{"id":33517,"date":"2022-07-28T17:37:44","date_gmt":"2022-07-28T09:37:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33517"},"modified":"2023-09-04T12:13:49","modified_gmt":"2023-09-04T04:13:49","slug":"chekine-%e7%b8%bd%e7%a2%b3%e6%b0%b4%e5%8c%96%e5%90%88%e7%89%a9%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-chekine-total-carbohydrate-assay-kit%e8%b2%a8%e8%99%9fktb1350","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33517","title":{"rendered":"CheKine\u2122 \u7e3d\u78b3\u6c34\u5316\u5408\u7269\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Total Carbohydrate Assay Kit\u8ca8\u865fKTB1350"},"content":{"rendered":"

CheKine\u2122 \u7e3d\u78b3\u6c34\u5316\u5408\u7269\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Total Carbohydrate Assay Kit\u8ca8\u865fKTB1350<\/span><\/strong><\/span><\/a><\/span><\/p>\n

\u5075\u6e2c\u9748\u654f\u5ea6\u00a00.1-2 mg\/mL; \u6700\u4f4e\u9748\u654f\u5ea60.1 mg\/mL<\/p>\n

\u78b3\u6c34\u5316\u5408\u7269(Carbohydrate)\u662f\u52d5\u690d\u7269\u7684\u91cd\u8981\u6210\u5206\u4e4b\u4e00\uff0c\u4e5f\u662f\u65b0\u9673\u4ee3\u8b1d(metabolism)\u7684\u4e3b\u8981\u539f\u6599\u548c\u5132\u5b58\u7269\u8cea\u3002\u7e3d\u78b3\u6c34\u5316\u5408\u7269(Total carbohydrates)\u4e3b\u8981\u662f\u6307\u5728\u6e2c\u5b9a\u689d\u4ef6\u4e0b\u53ef\u6c34\u89e3\u6210\u9084\u539f\u6027\u55ae\u91a3(reducing monosaccharides)\u7684\u9084\u539f\u6027\u8461\u8404\u7cd6(reducing glucose)\u3001\u679c\u7cd6(fructose)\u3001\u9ea5\u82bd\u7cd6(maltose)\u3001\u4e73\u7cd6(lactose)\u3001\u8517\u7cd6(sucrose)\u548c\u9ea5\u82bd\u7cd6\u4ee5\u53ca\u53ef\u80fd\u90e8\u5206\u6c34\u89e3\u7684\u6fb1\u7c89\u3002CheKine\u2122 \u7e3d\u78b3\u6c34\u5316\u5408\u7269\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08Total Carbohydrate Assay Kit, \u6bd4\u8272\u6cd5\uff09\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u3001\u65b9\u4fbf\u3001\u5feb\u901f\u7684\u6bd4\u8272\u7e3d\u78b3\u6c34\u5316\u5408\u7269(total carbohydrate)\u6aa2\u6e2c\u65b9\u6cd5\u3002\u8a66\u5291\u76d2\u539f\u7406\u662f\u5c07\u7e3d\u78b3\u6c34\u5316\u5408\u7269(total carbohydrate)\u6c34\u89e3\u6210\u9084\u539f\u7cd6(reducing sugarsthe)\uff0c\u9084\u539f\u7cd6(reducing sugarsthe)\u8207DNS\u8a66\u5291\u5728\u9e7c\u6027\u689d\u4ef6(alkaline conditions)\u4e0b\u52a0\u71b1\u9084\u539fDNS\u751f\u6210\u6c28\u57fa\u5316\u5408\u7269(amino compounds)\uff0c\u5728\u9e7c\u6027\u6eb6\u6db2(alkaline solution)\u4e2d\u5448\u7d05\u68d5\u8272\uff0c\u5728540 nm\u8655\u6709\u7279\u5fb5\u5438\u6536\u5cf0.\u5728\u4e00\u5b9a\u6fc3\u5ea6\u7bc4\u570d\u5167\uff0c\u9084\u539f\u7cd6\u542b\u91cf\u8207540 nm\u8655\u7684\u5438\u5149\u5ea6\u5448\u7dda\u6027\u95dc\u4fc2\u3002\u6839\u64da\u6a19\u6e96\u66f2\u7dda\uff0c\u53ef\u4ee5\u8a08\u7b97\u51fa\u6a23\u54c1\u4e2d\u7684\u7e3d\u78b3\u6c34\u5316\u5408\u7269(total carbohydrate)\u542b\u91cf\u3002<\/p>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/p>\n


