{"id":33024,"date":"2022-06-30T11:41:39","date_gmt":"2022-06-30T03:41:39","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=33024"},"modified":"2023-03-22T18:17:45","modified_gmt":"2023-03-22T10:17:45","slug":"purkine-his-tag-ni-nta-resin%e6%a8%b9%e8%84%82-%e8%b2%a8%e8%99%9fktb1501","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=33024","title":{"rendered":"PurKine\u2122 His-Tag Ni-NTA Resin\u6a39\u8102 | \u9ad8\u5bb9\u91cf IMAC \u57fa\u8cea(IMAC matrix) \u8ca8\u865fBMR2001"},"content":{"rendered":"

\u9ad8\u54c1\u8cea\u86cb\u767d\u7d14\u5316\u6a39\u8102<\/span>\u00a0 <\/span>|\u00a0PurKine\u2122 His-Tag Ni-NTA Resin\u6a39\u8102<\/span><\/a><\/span><\/strong><\/span><\/p>\n

PurKine\u2122 His-Tag Ni-NTA Resin\u6a39\u8102 | \u8ca8\u865fBMR2001<\/a>\u00a05ML\/25ML<\/span><\/p>\n

PurKine\u2122 His-Tag Ni-NTA Resin-: \u00a050 mg\u00a0 6\u00d7His-tagged protein\/mL\u00a0 \u5e73\u5747\u73e0\u7c92\u5c3a\u5bf8\u70ba45-165 \u00b5m\u00a0 \u00a0<\/span><\/p>\n

\"purkine-2\"<\/p>\n

PurKine\u2122 His-Tag \u7d14\u5316\u7cfb\u7d71\u57fa\u65bc\u5275\u65b0\u7684\u9ad8\u5bb9\u91cf IMAC \u57fa\u8cea(IMAC matrix)\uff0c\u53ef\u65b9\u4fbf\u5730\u5f9e\u7e3d\u88c2\u89e3\u7269(total lysates)\u4e2d\u4e00\u6b65\u7d14\u5316 His-tag \u86cb\u767d(His-tag proteins)\u3002\u6211\u5011\u5c08\u6709\u7684\u96e2\u5b50\u87af\u5408\u5316\u5b78(ion-chelate chemistry)\u78ba\u4fdd\u8207\u5e38\u7528\u7684\u9084\u539f\u5291\uff08\u5982 DTT\uff09\u3001\u87af\u5408\u91d1\u5c6c\u86cb\u767d\u9176\u6291\u88fd\u5291\uff08chelating metalloprotease inhibitors, \u5982 EDTA\uff09\u4ee5\u53ca\u5404\u7a2e\u7de9\u885d\u7269(buffer substances)\u8cea\u548c\u9e7d\u689d\u4ef6\u5177\u6709\u975e\u51e1\u7684\u76f8\u5bb9\u6027\u3002\u8a72\u7522\u54c1\u7d44\u5408\u63d0\u4f9b\u591a\u7a2e\u87af\u5408\u57fa\u5718\uff08chelating group , IDA\u3001NTA \u6216 Super NTA\uff09\u3001\u4ea4\u806f\u74ca\u8102\u7cd6\uff08\u4ea4\u806f 4% \u6216 6% \u74ca\u8102\u7cd6\uff09\u3001\u96e2\u5b50\uff08Nick\u3001Colbat\u3001\u9285\u6216\u975e\u5e36\u96fb\u96e2\u5b50\uff09\u548c\u517c\u5bb9\u6210\u5206\uff0c\u4ee5\u5be6\u73fe\u512a\u5316\u6700\u5927\u86cb\u767d\u8cea\u7522\u91cf\u3001\u7a69\u5b9a\u6027\u548c\u6eb6\u89e3\u5ea6\u7684\u904e\u7a0b\u3002\u6e2c\u8a66\u9084\u8b49\u5be6\uff0c\u5728\u81f3\u5c11\u4e94\u6b21\u91cd\u8907\u4f7f\u7528\u5f8c\u6027\u80fd\u6c92\u6709\u4e0b\u964d\u3002 PurKine\u2122 His-Tag \u7d14\u5316\u6a39\u8102\u4e5f\u53ef\u4f5c\u70ba\u9810\u88dd\u96e2\u5fc3\u67f1(prepacked spin column)\u548c\u8a66\u5291\u76d2(kit)\u5f62\u5f0f\u63d0\u4f9b\u3002PurKine\u2122 His-Tag Ni-NTA Resin \u5728\u5404\u7a2e\u898f\u6a21\u7684\u91cd\u529b\u67f1(gravity column)\u7a0b\u5e8f\u4e2d\u6709\u6548\u7d14\u5316\u9ad8\u6c34\u5e73\u904e\u8868\u9054\u7684 His-tagged \u878d\u5408\u86cb\u767d\u3002\u8a72\u6a39\u8102(Resin)\u7531 90\u03bcm \u9ad8\u5ea6\u4ea4\u806f\u7684 4% \u74ca\u8102\u7cd6\u73e0(cross-linked 4% agarose)\u7d44\u6210\uff0c\u8207\u785d\u9178\u4e09\u4e59\u9178 (Nitilotriacetic acid , NTA) \u5076\u806f\u3002\u7136\u5f8c\u87af\u5408\u57fa\u5718\u5e36\u6709\u93b3\u96e2\u5b50 (nickel ions , Ni2+)\u3002\u6e2c\u8a66\u8b49\u5be6\u5176\u6027\u80fd\u7b49\u65bc\u6216\u8d85\u904e\u5176\u4ed6\u4f9b\u61c9\u5546\u7684\u6d41\u884c Ni-NTA \u6a39\u8102\uff0c\u4e26\u4e14\u5728\u81f3\u5c11\u4e94\u6b21\u91cd\u8907\u4f7f\u7528\u5f8c\u6027\u80fd\u6c92\u6709\u4e0b\u964d\u3002<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nPurKine\u2122 His-Tag Ni-NTA Resin<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nPurKine\u2122 His-Tag Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins in gravity column procedures at a variety of scales. The Resin consists of 90\u03bcm beads of highly cross-linked 4% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni2+). Tests confirm that performance equals or exceeds popular Ni-NTA resins from other suppliers, and no decrease in performance occurs after at least five repeated uses.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution with 50% slurry in 20% ethanol.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 High capacity\u2014More than 50 mg of 6xHis-tagged protein per milliliter of resin.
\n\u2022 Versatile\u2014For purification under native or denaturing conditions.
\n\u2022 Cost-effective\u2014No performance decrease after at least five repeated uses of the same batch of resin.
\n\u2022 Flexible\u2014available in multiple formats including bulk resin, spin columns and complete kits.<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nLiquid in PBS buffer containing 20% ethanol.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStable for one year at 2-8\u00b0C from date of shipment. Do not freeze.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nBlue ice<\/td>\n<\/tr>\n
Background<\/strong><\/td>\nPurKine\u2122 His-Tag Purification system is based on innovative high-capacity IMAC matrix for convenient single-step purification of His-tag proteins from total lysates. Our propriety ion-chelate chemistry ensures extraordinary compatibility with commonly used reducing agents such as DTT, chelating metalloprotease inhibitors such as EDTA, and a wide range of buffer substances and salt conditions. The portfolio provides wide choice of chelating group (IDA, NTA or Super NTA), cross-linked agarose (crosslinked 4% or 6% agarose), ions (Nick, Colbat, Copper, or non ion charged) and compatible ingredients to allow optimization of process for maximum protein yield, stability and solubility. Tests also confirm that no decrease in performance occurs after at least five repeated uses. The PurKine\u2122 His-Tag Purification resin is also available as prepacked spin column and kit formats.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nNi-NTA; His resin; Ni-NTA resin; His tag resin<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

