{"id":33488,"date":"2022-07-25T14:20:43","date_gmt":"2022-07-25T06:20:43","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33488"},"modified":"2025-05-23T11:55:36","modified_gmt":"2025-05-23T03:55:36","slug":"human-egf-protein%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fprp100159","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33488","title":{"rendered":"Human EGF protein\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP100159"},"content":{"rendered":"

Human EGF protein\u91cd\u7d44<\/strong>\u86cb\u767d<\/strong> |<\/strong>\u8ca8\u865f<\/strong>PRP100159<\/span><\/a><\/span><\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)<\/p>\n

\"abbkine02\"<\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u4f5c\u70ba\u4e00\u7a2e\u7368\u7279\u7684\u7d30\u80de\u4fe1\u865f\u8f49\u5c0e(cell signal transduction)\u5206\u5b50\uff0c\u5728\u7d30\u80de\u589e\u6b96(cell proliferation)\u548c\u767c\u80b2(development)\u4e2d\u767c\u63ee\u8457\u91cd\u8981\u4f5c\u7528\u3002 Abbkine \u70ba\u764c\u75c7\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u5167\u5206\u6ccc\u5b78\u3001\u514d\u75ab\u5b78\u3001\u795e\u7d93\u79d1\u5b78\u3001\u86cb\u767d\u9176\u548c\u4e7e\u7d30\u80de\u7814\u7a76\u63d0\u4f9b\u7d30\u80de\u56e0\u5b50\uff0c\u5305\u62ec\u767d\u7d30\u80de\u4ecb\u7d20(interleukin)\u3001\u8da8\u5316\u56e0\u5b50(chemokine)\u3001\u96c6\u843d\u523a\u6fc0\u56e0\u5b50 (colony stimulating factor , CSF)\u3001\u5e72\u64fe\u7d20(interferon)\u548c\u8f49\u5316\u751f\u9577\u56e0\u5b50 (transforming growth factor , TGF) \u548c\u816b\u7624\u58de\u6b7b\u56e0\u5b50 (tumor necrosis factor , TNF) \u5bb6\u65cf\u3002<\/p>\n

Abbkine \u70ba\u60a8\u63d0\u4f9b\u7d30\u80de\u56e0\u5b50(Cytokine)\uff0c\u5305\u62ec\u767d\u7d30\u80de\u4ecb\u7d20\u3001\u8da8\u5316\u56e0\u5b50\u3001\u96c6\u843d\u523a\u6fc0\u56e0\u5b50 (CSF)\u3001\u5e72\u64fe\u7d20\u3001\u8f49\u5316\u751f\u9577\u56e0\u5b50 (TGF) \u548c\u816b\u7624\u58de\u6b7b\u56e0\u5b50 (TNF)\uff0c\u7528\u65bc\u764c\u75c7\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u5167\u5206\u6ccc\u5b78\u3001\u514d\u75ab\u5b78\u3001\u795e\u7d93\u79d1\u5b78\u3001\u86cb\u767d\u9176\u548c\u5e79\u7d30\u80de\u7814\u7a76\u3002\u9019\u4e9b\u7d30\u80de\u56e0\u5b50\u7d93\u6bcf\u5fae\u514b\u86cb\u767d\u8cea\u5167\u6bd2\u7d20\uff0cLAL\u6cd5\u6e2c\u5b9a\u542b\u91cf\u5c0f\u65bc1.0EU\u3002\u7d14\u5ea6\u572895%\u4ee5\u4e0a\uff0c\u6bcf\u500b\u7d30\u80de\u56e0\u5b50\u90fd\u914d\u5099\u4e861 mL\u8207\u7d30\u80de\u56e0\u5b50\u6700\u76f8\u5bb9\u7684\u518d\u6eb6\u89e3\u7de9\u885d\u6db2\uff0c\u56e0\u6b64\u518d\u6eb6\u89e3\u5f8c\u7522\u54c1\u6027\u80fd\u66f4\u7a69\u5b9a\u3002\u904e\u56b4\u683c\u9a57\u8b49\uff0c\u53ef\u6eff\u8db3\u958b\u767c\u4eba\u54e1\u5c0d\u9ad8\u8cea\u91cf\u3001\u9ad8\u751f\u7269\u6d3b\u6027\u548c\u6709\u7af6\u722d\u529b\u7684\u50f9\u683c\u7684\u9700\u6c42\u3002<\/p>\n

