{"id":33521,"date":"2022-07-29T11:36:45","date_gmt":"2022-07-29T03:36:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33521"},"modified":"2023-04-19T16:36:13","modified_gmt":"2023-04-19T08:36:13","slug":"exkine-%e7%b2%92%e7%b7%9a%e9%ab%94%e6%8f%90%e5%8f%96%e8%a9%a6%e5%8a%91%e7%9b%92%ef%bc%88%e7%b5%84%e7%b9%94%ef%bc%89-exkine-mitochondrion-extraction-kit-tissue%e8%b2%a8%e8%99%9fkt","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33521","title":{"rendered":"ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d44\u7e54\uff09 | ExKine\u2122 Mitochondrion Extraction Kit (Tissue)\u8ca8\u865fKTP4004"},"content":{"rendered":"

ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d44\u7e54\uff09 | ExKine\u2122 Mitochondrion Extraction Kit (Tissue)\u8ca8\u865fKTP4004<\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/strong><\/a><\/span><\/p>\n

\u7c92\u7dda\u9ad4(Mitochondria)\u662f\u4e00\u7a2e\u96d9\u819c\u7d50\u5408(double-membrane-bound)\u7684\u7d30\u80de\u5668(organelle)\uff0c\u5b58\u5728\u65bc\u5927\u591a\u6578\u771f\u6838\u7d30\u80de(eukaryotic cells)\u4e2d\u3002\u7c92\u7dda\u9ad4(mitochondria)\u7684\u529f\u80fd\u662f\u63d0\u4f9b\u7d30\u80de\u80fd\u91cf(cellular energy)\u3002\u6b64\u5916\uff0c\u7c92\u7dda\u9ad4(Mitochondria)\u9084\u53c3\u8207\u5176\u4ed6\u4efb\u52d9\uff0c\u4f8b\u5982\u4fe1\u865f\u50b3\u5c0e(signaling)\u3001\u7d30\u80de\u5206\u5316(cellular differentiation)\u548c\u7d30\u80de\u6b7b\u4ea1(cell death)\uff0c\u4ee5\u53ca\u7dad\u6301\u5c0d\u7d30\u80de\u9031\u671f(cell cycle)\u548c\u7d30\u80de\u751f\u9577(cell growth)\u7684\u63a7\u5236\u3002\u7c92\u7dda\u9ad4(Mitochondria)\u8207\u591a\u7a2e\u4eba\u985e\u75be\u75c5\u6709\u95dc\uff0c\u5305\u62ec\u7c92\u7dda\u9ad4\u75be\u75c5(mitochondrial disorders)\u3001\u5fc3\u81df\u529f\u80fd\u969c\u7919(cardiacdysfunction)\u3001\u5fc3\u529b\u8870\u7aed(heart failure)\u548c\u81ea\u9589\u75c7(autism)\u3002\u7c92\u7dda\u9ad4(Mitochondria)\u53ef\u80fd\u5728\u9019\u4e9b\u7d30\u80de\u904e\u7a0b\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n

Abbkine ExKine\u2122 \u7dda\u7c92\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d44\u7e54\uff09\u4f7f\u7528\u5dee\u901f\u96e2\u5fc3\u6cd5\u5f9e\u8edf\u7d44\u7e54\u548c\u786c\u7d44\u7e54\u7684\u52d5\u7269\u7d44\u7e54\u4e2d\uff0c\u5feb\u901f\u7c97\u7565\u5730\u5206\u96e2\u51fa\u5b8c\u6574\u7684\u7c92\u7dda\u9ad4(Mitochondria)\u3002\u9019\u7a2e\u7c97\u7c92\u7dda\u9ad4(Mitochondria)\u88fd\u5099\u901a\u5e38\u8db3\u4ee5\u6eff\u8db3\u5927\u591a\u6578\u61c9\u7528\uff0c\u4f8b\u5982\u7814\u7a76\u7c92\u7dda\u9ad4(Mitochondria)\u547c\u5438\u3001\u7c92\u7dda\u9ad4(Mitochondria)\u819c\u96fb\u4f4d\u3001\u7d30\u80de\u51cb\u4ea1\u3001mtDNA \u548c mtRNA\uff0c\u4ee5\u53ca\u7c92\u7dda\u9ad4(Mitochondria)\u86cb\u767d\u8cea\u5206\u6790\u7b49\u3002<\/p>\n

\u9810\u8a08\u6a23\u54c1\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u61c9\u7d04\u70ba 10-25 mg\/mL<\/span><\/pre>\n
\u00a0\"KTP4004<\/strong><\/p>\n
Fig. Mouse liver tissues were extracted with ExKine\u2122 Mitochondrion Extraction Kit (Tissue). Mitochondrial (M) and Cytosolic (C) fractions were analyzed by WB using cytochrome c antibody (cat#ABM40191) and VDAC1 antibody (cat#ABP53121).<\/p>\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nExKine\u2122 Mitochondrion Extraction Kit (Tissue)<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine ExKine\u2122 Mitochondrion Extraction Kit (Tissue) enables rapid and crude isolation of of intact mitochondria from animal tissues from both soft and hard tissues using differential centrifugation. This crude mitochondrial preparation is often enough for most applications, such as study of mitochondrial respiration, mitochondria membrane potential, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling etc.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Lysis Buffer A (5\u00d7)
\n\u2022 Lysis Buffer B (5\u00d7)
\n\u2022 Storage Buffer<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Isolate intact mitochondria from animal tissues from both soft and hard tissues.
\n\u2022 Isolated mitochondria is compatible with almost any downstream application.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nPerform all steps at 2\u20138 \u00b0C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nRefer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
 <\/p>\n","protected":false},"excerpt":{"rendered":"
ExKine\u2122 \u7c92\u7dda\u9ad4\u63d0\u53d6\u8a66\u5291\u76d2\uff08\u7d44\u7e54\uff09 | ExKine\u2122 Mitochondrion Extraction […]<\/p>\n","protected":false},"author":3,"featured_media":33523,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[4860],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33521"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33521"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33521\/revisions"}],"predecessor-version":[{"id":33525,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33521\/revisions\/33525"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/33523"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33521"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33521"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33521"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}