{"id":33546,"date":"2022-08-01T12:56:30","date_gmt":"2022-08-01T04:56:30","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33546"},"modified":"2022-08-02T15:12:49","modified_gmt":"2022-08-02T07:12:49","slug":"%e4%ba%ba%e6%96%b0%e5%9e%8b%e5%86%a0%e7%8b%80%e7%97%85%e6%af%92%e6%a0%b8%e8%9b%8b%e7%99%bdigm%e6%8a%97%e9%ab%94%ef%bc%882019-ncov-ab-igm%ef%bc%89%e6%aa%a2%e6%b8%ac-%e8%b2%a8%e8%99%9fae64578hu","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33546","title":{"rendered":"\u4eba\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\u6838\u86cb\u767dIgM\u6297\u9ad4\uff082019-nCoV-Ab-IgM\uff09\u6aa2\u6e2c | \u8ca8\u865fAE64578HU"},"content":{"rendered":"

\u4eba\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\u6838\u86cb\u767dIgM\u6297\u9ad4\uff082019-nCoV-Ab-IgM\uff09ELISA\u8a66\u5291\u76d2 <\/strong>| <\/strong>\u8ca8\u865f<\/strong>AE64578HU<\/a><\/span><\/p>\n

Human Novel Coronavirus Nucleoprotein IgM antibody (2019-nCoV-Ab-IgM) ELISA Kit
\n\"\"<\/p>\n

\u51a0\u72c0\u75c5\u6bd2(Coronaviruses)\u662f\u5177\u6709\u6b63\u7fa9 RNA \u57fa\u56e0\u7d44(positive-sense RNA genome)\u548c\u87ba\u65cb\u5c0d\u7a31\u6838\u8863\u6bbc(nucleocapsid)\u7684\u5305\u819c\u75c5\u6bd2(enveloped viruses)\u3002\u5728\u75c5\u6bd2\u611f\u67d3\u7684\u539f\u4ee3\u7d30\u80de(virus-infected primary cells)\u548c\u7528\u8868\u9054 N \u86cb\u767d(N protein)\u7684\u8cea\u7c92\u8f49\u67d3\u7684\u7d30\u80de(cells transfected with plasmids)\u4e2d\uff0c\u51a0\u72c0\u75c5\u6bd2\u6838\u86cb\u767d(Coronavirus nucleoproteins)\u5b9a\u4f4d\u65bc\u7d30\u80de\u8cea(cytoplasm)\u548c\u6838\u4ec1(nucleolus)\uff0c\u9019\u662f\u4e00\u7a2e\u4e9e\u6838\u7d50\u69cb(subnuclear structure)\u3002\u51a0\u72c0\u75c5\u6bd2 N \u86cb\u767d(Coronavirus N protein) \u662f\u51a0\u72c0\u75c5\u6bd2 RNA \u5408\u6210(coronavirus RNA synthesis)\u6240\u5fc5\u9700\u7684\uff0c\u5177\u6709\u53ef\u80fd\u53c3\u8207\u6a21\u677f\u8f49\u63db(template switch)\u7684 RNA \u4f34\u4fb6\u6d3b\u6027(RNA chaperone activity)\u3002\u6838\u8863\u6bbc\u86cb\u767d(Nucleocapsid protein)\u662f\u51a0\u72c0\u75c5\u6bd2(coronavirus)\u4e2d\u542b\u91cf\u6700\u8c50\u5bcc\u7684\u86cb\u767d\u8cea\u3002\u5728\u75c5\u6bd2\u7c92\u5b50\u7d44\u88dd(virion assembly)\u904e\u7a0b\u4e2d\uff0cN \u86cb\u767d(N protein)\u8207\u75c5\u6bd2 RNA (viral RNA)\u7d50\u5408\u4f75\u5c0e\u81f4\u5f62\u6210\u87ba\u65cb\u72c0\u6838\u8863\u6bbc(helical nucleocapsid)\u3002\u6838\u8863\u6bbc\u86cb\u767d(Nucleocapsid protein)\u662f\u4e00\u7a2e\u9ad8\u5ea6\u514d\u75ab\u539f\u6027\u7684\u78f7\u86cb\u767d(phosphoprotein)\uff0c\u4e5f\u8207\u75c5\u6bd2\u57fa\u56e0\u7d44\u8907\u88fd(genome replication)\u548c\u8abf\u7bc0\u7d30\u80de\u4fe1\u865f\u901a\u8def(cell signaling pathways)\u6709\u95dc\u3002\u7531\u65bcN\u86cb\u767d\u5e8f\u5217\u4fdd\u5b88(conservation)\uff0c\u514d\u75ab\u539f\u6027\u5f37\uff0c\u56e0\u6b64\u9078\u64c7\u51a0\u72c0\u75c5\u6bd2N\u86cb\u767d(N protein)\u4f5c\u70ba\u8a3a\u65b7\u5de5\u5177\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u8a72\u8a66\u5291\u76d2\u4e2d\u63d0\u4f9b\u7684\u5fae\u91cf\u6ef4\u5b9a\u677f\u5df2\u9810\u5857\u6709 2019-nCoV (N) \u86cb\u767d\u3002\u7136\u5f8c\u5c07\u6a23\u54c1\u6dfb\u52a0\u5230\u5e36\u6709Horseradish Peroxidase (HRP) \u7db4\u5408\u7684anti-human IgM antibody \u6297\u9ad4\u7684\u9069\u7576microtiter plate wells\u4e2d\u4e26\u5b75\u80b2\u3002\u7136\u5f8c\u5c07\u5e95\u7269\u6eb6\u6db2(substrate solutions)\u6dfb\u52a0\u5230\u6bcf\u500b\u5b54\u4e2d\u3002\u53ea\u6709\u90a3\u4e9b\u542b\u6709 2019-nCoV-Ab-IgM \u548c\u9176\u806fanti-human IgM antibody \u6297\u9ad4\u7684\u5b54\u624d\u6703\u51fa\u73fe\u984f\u8272\u8b8a\u5316\u3002\u901a\u904e\u6dfb\u52a0\u786b\u9178\u6eb6\u6db2(sulphuric acid solution)\u7d42\u6b62\u9176-\u5e95\u7269\u53cd\u61c9\uff0c\u4e26\u5728 450 nm \u00b1 2 nm \u6ce2\u9577\u4e0b\u7528\u5206\u5149\u5149\u5ea6\u8a08\u6e2c\u91cf\u984f\u8272\u8b8a\u5316\u3002\u8a72\u65b9\u6cd5\u5c0d\u6aa2\u6e2c\u4eba 2019-nCoV-Ab-IgM \u5177\u6709\u9ad8\u9748\u654f\u5ea6(sensitivity)\u548c\u51fa\u8272\u7684\u7279\u7570\u6027(specificity)\u3002\u672a\u89c0\u5bdf\u5230\u4eba 2019-nCoV-Ab-IgM \u8207\u985e\u4f3c\u7269\u4e4b\u9593\u5b58\u5728\u986f\u8457\u7684\u4ea4\u53c9\u53cd\u61c9(cross-reactivity)\u6216\u5e72\u64fe(interference)\u3002<\/p>\n