BRAND\/AGENCY\/\u53f0\u7063\u4ee3\u7406\u5546<\/span>\/\u00a0<\/span>Abbkine\u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/span><\/strong><\/h2>\n

\"abbkine\u5546\u54c1\u63d2\u5716\"\"\u539f\u5ee0\u9023\u7d5001\"<\/a><\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716<\/p>\n

https:\/\/www.abbkine.com\/<\/p>\n

Abbkine Scientific Co., Ltd\u7531\u4e00\u7fa4\u5fd7\u540c\u9053\u5408\u7684\u79d1\u5b78\u5bb6\u548c\u884c\u92b7\u5c08\u5bb6\uff0c\u57282012\u5e74\u6210\u7acb\u65bc\u7f8e\u570b\u52a0\u5229\u798f\u5c3c\u4e9e\u5dde\uff0c\u96a8\u8457\u4e9e\u6d32\u5e02\u5834\u7684\u8fc5\u731b\u589e\u9577\uff0cAbbkine\u5c07\u7e3d\u90e8\u642c\u9077\u81f3\u4e2d\u570b\u3002\u7d50\u5408\u4e86Abbkine\u7f8e\u570b\u6280\u8853\u548c\u4e2d\u570b\u88fd\u9020\u5169\u65b9\u9762\u7684\u5de8\u5927\u512a\u52e2\uff0cAbbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\uff0c\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002<\/p>\n

