{"id":32819,"date":"2022-06-09T14:18:47","date_gmt":"2022-06-09T06:18:47","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32819"},"modified":"2022-10-25T20:11:34","modified_gmt":"2022-10-25T12:11:34","slug":"%e7%b7%9a%e7%b2%92%e9%ab%94%e8%a4%87%e5%90%88%e7%89%a9%e2%85%a4%e6%b4%bb%e6%80%a7%e6%aa%a2%e6%b8%ac-abbkine-chekine-mitochondrial-complex-%e2%85%a4-activity-detection-kit%e8%b2%a8%e8%99%9fk","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32819","title":{"rendered":"\u7dda\u7c92\u9ad4\u8907\u5408\u7269\u2164\u6d3b\u6027\u6aa2\u6e2c | Abbkine CheKine\u2122 Mitochondrial complex \u2164 Activity Detection Kit\u8ca8\u865fKTB1890"},"content":{"rendered":"

\u7dda\u7c92\u9ad4\u8907\u5408\u7269\u2164\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2Abbkine CheKine\u2122 Mitochondrial complex \u2164 Activity Detection Kit | \u8ca8\u865fKTB1890<\/a><\/span><\/p>\n

\"\u539f\u7406\u8207\u77e5\u8b58\"<\/p>\n

\u7dda\u7c92\u9ad4\u547c\u5438\u93c8\u8907\u5408\u7269V(Mitochondrial respiratory chain complex V)\uff0c\u53c8\u7a31F1F0-ATP\u5408\u9176\uff0c\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269(microorganisms)\u548c\u57f9\u990a\u7d30\u80de\u7684\u7dda\u7c92\u9ad4\u4e2d\u3002\u8907\u5408\u7269 V \u7531\u5169\u500b\u4e9e\u57fa F1 \u548c F0 \u7d44\u6210\u3002\u5b83\u53ef\u4ee5\u5229\u7528\u547c\u5438\u93c8\u7522\u751f\u7684\u8cea\u5b50\u7684\u96fb\u5316\u5b78\u68af\u5ea6\u50ac\u5316ATP\u7684\u5408\u6210\uff0c\u4e5f\u53ef\u4ee5\u5728\u53ef\u9006\u904e\u7a0b\u4e2d\u6c34\u89e3ATP\u3002\u6b64\u5916\uff0c\u8907\u5408\u7269V\u4e5f\u5b58\u5728\u65bc\u8449\u7da0\u9ad4(chloroplasts)\u3001\u7570\u990a\u7d30\u83cc(heterotrophic bacteria)\u548c\u5149\u5408\u7d30\u83cc(photosynthetic bacteria)\u4e2d\u3002\u8907\u5408\u7269V(Complex V)\u662f\u7dda\u7c92\u9ad4\u6c27\u5316\u78f7\u9178\u5316(mitochondrial oxidative phosphorylation)\u548c\u8449\u7da0\u9ad4\u5149\u5408\u78f7\u9178\u5316(chloroplast photosynthetic phosphorylation)\u5408\u6210ATP\u7684\u95dc\u9375\u9176\u3002<\/p>\n

\"\u7522\u54c1\u7279\u9ede2\"<\/p>\n

Abbkine CheKine\u2122 \u7dda\u7c92\u9ad4\u8907\u5408\u7269\u2164\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08Abbkine CheKine\u2122 Mitochondrial complex \u2164 Activity Detection Kit, \u6bd4\u8272\u6cd5\uff09\u662f\u5c08\u9580\u70ba\u6aa2\u6e2c\u8907\u5408\u7269 I \u6d3b\u6027(complex I activity)\u800c\u958b\u767c\u7684\u3002\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u52a0\u9748\u654f\u6e96\u78ba\u3002\u8907\u5408\u7269\u2164\u6c34\u89e3ATP\u7522\u751fADP\u548cPi\u3002\u8907\u5408\u7269\u2164(complex \u2164)\u7684\u6d3b\u6027\u662f\u901a\u904e\u6e2c\u91cfPi\u589e\u52a0\u7387\u4f86\u78ba\u5b9a\u7684\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Mitochondrial complex \u2164 Activity Assay Kit (colorimetric)<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine CheKine\u2122 Mitochondrial complex \u2164 Activity Detection Kit (colorimetric ) is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. complex \u2164 hydrolyzes ATP to produce ADP and Pi. The activity of complex \u2164 is determined by measuring Pi increase rate.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Reagent\u2160
\n\u2022 Reagent \u2161
\n\u2022 Reagent \u2162
\n\u2022 Reagent \u2163
\n\u2022 Reagent \u2164
\n\u2022 Reagent \u2165
\n\u2022 Reagent \u2166
\n\u2022 Reagent \u2167
\n\u2022 Reagent \u2168
\n\u2022 Standard<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Detect the enzyme activity of complex V in plant tissues, animal tissues, cells and other samples.
\n\u2022 Provides detailed sample preparation and results calculation methods.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal.
\n\u2022 Avoid bubbles while mixing or redissolving components.
\n\u2022 Change pipette tips frequently to avoid cross contamination between components.
\n\u2022 Ensure that all components and equipment are at the proper temperature before starting the experiment.
\n\u2022 In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples.The samples should be diluted with Reagent\u2161and then measured again. If the absorbance values is too high (\uff1e1.5). Pay attention to multiply by the dilution factor when calculating the result.
\n\u2022 Reagent \u2162 has certain toxicity, and Reagent \u2168 is corrosive. Please take protective measures when operating..<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nThe kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u7522\u54c1\"<\/p>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n

\"\u76f8\u95dc\u7522\u54c1\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/u><\/strong><\/a> \u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/u><\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7dda\u7c92\u9ad4\u8907\u5408\u7269\u2164\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2Abbkine CheKine\u2122 Mitochondrial complex \u2164 […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[852],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32819"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32819"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32819\/revisions"}],"predecessor-version":[{"id":35333,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32819\/revisions\/35333"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32819"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32819"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32819"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}