{"id":32636,"date":"2022-05-18T13:52:48","date_gmt":"2022-05-18T05:52:48","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32636"},"modified":"2023-02-17T16:24:30","modified_gmt":"2023-02-17T08:24:30","slug":"%e5%87%8b%e4%ba%a1%e7%b4%b0%e8%83%9e%e5%88%86%e6%9e%90annexin-v%e6%b3%95%e5%92%8ctunel%e6%b3%95-apoptosis-assay-cocktail%e8%b2%a8%e8%99%9f-ktd102-en","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32636","title":{"rendered":"\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5) | Apoptosis Assay Cocktail\u8ca8\u865f KTD102-EN"},"content":{"rendered":"

\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5) | Apoptosis Assay Cocktail\u8ca8\u865f KTD102-EN<\/a><\/span><\/strong><\/span><\/p>\n

\u51cb\u4ea1\u7d30\u80de\u6703\u7522\u751f\u8a31\u591a\u91cd\u8981\u7684\u8b8a\u5316\uff0c\u5305\u62ec\u7d30\u80de\u6536\u7e2e(cell shrinkage)\u3001\u7d30\u80de\u8cea\u51dd\u96c6(cytoplasmic agglutination)\u3001DNA\u65b7\u88c2(DNA disruption)\u548c\u819c\u56ca\u6ce1(membrane vesicles)\u7684\u5f62\u6210\uff0c\u4e26\u5c0e\u81f4\u7d30\u80de\u6210\u5206\u5206\u96e2\u5f62\u6210\u51cb\u4ea1\u5c0f\u9ad4(apoptotic dies)\uff0c\u6700\u7d42\u88ab\u5de8\u566c\u7d30\u80de(macrophages)\u6216\u5be6\u8cea\u7d30\u80de\u541e\u566c(parenchyma cells)Abbkine \u57fa\u65bc\u65b0\u4e00\u4ee3 AbFluor\u2122 \u87a2\u5149\u67d3\u8272(AbFluor\u2122 fluorescent staining)\u548c\u9ad8\u6548\u91cd\u7d44\u86cb\u767d\u6280\u8853(recombinant protein technology)\uff0c\u958b\u767c\u4e86\u4e00\u7a2e\u96de\u5c3e\u9152\u5957\u88dd\uff0c\u53ef\u540c\u6642\u6aa2\u6e2c\u65e9\u671f\u548c\u665a\u671f\u7d30\u80de\u51cb\u4ea1\u7684\u4e0d\u540c\u968e\u6bb5\u3002 Vermes \u662f\u7b2c\u4e00\u500b\u4f7f\u7528 Annexin V \u67d3\u8272(Annexin V staining)\u7279\u7570\u6027\u9451\u5b9a\u5916\u7ffb\u7d30\u80de\u819c\u4e0a\u7684\u78f7\u8102\u9170\u7d72\u6c28\u9178 (PS) \u7684\u7814\u7a76\u4eba\u54e1\uff0cAnnexin V \u6aa2\u6e2c\u88ab\u5168\u7403\u7814\u7a76\u4eba\u54e1\u8a8d\u70ba\u662f\u6700\u7d93\u5178\u7684\u65b9\u6cd5\u3002\u6b64\u5916\uff0cDNA\u7247\u6bb5\u5316(DNA fragmentation)\u662f\u7d30\u80de\u51cb\u4ea1\u665a\u671f\u7684\u4e00\u500b\u91cd\u8981\u7279\u5fb5\uff0c\u57fa\u65bcDNA\u7247\u6bb5\u5316\u6aa2\u6e2c(DNA fragmentation detection)\u7684TUNEL\u65b9\u6cd5\u88ab\u8a8d\u70ba\u662f\u7d30\u80de\u51cb\u4ea1\u7814\u7a76\u7684\u53c8\u4e00\u91d1\u6a19\u6e96\u3002\u9069\u7528\u65bc\u7d44\u7e54(tissues)\u548c\u7d30\u80de(cells)\u7b49\u4e0d\u540c\u6a23\u54c1\u3002\u9ad8\u9748\u654f\u5ea6(High sensitivity)\u3001\u901a\u7528\u7684\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2(universal apoptosis detection kit)\uff0c\u53ef\u6709\u6548\u5340\u5206\u7d30\u80de\u51cb\u4ea1(apoptosis)\u8207\u58de\u6b7b(necrosis)\u3002\u540c\u6642\uff0c\u91dd\u5c0d\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u548c\u7d50\u679c\u7684\u975e\u6a19\u6e96\u5316\uff0c\u6211\u5011\u958b\u767c\u4e86\u4e00\u7a2e\u5c08\u5229\u7684\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u967d\u6027\u5c0d\u7167\u7269\u8cea(positive control)\uff0c\u4e26\u7d50\u5408\u5230\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u96de\u5c3e\u9152\u4e2d\u3002\u672c\u7522\u54c1\u53ef\u4f5c\u70ba\u7d30\u80de\u51cb\u4ea1\u7814\u7a76\u7684\u6a19\u6e96\u5316\u7522\u54c1\uff0c\u80fd\u6eff\u8db3\u5927\u90e8\u5206\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c(apoptosis detection)\u7684\u9700\u8981\u3002\u662f\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c(apoptosis detection)\u7684\u6700\u4f73\u9078\u64c7\u3002 Abbkine\u958b\u767c\u4e86\u4e00\u7a2e\u9ad8\u9748\u654f\u5ea6\u3001\u901a\u7528\u7684\u7d30\u80de\u51cb\u4ea1\u8a66\u5291\u76d2(universal apoptosis kit)\uff0c\u53ef\u540c\u6642\u6aa2\u6e2c\u65e9\u671f\u548c\u665a\u671f\u7d30\u80de\u51cb\u4ea1(apoptosis)\uff0c\u9069\u7528\u65bc\u7d44\u7e54\u548c\u7d30\u80de\uff0c\u6709\u6548\u5340\u5206\u7d30\u80de\u51cb\u4ea1\u548c\u58de\u6b7b\u3002\u672c\u7522\u54c1\u53ef\u4f5c\u70ba\u7d30\u80de\u51cb\u4ea1(apoptosis)\u7814\u7a76\u7684\u6a19\u6e96\u5316\u7522\u54c1\uff0c\u80fd\u6eff\u8db3\u5927\u90e8\u5206\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u7684\u9700\u8981\u3002\u662f\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c(apoptosis detection)\u7684\u6700\u4f73\u9078\u64c7\u3002<\/p>\n

