{"id":32869,"date":"2022-06-14T14:22:59","date_gmt":"2022-06-14T06:22:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32869"},"modified":"2022-11-07T11:42:40","modified_gmt":"2022-11-07T03:42:40","slug":"%e4%ba%ba%e8%84%82%e7%a3%b7%e5%a3%81%e9%85%b8%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%b5%84-human-lipoteichoic-acid-lta-elisa-kit%e8%b2%a8%e8%99%9fabx257487","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32869","title":{"rendered":"\u4eba\u8102\u78f7\u58c1\u9178\u6aa2\u6e2c\u8a66\u5291\u7d44 | Human Lipoteichoic Acid (LTA) ELISA Kit\u8ca8\u865fabx257487"},"content":{"rendered":"

\u4eba\u8102\u78f7\u58c1\u9178\u6aa2\u6e2c\u8a66\u5291\u7d44 Human Lipoteichoic Acid (LTA) ELISA Kit\u8ca8\u865fabx257487<\/a><\/span><\/p>\n

\"Abbexa<\/p>\n

\u78f7\u58c1\u9178\uff08teichoic\u00a0acid\uff09\u662f\u9769\u862d\u6c0f\u967d\u6027\uff08G+\uff09\u83cc\u7d30\u80de\u58c1\u7279\u6b8a\u7d44\u4efd\uff0c\u7531\u6838\u7cd6\u9187\uff08Ribitol\uff09\u6216\u7518\u6cb9\uff08Glyocerol\uff09\u6b98\u57fa\u7d93\u7531\u78f7\u9178\u4e8c\u9375\u4e92\u76f8\u9023\u63a5\u800c\u6210\u7684\u591a\u805a\u7269\u3002\u662f\u4e00\u7a2e\u5f31\u9178\u6027\u7269\u8cea\u3002\u5176\u542b\u91cf\u6703\u96a8\u8457\u57f9\u990a\u57fa\u6210\u5206\u8b8a\u5316\u800c\u8b8a\u5316\uff0c\u4e00\u822c\u5360\u7d30\u80de\u58c1\u54c1\u8cea\u768410%\uff0c\u6709\u6642\u53ef\u63a5\u8fd150%\u3002\u6839\u64da\u5176\u5728\u7d30\u80de\u8868\u9762\u4e0a\u7684\u56fa\u5b9a\u65b9\u5f0f\uff0c\u78f7\u58c1\u9178\u53ef\u5206\u70ba\u58c1\u78f7\u58c1\u9178\uff08Wall teichoic acid\uff09\u548c\u819c\u78f7\u58c1\u9178\uff08Membrane teichoic acid\uff09\u5169\u7a2e\uff0c\u524d\u8005\u4e0d\u6df1\u5165\u8cea\u819c\uff0c\u5176\u672b\u7aef\u4ee5\u78f7\u9178\u4e8c\u916f\u9375\u8207\u80bd\u805a\u7cd6\u7684N-\u4e59\u91af\u80de\u58c1\u9178\u6b98\u57fa\u9023\u7d50\u3002\u819c\u78f7\u58c1\u9178\u53c8\u7a31\u8102\u78f7\u58c1\u9178\uff08Lipteichoic acid\uff09\u8de8\u904e\u80bd\u805a\u7cd6\u5c64\uff0c\u4ee5\u5176\u672b\u7aef\u78f7\u9178\u5171\u50f9\u9023\u63a5\u65bc\u8cea\u819c\u4e2d\u7cd6\u8102\uff08\u4f8b\u5982\u4e8c\u8461\u7cd6\u57fa\u4e8c\u91af\u57fa\u7518\u6cb9\uff09\u7684\u5be1\u7cd6\u57fa\u90e8\u5206\u3002\u8102\u78f7\u58c1\u9178\uff08Lipoteichoic Acid\uff0cLTA\uff09\u662f\u9769\u862d\u6c0f\u967d\u6027\u83cc\u7d30\u80de\u58c1\u7684\u4e3b\u8981\u6210\u5206\u3002\u9019\u4e9b\u6709\u6a5f\u9ad4\u6709\u4e00\u500b\u5167\uff08\u6216\u7d30\u80de\u8cea\uff09\u819c\uff0c\u5728\u5176\u5916\u90e8\u6709\u4e00\u500b\u539a\uff08\u9ad8\u905480 nm\uff09\u7684\u80bd\u805a\u7cd6\u5c64\u3002\u8102\u78f7\u58c1\u9178\u7684\u7d50\u69cb\u56e0\u9769\u862d\u6c0f\u967d\u6027\u83cc\u7684\u4e0d\u540c\u7a2e\u985e\u800c\u7570\uff0c\u53ef\u80fd\u542b\u6709\u9577\u93c8\u6838\u7cd6\u9187\u6216\u78f7\u9178\u7518\u6cb9\u3002\u8102\u78f7\u58c1\u9178\u901a\u904e\u4e8c\u91af\u57fa\u7518\u6cb9\u56fa\u5b9a\u5728\u7d30\u80de\u819c\u4e0a\u3002\u5b83\u5145\u7576\u81ea\u6eb6\u58c1\u9176\uff08\u80de\u58c1\u9178\u9176\uff09\u7684\u8abf\u7bc0\u5291\u3002\u4e14\u5177\u6709\u6297\u539f\u7279\u6027\uff0c\u80fd\u5920\u523a\u6fc0\u7279\u5b9a\u7684\u514d\u75ab\u53cd\u61c9\uff0c\u4e3b\u8981\u5728\u6eb6\u83cc\u9176\u3001\u767d\u7d30\u80de\u967d\u96e2\u5b50\u80bd\u6216\u03b2-\u5167\u91af\u80fa\u6297\u751f\u7d20\u8a98\u5c0e\u6eb6\u83cc\u5f8c\u5f9e\u7d30\u83cc\u7d30\u80de\u4e2d\u91cb\u653e\u51fa\u4f86\u3002<\/p>\n