\u767c\u5c55\u6b77\u7a0b<\/span><\/p>\n

Abbkine\u81f4\u529b\u65bc\u63d0\u4f9b\u5275\u65b0\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u5be6\u9a57\u8a66\u5291\u76d2\u3001\u86cb\u767d\u8cea\u3001\u6297\u9ad4\u4ee5\u53ca\u5176\u5b83\u7814\u7a76\u5de5\u5177\u4ee5\u671f\u6210\u70ba\u751f\u547d\u79d1\u5b78\u7814\u7a76\u767c\u5c55\uff0c\u85e5\u7269\u7814\u767c\u7b49\u9818\u57df\u7684\u95dc\u9375\u63a8\u52d5\u8005\u3002Abbkine\u4ee5\u7279\u8272\u7684\u86cb\u767d\u8cea\u751f\u7522\u8207\u6aa2\u6e2c\u8a66\u5291\u8d77\u5bb6\uff0c\u81f4\u529b\u65bc\u5275\u65b0\u548c\u7814\u767c\u5404\u985e\u6297\u9ad4\u3001\u751f\u5316\u8a66\u5291\u3001\u86cb\u767d\u8cea\u548c\u5be6\u9a57\u8a66\u5291\u76d2\uff0c\u4ee5\u671f\u671b\u70ba\u5168\u7403\u79d1\u7814\u5de5\u4f5c\u8005\u63d0\u4f9b\u9769\u65b0\u7684\u89e3\u6c7a\u65b9\u6848\u3002\u6211\u5011\u5f88\u81ea\u8c6a\u5730\u70ba\u5ba2\u6236\u63d0\u4f9b\u5982\u4e0b\u7279\u8272\u7522\u54c1\u7dda\uff0c\u4e26\u6703\u4e00\u5982\u65e2\u5f80\u5730\u958b\u767c\u65b0\u578b\u7684\u3001\u9ad8\u54c1\u8cea\u7684\u3001\u8cb7\u5f97\u8d77\u7684\u751f\u547d\u79d1\u5b78\u7814\u7a76\u548c\u8a3a\u65b7\u8a66\u5291\u7522\u54c1\u30022017\u5e74\u6aa2\u6e2c\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u7684ELISA\u8a66\u5291\u76d2\u61c9\u904b\u800c\u751f: \u6839\u64da\u5e02\u5834\u9700\u6c42\uff0c\u958b\u767cEliKine\u2122\u7cfb\u5217ELISA\u8a66\u5291\u76d2\uff0c\u6db5\u84cb\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u6fc0\u7d20\u71b1\u9580\u6307\u6a19\uff0c\u5982\u767d\u4ecb\u7d20\uff08IL\uff09\u3001\u816b\u7624\u58de\u6b7b\u56e0\u6578\uff08TNF\uff09\u3001\u5e72\u64fe\u7d20\uff08IFN\uff09\u7b49\u660e\u661f\u7d1a\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\u5168\u7403\u767c\u552e\u30022018\u5e74\u6191\u85c9\u5148\u9032\u7684\u86cb\u767d\u8cea\u5076\u806f\u6280\u8853\u548c\u56b4\u683c\u7684\u54c1\u8cea\u63a7\u5236\uff0c\u6211\u5011\u63d0\u4f9bLinKine\u2122\u5076\u806f\u6a19\u8a18\u8a66\u5291\u76d2\uff0c\u5728\u6297\u9ad4\u3001\u86cb\u767d\u8cea\u3001\u591a\u80bd\u6216\u8005\u5176\u5b83\u751f\u7269\u5206\u5b50\u4e0a\u53ef\u76f4\u63a5\u6a19\u8a18HRP\uff0cBiotin\uff0cFITC\uff0cCy3\uff0cAbFluor\u2122\u4ee5\u6eff\u8db3\u79d1\u7814\u61c9\u7528\uff0c\u85e5\u7269\u7814\u767c\u4ee5\u53ca\u8a3a\u65b7\u8a66\u5291\u76d2\u958b\u767c\u7684\u9700\u6c42\u30022019\u5e74\u751f\u5316\u3001\u7d30\u80de\u985e\u7522\u54c1\u7dda\u9010\u6b65\u64f4\u5145\u642d\u5efa\u7d30\u80de\u7522\u54c1\u7814\u767c\u5e73\u81fa\uff0c\u7d30\u80de\u72c0\u614b\u6aa2\u6e2c\u3001\u7d30\u80de\u67d3\u8272\u3001\u7d30\u80de\u63d0\u53d6\u3001\u751f\u5316\u8a66\u5291\u76d2\u7522\u54c1\u5f97\u5230\u8d8a\u4f86\u8d8a\u591a\u5168\u7403\u5ba2\u6236\u7684\u8a8d\u53ef\u3002<\/p>\n

\"\"<\/p>\n

    \n
  1. \u5168\u5957\u8868\u4f4d\u6a19\u7c64\u6297\u9ad4\u548c\u5167\u53c3\u6297\u9ad4\uff0c\u4ee5\u53ca\u76f8\u61c9\u7684\u5b9a\u5236\u5076\u806f\u7522\u54c1<\/li>\n
  2. \u5c0f\u5305\u88dd\u7684\u78f7\u9178\u5316\u548c\u7532\u57fa\u5316\u7279\u7570\u6027\u7684\u6297\u9ad4\u7522\u54c1<\/li>\n
  3. \u6700\u71b1\u9580\u7684\u4e8c\u6297\u7522\u54c1\uff1a\u7528\u65bcIF\u7684IFKine\u7cfb\u5217\u7522\u54c1\uff0c\u4ee5\u53caIP-WB\u7684IPKine\u7cfb\u5217\u7522\u54c1<\/li>\n
  4. \u5168\u5957\u9ad8\u54c1\u8cea\uff0c\u8cb7\u5f97\u8d77\u7684\u86cb\u767d\u8cea\u7d14\u5316\u5de5\u5177\uff08\u586b\u6599\uff0c\u9810\u88dd\u67f1\u548c\u8a66\u5291\u76d2\uff09<\/li>\n
  5. \u66a2\u92b7\u7684\u91cd\u7d44\u86cb\u767d\uff0c\u5305\u542b\u5404\u985e\u7d30\u80de\u56e0\u6578\u548c\u751f\u9577\u56e0\u6578<\/li>\n
  6. \u66f4\u591a\u7d30\u80de\u529f\u80fd\u5206\u6790\u8a66\u5291\u76d2\uff0cELISA\u8a66\u5291\u76d2\uff0c\u4ee5\u53ca\u7d30\u80de\u793a\u8e64\u8a66\u5291<\/li>\n<\/ol>\n

