-version":[{"id":33562,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33561\/revisions\/33562"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33544"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33561"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33561"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33561"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}