\"\u78f7\u58c1\u9178\"<\/p>\n

Human Lipoteichoic Acid (LTA) ELISA Kit is an ELISA Kit for the in vitro quantitative measurement of Human Lipoteichoic Acid (LTA) concentrations in serum, plasma and other biological fluids.<\/p>\n

\u7522\u54c1\u4ecb\u7d39:<\/p>\n

\"abx257487_Human_Lipoteichoic_Acid_LTA_ELISA_Kit_graph-500x500\"<\/a><\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

Lipoteichoic acid (LTA) is a major constituent of the cell wall of gram-positive bacteria. These organisms have an inner (or cytoplasmic) membrane and, external to it, a thick (up to 80 nanometer) peptidoglycan layer. The structure of LTA varies between the different species of Gram positive bacteria and may contain long chains of ribitol or glycerol phosphate. LTA is anchored to the cell membrane via a diacylglycerol. It acts as regulator of autolytic wall enzymes (muramidases). It has antigenic properties being able to stimulate specific immune response and is released from the bacterial cells mainly after bacteriolysis induced by lysozyme, cationic peptides from leucocytes, or beta-lactam antibiotics.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Target<\/td>\nLipoteichoic Acid (LTA)<\/td>\n<\/tr>\n
Reactivity<\/td>\nHuman<\/td>\n<\/tr>\n
Tested Applications<\/td>\nELISA<\/td>\n<\/tr>\n
Recommended dilutions<\/td>\nOptimal dilutions\/concentrations should be determined by the end user.<\/td>\n<\/tr>\n
Storage<\/td>\nShipped at 4 \u00b0C. Upon receipt, store the kit according to the storage instruction in the kit’s manual.<\/td>\n<\/tr>\n
Validity<\/td>\nThe validity for this kit is 6 months.<\/td>\n<\/tr>\n
Stability<\/td>\nThe stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.<\/td>\n<\/tr>\n
UNSPSC Code<\/td>\n41116126<\/td>\n<\/tr>\n
Test Range<\/td>\n7.8 pg\/ml – 500 pg\/ml<\/td>\n<\/tr>\n
Sensitivity<\/td>\n< 4.69 pg\/ml<\/td>\n<\/tr>\n
Standard Form<\/td>\nLyophilized<\/td>\n<\/tr>\n
Detection Method<\/td>\nColorimetric<\/td>\n<\/tr>\n
Assay Type<\/td>\nSandwich<\/td>\n<\/tr>\n
Assay Data<\/td>\nQuantitative<\/td>\n<\/tr>\n
Sample Type<\/td>\nSerum, plasma and other biological fluids.<\/td>\n<\/tr>\n
Kit Components<\/td>\nThe kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.<\/p>\n
    \n
  • Pre-coated 96-Well Microplate<\/li>\n
  • Standard<\/li>\n
  • Standard Diluent Buffer<\/li>\n
  • Wash Buffer<\/li>\n
  • Detection Reagent A<\/li>\n
  • Detection Reagent B<\/li>\n
  • Diluent A<\/li>\n
  • Diluent B<\/li>\n
  • TMB Substrate<\/li>\n
  • Stop Solution<\/li>\n
  • Plate Sealer<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Material Required But Not Provided<\/td>\n\n
    \n
  • 37\u00b0C incubator<\/li>\n
  • Multi and single channel pipettes and sterile pipette tips<\/li>\n
  • Squirt bottle or automated microplate washer<\/li>\n
  • 1.5 ml tubes<\/li>\n
  • Distilled water<\/li>\n
  • Absorbent filter papers<\/li>\n
  • 100 ml and 1 liter graduated cylinders<\/li>\n
  • Microplate reader (wavelength: 450 nm)<\/li>\n
  • ELISA Shaker<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Sample Collection\/Preparation<\/td>\n\n
    \n
  • Serum: Samples should be collected into a serum separator tube. Coagulate the serum by leaving the tube undisturbed in a vertical position overnight at 4\u00b0C or at room temperature for up to 60 minutes. Centrifuge at approximately 1000 \u00d7 g for 20 min. Analyze the serum immediately or aliquot and store at -20\u00b0C or -80\u00b0C.<\/li>\n<\/ul>\n
      \n
    • Plasma: Collect plasma using heparin or EDTA as an anticoagulant. Centrifuge for 15 minutes at 1000 \u00d7 g within 30 minutes of collection. Assay immediately or aliquot and store at -20\u00b0C or -80\u00b0C. Avoid hemolysis and high cholesterol samples.<\/li>\n<\/ul>\n
        \n
