{"id":33174,"date":"2022-07-12T11:21:32","date_gmt":"2022-07-12T03:21:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33174"},"modified":"2023-05-18T13:26:02","modified_gmt":"2023-05-18T05:26:02","slug":"%e7%a1%ab%e9%86%87%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-thiol-assay-kit%e8%b2%a8%e8%99%9f-abx298894","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33174","title":{"rendered":"\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2| Thiol Assay Kit\u8ca8\u865f abx298894"},"content":{"rendered":"

\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2| Thiol Assay Kit\u8ca8\u865f\u00a0abx298894<\/span><\/strong><\/span><\/a><\/span><\/p>\n

Detection Range: 9.91 \u03bcmol\/L – 1000 \u03bcmol\/L
\nSensitivity: 9.91 \u03bcmol\/L<\/p>\n

\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2(Thiol Assay Kit, abx298894) \u662f\u4e00\u7a2e\u6aa2\u6e2c\u8a66\u5291\u76d2\uff0c\u4ee5ELISA \u5100\u5668<\/a> \u5b9a\u91cf\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d44\u7e54\u52fb\u6f3f(tissue homogenates)\u4e2d\u7684\u786b\u9187\u6fc3\u5ea6\u3002\u5df0\u57fa\u5316\u5408\u7269(Sulfhydryl compounds)\u5728\u4e2d\u6027\u6216\u9e7c\u6027\u689d\u4ef6\u4e0b\u82075,5′-\u4e8c\u786b\u4ee3\u96d9(2-nitrobenzoic acid , 2-\u785d\u57fa\u82ef\u7532\u9178)\u53cd\u61c9\u751f\u6210\u9ec3\u8272\u7522\u7269\uff0c\u5176\u6700\u5927\u5438\u6536\u5cf0\u5728412 nm\u3002\u5b58\u5728\u65bc\u6e38\u96e2\u534a\u80f1\u6c28\u9178\u3001\u7a40\u80f1\u7518\u80bd (GSH) \u548c\u86cb\u767d\u8cea\u4e2d\u534a\u80f1\u6c28\u9178\u6b98\u57fa\u4e2d\u7684\u786b\u9187\u57fa\u5718\uff08\u5df0\u57fa\uff09\u53c3\u8207\u8a31\u591a\u751f\u7269\u904e\u7a0b\u3002\u5169\u500b\u534a\u80f1\u6c28\u9178\u6b98\u57fa( cysteine residues)\u7684\u786b\u9187\u57fa\u5718\u751f\u6210\u7684\u4e8c\u786b\u9375\u88ab\u6c27\u5316\u6709\u52a9\u65bc\u5f62\u6210\u86cb\u767d\u8cea\u7684\u4e09\u7d1a\u6216\u56db\u7d1a\u7d50\u69cb\u3002Abbexa \u7684\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2(Thiol Assay Kit, abx298894) \u662f\u4e00\u7a2e\u5feb\u901f\u3001\u65b9\u4fbf\u3001\u9748\u654f\u7684\u786b\u9187\u6e2c\u91cf\u548c\u8a08\u7b97\u65b9\u6cd5\u5c08\u6ce8\u3002\u5df0\u57fa\u5316\u5408\u7269(Sulfyydryl compounds)\u5728\u4e2d\u6027\u6216\u9e7c\u6027\u689d\u4ef6\u4e0b\u8207NBA\uff082-\u785d\u57fa\u82ef\u7532\u9178\uff09\u53cd\u61c9\u751f\u6210\u7522\u751f\u9ec3\u8272\u7522\u7269\u4e26\u5728 412 nm \u8655\u6709\u5438\u6536\u6700\u5927\u503c\u3002\u5438\u5149\u5ea6\u61c9\u5728 412nm\u3002\u984f\u8272\u7684\u5f37\u5ea6\u8207\u786b\u9187\u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002<\/p>\n

\"abbexa2\"<\/a><\/p>\n

\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2\u64cd\u4f5c\u6d41\u7a0b<\/span><\/strong><\/span><\/p>\n

A. Preparation of samples and reagents<\/strong><\/span><\/p>\n

1. Samples
\nIsolate the test samples soon after collecting and analyze immediately or aliquot and store at -20\u00b0C or -80\u00b0C for
\nlong term storage. Avoid multiple freeze-thaw cycles.
\nThe following sample preparation methods are intended as a guide and may be adjusted as required depending
\non the specific samples used.<\/p>\n

