{"id":32669,"date":"2022-05-20T11:18:51","date_gmt":"2022-05-20T03:18:51","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32669"},"modified":"2024-08-01T14:53:13","modified_gmt":"2024-08-01T06:53:13","slug":"%e8%9b%8b%e7%99%bd%e8%b3%aa%e9%9b%bb%e6%b3%b3%e6%a8%99%e6%ba%96%e6%b6%b2-%e8%9b%8b%e7%99%bd%e8%b3%aa%e5%88%86%e5%ad%90%e9%87%8f%e6%a8%99%e6%ba%96%e6%b6%b2-blu12-blueye-prestained-protein-ladder","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32669","title":{"rendered":"\u86cb\u767d\u8cea\u96fb\u6cf3\u6a19\u6e96\u6db2 | \u86cb\u767d\u8cea\u5206\u5b50\u91cf\u6a19\u6e96\u6db2 Blu12 (BLUeye) Prestained Protein Ladder\uff0811 to 245 kDa\uff09\u8ca8\u865fPM007-0500\/PMB12-0500"},"content":{"rendered":"

\u86cb\u767d\u8cea\u96fb\u6cf3\u6a19\u6e96\u6db2 | \u86cb\u767d\u8cea\u5206\u5b50\u91cf\u6a19\u6e96\u6db2 Blu12 (BLUeye) Prestained Protein Ladder\uff0811 to 245 kDa\uff09\u8ca8\u865fPM007-0500<\/a>\u00a0 (Genedirex)\u00a0<\/span><\/strong><\/p>\n

PMB12-0500 \/ PM007-0500<\/span><\/strong><\/span><\/h4>\n

\u4e00\u3001\u7522\u54c1\u4ecb\u7d39<\/strong><\/span><\/p>\n

\u9810\u67d3\u578b\u86cb\u767d\u8cea\u5206\u5b50\u91cf\u6a19\u6e96\u6db2<\/u>(Prestained Protein Ladder) Blu12 (BLUeye) Prestained Protein Ladder \u5728 SDS-\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u81a0\u96fb\u6cf3\u671f\u9593\u8ddf\u8e2a\u86cb\u767d\u8cea\u7684\u5927\u5c0f\u548c\u5206\u96e2\uff0c\u4f30\u8a08\u86cb\u767d\u8cea\u5927\u5c0f\u4e26\u9a57\u8b49 PVDF\u3001\u5c3c\u9f8d\u6216\u785d\u9178\u7e96\u7dad\u7d20\u819c\u4e0a\u7684\u86cb\u767d\u8cea\u8f49\u79fb\u6548\u7387\u3002(\u5206\u5b50\u91cf11kDa\u300117kDa\u300120kDa\u3001<\/span>25kDa<\/span>\u300135kDa\u300148kDa\u300163kDa\u3001<\/span><\/span>75kDa<\/span>\u3001100kDa\u3001135kDa\u3001180kDa\u3001245kDa<\/span>)
\n\"\"<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

\u4e8c\u3001\u7522\u54c1\u8aaa\u660e<\/strong><\/span><\/p>\n

Blu12 (BLUeye) Prestained Protein Ladder\u662f\u4e00\u7a2e\u5373\u7528\u578b\u9810\u67d3\u5206\u5b50\u91cf\u6a19\u6e96\u6db2\uff0c\u64c1\u6709\u4e09\u7a2e\u4e0d\u540c\u984f\u8272\u7684\u9810\u67d3\u578b\u86cb\u767d\u6a19\u8a18 (\u5176\u4e2d 1 \u689d 25 kDa \u7684\u7da0\u8272\u689d\u5e36\u548c 75 kDa \u7684\u7d05\u8272\u689d\u5e36\u4f5c\u70ba\u53c3\u8003\u689d\u5e36) \uff0c\u670912\u7a2e\u4e0d\u540c\u5206\u5b50\u91cf\u86cb\u767d\u6a19\u793a\uff0c\u5206\u5b50\u91cf\u7bc4\u570d\u5f9e11 \u5230 245 kDa\uff0c\u4f7f\u7528\u65b9\u4fbf\uff0c\u7121\u9808\u4efb\u4f55\u524d\u8655\u7406\u5373\u53ef\u4f7f\u7528\u3002
\n\"\"<\/p>\n

