{"id":33157,"date":"2022-07-12T09:55:38","date_gmt":"2022-07-12T01:55:38","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33157"},"modified":"2023-11-14T16:23:58","modified_gmt":"2023-11-14T08:23:58","slug":"%e4%ba%9e%e7%a1%9d%e9%85%b8%e9%b9%bd%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-nitrite-assay-kit%e8%b2%a8%e8%99%9fabx298918","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33157","title":{"rendered":"\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2| Nitrite Assay Kit\u8ca8\u865fabx298918"},"content":{"rendered":"

\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2| Nitrite Assay Kit\u8ca8\u865fabx298918<\/a><\/span><\/strong><\/p>\n

Nitrite Assay Kit<\/span><\/p>\n

Detection Range: 1.36 \u03bcmol\/L \u2013 500 \u03bcmol\/L<\/span>
\nSensitivity: 1.36 \u03bcmol\/L<\/span><\/p>\n

\u4e9e\u785d\u9178\u9e7d(Nitrites )\u548c\u785d\u9178\u9e7d(Nitrates )\u662f\u6c2e\u5faa\u74b0(nitrogen cycle)\u4e2d\u7522\u751f\u7684\u666e\u904d\u5b58\u5728\u7684\u96e2\u5b50\uff0c\u5ee3\u6cdb\u7528\u65bc\u5316\u5b78(chemical)\u548c\u88fd\u85e5\u884c\u696d(pharmaceutical industries)\u3002\u4e9e\u785d\u9178\u9e7d( Nitrites )\u5728\u6c34\u4e2d\u5f62\u6210\u5171\u8edb\u9178(conjugate acid)\uff0c\u5177\u6709\u6c27\u5316\u9084\u539f\u6d3b\u6027(redox activity)\u3002\u5728\u6b64\u6e2c\u5b9a\u4e2d\uff0c\u4e9e\u785d\u9178\u9e7d\u8207\u6aa2\u6e2c\u8a66\u5291\u53cd\u61c9\u751f\u6210\u6dfa\u7d05\u8272\u5076\u6c2e\u5316\u5408\u7269( light-red azo-compound)\u3002Abbexa \u7684\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2(Abbexa\u2019s Nitrite Assay Kit, abx298918) \u662f\u4e00\u7a2e\u5feb\u901f\u3001\u65b9\u4fbf\u4e14\u9748\u654f\u7684\u6e2c\u91cf\u548c\u8a08\u7b97\u4e9e\u785d\u9178\u9e7d\u6fc3\u5ea6(Nitrite concentration)\u7684\u65b9\u6cd5\u3002\u4ee5\u5206\u5149\u5149\u5ea6\u8a08\u5b9a\u91cf\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d44\u7e54\u52fb\u6f3f(tissue homogenates)\u3001\u7d30\u80de\u88c2\u89e3\u7269(cell culture supernatants)\u3001\u553e\u6db2(saliva)\u4e2d\u7684\u4e9e\u785d\u9178\u9e7d(Nitrite)\u6fc3\u5ea6\u3002\u4e9e\u785d\u9178\u9e7d(Nitrite)\u7684\u6fc3\u5ea6\u53ef\u4ee5\u901a\u904e\u6e2c\u91cf 550 nm \u8655\u7684\u5438\u5149\u5ea6\u4f86\u9593\u63a5\u8a08\u7b97\u3002\u5076\u6c2e\u5316\u5408\u7269\u7522\u7269\u5728 550 nm \u8655\u5177\u6709\u6700\u5927\u5438\u5149\u5ea6\u3002\u984f\u8272\u7684\u5f37\u5ea6\u8207\u4e9e\u785d\u9178\u9e7d\u6fc3\u5ea6\u6210\u6b63\u6bd4\u3002\u672a\u77e5\u6a23\u54c1\uff0c\u9700\u6e2c\u8a66\u4e9e\u785d\u9178\u9e7d(Nitrite)\u6fc3\u5ea6\u662f\u5426\u5728\u7dda\u6027\u7bc4\u570d\u5167\u3002<\/p>\n

