{"id":33091,"date":"2022-07-07T14:29:04","date_gmt":"2022-07-07T06:29:04","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33091"},"modified":"2022-10-25T20:55:33","modified_gmt":"2022-10-25T12:55:33","slug":"%e5%b1%b1%e7%be%8a%e6%8a%97%e5%85%94-igg-dylight-488%e4%ba%8c%e6%8a%97-goat-anti-rabbit-igg-dylight-488%e8%b2%a8%e8%99%9fa23220","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33091","title":{"rendered":"\u5c71\u7f8a\u6297\u5154 IgG- Dylight 488\u4e8c\u6297 | Goat Anti-Rabbit IgG- Dylight 488\u8ca8\u865fA23220"},"content":{"rendered":"

\u5c71\u7f8a\u6297\u5154 IgG- Dylight 488\u4e8c\u6297 |<\/strong> Goat Anti-Rabbit IgG- Dylight 488\u8ca8\u865f<\/strong>A23220<\/a><\/span><\/p>\n

Goat Anti-Rabbit IgG- Dylight 488<\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

Abbkine \u4e8c\u6297(secondary antibodies)\u5177\u6709\u9176(enzyme)\u3001\u751f\u7269\u7d20(biotin)\u6216\u87a2\u5149\u5718(fluorophore)\u5076\u806f\uff0c\u7528\u65bc\u5404\u7a2e\u57fa\u65bc\u6297\u9ad4\u7684\u61c9\u7528(antibody-based applications)\uff0c\u5305\u62ec\u86cb\u767d\u8cea\u5370\u8de1(Western Blot)\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b78(ImmunoHistoChemistry)\u3001\u514d\u75ab\u87a2\u5149(ImmunoFluorescence)\u3001\u6d41\u5f0f\u7d30\u80de\u8853(Flow Cytometry)\u548c ELISA\u3002\u63d0\u4f9b\u4f86\u81ea\u5c71\u7f8a(goat sources)\u3001\u5154\u5b50(rabbit sources)\u548c\u9a62\u6e90(donkey sources)\u7684\u9ad8\u8cea\u91cf\u4e8c\u6297\u3002\u9084\u63d0\u4f9b\u8840\u6e05\u5438\u9644\u4e8c\u6297(Serum adsorbed secondary antibodies)\uff0c\u5efa\u8b70\u7528\u65bc\u5bcc\u542b\u514d\u75ab\u7403\u86cb\u767d(immunoglobulin-richs)\u7684\u6a23\u54c1\u3002<\/p>\n

DyLight\u87a2\u5149\u67d3\u6599\u662f\u4e00\u7a2e\u65b0\u7684\u67d3\u6599\u5bb6\u65cf\uff0c\u5177\u6709\u66f4\u9ad8\u7684\u4eae\u5ea6\u548c\u5149\u7a69\u5b9a\u6027(photostability\uff09\u3002\u5b83\u5011\u512a\u65bc\u6216\u53ef\u8207\u5176\u4ed6\u516c\u53f8\u6700\u597d\u7684\u87a2\u5149\u67d3\u6599\u76f8\u5ab2\u7f8e\u3002\u4efb\u4f55\u87a2\u5149\u5718-\u6297\u9ad4\u5076\u806f\u7269\u7684\u6aa2\u6e2c\u6c34\u5e73\u53d6\u6c7a\u65bc\u67d3\u6599\u7684\u4eae\u5ea6\u548c\u5149\u7a69\u5b9a\u6027\uff1b\u6297\u9ad4\u6d3b\u6027(antibody activity)\u3001\u7279\u7570\u6027(specificity)\u548c\u4ea4\u53c9\u53cd\u61c9\u6027(cross-reactivity)\uff1b\u548c\u6bcf\u500b\u6297\u9ad4\u7684\u6700\u4f73\u67d3\u6599\u6469\u723e\u6578(optimal moles of dye per antibody)\u3002\u5df2\u7d93\u70ba\u6bcf\u7a2e\u67d3\u6599\u5efa\u7acb\u4e86\u6469\u723e\u98fd\u548c\u5ea6\u66f2\u7dda(molar saturation curve)\u8207\u87a2\u5149\u5f37\u5ea6(fluorescence intensity)\u3001\u6297\u9ad4\u6d3b\u6027(antibody activity)\u3001\u80cc\u666f\u6c34\u5e73(background level)\u548c\/\u6216\u5176\u4ed6\u53c3\u6578\u7684\u95dc\u4fc2\uff0c\u4ee5\u512a\u5316\u6297\u9ad4\u6aa2\u6e2c\u6c34\u5e73\u4e26\u6700\u5c0f\u5316\u80cc\u666f\u3002 DyLight \u87a2\u5149\u67d3\u6599\u5177\u6709\u9ad8\u5ea6\u6c34\u6eb6\u6027(water soluble)\uff0c\u4e26\u4e14\u5728 pH 4 \u5230 pH 9 \u671f\u9593\u4fdd\u6301\u87a2\u5149\u3002<\/p>\n