\"\u66f4\u591a\u8cc7\u6599\"<\/p>\n

1 \u7528\u65bc\u7d30\u80de\u51cb\u4ea1\u7814\u7a76\u7684\u6a19\u6e96\u5316\u8a66\u5291\u76d2\u3002<\/p>\n

Annexin V\u6cd5\u548cTUNEL\u6cd5\u4f5c\u70ba\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u7684\u5169\u5927\u91d1\u6a19\u6e96\uff0c\u5e7e\u4e4e\u6db5\u84cb\u4e86\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u7684\u5927\u90e8\u5206\u8981\u6c42\u3002\u7368\u7279\u7684 Apoptosis Assay Cocktail \u4e0d\u50c5\u5305\u542b\u4e0a\u8ff0\u5169\u7a2e\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u800c\u4e14\u9084\u64c1\u6709\u5c08\u5229\u7684\u7d30\u80de\u51cb\u4ea1\u967d\u6027\u5c0d\u7167\uff0c\u5b8c\u7f8e\u89e3\u6c7a\u4e86\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u548c\u7d50\u679c\u4e0d\u898f\u7bc4\u7684\u554f\u984c\u3002<\/p>\n

2 \u9ad8\u9748\u654f\u5ea6\u3001\u901a\u7528\u7684\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2\u3002<\/p>\n

\u512a\u5316\u5f8c\uff0c\u8a72\u8a66\u5291\u76d2\u53ef\u540c\u6642\u6aa2\u6e2c\u65e9\u671f\u548c\u665a\u671f\u4e0d\u540c\u968e\u6bb5\u7684\u7d30\u80de\u51cb\u4ea1\uff0c\u9069\u7528\u65bc\u7d44\u7e54\u3001\u7d30\u80de\u7b49\u4e0d\u540c\u6a23\u672c\uff0c\u6709\u6548\u5340\u5206\u7d30\u80de\u51cb\u4ea1\u548c\u58de\u6b7b\u3002<\/p>\n

3 \u5ee3\u6cdb\u4f7f\u7528\u3002<\/p>\n

\u53ef\u7528\u65bc\u6d41\u5f0f\u7d30\u80de\u5100\u548c\u87a2\u5149\u986f\u5fae\u93e1\u89c0\u5bdf\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n
Component name<\/strong><\/td>\n\u89c4\u683c<\/strong><\/td>\n<\/tr>\n
Annexin V-AbFluor\u2122 488 Apoptosis Detection Kit<\/td>\n40 T<\/td>\n<\/tr>\n
TUNEL Apoptosis Detection Kit (Green Fluorescence)<\/td>\n20 T<\/td>\n<\/tr>\n
Apoptosis Positive Control<\/td>\n5 T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

HeLa cells were induced by inducers A and B as positive control group, and normal cells as negative control. Apoptosis detection cocktail kit was used for detection. Annexin V-Abfluor \u2122 488 has a high affinity for PS, and PS exposed through the outer side of the cell binds to the membrane of cells in the early apoptotic stage, which were marked fluorescent green and the nucleus were marked red fluorescent by PI after structural damage.<\/p>\n

\"\"<\/p>\n

Fig.1 Annexin V-AbFluor\u2122 488 Apoptosis Detection Kit was used to detect the apoptosis effect of HeLa cells after apoptosis induction<\/strong><\/p>\n

After Annexin V-Abfluor \u2122 488 labeling, the necrotic cells and apoptotic cells in early stage and late stage were accurately identified.<\/p>\n

\"\"<\/p>\n

Fig.2 Flow cytometry was used to detect the apoptosis rate by different concentrations of inducer A and B.<\/strong><\/p>\n

In the detection of apoptosis by TUNEL assay, deoxyribonucleotide terminal transferase (TdT) was used to label the deoxyribonucleotide – luciferase conjugates to the 3′- terminus of the DNA gap, and apoptosis was visualized.<\/p>\n

Fig.3 TUNEL Apoptosis Detection Kit (Green Fluorescence) was used to detect the apoptosis effect of HeLa cells after apoptosis induction.<\/strong><\/p>\n

\"\"<\/p>\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/strong><\/td>\nProduct name<\/strong><\/td>\nclassification<\/strong><\/td>\n<\/tr>\n
KTB1600<\/a><\/td>\nCheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1610<\/a><\/td>\nCheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1620<\/a><\/td>\nCheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1630<\/a><\/td>\nCheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1640<\/a><\/td>\nCheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1650<\/a><\/td>\nCheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1660<\/a><\/td>\nCheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/span><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u51cb\u4ea1\u7d30\u80de\u5206\u6790(Annexin V\u6cd5\u548cTUNEL\u6cd5) | Apoptosis Assay Cocktail\u8ca8\u865f […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[862],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32636"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32636"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32636\/revisions"}],"predecessor-version":[{"id":32642,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32636\/revisions\/32642"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32636"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32636"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32636"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}