\"abbexa2\"<\/a><\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Target<\/td>\nNitrite<\/td>\n<\/tr>\n
Reactivity<\/td>\nGeneral (All species)<\/td>\n<\/tr>\n
Storage<\/td>\nShipped at 4 \u00b0C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.<\/td>\n<\/tr>\n
Validity<\/td>\nThe validity for this kit is 6 months.<\/td>\n<\/tr>\n
Detection Method<\/td>\nColorimetric<\/td>\n<\/tr>\n
Sample Type<\/td>\nSerum, plasma, tissue homogenates, cell lysates, cell culture supernatants and saliva.<\/td>\n<\/tr>\n
Availability<\/td>\nShipped within 5-10 working days.<\/td>\n<\/tr>\n
Note<\/td>\nThis product is for research use only.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2\u64cd\u4f5c\u624b\u518a (\u6a23\u54c1\u88fd\u5099)<\/span><\/strong><\/span><\/p>\n

A. \u6a23\u54c1\u548c\u8a66\u5291\u7684\u88fd\u5099 (Preparation of samples and reagents)
\n\u6536\u96c6\u5f8c\u7acb\u5373\u5206\u96e2\u6e2c\u8a66\u6a23\u54c1\u4e26\u7acb\u5373\u5206\u6790\uff0c\u6216\u7b49\u5206\u4e26\u5132\u5b58\u5728 -20\u00b0C \u6216 -80\u00b0C \u4ee5\u9032\u884c\u9577\u671f\u5132\u5b58\u3002\u907f\u514d\u591a\u6b21\u51cd\u878d\u5faa\u74b0\u3002\u4ee5\u4e0b\u6a23\u54c1\u88fd\u5099\u65b9\u6cd5\u50c5\u4f9b\u53c3\u8003\uff0c\u53ef\u6839\u64da\u4f7f\u7528\u7684\u5177\u9ad4\u6a23\u54c1\u6309\u9700\u8981\u9032\u884c\u8abf\u6574\u3002<\/p>\n

