{"id":32809,"date":"2022-06-08T16:01:57","date_gmt":"2022-06-08T08:01:57","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32809"},"modified":"2023-11-28T15:17:53","modified_gmt":"2023-11-28T07:17:53","slug":"%e5%a4%9a%e9%85%9a%e6%b0%a7%e5%8c%96%e9%85%b6%e6%aa%a2%e6%b8%ac%e5%a5%97%e7%b5%84-polyphenol-oxidase-ppo-activity-colorimetric-assay-kit-%e8%b2%a8%e8%99%9fktb1140","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32809","title":{"rendered":"\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44 | Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit \u8ca8\u865fKTB1140"},"content":{"rendered":"

\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44CheKine\u2122 Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit | \u8ca8\u865fKTB1140<\/a><\/span><\/strong><\/span><\/p>\n

\u591a\u915a\u6c27\u5316\u9176\uff08Polyphenol Oxidase , PPO\uff09\u5ee3\u6cdb\u5b58\u5728\u65bc\u52d5\u7269\u3001\u690d\u7269\u3001\u5fae\u751f\u7269(microorganisms)\u548c\u57f9\u990a\u7d30\u80de(cultured cells)\u4e2d\u3002\u4f5c\u70ba\u4e00\u7a2e\u542b\u9285\u6c27\u5316\u9176(copper-containing oxidase)\uff0cPPO\u53ef\u4ee5\u901a\u904e\u6c27\u5316\u4e00\u5143\u915a (oxidizing monohydric phenols)\u548c\u4e8c\u5143\u915a (dihydric phenols) \u7522\u751f\u918c (quinones)\u800c\u5f15\u8d77\u8910\u8b8a(browning)\u3002\u9019\u7a2e\u9176\u9084\u8207\u679c\u852c\u52a0\u5de5(fruit and vegetable processing)\u3001\u8336\u8449\u54c1\u8cea(tea quality)\u548c\u7d44\u7e54\u57f9\u990a(tissue culture)\u5bc6\u5207\u76f8\u95dc\u3002PPO\u591a\u915a\u6c27\u5316\u9176\u8a66\u5291\u76d2\u70ba\u6aa2\u6e2c\u8840\u6e05(serum)\u3001\u8840\u6f3f(plasma)\u3001\u7d44\u7e54(tissues)\u3001\u7d30\u80de(cells)\u3001\u690d\u7269(plants)\u548c\u7d30\u83cc(bacteria)\u7b49\u591a\u7a2e\u751f\u7269\u6a23\u54c1\u4e2d\u7684 PPO \u6d3b\u6027\u63d0\u4f9b\u4e86\u4e00\u7a2e\u7c21\u55ae\u7684\u65b9\u6cd5\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0c\u591a\u915a\u6c27\u5316\u9176\uff08PPO\uff09\u50ac\u5316\u5152\u8336\u915a\u751f\u6210\u918c\uff0c\u5176\u5728525 nm\u8655\u5177\u6709\u7279\u5fb5\u5438\u6536\u5cf0(absorption peak)\u3002\u5728 525 nm \u8655\u918c\u7684\u589e\u52a0\u7387\u53ef\u4ee5\u53cd\u6620 PPO \u7684\u6d3b\u6027\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Polyphenol Oxidase (PPO) Activity Colorimetric Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nThe kit provides a simple method for detecting PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria. In the assay, Polyphenol Oxidase (PPO) catalyzes catechol to generate quinone which has a characteristic absorption peak at 525 nm. The rate of quinone increase at 525 nm can reflect PPO activity.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nPPO; Polyphenol Oxidase<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Extraction Buffer
\n\u2022 Reagent I
\n\u2022 Reagent II
\n\u2022 Reagent III<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Determination of PPO activity in a variety of biological samples such as serum, plasma, tissues, cells, plants and bacteria.
\n\u2022 Detailed sample preparation and result calculation methods are provided.
\n\u2022 Simple operation steps.<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix or substitute reagents or materials from other kit lots or vendors.
\n\u2022 The sample preparation must be done on ice to prevent the enzyme inactivation, and the enzyme activity should be determined on the same day. avoid repeated freeze-thaw.
\n\u2022 Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
\n\u2022 The optimal reaction temperature of polyphenol oxidase for different samples is slightly different, and it can be adjusted between 25-37\u2103.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nStorage at 4\u00b0C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u591a\u915a\u6c27\u5316\u9176\u6aa2\u6e2c\u5957\u7d44CheKine\u2122 Polyphenol Oxidase (PPO) Activity Col […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[854],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32809"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32809"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32809\/revisions"}],"predecessor-version":[{"id":47420,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32809\/revisions\/47420"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32809"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32809"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}