\u4e09\u3001\u7522\u54c1\u512a\u9ede<\/strong><\/span><\/p>\n

    \n
  1. \u7d05\u7da0\u85cd\u4e09\u7a2e\u984f\u8272(25 kDa \u7684\u7da0\u8272\u689d\u5e36\u548c 75 kDa \u7684\u7d05\u8272\u689d\u5e36\u4f5c\u70ba\u53c3\u8003\u689d\u5e36)
    \n2. \u5206\u5b50\u91cf\u7bc4\u570d\u5f9e11 \u5230 245 kDa
    \n3. -20\u2103\u4fdd\u5b58\u53ef\u9577\u9054\u5169\u5e74<\/li>\n<\/ol>\n

    The Blu12 (BLUeye) Prestained Protein Ladder is a combination of 12 pre-stained proteins with molecular weights from 11 to 245 kDa. The 12 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa serve as reference bands. The Blu12 (BLUeye) Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.<\/p>\n

    3 \u03bcl or 5 \u03bcl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 \u03bcl per well for general Western transferring.<\/p>\n

    Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.<\/strong><\/p>\n

     <\/p>\n

    Storage Bu\ufb00er\u00a0:\u00a0<\/strong>
    \nApproximately 0.1~0.4 mg\/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25\u00b0C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v\/v) Glycerol.<\/p>\n

     <\/p>\n

    Storage\u00a0:\u00a0<\/strong>
    \nStable for up to 2 weeks at 25\u00b0C.
    \nStable for up to 3 months at 4\u00b0C.
    \nStable for up to 24\u00a0months at -20\u00b0C.<\/p>\n

