{"id":33485,"date":"2022-07-25T11:59:39","date_gmt":"2022-07-25T03:59:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33485"},"modified":"2022-10-24T17:44:30","modified_gmt":"2022-10-24T09:44:30","slug":"human-il-4-protein%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-%e8%b2%a8%e8%99%9fprp100273","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33485","title":{"rendered":"Human IL-4 protein\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP100273"},"content":{"rendered":"

Human IL-4 protein\u91cd\u7d44<\/strong>\u86cb\u767d<\/strong> |<\/strong>\u8ca8\u865f<\/strong>PRP100273<\/a><\/span><\/p>\n

\"abbkine02\"<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)<\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u4f5c\u70ba\u4e00\u7a2e\u7368\u7279\u7684\u7d30\u80de\u4fe1\u865f\u8f49\u5c0e(cell signal transduction)\u5206\u5b50\uff0c\u5728\u7d30\u80de\u589e\u6b96(cell proliferation)\u548c\u767c\u80b2(development)\u4e2d\u767c\u63ee\u8457\u91cd\u8981\u4f5c\u7528\u3002 Abbkine \u70ba\u764c\u75c7\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u5167\u5206\u6ccc\u5b78\u3001\u514d\u75ab\u5b78\u3001\u795e\u7d93\u79d1\u5b78\u3001\u86cb\u767d\u9176\u548c\u4e7e\u7d30\u80de\u7814\u7a76\u63d0\u4f9b\u7d30\u80de\u56e0\u5b50\uff0c\u5305\u62ec\u767d\u7d30\u80de\u4ecb\u7d20(interleukin)\u3001\u8da8\u5316\u56e0\u5b50(chemokine)\u3001\u96c6\u843d\u523a\u6fc0\u56e0\u5b50 (colony stimulating factor , CSF)\u3001\u5e72\u64fe\u7d20(interferon)\u548c\u8f49\u5316\u751f\u9577\u56e0\u5b50 (transforming growth factor , TGF) \u548c\u816b\u7624\u58de\u6b7b\u56e0\u5b50 (tumor necrosis factor , TNF) \u5bb6\u65cf\u3002<\/p>\n

Abbkine \u70ba\u60a8\u63d0\u4f9b\u7d30\u80de\u56e0\u5b50(Cytokine)\uff0c\u5305\u62ec\u767d\u7d30\u80de\u4ecb\u7d20\u3001\u8da8\u5316\u56e0\u5b50\u3001\u96c6\u843d\u523a\u6fc0\u56e0\u5b50 (CSF)\u3001\u5e72\u64fe\u7d20\u3001\u8f49\u5316\u751f\u9577\u56e0\u5b50 (TGF) \u548c\u816b\u7624\u58de\u6b7b\u56e0\u5b50 (TNF)\uff0c\u7528\u65bc\u764c\u75c7\u3001\u7d30\u80de\u51cb\u4ea1\u3001\u5167\u5206\u6ccc\u5b78\u3001\u514d\u75ab\u5b78\u3001\u795e\u7d93\u79d1\u5b78\u3001\u86cb\u767d\u9176\u548c\u5e79\u7d30\u80de\u7814\u7a76\u3002\u9019\u4e9b\u7d30\u80de\u56e0\u5b50\u7d93\u6bcf\u5fae\u514b\u86cb\u767d\u8cea\u5167\u6bd2\u7d20\uff0cLAL\u6cd5\u6e2c\u5b9a\u542b\u91cf\u5c0f\u65bc1.0EU\u3002\u7d14\u5ea6\u572895%\u4ee5\u4e0a\uff0c\u6bcf\u500b\u7d30\u80de\u56e0\u5b50\u90fd\u914d\u5099\u4e861 mL\u8207\u7d30\u80de\u56e0\u5b50\u6700\u76f8\u5bb9\u7684\u518d\u6eb6\u89e3\u7de9\u885d\u6db2\uff0c\u56e0\u6b64\u518d\u6eb6\u89e3\u5f8c\u7522\u54c1\u6027\u80fd\u66f4\u7a69\u5b9a\u3002\u904e\u56b4\u683c\u9a57\u8b49\uff0c\u53ef\u6eff\u8db3\u958b\u767c\u4eba\u54e1\u5c0d\u9ad8\u8cea\u91cf\u3001\u9ad8\u751f\u7269\u6d3b\u6027\u548c\u6709\u7af6\u722d\u529b\u7684\u50f9\u683c\u7684\u9700\u6c42\u3002<\/p>\n

(Animal-Free) Abbkine\u7d30\u80de\u57f9\u990a\u7269\uff08\u771f\u6838\u7d30\u80de\uff09\u7684\u975e\u52d5\u7269\u7d30\u80de\u56e0\u5b50(Animal-free cytokines)\u662f\u5229\u7528\u975e\u52d5\u7269(non-animal)\u539f\u6599\u548c\u8868\u9054\u7cfb\u7d71\u751f\u7522\u7684\uff0c\u8868\u9054\u7cfb\u7d71\u4e2d\u7684\u57f9\u990a\u57fa\u662f\u7121\u8840\u6e05\u3001\u7121\u52d5\u7269\u3001\u5316\u5b78\u6210\u5206\u900f\u660e\u7684\u3002<\/p>\n