    \n
  1. \u8840\u6e05\u6a23\u54c1\u61c9\u6536\u96c6\u5230\u8840\u6e05\u5206\u96e2\u7ba1\u4e2d\u3002\u5c07\u7ba1\u5728 4\u00b0C \u4e0b\u5782\u76f4\u653e\u7f6e\u904e\u591c\u6216\u5728\u5ba4\u6eab\u4e0b\u653e\u7f6e\u6700\u591a 1 \u5c0f\u6642\uff0c\u4ee5\u51dd\u56fa\u8840\u6e05\u3002\u5728 4\u00b0C \u4e0b\u4ee5\u5927\u7d04 2000 \u00d7 g \u96e2\u5fc3 15 \u5206\u9418\u3002\u5982\u679c\u51fa\u73fe\u6c89\u6fb1\uff0c\u518d\u6b21\u96e2\u5fc3\u3002\u53d6\u4e0a\u6e05\u6db2\uff0c\u7f6e\u65bc\u51b0\u4e0a\u4e26\u7acb\u5373\u6e2c\u5b9a\uff0c\u6216\u7b49\u5206\u4e26\u5728 -80\u00b0C \u4e0b\u5132\u5b58\u6700\u591a 1 \u500b\u6708\u3002<\/li>\n
  2. \u8840\u6f3f\u4f7f\u7528\u809d\u7d20\u4f5c\u70ba\u6297\u51dd\u5291\u6536\u96c6\u8840\u6f3f\u3002\u5728\u6536\u96c6\u5f8c 30 \u5206\u9418\u5167\uff0c\u5728 4\u00b0C \u4e0b\u4ee5 700-1000 \u00d7 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u5982\u679c\u51fa\u73fe\u6c89\u6fb1\uff0c\u518d\u6b21\u96e2\u5fc3\u3002\u907f\u514d\u6eb6\u8840\u6a23\u54c1\u3002\u53d6\u4e0a\u6e05\u6db2\uff08\u907f\u514d\u53d6\u542b\u6709\u767d\u7d30\u80de\u548c\u8840\u5c0f\u677f\u7684\u4e2d\u9593\u5c64\uff09\uff0c\u7f6e\u65bc\u51b0\u4e0a\u4e26\u7acb\u5373\u6e2c\u5b9a\uff0c\u6216\u5206\u88dd\u4e26\u5728 -80\u00b0C \u4e0b\u5132\u5b58\u6700\u591a 1 \u500b\u6708\u3002<\/li>\n
  3. \u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\uff1a\u4ee5 3100 \u00d7 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u53d6\u4e0a\u6e05\u6db2\uff0c\u7f6e\u65bc\u51b0\u4e0a\uff0c\u7acb\u5373\u6e2c\u5b9a\u3002\u5982\u679c\u4e0a\u6e05\u6db2\u4e2d\u6709\u6c89\u6fb1\uff0c\u518d\u6b21\u96e2\u5fc3<\/li>\n
  4. \u7d44\u7e54\u52fb\u6f3f(Cell lysates)\u7a31\u53d6 0.02-1 g \u7d44\u7e54\u4e26\u7528\u9810\u51b7\u7684 PBS \u6e05\u6d17\u3002\u6bcf 1 g \u7d44\u7e54\u52a0\u5165 9 ml \u9810\u51b7\u751f\u7406\u9e7d\u6c34 (0.9% NaCl) \u6216 PBS (0.01 M, pH 7.4)\u3002\u624b\u52d5\u3001\u4f7f\u7528\u6a5f\u68b0\u5747\u8cea\u5668\u6216\u901a\u904e\u8d85\u8072\u6ce2\u5747\u8cea\u3002\u5728 4\u00b0C \u4e0b\u4ee5 1500 x g \u96e2\u5fc3\u52fb\u6f3f 10 \u5206\u9418\u3002\u6536\u96c6\u4e0a\u6e05\u6db2\u4e26\u7acb\u5373\u6e2c\u5b9a\u3002\u4e0a\u6e05\u6db2\u4e2d\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u61c9\u55ae\u7368\u6e2c\u5b9a\u3002<\/li>\n
  5. \u7d30\u80de\u88c2\u89e3\u7269\uff1a\u5c07\u7d30\u80de\u6536\u96c6\u5230\u96e2\u5fc3\u7ba1\u4e2d\u4e26\u7528 PBS \u6d17\u6ecc\u3002 1000\u00d7g \u96e2\u5fc3 10 \u5206\u9418\uff0c\u68c4\u4e0a\u6e05\u3002\u6bcf 1 \u00d7 106 \u500b\u7d30\u80de\u52a0\u5165 300-500 \u03bcl \u751f\u7406\u9e7d\u6c34 (0.9% NaCl) \u6216 PBS (0.01 M, pH 7.4)\uff0c\u7136\u5f8c\u5728\u51b0\u6c34\u6d74\u4e2d\u8d85\u8072\u8655\u7406\u3002\u5728 4 \u00b0C \u4e0b\u4ee5 10,000 \u00d7 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u5c07\u4e0a\u6e05\u6db2\u653e\u5165\u65b0\u7684\u96e2\u5fc3\u7ba1\u4e2d\uff08\u540c\u6642\u7f6e\u65bc\u51b0\u4e0a\uff09\u4e26\u7acb\u5373\u9032\u884c\u5206\u6790\u3002\u4e0a\u6e05\u6db2\u4e2d\u7684\u86cb\u767d\u8cea\u6fc3\u5ea6\u61c9\u55ae\u7368\u6e2c\u5b9a\u3002<\/li>\n
  6. \u553e\u6db2(Saliva) \u5c07\u65b0\u9bae\u553e\u6db2\u6536\u96c6\u5230\u7121\u83cc\u5bb9\u5668\u4e2d\uff0c\u7136\u5f8c\u5728 4\u00b0C \u4e0b\u4ee5 10,000 \u00d7 g \u96e2\u5fc3 10 \u5206\u9418\u3002\u53d6\u4e0a\u6e05\u6db2\uff0c\u7f6e\u65bc\u51b0\u4e0a\uff0c\u7acb\u5373\u6e2c\u5b9a<\/li>\n<\/ol>\n