     <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
    CAT#<\/td>\nNAME<\/td>\nSize<\/td>\n<\/tr>\n
    BP001CU<\/td>\nBluPAD, LED Transilluminator, with mini Darkroom
    \n(CE\/ETL, Patent No: M543442)<\/td>\n    		Set<\/td>\n<\/tr>\n
    PMI09-0500<\/td>\nIRIS9 Plus Prestained Protein Ladder
    \n(9 pre-stained bands, 15-170 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMI11-0500<\/td>\nIRIS11 Prestained Protein Ladder
    \n(11 pre-stained bands, 3-260 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMB01-0500<\/td>\nBlu10 Plus Prestained Protein Ladder
    \n(10 pre-stained bands, 6.5-270 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMB10-0500<\/td>\nBlu10 Prestained Protein Ladder
    \n(10 pre-stained bands, 11-180 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMB11-0500<\/td>\nBlu11 Prestained Protein Ladder
    \n(11 pre-stained bands, 10-180 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMB12-0500<\/td>\nBlu12 Prestained Protein Ladder
    \n(12 pre-stained bands, 11-245 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMB13-0500<\/td>\nBlu13 Prestained Protein Ladder
    \n(13 pre-stained bands, 5-245 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    PMU12-0500<\/td>\nUNveil Unstained Protein Ladder, Broad Range
    \n(12 unstained bands, 10-200 kDa)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    CCH321-B100ML<\/td>\nUltraScence Pico Plus Western Substrate
    \n(Patent No: US10,711,185)<\/td>\n    		100 ml
    \n(50ml+50ml)<\/td>\n<\/tr>\n
    CCH345-B100ML<\/td>\nUltraScence Pico Ultra Western Substrate
    \n(Patent No: US10,711,185)<\/td>\n    		100 ml
    \n(50ml+50ml)<\/td>\n<\/tr>\n
    CCH365-B100ML<\/td>\nUltraScence Femto Western Substrate
    \n(Patent No: US10,711,185)<\/td>\n    		100 ml
    \n(50ml+50ml)<\/td>\n<\/tr>\n
    CCH375-B100ML<\/td>\nUltraScence Femto Plus Western Substrate
    \n(Patent No: US10,711,185)<\/td>\n    		100 ml
    \n(50ml+50ml)<\/td>\n<\/tr>\n
    PS002-B500ML<\/td>\nCOOMASSIEnano Protein Staining Solution, 500ml<\/td>\n500 ml<\/td>\n<\/tr>\n
    PS003-B500ML<\/td>\nPonceau S Protein Staining Solution<\/td>\n500 ml<\/td>\n<\/tr>\n
    BS001-B500ML<\/td>\nOneStep Blocker
    \nWestern Blocking Solution and Signal Enhancer<\/td>\n    		500 ml<\/td>\n<\/tr>\n
    TM001-B200ML<\/td>\nTMB ELISA Substrate<\/td>\n100 ml
    \n(50ml+50ml)<\/td>\n<\/tr>\n
    DM001-R500<\/td>\nBH 100bp DNA Ladder RTU
    \n(11 bands, 100-1,500bps)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    DM003-R500<\/td>\nBH 100bp DNA Ladder H3 RTU
    \n(12 bands, 100-3,000bps)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    DM012-R500<\/td>\nBH 50bp DNA Ladder RTU
    \n(17 bands, 50-1,500bps)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    DM015-R500<\/td>\nBH 1Kb Plus DNA Ladder RTU
    \n(13 bands, 100-10,000bps)<\/td>\n    		500 \u03bcl<\/td>\n<\/tr>\n
    DMF12-0100<\/td>\nOmniMARK 100 DNA Ladder RTU
    \n(12 bands, 100-3,000bps, fluorescent dye)<\/td>\n    		600 \u03bcl<\/td>\n<\/tr>\n
    DMF13-0100<\/td>\nOmniMARK 1K DNA Ladder RTU
    \n(13 bands, 100-10,000bps, fluorescent dye)<\/td>\n    		600 \u03bcl<\/td>\n<\/tr>\n
    LD011-1000<\/td>\nPrime Juice Preloading Fluorescent Stain<\/td>\n1 ml<\/td>\n<\/tr>\n
    LD003-0500<\/td>\nNovel Green Plus (20,000X) DNA Staining Reagent<\/td>\n500 \u03bcl<\/td>\n<\/tr>\n
    AGT002-0500<\/td>\nAgarose Tablets, 110 pcs<\/td>\n0.5g x 110<\/td>\n<\/tr>\n
    MB755-0100<\/td>\nAgarose Powder 100g -Molecular Biology Grade<\/td>\n100 g<\/td>\n<\/tr>\n
    MB755-0500<\/td>\nAgarose Powder 500g -Molecular Biology Grade<\/td>\n500 g<\/td>\n<\/tr>\n
    MB101-0500<\/td>\nTaq DNA Polymerase<\/td>\n500U\/100\u03bcl<\/td>\n<\/tr>\n
    DP001-0100<\/td>\nnanoTaq\u2122 Hot-Start DNA Polymerase<\/td>\n100 rxns
    \n500U\/100\u03bcl<\/td>\n<\/tr>\n
    DN001-0250<\/td>\n100 mM dNTP Set<\/td>\n4 x 250\u03bcl<\/td>\n<\/tr>\n
    DN0010<\/td>\n10 mM dNTP Mix<\/td>\n1 ml<\/td>\n<\/tr>\n
    MB200-P100<\/td>\n2X PCR SuperMix<\/td>\n100 rxns
    \n2 x 1.25mL<\/td>\n<\/tr>\n
    MBA01-0100<\/td>\nOmniPCR Supermix w Fluorescent dye<\/td>\n100 rxns
    \n(2.5ml)<\/td>\n<\/tr>\n
    RK001-0050<\/td>\nRScript\u2122 cDNA Synthesis Kit<\/td>\n50 rxns<\/td>\n<\/tr>\n
    RT001-0050<\/td>\nRScript\u2122 Reverse Transcriptase 10,000U<\/td>\n50 rxns