\n\u6a23\u54c1\u88fd\u5099(Sample Preparation)
\n<\/span><\/strong><\/span>
\n1.\u690d\u7269\u52d5\u7269\u7d44\u7e54\u6a23\u54c1\uff1a
\n\u7a31\u53d60.1g\u7d44\u7e54\uff0c\u52a01mL\u9e7d\u9178\u30011.5mL\u53bb\u96e2\u5b50\u6c34\uff0c\u5747\u8cea\uff0c\u6cb8\u6c34\u6d7430min\u3002\u7136\u5f8c\u52a0\u51651mL\u6c2b\u6c27\u5316\u9209\uff0c\u6df7\u52fb\uff0c\u7528\u53bb\u96e2\u5b50\u6c34\u7a00\u91cb\u81f310mL\u3002 8,000g\u572825\u2103\u4e0b\u96e2\u5fc310\u5206\u9418\u3002\u4e0a\u6e05\u6db2\u7528\u65bc\u6e2c\u5b9a\u3002
\n2.\u8840\u6e05\u6216\u8840\u6f3f\u6a23\u54c1\uff1a\u53d60.1mL\u8840\u6f3f\uff08\u8840\u6e05\uff09\uff0c\u52a00.1mL\u9e7d\u9178\u30010.15mL\u53bb\u96e2\u5b50\u6c34\uff0c\u52fb\u6f3f\uff0c\u6cb8\u6c34\u6d7430min\u3002\u7136\u5f8c\u52a0\u51650.1mL\u6c2b\u6c27\u5316\u9209\uff0c\u6df7\u52fb\uff0c\u7528\u84b8\u993e\u6c34\u7a00\u91cb\u81f31mL\u3002 25\u00b0C\u4e0b8,000g\u96e2\u5fc310\u5206\u9418\u3002\u4f7f\u7528\u4e0a\u6e05\u6db2\u9032\u884c\u6e2c\u5b9a\u3002\u6ce8\uff1a\u5982\u679c\u6309\u86cb\u767d\u8cea\u6fc3\u5ea6\u8a08\u7b97\u542b\u91cf\uff0c\u6700\u597d\u7528ProteinQuantificationKit(BCAAssay)\uff0cCat#\uff1aKTD3001\u4f86\u5b9a\u91cf\u7e3d\u86cb\u767d\u3002<\/p>\n

<\/p>\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Total Carbohydrate Assay Kit\uff08Colorimetric\uff09<\/a><\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Hydrochloric Acid
\n\u2022 Sodium Hydroxide
\n\u2022 DNS Reagent
\n\u2022 Standard<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 simple, sensitive, rapid Total Carbohydrate detection method.
\n\u2022 compatible to animal or plant tissues, serum, plasma and other biological fluids.
\n\u2022 Provides detailed sample preparation and results calculation methods.<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nCarbohydrate is one of the important components of animal and plant, and it is also the main raw material and storage substance of metabolism. Total carbohydrates mainly refer to reducing glucose, fructose, maltose, lactose, sucrose and maltose that can be hydrolyzed into reducing monosaccharides under the measuremen tconditions and starch that can possibly partially hydrolyzed .<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122 \u7e3d\u78b3\u6c34\u5316\u5408\u7269\u6aa2\u6e2c\u8a66\u5291\u76d2 | CheKine\u2122 Total Carbohydrate Assa […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[4859],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33517"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33517"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33517\/revisions"}],"predecessor-version":[{"id":45627,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33517\/revisions\/45627"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33517"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33517"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33517"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}