    \u6b64\u5916\uff0c\u6211\u5011\u9084\u5efa\u7acb\u4e86\u6280\u8853\u670d\u52d9\u5e73\u81fa\uff0c\u5305\u62ec\u514d\u75ab\u5b78\u76f8\u95dc\u5be6\u9a57\u670d\u52d9\uff08WB\/IHC\/IF\/IP\uff09\u3001\u86cb\u767d\u5076\u806f\u670d\u52d9\u3001\u86cb\u767d\u8868\u9054\u3001\u751f\u5316\/\u7d30\u80de\u5b78\u5be6\u9a57\u4ee3\u6e2c\u3001Elisa\u5be6\u9a57\u4ee3\u6e2c\u3001\u6297\u9ad4\u88fd\u5099\u7b49\uff0c\u6191\u85c9\u5c08\u696d\u6280\u8853\u3001\u8a2d\u5099\u548c\u670d\u52d9\u614b\u5ea6\uff0c\u70ba\u5ba2\u6236\u63d0\u4f9b\u4e00\u7ad9\u5f0f\u5be6\u9a57\u89e3\u6c7a\u65b9\u6848\u3002Abbkine\u6642\u523b\u628a\u63e1\u5ba2\u6236\u9700\u6c42\u8b8a\u5316\uff0c\u4e26\u7a4d\u6975\u61c9\u5c0d\u3001\u53ca\u6642\u8abf\u6574\uff0c\u4f7f\u5f97Abbkine\u6210\u70ba\u8a31\u591a\u570b\u969b\u9818\u5148\u7684\u7814\u7a76\u6a5f\u69cb\u548c\u81e8\u5e8a\u8a3a\u65b7\u516c\u53f8\u503c\u5f97\u4fe1\u8cf4\u7684\u53ef\u9760\u4f9b\u61c9\u5546\u3002<\/p>\n

    \u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09\u00a0|\u00a0\u8ca8\u865fKTA3030<\/a>\u00a0<\/span><\/strong><\/p>\n

    Cell Senescence Kit (\u03b2-Galactosidase Assay)<\/span><\/p>\n

    \u7d30\u80de\u8870\u8001(Cellular senescence)\u662f\u6b63\u5e38\u7d30\u80de\u505c\u6b62\u5206\u88c2(cell divide)\u7684\u73fe\u8c61\u3002\u8870\u8001\u7684\u7d30\u80de(senescent cells)\u4e0d\u518d\u80fd\u5920\u8907\u88fd(replicate)\uff0c\u4f46\u5b83\u5011\u4ecd\u4fdd\u6301\u4ee3\u8b1d\u6d3b\u6027(metabolically active)\uff0c\u4f46\u56e0\u963b\u6eef\u65bcG1\u671f\uff0c\u5931\u53bb\u4e86\u5c0d\u6709\u7d72\u5206\u88c2\u539f\u7684\u53cd\u61c9\u80fd\u529b\u548c\u5408\u6210DNA\u7684\u80fd\u529b\uff0c\u4e0d\u80fd\u9032\u5165S\u671f\u3002\u4e26\u4e14\u8207\u8870\u8001\u76f8\u95dc\u7684\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u6d3b\u6027\u67d3\u8272(senescence-associated beta-galactosidase activity)\u5448\u967d\u6027\uff0c\u9019\u88ab\u8a8d\u70ba\u662f\u7d30\u80de\u8870\u8001\u7684\u751f\u7269\u6a19\u8a18(biomarker)\u3002\u7d30\u80de\u7684\u8870\u8001(Cellular senescence)\u53ef\u4ee5\u901a\u904e\u6fc0\u6d3b\u764c\u57fa\u56e0(oncogenes)\u548c\u7d30\u80de-\u7d30\u80de\u878d\u5408(cell-cell fusion)\u4f86\u5be6\u73fe\uff0cDNA\u5c0d\u6d3b\u6027\u6c27\uff08reactive oxygen species , ROS\uff09\u5347\u9ad8\u7684\u53cd\u61c9\u6703\u5c0e\u81f4DNA\u640d\u50b7(DNA damage)\uff0c\u800c\u4e0d\u662f\u50c5\u50c5\u4f9d\u8cf4\u65bc\u7d30\u80de\u5206\u88c2\u7684\u6578\u76ee\u3002\u5728\u6b63\u5e38\u8870\u8001\u904e\u7a0b(normal aging)\u4e2d\uff0c\u7d44\u7e54\u4e2d\u7684\u8870\u8001\u7d30\u80de\u6578\u91cf\u986f\u8457\u589e\u52a0\u3002<\/p>\n

