{"id":32556,"date":"2022-05-05T13:28:34","date_gmt":"2022-05-05T05:28:34","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=32556"},"modified":"2023-12-01T11:48:47","modified_gmt":"2023-12-01T03:48:47","slug":"human-egf-protein%e9%87%8d%e7%b5%84%e8%9b%8b%e7%99%bd-abbkine%e8%b2%a8%e8%99%9fprp100159","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=32556","title":{"rendered":"Human EGF protein\u91cd\u7d44\u86cb\u767d | Abbkine\u8ca8\u865fPRP100159"},"content":{"rendered":"

\u7d30\u80de\u6fc0\u7d20(Cytokines)-Human EGF protein\u91cd\u7d44\u86cb\u767d<\/span><\/span><\/strong><\/span><\/p>\n

\u7d30\u80de\u6fc0\u7d20(Cytokines)\u662f\u4e00\u985e\u7368\u7279\u7684\u7d30\u80de\u8a0a\u865f\u50b3\u5c0e\u5206\u5b50\uff0c\u5728\u7d30\u80de\u589e\u6b96\u548c\u767c\u80b2\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u7d30\u80de\u56e0\u5b50Abbkine \u63d0\u4f9b\u7d30\u80de\u56e0\u5b50\uff0c\u5305\u62ec\u767d\u8840\u7403\u4ecb\u7d20( interleukin)\u3001\u8da8\u5316\u56e0\u5b50(chemokines)\u3001\u96c6\u843d\u523a\u6fc0\u56e0\u5b50(colony-stimulating factors, CSF)\u3001\u5e72\u64fe\u7d20(interferon)\u4ee5\u53ca\u8f49\u5316\u751f\u9577\u56e0\u5b50(transforming growth factor , TGF) \u548c\u816b\u7624\u58de\u6b7b\u56e0\u5b50(tumor necrosis factor , TNF) \u5bb6\u65cf\uff0c\u7528\u65bc\u764c\u75c7(cancer)\u3001\u7d30\u80de\u51cb\u4ea1(apoptosis)\u3001\u5167\u5206\u6ccc\u5b78(endocrinology)\u3001\u514d\u75ab\u5b78(immunology)\u3001\u795e\u7d93\u79d1\u5b78(neuroscience)\u3001\u86cb\u767d\u9176(proteases)\u3001\u548c\u5e79\u7d30\u80de\u7814\u7a76\u3002\u9019\u4e9b\u7d30\u80de\u6fc0\u7d20\u7d93\u904e\u5168\u7a0b\u54c1\u8cea\u7ba1\u5236\uff0c\u54c1\u8cea\u56b4\u683c\u9a57\u8b49\uff0c\u53ef\u4ee5\u6eff\u8db3\u79d1\u5b78\u5bb6\u5c0d\u9ad8\u54c1\u8cea\u3001\u7d14\u5ea6\u9ad8\u3001\u5167\u6bd2\u7d20\u542b\u91cf\u4f4e\u3001\u9ad8\u751f\u7269\u6d3b\u6027\u548c\u6709\u7af6\u722d\u529b\u7684\u50f9\u683c\u7684\u9700\u6c42\u3002<\/p>\n

Human EGF protein\u91cd\u7d44\u86cb\u767d |\u8ca8\u865fPRP100159<\/span><\/span><\/strong><\/a><\/span><\/p>\n