\u2022 Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube
\nundisturbed in a vertical position overnight at 4\u00b0C or at room temperature for up to 1 hr. Centrifuge at
\napproximately 2000 \u00d7 g for 15 mins at 4\u00b0C. If a precipitate appears, centrifuge again. Take the supernatant,
\nkeep on ice and assay immediately, or aliquot and store at -80\u00b0C for up to 1 month.<\/p>\n

\u2022 Plasma: Collect plasma using heparin as the anticoagulant. Centrifuge for 10 mins at 1000-2000 x g at 4\u00b0C,
\nwithin 30 mins of collection. If precipitate appears, centrifuge again. Avoid hemolytic samples. Take the
\nsupernatant (avoid taking the middle layer containing white blood cells and platelets), keep on ice and assay
\nimmediately, or aliquot and store at -80\u00b0C for up to 1 month.<\/p>\n

\u2022 Tissue Homogenates: Weigh the tissue homogenate. For each 1 g of homogenate, add 9 ml Normal saline.
\nHomogenize by hand, using a mechanical homogenizer, or by ultrasonication. Centrifuge the homogenate at
\n10,000 x g at 4\u00b0C for 10 min. Collect the supernatant and assay immediately. The protein concentration in
\nthe supernatant should be determined separately<\/p>\n

Thiol Assay Kit is an assay kit designed for the detection and quantification of Thiol concentrations in serum, plasma and tissue homogenates using a microplate reader. Sulfhydryl compounds react with 5,5′-dithiobis(2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which has a maximum absorption peak at 412 nm.<\/p>\n\n\n\n\n\n\n\n\n\n\n
Target<\/td>\nThiol<\/td>\n<\/tr>\n
Reactivity<\/td>\nGeneral (All species)<\/td>\n<\/tr>\n
Storage<\/td>\nShipped at 4 \u00b0C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.<\/td>\n<\/tr>\n
Validity<\/td>\nThe validity for this kit is 12 months.<\/td>\n<\/tr>\n
Detection Method<\/td>\nColorimetric<\/td>\n<\/tr>\n
Sample Type<\/td>\nSerum, plasma and tissue homogenates.<\/td>\n<\/tr>\n
Availability<\/td>\nShipped within 5-10 working days.<\/td>\n<\/tr>\n
Note<\/td>\nThis product is for research use only.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n

\u8840\u5c3f\u7d20\u6c2e\uff08Blood Urea Nitrogen , BUN\uff09\u6aa2\u6e2c<\/a> | \u808c\u9150\u6aa2\u6e2c\u8a66\u5291\u76d2(Creatinine Assay Kit)\u00a0<\/a> | UA\u5c3f\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a> | Calcium\u9223\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a> | \u9382\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u5206\u6790\u54c1\u9805<\/td>\n<\/tr>\n
abx090684<\/a><\/td>\n\u8840\u5c3f\u7d20\u6c2e\uff08Blood Urea Nitrogen , BUN\uff09\u6aa2\u6e2c<\/a><\/td>\n<\/tr>\n
abx298944<\/a><\/td>\n\u808c\u9150\u6aa2\u6e2c\u8a66\u5291\u76d2(Creatinine Assay Kit)\u00a0<\/a><\/td>\n<\/tr>\n
abx298824<\/a><\/td>\nUA\u5c3f\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
abx298871<\/a><\/td>\nCalcium\u9223\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
abx298873<\/a><\/td>\n\u9382\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
abx298918<\/a><\/td>\n\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2| Nitrite Assay Kit\u8ca8\u865fabx298918<\/a><\/td>\n<\/tr>\n
abx298986<\/a><\/td>\n\u7e3d\u78b3\u6c34\u5316\u5408\u7269\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
abx096001<\/a><\/td>\nALT\u4e19\u6c28\u9178\u8f49\u6c28\u9176\u6aa2\u6e2c\u8a66\u5291\u76d2| Alanine Transaminase assay kit<\/a><\/td>\n<\/tr>\n
abx294012<\/a><\/td>\n\u9ad3\u904e\u6c27\u5316\u7269\u9176 (MPO) \u6aa2\u6e2c\u8a66\u5291\u76d2| Myeloperoxidase (MPO) Assay Kit<\/a><\/td>\n<\/tr>\n
\u00a0abx298894<\/a><\/td>\n\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u786b\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2| Thiol Assay Kit\u8ca8\u865f\u00a0abx298894 Detection Range: 9 […]<\/p>\n","protected":false},"author":3,"featured_media":29387,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[797],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33174"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33174"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33174\/revisions"}],"predecessor-version":[{"id":42477,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33174\/revisions\/42477"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29387"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33174"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33174"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33174"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}