    \"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

    \u7d30\u80de\u662f\u751f\u7269\u7d50\u69cb(biological structure)\u548c\u529f\u80fd(function)\u7684\u57fa\u672c\u55ae\u4f4d\uff0c\u4e5f\u662f\u751f\u7269\u8870\u8001(biological aging)\u7684\u57fa\u672c\u55ae\u4f4d\u3002\u7d30\u80de\u8870\u8001(Cellular senescence)\u5728\u5f62\u614b\u4e0a\u8868\u73fe\u70ba\u7d30\u80de\u7d50\u69cb(cell structure)\u7684\u9000\u5316\uff0c\u4f8b\u5982\u6838\u819c\u51f9\u9677(nuclear membrane depression)\uff0c\u6700\u7d42\u5c0e\u81f4\u6838\u819c\u584c\u9677(nuclear membrane collapse)\uff0c\u67d3\u8272\u8cea\u7d50\u69cb(chromatin structure)\u6539\u8b8a\uff0c\u8d85\u4e8c\u500d\u9ad4(hyperdiploid)\u548c\u7570\u5e38\u591a\u500d\u9ad4\u7d30\u80de(polyploid cells)\u6578\u91cf\u589e\u52a0\u3002\u7d30\u80de\u819c\u7684\u8106\u6027(cell membrane fragility)\u589e\u52a0\u3002\u901a\u900f\u6027(permeability)\u964d\u4f4e\uff0c\u819c\u53d7\u9ad4(membrane receptors)\u7684\u985e\u578b\u548c\u6578\u91cf\u4ee5\u53ca\u5c0d\u914d\u9ad4(ligands)\u7684\u654f\u611f\u6027\u767c\u751f\u8b8a\u5316\uff1b\u8102\u8910\u7d20(lipofuscin)\u5728\u7d30\u80de\u4e2d\u7a4d\u7d2f\uff0c\u8a31\u591a\u7d30\u80de\u5668\u548c\u7d30\u80de\u5167\u7d50\u69cb\u767c\u751f\u8b8a\u6027\u3002\u7d30\u80de\u8870\u8001(Cell senescence)\u5728\u751f\u7406\u4e0a\u8868\u73fe(physiologically manifested)\u70ba\u529f\u80fd\u4e0b\u964d\u548c\u65b0\u9673\u4ee3\u8b1d\u4f4e\u4e0b\uff0c\u4f8b\u5982\u7d30\u80de\u9031\u671f\u505c\u6eef(cell cycle arrest)\uff0c\u7d30\u80de\u8907\u88fd\u80fd\u529b (cell replication ability) \u55aa\u5931\uff0c\u5c0d\u4fc3\u6709\u7d72\u5206\u88c2\u523a\u6fc0(mitogenic stimulation)\u7684\u53cd\u61c9\u6027\u6e1b\u5f31\u4ee5\u53ca\u5c0d\u4fc3\u51cb\u4ea1\u56e0\u5b50(pro-apoptotic factors)\u7684\u53cd\u61c9\u6027\u6539\u8b8a\u3002\u7d30\u80de\u5167\u9176\u6d3b\u6027\u4e2d\u5fc3\u88ab\u6c27\u5316\uff0c\u9176\u6d3b\u6027(Enzyme activity)\u964d\u4f4e\uff0c\u86cb\u767d\u8cea\u5408\u6210(protein synthesis)\u964d\u4f4e\u7b49\u3002\u8870\u8001\u7d30\u80de(senescent cells)\u4e0d\u518d\u8907\u5236\uff0c\u4f46\u5b83\u5011\u4ecd\u4fdd\u6301\u4ee3\u8b1d\u6d3b\u6027(metabolically active)\uff0c\u4e26\u4e14\u8870\u8001\u76f8\u95dc\u7684\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\u6d3b\u6027(beta-galactosidase activity)\u5448\u967d\u6027\uff0c\u9019\u88ab\u8a8d\u70ba\u662f\u7d30\u80de\u8870\u8001\u751f\u7269\u6a19\u8a18(biomarker of cellular senescence)\u3002<\/p>\n