(Animal-Free) Abbkine\u7d30\u80de\u57f9\u990a\u7269\uff08\u771f\u6838\u7d30\u80de\uff09\u7684\u975e\u52d5\u7269\u7d30\u80de\u56e0\u5b50(Animal-free cytokines)\u662f\u5229\u7528\u975e\u52d5\u7269(non-animal)\u539f\u6599\u548c\u8868\u9054\u7cfb\u7d71\u751f\u7522\u7684\uff0c\u8868\u9054\u7cfb\u7d71\u4e2d\u7684\u57f9\u990a\u57fa\u662f\u7121\u8840\u6e05\u3001\u7121\u52d5\u7269\u3001\u5316\u5b78\u6210\u5206\u900f\u660e\u7684\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong><\/p>\n

EGF \u662f EGF \u86cb\u767d\u5bb6\u65cf(EGF-family)\u7684\u5275\u59cb\u6210\u54e1\u3002\u8a72\u86cb\u767d\u8cea\u5bb6\u65cf\u7684\u6210\u54e1\u5177\u6709\u9ad8\u5ea6\u76f8\u4f3c\u7684\u7d50\u69cb(structural)\u548c\u529f\u80fd(functional)\u7279\u5fb5\u3002 EGF \u5305\u542b 9 \u500b EGF \u6a23\u7d50\u69cb\u57df\u548c 9 \u500b LDL \u53d7\u9ad4 B \u985e\u91cd\u8907\u5e8f\u5217\u3002\u4eba EGF \u662f\u4e00\u7a2e 6045-Da \u7684\u86cb\u767d\u8cea\uff0c\u5177\u6709 53 \u500b\u6c28\u57fa\u9178\u6b98\u57fa(amino acid residues)\u548c\u4e09\u500b\u5206\u5b50\u5167\u4e8c\u786b\u9375\u3002\u4f5c\u70ba\u4e00\u7a2e\u4f4e\u5206\u5b50\u591a\u80bd(low-molecular-weight polypeptide)\uff0cEGF\u6700\u521d\u662f\u5f9e\u5c0f\u9f20\u981c\u4e0b\u817a(submandibular gland)\u4e2d\u7d14\u5316\u800c\u4f86\uff0c\u6b64\u5f8c\u5728\u5305\u62ec\u981c\u4e0b\u817a(submandibular gland)\u3001\u816e\u817a(parotid gland)\u5728\u5167\u7684\u591a\u7a2e\u4eba\u9ad4\u7d44\u7e54\u4e2d\u5747\u6709\u767c\u73fe\u3002\u5b83\u4e5f\u5b58\u5728\u65bc\u4eba\u985e\u8840\u5c0f\u677f(platelets)\u3001\u5de8\u566c\u7d30\u80de(macrophages)\u3001\u5c3f\u6db2(urine0\u3001\u553e\u6db2(saliva)\u3001\u725b\u5976(milk)\u548c\u8840\u6f3f(plasma)\u4e2d\u3002 EGF \u662f\u4e00\u7a2e\u751f\u9577\u56e0\u5b50\uff0c\u53ef\u5728\u9ad4\u5167\u548c\u9ad4\u5916\u523a\u6fc0\u5404\u7a2e\u8868\u76ae(epidermal)\u548c\u4e0a\u76ae\u7d44\u7e54(epithelial)\u7684\u751f\u9577\uff0c\u4ee5\u53ca\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u67d0\u4e9b\u6210\u7e96\u7dad\u7d30\u80de(fibroblasts)\u7684\u751f\u9577\u3002\u5b83\u5c0e\u81f4\u7d30\u80de\u589e\u6b96(cellular proliferation)\u3001\u5206\u5316(differentiation)\u548c\u5b58\u6d3b(survival)\u3002\u553e\u6db2 EGF (Salivary EGF)\u4f3c\u4e4e\u4e5f\u53d7\u81b3\u98df\u7121\u6a5f\u7898(dietary inorganic iodine)\u7684\u8abf\u7bc0\uff0c\u5728\u7dad\u6301\u53e3\u98df\u7ba1(oro-esophageal)\u548c\u80c3\u7d44\u7e54\u5b8c\u6574\u6027(gastric tissue integrity)\u65b9\u9762\u4e5f\u8d77\u8457\u91cd\u8981\u7684\u751f\u7406\u4f5c\u7528\u3002 EGF\u901a\u904e\u8207\u7d30\u80de\u8868\u9762\u7684\u8868\u76ae\u751f\u9577\u56e0\u5b50\u53d7\u9ad4(epidermal growth factor receptor)\u4ee5\u9ad8\u89aa\u548c\u529b\u7d50\u5408\u4f75\u523a\u6fc0\u53d7\u9ad4\u7684\u5167\u5728\u86cb\u767d\u916a\u6c28\u9178\u6fc0\u9176\u6d3b\u6027(intrinsic protein-tyrosine kinase