{"id":32998,"date":"2022-06-28T13:17:42","date_gmt":"2022-06-28T05:17:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32998"},"modified":"2022-07-19T11:09:39","modified_gmt":"2022-07-19T03:09:39","slug":"gapdh-polyclonal-antibody-abbkine%e8%b2%a8%e8%99%9fabp52783","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32998","title":{"rendered":"GAPDH Polyclonal Antibody | Abbkine\u8ca8\u865fABP52783"},"content":{"rendered":"

GAPDH Polyclonal Antibody | <\/strong>Abbkine\u8ca8\u865f<\/strong>ABP52783<\/a><\/span><\/p>\n

Rabbit anti-GAPDH Polyclonal Antibody<\/p>\n

\u8fa8\u8996\u7269\u7a2eHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002<\/p>\n

\"Fig.<\/p>\n

GAPDH \u7de8\u78bc 3-\u78f7\u9178\u7518\u6cb9\u919b\u812b\u6c2b\u9176\u86cb\u767d\u5bb6\u65cf(glyceraldehyde-3-phosphate dehydrogenase protein family)\u7684\u6210\u54e1\u3002\u7518\u6cb9\u919b-3-\u78f7\u9178\u812b\u6c2b\u9176(Glyceraldehyde-3-phosphate dehydrogenase)\u5df2\u88ab\u78ba\u5b9a\u70ba\u4e00\u7a2emoonlighting protein\uff0c\u56e0\u70ba\u5b83\u80fd\u5920\u57f7\u884cmechanistically distinct functions\u3002 GAPDH \u7684\u7522\u7269\u50ac\u5316\u78b3\u6c34\u5316\u5408\u7269\u4ee3\u8b1d(carbohydrate metabolism)\u4e2d\u91cd\u8981\u7684\u80fd\u91cf\u7522\u751f\u6b65\u9a5f\uff0c\u5373\u5728\u7121\u6a5f\u78f7\u9178\u9e7d(inorganic phosphate)\u548c\u7159\u9170\u80fa\u817a\u560c\u5464\u4e8c\u6838\u82f7\u9178 (nicotinamide adenine dinucleotide , NAD) \u5b58\u5728\u4e0b 3-\u78f7\u9178\u7518\u6cb9\u919b(Glyceraldehyde-3-phosphate dehydrogenase)\u7684\u53ef\u9006\u6c27\u5316\u78f7\u9178\u5316\u3002\u5df2\u53e6\u5916\u9451\u5b9a\u7518\u6cb9\u919b-3-\u78f7\u9178\u812b\u6c2b\u9176(Glyceraldehyde-3-phosphate dehydrogenase)\u5728\u7d30\u80de\u6838\u4e2d\u5177\u6709\u5c3f\u5627\u5576DNA\u7cd6\u57fa\u5316\u9176(uracil DNA glycosylase activity)\u6d3b\u6027\u3002\u6b64\u5916\uff0c\u7518\u6cb9\u919b-3-\u78f7\u9178\u812b\u6c2b\u9176(Glyceraldehyde-3-phosphate dehydrogenase) \u542b\u6709\u5c0d\u5927\u8178\u687f\u83cc(E. coli)\u3001\u9285\u7da0\u5047\u55ae\u80de\u83cc(P. aeruginosa)\u548c\u767d\u8272\u5ff5\u73e0\u83cc(C. albicans)\u5177\u6709\u6297\u83cc\u6d3b\u6027(antimicrobial activity)\u7684\u80bd\u3002\u5c0d\u5c0f\u9f20\u4e2d\u985e\u4f3c\u86cb\u767d\u8cea\u7684\u7814\u7a76\u5df2\u7d93\u78ba\u5b9a\u4e86\u591a\u7a2e\u9644\u52a0\u529f\u80fd\uff0c\u5305\u62ec\u6838\u86cb\u767d\u7684\u4e9e\u785d\u57fa\u5316(nitrosylation)\u3001mRNA \u7a69\u5b9a\u6027(RNA stability)\u7684\u8abf\u7bc0\u4ee5\u53ca\u4f5c\u70ba\u5de8\u566c\u7d30\u80de(macrophage)\u7d30\u80de\u8868\u9762\u7684\u8f49\u9435\u86cb\u767d\u53d7\u9ad4(transferrin receptor)\u3002\u8a31\u591a\u8207\u8a72\u57fa\u56e0\u5ea7\u76f8\u4f3c\u7684\u5047\u57fa\u56e0(pseudogenes)\u5b58\u5728\u65bc\u4eba\u985e\u57fa\u56e0\u7d44(human genome)\u4e2d\u3002\u9078\u64c7\u6027\u526a\u63a5(splicing)\u5c0e\u81f4\u591a\u500b\u8f49\u9304\u8b8a\u9ad4(ranscript variants)\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0GAPDH\u591a\u682a\u6297\u9ad4\uff0c\u514d\u75ab\u539fSynthesized peptide derived from the N-terminal region of human GAPDH\u3002\u901a\u904e\u4f7f\u7528\u7279\u7570\u6027\u514d\u75ab\u539f\u7684\u89aa\u548c\u5c64\u6790\u5f9e\u5154\u6297\u8840\u6e05\u4e2d\u89aa\u548c\u7d14\u5316\u6297\u9ad4\u3002\u8fa8\u8996\u7269\u7a2eHuman, Mouse, Rat\u3002\u53ef\u61c9\u7528\u65bcELISA, IHC-P, WB\u5be6\u9a57\u3002\u6700\u4f73\u5de5\u4f5c\u7a00\u91cb\u5ea6\u61c9\u7531\u7814\u7a76\u8005\u901a\u904e\u5be6\u9a57\u78ba\u5b9a\u3002\u5efa\u8b70\u7684\u8d77\u59cb\u7a00\u91cb\u5ea6\u5982\u4e0b\uff1aWB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000).\u3002\u6fc3\u5ea61mg\/ml\uff0c\u4fdd\u5b58\u65bcPBS containing 50% Glycerol, 0.5% BSA and 0.05% Sodium Azide.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nGAPDH Polyclonal Antibody<\/a><\/td>\n<\/tr>\n
Immunogen<\/strong><\/td>\nSynthesized peptide derived from the N-terminal region of human GAPDH<\/td>\n<\/tr>\n
Host<\/strong><\/td>\nRabbit<\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nHuman, Mouse, Rat<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA, IHC-P, WB<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nOptimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.<\/td>\n<\/tr>\n
Clonality<\/strong><\/td>\nPolyclonal<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nGAPDH; GAPD; CDABP0047; OK\/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLiquid solution<\/td>\n<\/tr>\n
Concentration<\/strong><\/td>\n1 mg\/ml<\/td>\n<\/tr>\n
Storage buffer<\/strong><\/td>\nPBS containing 50% Glycerol, 0.5% BSA and 0.05% Sodium Azide.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9810\u67d3protein marker<\/a> | \u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\u00a0<\/a> | \u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n

Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u5ea6\u7279\u7570\u6027\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\uff0ctag antibody\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\u5982 His tag\u3001GST tag\u3001S tag\u3001HA tag\u3001DDDDK tag\u3001Myc tag\u3001MBP tag\u548c mCherry tag\u7b49\u55ae\/\u591a\u682aTag\u6297\u9ad4\u3002\u53e6\u5177\u751f\u7269\u7d20(Biotin)\u3001HRP\u3001AbFluor\u2122 \u67d3\u6599\u3001\u74ca\u8102\u7cd6(agarose)\u548c\u78c1\u73e0\u5076\u806f\u7269(magnetic beads)\u6a19\u8a8c\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat.No<\/td>\nName<\/td>\nSize1<\/td>\n<\/tr>\n
KTD104-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Magnetic Beads)<\/td>\n20T<\/td>\n<\/tr>\n
KTD105-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Agarose)<\/td>\n20T<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25122<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
KTI2014<\/td>\nIPKine\u2122 Anti-DDDDK Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2024<\/td>\nIPKine\u2122 Anti-GFP Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2034<\/td>\nIPKine\u2122 Anti-GST Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2044<\/td>\nIPKine\u2122 Anti-HA Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2064<\/td>\nIPKine\u2122 Anti-Myc Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
ABT2013<\/td>\nAgarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2014<\/td>\nMagnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2024<\/td>\nMagnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2023<\/td>\nAgarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2043<\/td>\nAgarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2044<\/td>\nMagnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2053<\/td>\nAgarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2054<\/td>\nMagnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2063<\/td>\nAgarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2064<\/td>\nMagnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2173<\/td>\nAgarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2174<\/td>\nMagnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2011<\/td>\nAnti-DDDDK Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2015<\/td>\nHRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2025<\/td>\nHRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2021<\/td>\nAnti-GFP Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2030<\/td>\nAnti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2035<\/td>\nHRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2041<\/td>\nAnti-HA Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2045<\/td>\nHRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2050<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2051<\/td>\nAnti-His Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2055<\/td>\nHRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2060<\/td>\nAnti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2061<\/td>\nAnti-Myc Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2065<\/td>\nHRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2170<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2070<\/td>\nAnti-MBP Tag Mouse Monoclonal Antibody (9Y5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2080<\/td>\nAnti-mCherry Tag Mouse Monoclonal Antibody (9D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2090<\/td>\nAnti-E2 Tag Mouse Monoclonal Antibody (12T4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2100<\/td>\nAnti-HSV Tag Mouse Monoclonal Antibody (16T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2110<\/td>\nAnti-KT3 Tag Mouse Monoclonal Antibody (14D8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2120<\/td>\nAnti-RFP Tag Mouse Monoclonal Antibody (9D1)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2130<\/td>\nAnti-S Tag Mouse Monoclonal Antibody (9T10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2150<\/td>\nAnti-T7 Tag Mouse Monoclonal Antibody (6D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2160<\/td>\nAnti-Trx Tag Mouse Monoclonal Antibody (14D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2180<\/td>\nAnti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2190<\/td>\nAnti-CBP Tag Monoclonal Antibody (12H5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2200<\/td>\nAnti-TAP Tag Monoclonal Antibody (4H2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2210<\/td>\nAnti-Avi-Tag Monoclonal Antibody (5G11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2220<\/td>\nAnti-SRT-Tag Monoclonal Antibody (11G3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2230<\/td>\nAnti-Strep-Tag II Monoclonal Antibody (8C12)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2240<\/td>\nAnti-mStrawberry Monoclonal Antibody (4C9)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2250<\/td>\nAnti-EYFP-Tag Monoclonal Antibody (10T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2260<\/td>\nAnti-mOrange Monoclonal Antibody (9A10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2270<\/td>\nAnti-AmCyan Monoclonal Antibody (8T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2280<\/td>\nAnti-ECFP-Tag Monoclonal Antibody (6B11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2290<\/td>\nAnti-EBFP Monoclonal Antibody (8B5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2300<\/td>\nAnti-Nano-Tag9 Monoclonal Antibody (11T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

GAPDH Polyclonal Antibody | Abbkine\u8ca8\u865fABP52783 Rabbit an […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[476],"tags":[832],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32998"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32998"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32998\/revisions"}],"predecessor-version":[{"id":33004,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32998\/revisions\/33004"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32998"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32998"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32998"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}