      • Tissue homogenates: The preparation of tissue homogenates will vary depending upon tissue type \u2013 this is just an example. Rinse tissues with ice-cold PBS to remove the excess of blood. Weigh before homogenization. Finely mince tissues and homogenize with a tissue homogenizer on ice in PBS and sonicate the cell suspension. Centrifuge the homogenates at 5000 \u00d7 g for 5 min and collect the supernatant. Assay immediately or aliquot and store at -20\u00b0C.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Reagent Preparation<\/td>\nThis procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.<\/p>\n
    \n
  • 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.<\/li>\n<\/ul>\n
      \n
    • 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.<\/li>\n<\/ul>\n
        \n
      • 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Assay Procedure<\/td>\nThis procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.<\/p>\n
    \n
  • 1) Set standard, test samples and control wells.<\/li>\n<\/ul>\n
      \n
    • 2) Aliquot 100 \u00b5l of diluted standard into the standard wells.<\/li>\n<\/ul>\n
        \n
      • 3) Aliquot 100 \u00b5l of Standard Diluent buffer into control (zero) well.<\/li>\n<\/ul>\n
          \n
        • 4) Aliquot 100 \u00b5l of diluted samples into the sample wells. Incubate for 1 hr at 37 \u00b0C.<\/li>\n<\/ul>\n
            \n
          • 5) Aliquot 100 \u00b5l of Detection Reagent A to each well. Incubate for 1 hr at 37 \u00b0C.<\/li>\n<\/ul>\n
              \n
            • 6) Wash 3 times.<\/li>\n<\/ul>\n
                \n
              • 7) Aliquot 100 \u00b5l of Detection Reagent B to each well. Incubate for 90 mins at 37 \u00b0C.<\/li>\n<\/ul>\n
                  \n
                • 8) Wash 5 times.<\/li>\n<\/ul>\n
                    \n
                  • 9) Aliquot 90 \u00b5l of TMB Substrate to each well. Incubate for 10-20 mins at 37 \u00b0C.<\/li>\n<\/ul>\n
                      \n
                    • 10) Aliquot 50 \u00b5l of Stop Solution.<\/li>\n<\/ul>\n
                        \n
                      • 11) Measure the OD at 450 nm.<\/li>\n<\/ul>\n<\/td>\n<\/tr>\n
Availability<\/td>\nShipped within 5-12 working days.<\/td>\n<\/tr>\n
Note<\/td>\nThis product is for research use only.The range and sensitivity is subject to change. Please contact us for the latest product information. For accurate results, sample concentrations must be diluted to mid-range of the kit. If you require a specific range, please contact us in advance or write your request in your order comments.Please note that our ELISA and CLIA kits are optimised for detection of native samples, rather than recombinant proteins. We are unable to guarantee detection of recombinant proteins, as they may have different sequences or tertiary structures to the native protein.<\/td>\n<\/tr>\n
Plate coated with<\/td>\nAntibody<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u4eba\u8102\u78f7\u58c1\u9178\u6aa2\u6e2c\u8a66\u5291\u7d44 Human Lipoteichoic Acid (LTA) ELISA Kit\u8ca8\u865fabx […]<\/p>\n","protected":false},"author":3,"featured_media":29387,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[848],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32869"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32869"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32869\/revisions"}],"predecessor-version":[{"id":35814,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32869\/revisions\/35814"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29387"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32869"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32869"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32869"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}