\u5c71\u7f8a\u6297\u5154 IgG- Dylight 488\u4e8c\u6297\uff0c\u5176\u514d\u75ab\u539f\u70baRabbit IgG whole molecule\u3002Host\u5bbf\u4e3b\u4f86\u6e90\u70baGoat\u3002\u53cd\u61c9\u7269\u7a2e\u70baRabbit\uff0c\u53ef\u61c9\u7528\u65bcFCM, ICC, IF\u3002\u6700\u4f73\u5de5\u4f5c\u7a00\u91cb\u5ea6\u61c9\u7531\u7814\u7a76\u8005\u901a\u904e\u5be6\u9a57\u78ba\u5b9a\u3002\u5efa\u8b70\u7684\u8d77\u59cb\u7a00\u91cb\u5ea6\u5982\u4e0b\uff1aSuggested starting 1:50-1:1000 dilutions for most fluorescent applications\u3002<\/p>\n

\u5c71\u7f8a\u6297\u5154 IgG-HRP\u6a19\u8a8c<\/a>\u00a0|\u00a0\u5c71\u7f8a\u6297\u5154 IgG-Biotin\u6a19\u8a8c<\/a>\u00a0|\u00a0\u5c71\u7f8a\u6297\u5154 IgG- Dylight 488\u4e8c\u6297<\/a>\u00a0|\u00a0\u5c71\u7f8a\u6297\u5154 IgG- Dylight594\u4e8c\u6297<\/a>\u00a0|\u00a0\u5c71\u7f8a\u6297\u5154 IgG- Dylight649\u4e8c\u6297<\/a><\/p>\n

\u00a0\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n

\"\u76f8\u95dc\u7522\u54c1\"<\/a><\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

\"\"<\/p>\n

Fig.1. DyLight fluorescent dyes are a new family of dyes with improved brightness. DyLight 488-antibody conjugates are brighter than Cy2 and FITC conjugates and similar in brightness to Alexa Fluor 488 conjugates. DyLight 549-antibody conjugates shows brighter influoresence than TRITC conjugates. Also, DyLight 594-antibody conjugates are noticeably brighter than Alexa 594 conjugates, and much brighter and more water soluble than Texas Red conjugates.<\/p>\n

\"\"<\/p>\n

Fig.2. DyLight fluorescent dyes show improved photostability as well. DyLight 488 conjugates fade less than FITC and Cy2 conjugates in mounting media indicating that the DyLight 488 molecule is inherently more photostable in epifluorescence microscopes. DyLight 549 conjugates are about as photostable as Alexa 555 conjugates and slightly more photostable than Cy3 conjugates.<\/p>\n

