{"id":33527,"date":"2022-07-29T11:44:34","date_gmt":"2022-07-29T03:44:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33527"},"modified":"2022-08-02T15:11:50","modified_gmt":"2022-08-02T07:11:50","slug":"exkine-%e7%b2%92%e7%b7%9a%e9%ab%94%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e7%b4%b0%e8%83%9e%ef%bc%89-exkine-mitochondrion-extraction-kit-cultured-cells%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33527","title":{"rendered":"ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 | ExKine\u2122 Mitochondrion Extraction Kit (Cultured Cells)\u8ca8\u865fKTP4003"},"content":{"rendered":"

ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 | ExKine\u2122 Mitochondrion Extraction Kit (Cultured Cells)\u8ca8\u865fKTP4003<\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n

\u7c92\u7dda\u9ad4(Mitochondria)\u662f\u4e00\u7a2e\u96d9\u819c\u7d50\u5408(double-membrane-bound)\u7684\u7d30\u80de\u5668(organelle)\uff0c\u5b58\u5728\u65bc\u5927\u591a\u6578\u771f\u6838\u7d30\u80de(eukaryotic cells)\u4e2d\u3002\u7c92\u7dda\u9ad4(mitochondria)\u7684\u529f\u80fd\u662f\u63d0\u4f9b\u7d30\u80de\u80fd\u91cf(cellular energy)\u3002\u6b64\u5916\uff0c\u7c92\u7dda\u9ad4(Mitochondria)\u9084\u53c3\u8207\u5176\u4ed6\u4efb\u52d9\uff0c\u4f8b\u5982\u4fe1\u865f\u50b3\u5c0e(signaling)\u3001\u7d30\u80de\u5206\u5316(cellular differentiation)\u548c\u7d30\u80de\u6b7b\u4ea1(cell death)\uff0c\u4ee5\u53ca\u7dad\u6301\u5c0d\u7d30\u80de\u9031\u671f(cell cycle)\u548c\u7d30\u80de\u751f\u9577(cell growth)\u7684\u63a7\u5236\u3002\u7c92\u7dda\u9ad4(Mitochondria)\u8207\u591a\u7a2e\u4eba\u985e\u75be\u75c5\u6709\u95dc\uff0c\u5305\u62ec\u7c92\u7dda\u9ad4\u75be\u75c5(mitochondrial disorders)\u3001\u5fc3\u81df\u529f\u80fd\u969c\u7919(cardiacdysfunction)\u3001\u5fc3\u529b\u8870\u7aed(heart failure)\u548c\u81ea\u9589\u75c7(autism)\u3002\u7c92\u7dda\u9ad4(Mitochondria)\u53ef\u80fd\u5728\u9019\u4e9b\u7d30\u80de\u904e\u7a0b\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002Abbkine ExKine\u2122 \u7c92\u7dda\u9ad4(Mitochondria)\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u57f9\u990a\u7d30\u80de\uff09\u80fd\u5920\u5feb\u901f\u3001\u7c97\u7565\u5730\u5f9e\u57f9\u990a\u7684\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u4e2d\u5206\u96e2\u51fa\u5b8c\u6574\u7684\u7c92\u7dda\u9ad4(Mitochondria)\u3002\u8a72\u8a66\u5291\u76d2\u63d0\u4f9b\u5169\u7a2e\u65b9\u6848\uff0c\u7528\u65bc\u4f9d\u9760\u5dee\u901f\u96e2\u5fc3\u5f9e\u7d30\u80de\u6eb6\u8cea\u6210\u5206(cytosolic components relying)\u4e2d\u5206\u96e2\u7c92\u7dda\u9ad4(Mitochondria)\u3002\u7b2c\u4e00\u500b\u5354\u8b70\u63a1\u7528\u57fa\u65bc\u8a66\u5291\u7684\u65b9\u6cd5\uff0c\u5141\u8a31\u540c\u6642\u8655\u7406\u591a\u500b\u6a23\u672c\u3002\u7b2c\u4e8c\u500b\u5354\u8b70\u4f7f\u7528\u50b3\u7d71\u7684 Dounce \u5747\u8cea\u5316\uff0c\u4e26\u63d0\u4f9b\u5927\u7d04\u5169\u500d\u7684\u7c92\u7dda\u9ad4(Mitochondria)\u3002<\/p>\n

\"KTP4003<\/p>\n

Fig. 293T cells were extracted with ExKine\u2122 Mitochondrion Extraction Kit (Cultured Cells). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191), VDAC1 antibody (cat#ABP53121) and COX IV antibody (cat#A01060).<\/p>\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nExKine\u2122 Mitochondrion Extraction Kit (Cultured Cells)<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Lysis Buffer A (5\u00d7)
\n\u2022 Lysis Buffer B
\n\u2022 Lysis Buffer C
\n\u2022 Storage Buffer<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Isolate intact mitochondria from 20 million cells in less than 1 hour.
\n\u2022 Process several samples simultaneously using the reagent-based method, or obtain highest possible yield with the Dounce homogenization method.
\n\u2022 Isolated mitochondria is compatible with almost any downstream application.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nPerform all steps at 2\u20138 \u00b0C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

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ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d30\u80de\uff09 | ExKine\u2122 Mitochondrion Extraction […]<\/p>\n","protected":false},"author":3,"featured_media":33530,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[4861],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33527"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33527"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33527\/revisions"}],"predecessor-version":[{"id":33529,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33527\/revisions\/33529"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33530"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33527"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33527"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33527"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}