{"id":33532,"date":"2022-07-29T14:07:19","date_gmt":"2022-07-29T06:07:19","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33532"},"modified":"2022-08-02T15:12:01","modified_gmt":"2022-08-02T07:12:01","slug":"exkine-%e6%a0%b8%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e9%ab%98%e7%b4%94%e5%ba%a6%ef%bc%89-exkine-nuclei-extraction-kit-high-purity%e8%b2%a8%e8%99%9fktp4002","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33532","title":{"rendered":"ExKine\u2122 \u6838\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u9ad8\u7d14\u5ea6\uff09 | ExKine\u2122 Nuclei Extraction Kit (High Purity)\u8ca8\u865fKTP4002"},"content":{"rendered":"

ExKine\u2122 \u50b3\u7d71\u6838\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u9ad8\u7d14\u5ea6\uff09 | ExKine\u2122 Nuclei Extraction Kit (High Purity)\u8ca8\u865fKTP4002<\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n

\u00a0<\/strong>The preparation of an extract from nuclei is often the first step for many cell biology applications, such as in vitro apoptosis, examination of the transcriptional status of cells and a source of nuclear components (chromatin, genomic DNA, histones and nuclear RNA\/RNP).\u7d30\u80de\u6838(nuclei)\u63d0\u53d6\u7269\u7684\u88fd\u5099\u901a\u5e38\u662f\u8a31\u591a\u7d30\u80de\u751f\u7269\u5b78\u61c9\u7528(cell biology applications)\u7684\u7b2c\u4e00\u6b65\uff0c\u4f8b\u5982\u9ad4\u5916\u51cb\u4ea1(in vitro apoptosis)\u3001\u7d30\u80de\u8f49\u9304\u72c0\u614b(transcriptional status)\u7684\u6aa2\u67e5\u548c\u6838\u6210\u5206\uff08\u67d3\u8272\u8cea\u3001\u57fa\u56e0\u7d44 DNA\u3001\u7d44\u86cb\u767d\u548c\u6838 RNA\/RNP\uff09\u7684\u4f86\u6e90\u3002Abbkine ExKine\u2122 \u7d30\u80de\u6838\u63d0\u53d6\u8a66\u5291\u76d2\uff08Nuclei Extraction Kit, \u9ad8\u7d14\u5ea6\uff09\u8a2d\u8a08\u7528\u65bc\u5f9e\u54fa\u4e73\u52d5\u7269\u57f9\u990a\u7d30\u80de\u548c\u5be6\u9ad4\u7d44\u7e54\u4e2d\u88fd\u5099\u7d14\u7d30\u80de\u6838(pure nuclei)\u548c\u6613\u788e\u7d30\u80de\u6838(fragile nuclei)\u3002\u901a\u904e\u8517\u7cd6\u588a\u901a(sucrose cushion)\u904e\u8d85\u901f\u96e2\u5fc3(ultracentrifugation)\u6536\u96c6\u7d30\u80de\u6838\uff0c\u7d30\u80de\u6838\u975e\u5e38\u4e7e\u6de8\uff0c\u6c92\u6709\u6c61\u67d3\u819c(contaminating membranes)\u548c\u7d30\u80de\u8cea\u6210\u5206(cytoplasmic components)\u3002\u5206\u96e2\u7684\u7d30\u80de\u6838\u53ef\u4ee5\u5728\u96a8\u9644\u7684\u5132\u5b58\u7de9\u885d\u6db2(storage buffer)\u4e2d\u51b7\u51cd\u4fdd\u5b58\u6578\u6708\u3002<\/p>\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nExKine\u2122 Nuclei Extraction Kit (High Purity)<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Lysis Buffer
\n\u2022 1.8M Sucrose Cushion
\n\u2022 Sucrose Dilution
\n\u2022 Nuclei Storage Buffer<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Preparation of pure nuclei and fragile nuclei from mammalian cultured cells and solid tissues.
\n\u2022 Protocol conducts ultracentrifugation through a dense sucrose cushion to protect nuclei and strip away membranes and cytoplasmic contaminants.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nPerform all steps at 2\u20138 \u00b0C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

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ExKine\u2122 \u50b3\u7d71\u6838\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u9ad8\u7d14\u5ea6\uff09 | ExKine\u2122 Nuclei Extraction Kit ( […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[4862],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33532"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33532"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33532\/revisions"}],"predecessor-version":[{"id":33533,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33532\/revisions\/33533"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33532"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33532"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33532"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}