\"Fig.SDS-PAGE<\/p>\n

Publications(1)\u00a0<\/span><\/strong><\/span><\/a><\/p>\n

EGF \u662f EGF \u86cb\u767d\u5bb6\u65cf(EGF-family)\u7684\u5275\u59cb\u6210\u54e1\u3002\u8a72\u86cb\u767d\u8cea\u5bb6\u65cf\u7684\u6210\u54e1\u5177\u6709\u9ad8\u5ea6\u76f8\u4f3c\u7684\u7d50\u69cb(structural)\u548c\u529f\u80fd(functional)\u7279\u5fb5\u3002 EGF \u5305\u542b 9 \u500b EGF \u6a23\u7d50\u69cb\u57df(EGF-like domains)\u548c 9 \u500b LDL \u53d7\u9ad4 B \u985e\u91cd\u8907\u5e8f\u5217(LDL-receptor class B repeats)\u3002\u4eba EGF \u662f\u4e00\u7a2e 6045-Da \u7684\u86cb\u767d\u8cea\uff0c\u5177\u6709 53 \u500b\u6c28\u57fa\u9178\u6b98\u57fa(amino acid residues)\u548c\u4e09\u500b\u5206\u5b50\u5167\u4e8c\u786b\u9375(disulfide bonds)\u3002\u4f5c\u70ba\u4e00\u7a2e\u4f4e\u5206\u5b50\u591a\u80bd(low-molecular-weight polypeptide)\uff0cEGF\u6700\u521d\u662f\u5f9e\u5c0f\u9f20\u981c\u4e0b\u817a(submandibular gland)\u4e2d\u7d14\u5316\u800c\u4f86\uff0c\u6b64\u5f8c\u5728\u5305\u62ec\u981c\u4e0b\u817a(submandibular gland)\u3001\u816e\u817a(parotid gland)\u5728\u5167\u7684\u591a\u7a2e\u4eba\u9ad4\u7d44\u7e54\u4e2d\u5747\u6709\u767c\u73fe\u3002\u5b83\u4e5f\u5b58\u5728\u65bc\u4eba\u985e\u8840\u5c0f\u677f(platelets)\u3001\u5de8\u566c\u7d30\u80de(macrophages)\u3001\u5c3f\u6db2(urine)\u3001\u553e\u6db2(saliva)\u3001\u725b\u5976(milk)\u548c\u8840\u6f3f(plasma)\u4e2d\u3002 EGF \u662f\u4e00\u7a2e\u751f\u9577\u56e0\u5b50(growth factor)\uff0c\u53ef\u5728\u9ad4\u5167\u548c\u9ad4\u5916\u523a\u6fc0\u5404\u7a2e\u8868\u76ae(epidermal)\u548c\u4e0a\u76ae\u7d44\u7e54(epithelial tissues)\u7684\u751f\u9577\uff0c\u4ee5\u53ca\u7d30\u80de\u57f9\u990a\u7269\u4e2d\u67d0\u4e9b\u6210\u7e96\u7dad\u7d30\u80de\u7684\u751f\u9577\u3002\u5b83\u5c0e\u81f4\u7d30\u80de\u589e\u6b96(cellular proliferation)\u3001\u5206\u5316(differentiation)\u548c\u5b58\u6d3b (survival)\u3002\u553e\u6db2 EGF(Salivary EGF) \u4f3c\u4e4e\u4e5f\u53d7\u81b3\u98df\u7121\u6a5f\u7898(dietary inorganic iodine)\u7684\u8abf\u7bc0\uff0c\u5728\u7dad\u6301\u53e3\u98df\u7ba1\u548c\u80c3\u7d44\u7e54\u5b8c\u6574\u6027\u65b9\u9762\u4e5f\u8d77\u8457\u91cd\u8981\u7684\u751f\u7406\u4f5c\u7528\u3002 EGF\u901a\u904e\u8207\u7d30\u80de\u8868\u9762\u7684\u8868\u76ae\u751f\u9577\u56e0\u5b50(epidermal growth factor receptor)\u53d7\u9ad4\u4ee5\u9ad8\u89aa\u548c\u529b\u7d50\u5408\u4f75\u523a\u6fc0\u53d7\u9ad4\u7684\u5167\u5728\u86cb\u767d\u916a\u6c28\u9178\u6fc0\u9176(intrinsic protein-tyrosine kinase)\u6d3b\u6027\u800c\u8d77\u4f5c\u7528\u3002\u53cd\u904e\u4f86\uff0c\u916a\u6c28\u9178\u6fc0\u9176(tyrosine kinase)\u6d3b\u6027\u555f\u52d5\u4fe1\u865f\u8f49\u5c0e\u7d1a\u806f\uff0c\u5c0e\u81f4\u7d30\u80de\u5167\u767c\u751f\u5404\u7a2e\u751f\u5316\u8b8a\u5316\u2014\u2014\u7d30\u80de\u5167\u9223(calcium)\u6c34\u5e73\u5347\u9ad8\u3001\u7cd6\u9175\u89e3\u548c\u86cb\u767d\u8cea\u5408\u6210(protein synthesis)\u589e\u52a0\uff0c\u4ee5\u53ca\u67d0\u4e9b\u57fa\u56e0\u7684\u8868\u9054\u589e\u52a0\uff0c\u5305\u62ecEGFR\u2014\u2014\u6700\u7d42\u5c0e\u81f4 DNA \u5408\u6210(DNA synthesis)\u548c\u7d30\u80de\u589e\u6b96(cell proliferation)\u3002<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/p>\n