\u00a0\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/strong><\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u767d\u7d30\u80de\u4ecb\u7d204(Interleukin-4)\uff0c\u53c8\u7a31IL-4\uff0c\u662f\u4e00\u7a2e\u5206\u6ccc\u86cb\u767d(secreted protein)\uff0c\u5c6c\u65bcIL-4\/IL-13\u5bb6\u65cf\u3002 Interleukin-4 \/ IL-4 \u5177\u6709\u8a31\u591a\u751f\u7269\u5b78\u4f5c\u7528\uff0c\u5305\u62ec\u523a\u6fc0\u6d3b\u5316\u7684 B \u7d30\u80de(B-cell)\u548c T \u7d30\u80de\u589e\u6b96(T-cell proliferation)\u3002\u5b83\u589e\u5f37 IgE \u548c IgG1 \u7684\u5206\u6ccc\u548c\u7d30\u80de\u8868\u9762\u8868\u9054(cell surface expression)\u3002 Interleukin-4 \/ IL-4 \u9084\u8abf\u7bc0\u6dcb\u5df4\u7d30\u80de(lymphocytes)\u548c\u55ae\u6838\u7d30\u80de(monocytes)\u4e0a IgE (CD23) \u7684\u4f4e\u89aa\u548c\u529b Fc \u53d7\u9ad4\u7684\u8868\u9054\u3002\u767d\u7d30\u80de\u4ecb\u7d20 4 \u5c0d\u65bc\u5c07 B \u7d30\u80de\u8f49\u63db\u70ba IgE \u6297\u9ad4\u7522\u751f\u548c T \u8f14\u52a9 (Th) \u7d30\u80de\u5411 Th2 \u8868\u578b(Th2 phenotyp)\u6210\u719f\u81f3\u95dc\u91cd\u8981\u3002\u5b83\u53c3\u8207\u81f3\u5c11\u5e7e\u500b B \u7d30\u80de\u6fc0\u6d3b\u904e\u7a0b(B-cell activation processes)\u4ee5\u53ca\u5176\u4ed6\u7d30\u80de\u985e\u578b\u3002\u7136\u800c\uff0c\u7814\u7a76\u8868\u660e\uff0c\u8207 IL-4 \u53d7\u9ad4 \u03b1 \u7d50\u5408\u7684\u5c0f\u9f20 IL-4 \u86cb\u767d (QY) \u7684\u96d9\u7a81\u8b8a\u9ad4 (Q116D\u3001Y119D) \u8c37\u6c28\u9170\u80fa 116 \u548c\u916a\u6c28\u9178 119(tyrosine 119) \u4ee5\u5291\u91cf\u4f9d\u8cf4\u6027\u65b9\u5f0f\u5b8c\u5168\u6291\u5236 IL -4 \u8a98\u5c0e\u8102\u591a\u91a3\u523a\u6fc0(lipopolysaccharide-stimulated)\u7684\u9f20\u813e B \u7d30\u80de\u3001\u9f20 T \u7d30\u80de\u7cfb CTLL-2 \u548c\u9f20\u524d B \u7d30\u80de\u7cfb BA\/F3 \u7684\u589e\u6b96\u3002 QY \u9084\u901a\u904e\u8102\u591a\u91a3\u523a\u6fc0\u7684\u5c0f\u9f20\u813e B \u7d30\u80de\u6291\u5236 IL-4 \u523a\u6fc0\u7684 CD23 \u8868\u9054\u4e0a\u8abf\uff0c\u4e26\u6d88\u9664 IL-4 \u523a\u6fc0\u7684 BA\/F3 \u7d30\u80de\u4e2d\u8f49\u9304\u56e0\u5b50 Stat6 \u548c\u916a\u6c28\u9178\u6fc0\u9176 Jak3 \u7684\u916a\u6c28\u9178\u78f7\u9178\u5316(abolished tyrosine phosphorylation)\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
<\/td>\n\u7522\u54c1\u8ca8\u865f<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/u><\/strong><\/a> \u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/u><\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n412 nm \u00a02-200 \u00b5g\/mL<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n

\u8ca8\u865fKTB3010<\/a><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 340 nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1542<\/a><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

absorbance at 270 nm<\/p>\n

0.3125-20 \u03bcmoL\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1541<\/a><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

absorbance at 760 nm<\/p>\n

0.0156-1 mg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB2250<\/a><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

Absorption peak at 500nm<\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/td>\n<\/tr>\n

\u8ca8\u865fKTB2260<\/a><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

Absorption peak at 500nm<\/p>\n

Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1290<\/a><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 340 nm.<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1560<\/a><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 412 nm.<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1390<\/a><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

absorption at 660 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong>\u00a0<\/u><\/strong><\/a><\/td>\n5ug\/20ug\/100ug\/1mg<\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>PRP100273<\/a><\/td>\nHuman IL-4 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/td>\n20ug\/100ug\/1mg<\/td>\nED50 for this effect is 0.05-0.2 ng\/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 \u00d7 10E4 IU\/\u03bcg.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Human IL-4 protein\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP100273 \u7d30\u80de\u56e0\u5b50(Cytokine) \u7d30\u80de\u56e0\u5b50 […]<\/p>\n","protected":false},"author":3,"featured_media":29550,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[4857],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33485"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33485"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33485\/revisions"}],"predecessor-version":[{"id":35279,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33485\/revisions\/35279"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29550"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33485"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33485"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33485"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}