{"id":32775,"date":"2022-06-01T17:27:22","date_gmt":"2022-06-01T09:27:22","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32775"},"modified":"2022-07-19T11:27:40","modified_gmt":"2022-07-19T03:27:40","slug":"v5%e5%96%ae%e6%a0%aa%e6%8a%97%e9%ab%94-abbkine%e8%b2%a8%e8%99%9fabt2170","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32775","title":{"rendered":"V5\u55ae\u682a\u6297\u9ad4 | Abbkine\u8ca8\u865fABT2170"},"content":{"rendered":"

V5\u55ae\u682a\u6297\u9ad4\u00a0|\u00a0<\/strong>Abbkine\u8ca8\u865fABT2170<\/strong><\/a><\/span><\/p>\n

Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/p>\n

V5 \u8868\u4f4d\u6a19\u7c64(\u00a0V5 epitope tag)\u4f86\u6e90\u65bc\u7334\u75c5\u6bd2 5 (SV5) \u526f\u7c98\u75c5\u6bd2 P \u548c V \u86cb\u767d\u4e0a\u7684\u4e00\u500b\u5c0f\u8868\u4f4d (Pk)\u3002 V5\u6a19\u7c64\u6297\u9ad4\u6709\u52a9\u65bc\u6aa2\u6e2c\u91cd\u7d44\u86cb\u767d(recombinant proteins)\uff0c\u5176\u4e2d\u4e00\u4e9b\u5305\u62ec\u8de8\u819c(transmembrane )\u548c\u5206\u6ccc\u86cb\u767d\u878d\u5408\u86cb\u767d(secreted proteins fusion protein)\u3002\u9019\u7a2e V5 \u6a19\u7c64\u6297\u9ad4\u662f\u901a\u904e\u514d\u75ab\u8207 KLH \u5076\u806f\u7684\u5408\u6210\u80bd GKPIPNPLLGLDST \u88fd\u5099\u7684\u3002The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag antibody can be helpful in detecting the recombinant proteins, some of which includea transmembrane and secreted proteins fusion protein. This V5-tag antibody is prepared by immunizing synthetic peptide GKPIPNPLLGLDST coupled to KLH.<\/p>\n

\"Fig.<\/p>\n

\"\u7522\u54c1\u4ecb\u7d39\"<\/p>\n

Abbkine\u63d0\u4f9b\u4e00\u7cfb\u5217\u9ad8\u5ea6\u7279\u7570\u6027\u55ae\u514b\u9686\u548c\u591a\u514b\u9686\u6297\u9ad4\uff0ctag antibody\u6297\u9ad4\u7cfb\u5217\u5546\u54c1\u5982 His tag\u3001GST tag\u3001S tag\u3001HA tag\u3001DDDDK tag\u3001Myc tag\u3001MBP tag\u548c mCherry tag\u7b49\u55ae\/\u591a\u682aTag\u6297\u9ad4\u3002\u53e6\u5177\u751f\u7269\u7d20(Biotin)\u3001HRP\u3001AbFluor\u2122 \u67d3\u6599\u3001\u74ca\u8102\u7cd6(agarose)\u548c\u78c1\u73e0\u5076\u806f\u7269(magnetic beads)\u6a19\u8a8c\u3002\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong>\u00a0DYKDDDDK \u80bd\uff08Flag-tag\uff09\u662f\u4e00\u7a2e\u591a\u80bd\u86cb\u767d\u8cea\u6a19\u7c64(polypeptide protein tag)\uff0c\u53ef\u4ee5\u4f7f\u7528\u91cd\u7d44 DNA \u6280\u8853(recombinant DNA technology)\u6dfb\u52a0\u5230\u86cb\u767d\u8cea\u4e2d\u3002\u5b83\u53ef\u7528\u65bc\u89aa\u548c\u5c64\u6790(affinity chromatography)\uff0c\u7136\u5f8c\u7528\u65bc\u5c07\u91cd\u7d44\u7684\u904e\u8868\u9054\u86cb\u767d\u8207\u5bbf\u4e3b\u751f\u7269\u8868\u9054\u7684\u91ce\u751f\u578b\u86cb\u767d(wild-type protein)\u5206\u96e2\u3002\u5b83\u9084\u53ef\u7528\u65bc\u5206\u96e2\u5177\u6709\u591a\u500b\u4e9e\u57fa(multiple subunits)\u7684\u86cb\u767d\u8cea\u8907\u5408\u7269(protein complexes)\u3002\u6297\u9ad4\u4e4b\u4fdd\u5b58<\/strong>:<\/strong>\u00a0\u8caf\u5b58\u65bc-20\u00b0C\u907f\u514d\u53cd\u8986\u51b7\u51cd\u89e3\u51cd\u3002<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