    \"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

    Abbkine\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08Senescence \u03b2-Galactosidase Staining Kit, KTA3030\uff09\u63d0\u4f9b\u4e86\u57fa\u65bc\u8870\u8001\u904e\u7a0b\u4e2dpH\u503c\u70ba6\u7684\u8870\u8001\u76f8\u95dc\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff08SA-\u03b2-Gal\uff09\u6d3b\u6027\u4e0a\u8abf\u7684\u8a66\u5291\uff0c\u7528\u65bc\u6aa2\u6e2c\u8870\u8001\u7d30\u80de(senescent cells)\u6216\u7d44\u7e54(tissues)\u3002\u7d30\u80de\u6216\u7d44\u7e54\u7684\u8001\u5316\u53ef\u4ee5\u5728\u666e\u901a\u7684\u5149\u5b78\u986f\u5fae\u93e1\u4e0b\u89c0\u5bdf\u3002 SA-\u03b2-Gal\u50c5\u5b58\u5728\u65bc\u8870\u8001\u7d30\u80de(senescent cells)\u4e2d\uff0c\u5728\u8870\u8001\u524d\uff0c\u975c\u6b62\uff0c\u816b\u7624\u6216\u6c38\u751f\u7d30\u80de\u4e2d\u7121\u6cd5\u6aa2\u6e2c\u5230Senescence \u03b2-Galactosidase Staining Kit\u00a0 provide reagents for detecting the senescent cells or tissues based on the up-regulation of senescence-associated \u03b2-galactosidase (SA-\u03b2-Gal) activity at pH 6 during aging. The aging of cells or tissues can be observed under a common optical microscope. SA-\u03b2-Gal is present only in senescent cells and is undetectable in presenescent, quiescent, tumor or immortal cells<\/p>\n

    \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/p>\n

    \u7d30\u80de\u589e\u6b96&\u6bd2\u7406&\u8870\u8001 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
    \u8ca8\u865fKTA1001<\/a><\/span><\/td>\n\u6d3b&\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\n

    \u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/span><\/p>\n

    \u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/span><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/span><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n1000\u500b\u53cd\u61c9<\/span><\/td>\n\u5438\u6536\u5149450nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTA2030<\/a><\/span><\/td>\nEdU Image\u7d30\u80de\u589e\u6b96\u8a66\u5291\u76d2<\/a>\u00a0(BrdU\u66ff\u4ee3\u6cd5)<\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1110<\/a><\/span><\/td>\n\uff08LDH\uff09\u4e73\u9178\u812b\u6c2b\u9176\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

    \u03bbmax= 450 nm<\/span><\/p>\n

    1- 20 U\/mL of LDH<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/span><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em=535\/615 nm.<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/span><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    \u00a0OD 565 nm<\/span><\/p>\n

    10,00-100,0000 cells per well<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/span><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/span><\/td>\n50ug<\/span><\/td>\n\n

    \u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/span><\/p>\n

    \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u00a0\u7d30\u80de\u51cb\u4ea1&\u7d30\u80de\u67d3\u8272 (8)<\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
    \n

    \u8ca8\u865f\u00a0KTA2010<\/a><\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/span><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9<\/span><\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTA4001<\/a><\/span><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\n

    \uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/span><\/p>\n

    JC-1 Stain<\/span><\/p>\n

    CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/span><\/td>\nDAPI Staining Solution<\/a><\/span><\/td>\n10MG<\/span><\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/span><\/td>\n<\/tr>\n
    \u00a0KTD102-EN<\/a><\/span><\/td>\n\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5)<\/a><\/span><\/td>\n40\u500b\u5206\u6790\/20\u500b\u5206\u6790<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/span><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 500\/520 nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/span><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/span><\/td>\nEx\/Em = 484\/501 nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/span><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/span><\/td>\n100\u500b\u53cd\u61c9<\/span><\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    DNA Damege\u640d\u50b7\/\u7d30\u80deStress (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/strong><\/td>\n\u53cd\u61c9<\/span><\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/strong><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1050<\/a><\/span><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 532 nm<\/span><\/p>\n

    1-100 \u00b5M\/L<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTB1910<\/a><\/span><\/td>\n\u6d3b\u6027\u6c27\uff08ROS\uff09\u6aa2\u6e2c\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/span><\/td>\nDCFH-DA (10 mM)<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1400<\/a><\/span><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    colorimetric (540 nm)<\/span><\/p>\n

    \u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/span><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/span><\/td>\n<\/tr>\n
    \u00a0\u8ca8\u865f<\/u><\/strong>\u00a0KTB1091<\/a><\/span><\/td>\n\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\u5438\u6536\u5149 520nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1041<\/a><\/span><\/td>\nCheKine\u2122 \u904e\u6c27\u5316\u6c2b (H2O2) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    480\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    OD 580 nm<\/span><\/p>\n

    : 1 \u00b5M-100 \u00b5M<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1210<\/a><\/span><\/td>\nCheKine\u2122 \u8d85\u6c27\u9670\u96e2\u5b50\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    absorption peak at 540nm<\/span><\/p>\n

    0.0125-0.5 mg\/mL<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1020<\/a><\/span><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD565 nm<\/span><\/p>\n

    96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u6297\u6c27\u5316\u5206\u6790\u8a66\u5291 (8)<\/span><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1030<\/a><\/span><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		200-3.13 U\/ml<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/span><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 540 nm<\/span><\/p>\n

    2-75 \u00b5M<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/span><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTCA\u5206\u6790<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    480\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    593 nm<\/span><\/p>\n