    \n10,000U\/50\u03bcl<\/td>\n<\/tr>\n
    RT001-0250<\/td>\nRScript\u2122 Reverse Transcriptase 50,000U<\/td>\n250 rxns
    \n50,000U\/250\u03bcl<\/td>\n<\/tr>\n
    RI001-0125<\/td>\nRIBOAssure\u2122 RNase Inhibitor 5,000U<\/td>\n125 rxns
    \n5,000U\/125\u03bcl<\/td>\n<\/tr>\n
    QPR01-0100<\/td>\nPanProbes\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
    \n(20 \u03bcl vol)<\/td>\n<\/tr>\n
    QPD01-0100<\/td>\nPanProbes\u2122 Universal qPCR MasterMix<\/td>\n100 reactions
    \n(20 \u03bcl vol)<\/td>\n<\/tr>\n
    QSR01-0100<\/td>\nPanGreen\u2122 One-Step RT-qPCR Kit<\/td>\n100 reactions
    \n(20 \u03bcl vol)<\/td>\n<\/tr>\n
    QSD01-0100<\/td>\nPanGreen\u2122 Universal SYBR\u00ae Green Mastermix<\/td>\n100 reactions
    \n(20 \u03bcl vol)<\/td>\n<\/tr>\n
    PDP01-0100<\/td>\nPlasmid miniPREP Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDP02-0020<\/td>\nPlasmid midiPREP Kit<\/td>\n20 rxns<\/td>\n<\/tr>\n
    PDP03-0010<\/td>\nPlasmid maxiPREP Kit<\/td>\n10 rxns<\/td>\n<\/tr>\n
    PDC01-0100<\/td>\nPCR Clean-Up & Gel Extraction Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC02-0100<\/td>\nGenomic DNA isolation dual kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC05-0100<\/td>\nDual Genomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC06-0100<\/td>\nDual Genomic DNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC09-0100<\/td>\nGenomic DNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC10-0100<\/td>\nGenomic DNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC11-0100<\/td>\nGenomic DNA Isolation Kit (Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC12-0100<\/td>\nGenomic DNA Isolation Kit (Paraffin-embedded tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM01-0100<\/td>\nMbead Buffy Coat Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM02-0100<\/td>\nMbead Tissue Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM03-0100<\/td>\nMbead Bacteria Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM05-0100<\/td>\nMbead Plant Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM06-0100<\/td>\nMbead Blood\/Cell Genomic DNA Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDR01-0100<\/td>\nGenomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDR02-0100<\/td>\nPlant Genomic DNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDR05-0100<\/td>\nGenomic DNA Isolation Reagent Kit(Blood\/Cultured Cell\/Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC04-0100<\/td>\nTotal RNA Isolation Kit (Blood\/Cultured Cell\/Fungus)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC07-0100<\/td>\nTotal RNA Isolation Kit (Plant)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC08-0100<\/td>\nTotal RNA Isolation Kit(Tissue)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDR03-0100<\/td>\nTotal RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDR04-0100<\/td>\nPlant Total RNA Isolation Reagent<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDC03-0100<\/td>\nVirus Nucleic Acid Isolation Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    PDM04-0100<\/td>\nMbead Virus Genomic Nucleic Acid Kit<\/td>\n100 rxns<\/td>\n<\/tr>\n
    QP019M-0050<\/td>\nCOVID-19 Mutation RT-qPCR Detection Kit (Alpha, Delta, Kappa)<\/td>\n50 rxns<\/td>\n<\/tr>\n
    QP019T-0100<\/td>\nCOVID-19 RT-qPCR Detection Kit Plus (TFDA)<\/td>\n100 rxns<\/td>\n<\/tr>\n
    QP019-0100<\/td>\nSARS-CoV-2\/COVID-19 RT-qPCR Detection Kit (RUO)<\/td>\n100 rxns<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    \u86cb\u767d\u8cea\u96fb\u6cf3\u6a19\u6e96\u6db2 | \u86cb\u767d\u8cea\u5206\u5b50\u91cf\u6a19\u6e96\u6db2 Blu12 (BLUeye) Prestained Protein […]<\/p>\n","protected":false},"author":3,"featured_media":32670,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5371,1,3],"tags":[860],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32669"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32669"}],"version-history":[{"count":4,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32669\/revisions"}],"predecessor-version":[{"id":49936,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32669\/revisions\/49936"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/32670"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32669"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32669"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}