\"\"<\/p>\n

\n
\n
\n
\n
\n
\n

Fig.3. To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488\u00a0nm) laser for DyLight 488, a green (526\u00a0nm) laser for DyLight 550 and 594, and a red (633\u00a0nm) laser for DyLight 633 and 650. The DyLight 680, 755 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700\u00a0nm, 750\u00a0nm and 800\u00a0nm region of the spectrum, respectively.<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nRabbit IgG whole molecule<\/td>\n<\/tr>\n
Host<\/strong><\/td>\nGoat<\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nRabbit<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nFCM, ICC, IF<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nOptimal working dilutions should be determined experimentally by the investigator. Suggested starting 1:50-1:1000 dilutions for most fluorescent applications.<\/td>\n<\/tr>\n
Clonality<\/strong><\/td>\nPolyclonal<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nAffinity purified using solid phase Rabbit IgG (H&L) with finally > 95% purity based on SDS-PAGE<\/td>\n<\/tr>\n
Conjugate<\/strong><\/td>\nDylight 488<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\nDyLight fluorescent dyes are a new family of dyes with improved brightness and photostability). They are better than or comparable to the best fluorescent dyes from other companies). The detection level of any fluorophore-antibody conjugate depends on brightness and photostability of the dye; antibody activity, specificity, and cross-reactivity; and the optimal moles of dye per antibody). A molar saturation curve vs fluorescence intensity, antibody activity, background level, and\/or other parameters has been established for each dye to optimize the level of antibody detection and minimize background). DyLight fluorescent dyes are highly water soluble and remain fluorescent from pH 4 to pH 9).<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at -20\u00b0C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n
<\/div>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/u><\/strong><\/a> \u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/u><\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u54c1\u724c\u4ecb\u7d39\"<\/h2>\n

Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

\u767c\u5c55\u6b77\u7a0b<\/p>\n

Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u3002<\/p>\n

2017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e:<\/p>\n

2018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u3002<\/p>\n

2019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

    \n
  1. \u5168\u5957\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u548c\u5167\u53c3\u6297\u9ad4\uff0c\u4ee5\u53ca\u76f8\u61c9\u7684\u5b9a\u5236\u5076\u806f\u7522\u54c1<\/li>\n
  2. \u5c0f\u5305\u88dd\u7684\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u7279\u7570\u6027\u7684\u6297\u9ad4\u7522\u54c1<\/li>\n
  3. \u6700\u71b1\u9580\u7684\u4e8c\u6297\u7522\u54c1\uff1a\u7528\u65bcIF\u7684IFKine\u7cfb\u5217\u7522\u54c1\uff0c\u4ee5\u53caIP-WB\u7684IPKine\u7cfb\u5217\u7522\u54c1<\/li>\n
  4. \u5168\u5957\u9ad8\u54c1\u8cea\uff0c\u8cb7\u5f97\u8d77\u7684\u86cb\u767d\u8cea\u7d14\u5316\u5de5\u5177\uff08\u586b\u6599\uff0c\u9810\u88dd\u67f1\u548c\u8a66\u5291\u76d2\uff09<\/li>\n
  5. \u66a2\u92b7\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u5305\u542b\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u751f\u9577\u56e0\u6578<\/li>\n
  6. \u66f4\u591a\u7d30\u80de\u529f\u80fd\u5206\u6790\u8a66\u5291\u76d2\uff0cELISA\u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u7d30\u80de\u793a\u8e64\u8a66\u5291<\/li>\n<\/ol>\n

    \u6b64\u5916\uff0c\u6211\u5011\u9084\u5efa\u7acb\u4e86\u6280\u8853\u670d\u52d9\u5e73\u81fa\uff0c\u5305\u62ec\u514d\u75ab\u5b78\u76f8\u95dc\u5be6\u9a57\u670d\u52d9\uff08WB\/IHC\/IF\/IP\uff09\u3001\u86cb\u767d\u5076\u806f\u670d\u52d9\u3001\u86cb\u767d\u8868\u9054\u3001\u751f\u5316\/\u7d30\u80de\u5b78\u5be6\u9a57\u4ee3\u6e2c\u3001Elisa\u5be6\u9a57\u4ee3\u6e2c\u3001\u6297\u9ad4\u88fd\u5099\u7b49\uff0c\u6191\u85c9\u5c08\u696d\u6280\u8853\u3001\u8a2d\u5099\u548c\u670d\u52d9\u614b\u5ea6\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u5be6\u9a57\u89e3\u6c7a\u65b9\u6848\u3002Abbkine\u6642\u523b\u628a\u63e1\u5ba2\u6236\u9700\u6c42\u8b8a\u5316\uff0c\u4e26\u7a4d\u6975\u61c9\u5c0d\u3001\u53ca\u6642\u8abf\u6574\uff0c\u4f7f\u5f97Abbkine\u6210\u70ba\u8a31\u591a\u570b\u969b\u9818\u5148\u7684\u7814\u7a76\u6a5f\u69cb\u548c\u81e8\u5e8a\u8a3a\u65b7\u516c\u53f8\u503c\u5f97\u4fe1\u8cf4\u7684\u53ef\u9760\u4f9b\u61c9\u5546\u3002<\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

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