    \u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/span><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		240nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/span><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		412 nm \u00a02-200 \u00b5g\/mL<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB3010<\/a><\/span><\/td>\nCheKine\u2122 \u82b1\u9752\u7d20\u9084\u539f\u9176\uff08ANR\uff09\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\nabsorption at 340 nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1530<\/a><\/span><\/td>\n\u9ec3\u916e\u985e\u5316\u5408\u7269\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    absorption peak at 502 nm<\/span><\/p>\n

    0.156-10 mg\/g<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1140<\/a><\/span><\/td>\n\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    \u00a0absorption peak at 525 nm<\/span><\/p>\n

    \u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u81bd\u56fa\u9187\u4ee3\u8b1d\/\u4ee3\u8b1d\u8a66\u9a57 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1510<\/a><\/span><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    \u00a0\u5438\u6536\u5149505 nm<\/span><\/p>\n

    Standard: 5 \u03bcmol\/mL.<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/span><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

    \u00a0\u5438\u5149\u503c630nm<\/span><\/p>\n

    4- 300 mg\/dL)<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1340<\/a><\/span><\/td>\nCheKine\u2122 \u809d\u91a3\/\u7cd6\u539f<\/u><\/strong>\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/span><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    500nm Standard: 5 \u03bcmol\/mL<\/span><\/p>\n

    \u00a0<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB2250<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u9ad8\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (HDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    Absorption peak at 500nm<\/span><\/p>\n

    Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.156-10 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB2260<\/a><\/span><\/td>\nCheKine\u2122 \u5fae\u91cf\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d\u81bd\u56fa\u9187 (LDL-C) \u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		\n

    Absorption peak at 500nm<\/span><\/p>\n

    Detection range: 0.078-5 mmol\/L(The Detection range corresponds to the standard, and the actual content of sample is 0.117-7.5 mmol\/L\uff09<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1390<\/a><\/span><\/td>\nCheKine\u2122 \u6fb1\u7c89\u5206\u652f\u9176 (SBE) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		absorption at 660 nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 450 nm<\/span><\/p>\n

    \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u751f\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2 (8)<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/span><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n\u5438\u6536\u5149510 nm<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1542<\/a><\/span><\/td>\nTannase(TAN) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

    absorbance at 270 nm<\/span><\/p>\n

    0.3125-20 \u03bcmoL\/mL<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1541<\/a><\/span><\/td>\nCheKine\u2122 \u55ae\u5be7\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | CheKine\u2122 Tannin Colorimetric Assay Kit<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\n\n

    absorbance at 760 nm<\/span><\/p>\n

    0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1290<\/a><\/span><\/td>\nCheKine\u2122 \u70cf\u982d\u9178\u9176 (ACO) \u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9\/<\/span><\/td>\nabsorption at 340 nm.<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTB1560<\/a><\/span><\/td>\n\u9187\u91af\u57fa\u8f49\u79fb\u9176\uff08AAT\uff09\u6d3b\u6027\u6bd4\u8272\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

    48\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96\u500b\u53cd\u61c9<\/span><\/td>\n

    		absorption at 412 nm.<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/span><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/span><\/td>\n96\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 450 nm<\/span><\/p>\n

    \u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865fKTB1380<\/a><\/span><\/td>\n\u03b2-\u6fb1\u7c89\u9176\u6d3b\u6027\u6bd4\u8272\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

    absorption peak at 540 nm<\/span><\/p>\n

    0.0156-1 mg\/mL<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTB1310<\/a><\/span><\/td>\n\u8461\u8404\u7cd6\u6c27\u5316\u9176\u6d3b\u6027\u6e2c\u5b9a<\/a><\/span><\/td>\n48\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 580 \u4e0b\u6e2c\u91cf<\/span><\/p>\n

    \u5075\u6e2c\u7bc4\u570d:\u00a00.05 – 2 U\/L<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u767c\u708e\u53cd\u61c9ELISA\u5206\u6790<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/strong><\/td>\n\u7522\u54c1\u54c1\u9805<\/strong><\/td>\n\u53cd\u61c9<\/strong><\/td>\n\u6e2c\u91cf\u5100\u5668<\/strong><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/span><\/td>\nIL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		\n

    450 nm<\/span><\/p>\n

    \u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/span><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		\n

    450 nm<\/span><\/p>\n

    7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/span><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		\n

    450 nm<\/span><\/p>\n

    \u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/span><\/td>\n<\/tr>\n

    KET7013<\/span><\/td>\nIL-22 ELISA\u8a66\u5291\u76d2<\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KET7003<\/a><\/span><\/td>\nIFN-\u03b3\uff08\u5e72\u64fe\u7d20-\u03b3\uff09ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		31.25 pg\/ml- 2000 pg\/ml<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKET6019<\/a><\/span><\/td>\nIL-10 ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    \u00a0<\/span><\/td>\n

    		2.35 pg\/ml-150 pg\/ml<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKET7002<\/span><\/td>\nGM-CSF ELISA\u8a66\u5291\u76d2<\/span><\/td>\n96\u500b\u53cd\u61c9\/<\/span><\/td>\n7.8 pg\/mL-500 pg\/mL<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/span><\/td>\n\u767d\u4ecb\u7d20-1\u03b2ELISA\u8a66\u5291\u76d2<\/a><\/span><\/td>\n\n