    \u6a23\u54c1\u4e0d\u61c9\u5305\u542b\u6d17\u6ecc\u5291\uff0c\u5982 SDS\u3001Tween-20\u3001NP-40 \u548c Triton X-100\uff0c\u6216\u9084\u539f\u5291\uff0c\u5982 DTT\u3002\u6a23\u54c1\u4e0d\u61c9\u542b\u6709\u915a\u7d05\u7b49\u67d3\u6599\u3002\u5efa\u8b70\u5728\u9032\u884c\u6b63\u5f0f\u5be6\u9a57\u524d\u5148\u9032\u884c\u521d\u6b65\u5be6\u9a57\uff0c\u78ba\u5b9a\u6a23\u54c1\u7684\u6700\u4f73\u7a00\u91cb\u500d\u6578\u3002<\/p>\n

    \u2022 \u5efa\u8b70\u4f7f\u7528\u65b0\u9bae\u6a23\u54c1\u6216\u6700\u8fd1\u7372\u5f97\u7684\u6a23\u54c1\uff0c\u4ee5\u9632\u6b62\u53ef\u80fd\u5c0e\u81f4\u932f\u8aa4\u7d50\u679c\u7684\u964d\u89e3\u548c\u8b8a\u6027\u3002\r\n\u2022 \u88c2\u89e3\u7de9\u885d\u6db2\u53ef\u80fd\u6703\u5e72\u64fe\u8a66\u5291\u76d2\u3002\u56e0\u6b64\uff0c\u5efa\u8b70\u5c0d\u7d30\u80de\u88c2\u89e3\u7269\u548c\u7d44\u7e54\u52fb\u6f3f\u4f7f\u7528\u6a5f\u68b0\u88c2\u89e3\u65b9\u6cd5\u3002\r\n\r\n\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2\u64cd\u4f5c\u6d41\u7a0b (\u4f9d\u539f\u5ee0\u64cd\u4f5c\u65b9\u5f0f\u70ba\u4e3b)<\/span><\/strong><\/span>\r\n\"\"\r\n<\/span><\/pre>\n
    \u8840\u5c3f\u7d20\u6c2e\uff08Blood Urea Nitrogen , BUN\uff09\u6aa2\u6e2c<\/a> | \u808c\u9150\u6aa2\u6e2c\u8a66\u5291\u76d2(Creatinine Assay Kit)\u00a0<\/a> | UA\u5c3f\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a> | Calcium\u9223\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a> | \u9382\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/p>\n\n\n\n\n\n\n\n\n\n
    \u8ca8\u865f<\/td>\n\u5206\u6790\u54c1\u9805<\/td>\n<\/tr>\n
    abx090684<\/a><\/td>\n\u8840\u5c3f\u7d20\u6c2e\uff08Blood Urea Nitrogen , BUN\uff09\u6aa2\u6e2c<\/a><\/td>\n<\/tr>\n
    abx298944<\/a><\/td>\n\u808c\u9150\u6aa2\u6e2c\u8a66\u5291\u76d2(Creatinine Assay Kit)\u00a0<\/a><\/td>\n<\/tr>\n
    abx298824<\/a><\/td>\nUA\u5c3f\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
    abx298871<\/a><\/td>\nCalcium\u9223\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
    abx298873<\/a><\/td>\n\u9382\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n<\/tr>\n
    abx298918<\/a><\/td>\n\u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2| Nitrite Assay Kit\u8ca8\u865fabx298918<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
     <\/p>\n","protected":false},"excerpt":{"rendered":"
    \u4e9e\u785d\u9178\u9e7d\u6aa2\u6e2c\u8a66\u5291\u76d2| Nitrite Assay Kit\u8ca8\u865fabx298918 Nitrite Assay K […]<\/p>\n","protected":false},"author":3,"featured_media":29387,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5340],"tags":[801],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33157"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33157"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33157\/revisions"}],"predecessor-version":[{"id":46995,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33157\/revisions\/46995"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29387"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33157"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33157"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33157"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}