His Tag\u55ae\u682a\u6297\u9ad4(HRP)\u00a0<\/strong><\/a>\u00a0|\u00a0Flag-tag\u55ae\u682a\u6297\u9ad4(HRP<\/strong>)<\/strong><\/a>\u00a0|\u00a0GFP\u55ae\u682a\u6297\u9ad4<\/a>(HRP) |\u00a0GST\u55ae\u682a\u6297\u9ad4<\/a>(HRP) |\u00a0HA\u55ae\u682a\u6297\u9ad4<\/a>(HRP)\u9810\u67d3protein marker<\/a>\u00a0|\u00a0\u5feb\u901f\u578b\u86cb\u767d\u8cea\u67d3\u8272\u5291\u00a0<\/a>\u00a0|<\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/a><\/td>\n<\/tr>\n
Immunogen<\/td>\nSynthetic Peptide<\/td>\n<\/tr>\n
Host<\/td>\nMouse<\/td>\n<\/tr>\n
Applications<\/td>\nIF, IP, WB<\/td>\n<\/tr>\n
Applications notes<\/td>\nOptimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:5000), IF (1:1000), IP (1:200).<\/td>\n<\/tr>\n
Clonality<\/td>\nMonoclonal<\/td>\n<\/tr>\n
Preparation method<\/td>\nThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen<\/td>\n<\/tr>\n
Alternative<\/td>\nGKPIPNPLLGLDST epitope; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u76f8\u95dc\u8a66\u5291\"<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat.No<\/td>\nName<\/td>\nSize1<\/td>\n<\/tr>\n
KTD104-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Magnetic Beads)<\/td>\n20T<\/td>\n<\/tr>\n
KTD105-EN<\/td>\nUniversal IP\/Co-IP Toolkit (Agarose)<\/td>\n20T<\/td>\n<\/tr>\n
A25012<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25022<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG LCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25112<\/td>\nIPKine\u2122 HRP, Goat Anti-Mouse IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25122<\/td>\nIPKine\u2122 HRP, Mouse Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
A25222<\/td>\nIPKine\u2122 HRP, Goat Anti-Rabbit IgG HCS<\/td>\n100 \u03bcL<\/td>\n<\/tr>\n
KTI2014<\/td>\nIPKine\u2122 Anti-DDDDK Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2024<\/td>\nIPKine\u2122 Anti-GFP Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2034<\/td>\nIPKine\u2122 Anti-GST Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2044<\/td>\nIPKine\u2122 Anti-HA Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
KTI2064<\/td>\nIPKine\u2122 Anti-Myc Magnetic IP Kit<\/td>\n20T<\/td>\n<\/tr>\n
ABT2013<\/td>\nAgarose Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2014<\/td>\nMagnetic Beads Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2024<\/td>\nMagnetic Beads Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2023<\/td>\nAgarose Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2043<\/td>\nAgarose Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2044<\/td>\nMagnetic Beads Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2053<\/td>\nAgarose Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2054<\/td>\nMagnetic Beads Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2063<\/td>\nAgarose Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2064<\/td>\nMagnetic Beads Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2173<\/td>\nAgarose Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2174<\/td>\nMagnetic Beads Conjugated Anti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n1 mL<\/td>\n<\/tr>\n
ABT2010<\/td>\nAnti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2011<\/td>\nAnti-DDDDK Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2015<\/td>\nHRP Conjugated Anti-DDDDK Tag Mouse Monoclonal Antibody (1B10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2020<\/td>\nAnti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2025<\/td>\nHRP Conjugated Anti-GFP Tag Mouse Monoclonal Antibody (3D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2021<\/td>\nAnti-GFP Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2030<\/td>\nAnti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2035<\/td>\nHRP Conjugated Anti-GST Tag Mouse Monoclonal Antibody (2A8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2040<\/td>\nAnti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2041<\/td>\nAnti-HA Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2045<\/td>\nHRP Conjugated Anti-HA Tag Mouse Monoclonal Antibody (4F6)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2050<\/td>\nAnti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2051<\/td>\nAnti-His Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2055<\/td>\nHRP Conjugated Anti-His Tag Mouse Monoclonal Antibody (5C3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2060<\/td>\nAnti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2061<\/td>\nAnti-Myc Tag Rabbit Polyclonal Antibody<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2065<\/td>\nHRP Conjugated Anti-Myc Tag Mouse Monoclonal Antibody (2D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2170<\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2070<\/td>\nAnti-MBP Tag Mouse Monoclonal Antibody (9Y5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2080<\/td>\nAnti-mCherry Tag Mouse Monoclonal Antibody (9D3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2090<\/td>\nAnti-E2 Tag Mouse Monoclonal Antibody (12T4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2100<\/td>\nAnti-HSV Tag Mouse Monoclonal Antibody (16T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2110<\/td>\nAnti-KT3 Tag Mouse Monoclonal Antibody (14D8)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2120<\/td>\nAnti-RFP Tag Mouse Monoclonal Antibody (9D1)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2130<\/td>\nAnti-S Tag Mouse Monoclonal Antibody (9T10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2150<\/td>\nAnti-T7 Tag Mouse Monoclonal Antibody (6D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2160<\/td>\nAnti-Trx Tag Mouse Monoclonal Antibody (14D4)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2180<\/td>\nAnti-VSV-G-Tag Mouse Monoclonal Antibody (14D2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2190<\/td>\nAnti-CBP Tag Monoclonal Antibody (12H5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2200<\/td>\nAnti-TAP Tag Monoclonal Antibody (4H2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2210<\/td>\nAnti-Avi-Tag Monoclonal Antibody (5G11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2220<\/td>\nAnti-SRT-Tag Monoclonal Antibody (11G3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2230<\/td>\nAnti-Strep-Tag II Monoclonal Antibody (8C12)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2240<\/td>\nAnti-mStrawberry Monoclonal Antibody (4C9)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2250<\/td>\nAnti-EYFP-Tag Monoclonal Antibody (10T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2260<\/td>\nAnti-mOrange Monoclonal Antibody (9A10)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2270<\/td>\nAnti-AmCyan Monoclonal Antibody (8T2)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2280<\/td>\nAnti-ECFP-Tag Monoclonal Antibody (6B11)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2290<\/td>\nAnti-EBFP Monoclonal Antibody (8B5)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n
ABT2300<\/td>\nAnti-Nano-Tag9 Monoclonal Antibody (11T3)<\/td>\n50 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

V5\u55ae\u682a\u6297\u9ad4\u00a0|\u00a0Abbkine\u8ca8\u865fABT2170 Anti-V5 Tag Mouse Monoclonal […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[856],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32775"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32775"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32775\/revisions"}],"predecessor-version":[{"id":32780,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32775\/revisions\/32780"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32775"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32775"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32775"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}