\"ABEBIO<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Species Reactivity<\/strong><\/td>\nHuman (Homo sapiens)<\/td>\n<\/tr>\n
UniProt<\/strong><\/td>\nN\/A<\/td>\n<\/tr>\n
Abbreviation<\/strong><\/td>\n2019-nCoV-Ab-IgM<\/td>\n<\/tr>\n
Alternative Names<\/strong><\/td>\nSARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein<\/td>\n<\/tr>\n
Range<\/strong><\/td>\nQualitative detection antibody<\/td>\n<\/tr>\n
Sensitivity<\/strong><\/td>\nRequest Information<\/a><\/td>\n<\/tr>\n
Sample Type<\/strong><\/td>\nSerum<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nIndirect method<\/td>\n<\/tr>\n
Analysis Method<\/strong><\/td>\nQualitative<\/td>\n<\/tr>\n
Assay Duration<\/strong><\/td>\n1-3h<\/td>\n<\/tr>\n
Sample Volume<\/strong><\/td>\n1-200 \u03bcL<\/td>\n<\/tr>\n
Detection Wavelengt<\/strong><\/td>\n450 nm<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD\/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%<\/p>\n\n\n\n\n\n\n
Sample Type<\/td>\n1\uff1a2<\/td>\n1\uff1a4<\/td>\n1\uff1a8<\/td>\n1\uff1a16<\/td>\n<\/tr>\n
Serum<\/td>\n78-89%<\/td>\n81-99%<\/td>\n92-103%<\/td>\n95-105%<\/td>\n<\/tr>\n
EDTA plasma<\/td>\n91-101%<\/td>\n90-98%<\/td>\n93-101%<\/td>\n91-98%<\/td>\n<\/tr>\n
Heparin\u00a0plasma<\/td>\n92-103%<\/td>\n93-102%<\/td>\n92-99%<\/td>\n91-101%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4eba\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\u6838\u86cb\u767dIgG\u6297\u9ad4\uff082019-nCoV-Ab-IgM\uff09ELISA\u8a66\u5291\u76d2 <\/strong>\u5957\u7d44\u5167\u5bb9<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Reagents<\/td>\nQuantity<\/td>\nReagents<\/td>\nQuantity<\/td>\n<\/tr>\n
Assay plate (96 Wells)<\/td>\n1<\/td>\nInstruction manual<\/td>\n1<\/td>\n<\/tr>\n
Standard (lyophilized)<\/td>\n2<\/td>\nSample Diluent<\/td>\n1\u00a0x 20 mL<\/td>\n<\/tr>\n
Biotin-Conjugate (concentrate\u00a0100 x)<\/td>\n1 x\u00a0120\u00a0\u03bcL<\/td>\nBiotin-Conjugate Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Streptavidin-HRP \u00a0(concentrate\u00a0100 x)<\/td>\n1 x 120\u00a0\u03bcL<\/td>\nStreptavidin-HRP Diluent<\/td>\n1 x 20\u00a0mL<\/td>\n<\/tr>\n
Wash Buffer (concentrate\u00a025\u00a0x)<\/td>\n1 x 20\u00a0mL<\/td>\nSubstrate Solution<\/td>\n1 x 12\u00a0mL<\/td>\n<\/tr>\n
Stop Solution<\/td>\n1 x 10\u00a0mL<\/td>\nAdhesive Films<\/td>\n4<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4eba\u65b0\u578b\u51a0\u72c0\u75c5\u6bd2\u6838\u86cb\u767dIgM\u6297\u9ad4\uff082019-nCoV-Ab-IgM\uff09ELISA\u8a66\u5291\u76d2 | \u8ca8\u865fAE64578HU […]<\/p>\n","protected":false},"author":3,"featured_media":33544,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[4866],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33546"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33546"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33546\/revisions"}],"predecessor-version":[{"id":33553,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33546\/revisions\/33553"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33544"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33546"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33546"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33546"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}