\u7522\u54c1\u4ecb\u7d39<\/strong>:<\/strong> Human EGF protein\u91cd\u7d44<\/strong>\u86cb\u767d<\/strong>\u3002 \u8868\u9054\u548c\u7d14\u5316\u6e90\u81ea\u6210\u719f\u5f62\u5f0f\u7684\u4eba EGF (NP_001954.2) (Asn 971-Arg 1023) \u7684\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u5728 N \u672b\u7aef\u5177\u6709\u521d\u59cb Met\u3002\u4f7f\u7528 Balb\/C 3T3 \u5c0f\u9f20\u80da\u80ce\u6210\u7e96\u7dad\u7d30\u80de\u5728\u7d30\u80de\u589e\u6b96\u8a66\u9a57\u4e2d\u6e2c\u91cf\u3002\u9019\u7a2e\u6548\u679c\u7684 ED50 \u901a\u5e38\u70ba 0.15-0.75 pg\/mL\u3002\u91cd\u7d44\u4ebaEGF\u753154\u500b\u6c28\u57fa\u9178\u7d44\u6210\uff0c\u5728\u9084\u539f\u689d\u4ef6\u4e0b\u5728SDS-PAGE\u4e2d\u4f30\u8a08\u7684\u5206\u5b50\u91cf\u70ba6.3 kDa\u3002SDS-PAGE \u6e2c\u5b9a > 92 %\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nHuman EGF protein<\/a><\/td>\n<\/tr>\n
Sequence<\/strong><\/td>\nAmino acid sequence derived from mature form of human EGF (NP_001954.2) (Asn 971-Arg 1023) was expressed and purified, with an initial Met at the N-terminus.<\/td>\n<\/tr>\n
Activity<\/strong><\/td>\nMeasured in a cell proliferation assay using Balb\/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 pg\/mL.<\/td>\n<\/tr>\n
Protein length<\/strong><\/td>\nThe recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.<\/td>\n<\/tr>\n
Preparation method<\/strong><\/td>\nE. coli<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n> 92 % as determined by SDS-PAGE<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\nEpidermal Growth Factor; HOMG4; URG; Pro-epidermal growth factor; EGF<\/td>\n<\/tr>\n
Formulation<\/strong><\/td>\nLyophilized from sterile PBS, pH 7.4<\/td>\n<\/tr>\n
Molecular weight<\/strong><\/td>\n6.3 kDa<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\nAlways centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human EGF protein in sterile ddH2O not less than 100\u00b5g\/ml, which can then be further diluted to other aqueous solutions.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nLyophilized Human EGF proteinshould be stored desiccated below -20\u00b0C. Upon reconstitution, the protein should be stored at 4\u00b0C between 2-7 days and for future use below -20\u00b0C. For long term storage it is recommended to add a carrier protein (0.52% HSA or BSA). Please prevent freeze-thaw cycles.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nThe product is shipped at ambient temperature.<\/td>\n<\/tr>\n
Precautions<\/strong><\/td>\nThe product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u7d30\u80de\u6fc0\u7d20(Cytokines)-Human EGF protein\u91cd\u7d44\u86cb\u767d \u7d30\u80de\u6fc0\u7d20(Cytokines)\u662f\u4e00 […]<\/p>\n","protected":false},"author":3,"featured_media":29551,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5262],"tags":[869],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32556"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=32556"}],"version-history":[{"count":7,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32556\/revisions"}],"predecessor-version":[{"id":47551,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/32556\/revisions\/47551"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/29551"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=32556"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=32556"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=32556"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}