{"id":32587,"date":"2022-05-10T16:41:28","date_gmt":"2022-05-10T08:41:28","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32587"},"modified":"2023-11-12T15:53:53","modified_gmt":"2023-11-12T07:53:53","slug":"chekine-%e7%be%a5%e5%9f%ba%e8%87%aa%e7%94%b1%e5%9f%ba%e6%b8%85%e9%99%a4%e8%83%bd%e5%8a%9b%e6%b8%ac%e5%ae%9a-hydroxyl-free-radical-scavenging-capacity-colorimetric-assay-kit-%e8%b2%a8","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32587","title":{"rendered":"CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6e2c\u5b9a | Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit \u8ca8\u865f KTB1091"},"content":{"rendered":"

CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u00a0<\/strong>CheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit \u8ca8\u865f<\/strong> KTB1091<\/a><\/span><\/p>\n

\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl radical)\u2022OH\uff0c\u662f\u6c2b\u6c27\u6839\u96e2\u5b50\uff08hydroxide ion , OH-)\uff0c\u9ad8\u53cd\u61c9\u6027\uff08\u5bb9\u6613\u8b8a\u6210\u7fa5\u57fa\uff09\u3002\u7fa5\u57fa\u81ea\u7531\u57fa(Hydroxyl free radicals)\u4f5c\u7528\u65bc\u9ad4\u5167\u7684\u86cb\u767d\u8cea(proteins)\u3001\u6838\u9178(nucleic acids)\u3001\u8102\u985e(lipids)\u7b49\u751f\u7269\u5206\u5b50\uff0c\u5c0d\u7d30\u80de\u9020\u6210\u640d\u5bb3\u7d50\u69cb(structure)\u548c\u529f\u80fd(function)\uff0c\u9032\u800c\u5c0e\u81f4\u9ad4\u5167\u4ee3\u8b1d\u7d0a\u4e82(metabolic disorders)\u4e26\u5f15\u8d77\u75be\u75c5\u3002\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b(Hydroxyl free radical scavenging capacity)\u662f\u6a23\u54c1\u6297\u6c27\u5316\u80fd\u529b\u7684\u91cd\u8981\u6307\u6a19\u4e4b\u4e00\uff0c\u5df2\u5ee3\u6cdb\u61c9\u7528\u65bc\u6297\u6c27\u5316\u4fdd\u5065\u54c1(antioxidant health products)\u548c\u85e5\u7269(drugs)\u7684\u7814\u7a76\u3002 CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2(CheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit, KTB1091)\u5c08\u70ba\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u7684\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b(Hydroxyl Free Radical Scavenging Capacity)\u800c\u958b\u767c\u3002\u64cd\u4f5c\u7c21\u55ae\u65b9\u4fbf\uff0c\u6aa2\u6e2c\u66f4\u52a0\u9748\u654f\u6e96\u78ba\u3002\u5728\u8a72\u6e2c\u5b9a\u4e2d\uff0cH2O2\/ Fe2+ \u7522\u751f\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl free radical)\u901a\u904e\u82ac\u9813\u53cd\u61c9(Fenton reaction)\u3002\u6c34\u694a\u9178(Salicylic acid)\u80fd\u6709\u6548\u6355\u6349\u751f\u6210\u7684\u7fa5\u57fa\u81ea\u7531\u57fa(hydroxyl free radical)\u4e26\u8207\u4e4b\u53cd\u61c9\u751f\u6210\u7522\u751f\u6700\u5927\u5438\u6536\u5cf0\u5728 520 nm \u7684 2,3-\u4e8c\u7fa5\u57fa\u82ef\u7532\u9178(2,3-dihydroxybenzoic acid)\u3002\u5728\u5177\u6709\u80fd\u529b\u7684\u7269\u8cea\u4e4b\u5f8c\u6e05\u9664\u7fa5\u57fa\u81ea\u7531\u57fa\uff0c\u5c0e\u81f4520nm\u5438\u5149\u5ea6\u4e0b\u964d\u3002 520 nm \u7684\u5438\u5149\u5ea6\u503c\u53ef\u4ee5\u53cd\u6620\u6a23\u54c1\u7684\u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u3002<\/p>\n

<\/p>\n

(\u6587\u737b\u5f15\u7528\u51fa\u81ea<\/a><\/span>) p@tFNA scavenged hydroxyl free radicals and intracellular ROS. (a) Total antioxidant capacity assay results of tFNA and p@tFNA. (b) Hydroxyl free radical scavenging capacity detection of tFNA and p@tFNA.<\/p>\n

<\/p>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Colorimetric Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nAbbkine CheKine\u2122 Hydroxyl Free Radical Scavenging Capacity Assay Kit (Colorimetric) (Salicylic Acid Method) is specially developed for the detection of Hydroxyl Free Radical Scavenging Capacity in various sample. The operation is simple and convenient, and the detection is more sensitive and accurate. In this assay, H2O2\/ Fe2+ generates hydroxyl free radical through the Fenton reaction. Salicylic acid can effectively capture the generated hydroxyl free radical and reacts with them to produce 2,3- dihydroxybenzoic acid with a maximum absorption peak at 520 nm. After the substances with the capacity to scavenge hydroxyl free radical, resulting in the decrease of 520 nm absorbance. The value of 520 nm absorbance can reflects the Hydroxyl Free Radical Scavenging Capacity of the sample.<\/td>\n<\/tr>\n
Kit components<\/strong><\/td>\n\u2022 Ferrous Salt
\n\u2022 H2O2
\n\u2022Salicylic Acid\u2022 Free-HSA Negative Control<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 simple, sensitive, rapid detection method.
\n\u2022 compatible to various biological samples such as plasma, serum,bacteria, animal \/plant tissues and cells and other biological fluids.
\n\u2022 Provides detailed sample preparation , results calculation methods and comprehensive results display.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n
CAT#<\/strong><\/td>\nProduct name<\/strong><\/td>\nclassification<\/strong><\/td>\n<\/tr>\n
KTB1600<\/a><\/td>\nCheKine\u2122 Reduced Glutathione (GSH) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1610<\/a><\/td>\nCheKine\u2122 Glutathione Oxidized (GSSG) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1620<\/a><\/td>\nCheKine\u2122 Glutathione Reductases (GR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1630<\/a><\/td>\nCheKine\u2122 Glutathione S-Transferase (GST) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1640<\/a><\/td>\nCheKine\u2122 Glutathione Peroxidase (GSH-Px) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1650<\/a><\/td>\nCheKine\u2122 Thioredoxin Reductase (TrxR) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n
KTB1660<\/a><\/td>\nCheKine\u2122 Thioredoxin Peroxidase (TPX) Colorimetric Assay Kit<\/td>\nCell metabolism<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a>| Abbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a>| \u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6e05\u9664\u80fd\u529b\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2 | \u00a0CheKine\u2122 Hydroxyl Free Rad […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262,3],"tags":[864],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32587"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32587"}],"version-history":[{"count":6,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32587\/revisions"}],"predecessor-version":[{"id":46811,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32587\/revisions\/46811"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32587"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32587"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}