activity)\u800c\u8d77\u4f5c\u7528\u3002\u53cd\u904e\u4f86\uff0c\u916a\u6c28\u9178\u6fc0\u9176\u6d3b\u6027\u555f\u52d5\u4fe1\u865f\u8f49\u5c0e\u7d1a\u806f\uff0c\u5c0e\u81f4\u7d30\u80de\u5167\u767c\u751f\u5404\u7a2e\u751f\u5316\u8b8a\u5316\u2014\u2014\u7d30\u80de\u5167\u9223\u6c34\u5e73\u5347\u9ad8(intracellular calcium levels)\u3001\u7cd6\u9175\u89e3(glycolysis)\u548c\u86cb\u767d\u8cea\u5408\u6210(protein synthesis)\u589e\u52a0\uff0c\u4ee5\u53ca\u67d0\u4e9b\u57fa\u56e0\u7684\u8868\u9054\u589e\u52a0\uff0c\u5305\u62ecEGFR\u2014\u2014\u6700\u7d42\u5c0e\u81f4 DNA \u5408\u6210(DNA synthesis)\u548c\u7d30\u80de\u589e\u6b96(cell proliferation)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nHuman EGF protein<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nPRP100159<\/td>\n<\/tr>\n
Sequence<\/strong><\/td>\nAmino acid sequence derived from mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.<\/td>\n<\/tr>\n
Activity<\/strong><\/td>\nMeasured in a cell proliferation assay using Balb\/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 pg\/mL.<\/td>\n<\/tr>\n
Protein length<\/strong><\/td>\nThe recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n> 92 % as determined by SDS-PAGE<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nEpidermal Growth Factor; HOMG4; URG; Pro-epidermal growth factor; EGF<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLyophilized from sterile PBS, pH 7.4<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n6.3 kDa<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nAlways centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human EGF protein in sterile ddH2O not less than 100\u00b5g\/ml, which can then be further diluted to other aqueous solutions.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nLyophilized Human EGF proteinshould be stored desiccated below -20\u00b0C. Upon reconstitution, the protein should be stored at 4\u00b0C between 2-7 days and for future use below -20\u00b0C. For long term storage it is recommended to add a carrier protein (0.52% HSA or BSA). Please prevent freeze-thaw cycles.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Human EGF protein\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP100159 \u7d30\u80de\u56e0\u5b50(Cytokine) \u7d30\u80de\u56e0\u5b50( […]<\/p>\n","protected":false},"author":3,"featured_media":29550,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[4856],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33488"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33488"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33488\/revisions"}],"predecessor-version":[{"id":51219,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33488\/revisions\/51219"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29550"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33488"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33488"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}