    96\u500b\u53cd\u61c9\/<\/span><\/p>\n

    96 X5\u76e4<\/span><\/td>\n

    		\n

    Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/span><\/p>\n

    \u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u91cd\u7d44\u86cb\u767d&\u55ae\u591a\u682a\u6297\u9ad4<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u6e2c\u91cf\u5100\u5668<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>PRP100273<\/a><\/span><\/td>\nHuman IL-4 protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n20ug<\/span><\/td>\nED50 for this effect is 0.05-0.2 ng\/mL. The specific activity of Recombinant Human IL-4 is approximately 3.2 \u00d7 10E4 IU\/\u03bcg.<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>PRP100159<\/a><\/span><\/td>\nHuman EGF protein\u91cd\u7d44\u86cb\u767d<\/u><\/strong><\/a><\/span><\/td>\n100ug<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>PRP1012<\/a><\/span><\/td>\nHuman IL-6 protein\u91cd\u7d44\u86cb\u767d\u00a0<\/u><\/strong><\/a><\/span><\/td>\n5ug<\/span><\/td>\nhuman IL-6 (NP_000591.1) (Val 30-Met 212)\/ E. coli<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>ABP52783<\/a><\/span><\/td>\nGAPDH Polyclonal Antibody<\/a><\/span><\/td>\n30ul<\/span><\/td>\nHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fA21010<\/span><\/td>\nHRP, Goat Anti-Mouse IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fA21020<\/span><\/td>\nHRP, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fA23220<\/span><\/td>\nDylight 488, Goat Anti-Rabbit IgG<\/span><\/td>\n100ul<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u6297\u9ad4\u86cb\u767d\u7d14\u5316&\u5176\u5b83<\/span><\/strong><\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTP2070<\/a><\/span><\/td>\n\u6297\u9ad4\u7d14\u5316\u8a66\u5291\u76d2\uff08\u86cb\u767d A\/G\uff09<\/a><\/span><\/td>\n1ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u00a0\u8ca8\u865fBMR2001<\/a><\/span><\/td>\nHis-Tag Ni-NTA Resin\u6a39\u8102<\/a><\/span><\/td>\n5ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>\u00a0KTD105-EN<\/a><\/span><\/td>\nIP\/Co-IP Toolkit (Agarose)\u00a0<\/a><\/span><\/td>\n20\u500b\u53cd\u61c9<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865fKTD3001<\/a><\/span><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/span><\/td>\n500\u500b\u53cd\u61c9<\/span><\/td>\n\n

    OD 562 nm<\/span><\/p>\n

    \u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/span><\/p>\n

    50 to 1000 \u00b5g\/ml.<\/span><\/td>\n<\/tr>\n

    \u8ca8\u865f<\/u><\/strong>KTP2140<\/a><\/span><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u6a39\u8102(\u5957\u7d44)<\/a><\/span><\/td>\n1ml\u00d75<\/span><\/td>\nMore than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/span><\/td>\n<\/tr>\n
    BMD0062<\/span><\/td>\nHoechst 33342<\/span><\/td>\n10MG<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    BMU104-EN<\/span><\/td>\n\u589e\u5f37\u578bAntifade\u5c01\u7247\u5291<\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n
    \u8ca8\u865f<\/u><\/strong>BMS0050<\/a><\/span><\/td>\n\u6b63\u5e38\u5c71\u7f8a\u8840\u6e05<\/a><\/span><\/td>\n10ml<\/span><\/td>\n\u00a0<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

    \u7d30\u80de\u57f9\u990a\u76f8\u95dc<\/span><\/strong><\/span><\/p>\n\n\n\n\n
    \u7522\u54c1\u8ca8\u865f<\/span><\/td>\n\u7522\u54c1\u54c1\u9805<\/span><\/td>\n\u53cd\u61c9<\/span><\/td>\n\u7279\u9ede<\/td>\n<\/tr>\n
    BMU108-EN<\/strong><\/a><\/span><\/td>\nSuperKine\u2122 \u7121\u8840\u6e05\/\u7121\u86cb\u767d\u7d30\u80de\u51b7\u51cd\u57f9\u990a\u57fa<\/a><\/span><\/td>\n100ML\u00a0<\/span><\/td>\n\u6210\u5206\u6e05\u6670\uff0c\u7121\u8840\u6e05\/\u7121\u86cb\u767d\uff0c\u907f\u514d\u75c5\u6bd2\u3001\u7d30\u83cc\u3001\u652f\u539f\u9ad4\u7b49\u6c61\u67d3<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u9ad8\u54c1\u8cea\u86cb\u767d\u7d14\u5316\u6a39\u8102\u00a0 |\u00a0PurKine\u2122 His-Tag Ni-NTA Resin\u6a39\u8102 PurKine\u2122 H […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,1],"tags":[823],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33024"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=33024"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33024\/revisions"}],"predecessor-version":[{"id":40403,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/33024\/revisions\/40403"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=33024"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=33024"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=33024"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}