{"id":31778,"date":"2022-04-06T16:37:07","date_gmt":"2022-04-06T08:37:07","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31778"},"modified":"2022-10-21T10:25:58","modified_gmt":"2022-10-21T02:25:58","slug":"biothema%e5%8f%b0%e7%81%a3%e4%bb%a3%e7%90%86%e5%95%86","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31778","title":{"rendered":"BioThema\u53f0\u7063\u4ee3\u7406\u5546"},"content":{"rendered":"<p><span style=\"color: #008080;\"><a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=15166\"><strong>\u00a0\u7d30\u80de\u6d3b\u529b\u8a66\u5291\u76d2<\/strong><\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=15246\">ATP\u5f62\u6210\u8207\u964d\u89e3\u6e2c\u91cf<\/a>\u00a0|\u00a0<a style=\"color: #008080;\" href=\"http:\/\/biopioneer.com.tw\/?p=6698\"><strong>\u5fae\u751f\u7269<\/strong><strong>ATP<\/strong><strong>\u6aa2\u6e2c<\/strong><strong>\u5957\u7d44<\/strong><\/a>\u00a0<\/span><\/p>\n<p style=\"text-align: justify;\"><img decoding=\"async\" loading=\"lazy\" class=\"aligncenter size-full wp-image-2692\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg\" alt=\"biothema_header\" width=\"676\" height=\"100\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header.jpg 676w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2013\/12\/biothema_header-300x44.jpg 300w\" sizes=\"(max-width: 676px) 100vw, 676px\" \/><\/p>\n<p style=\"text-align: justify;\"><span style=\"color: #666699;\"><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%93%81%E7%89%8C%E4%BB%8B%E7%B4%B9-300x144.png\" alt=\"\u54c1\u724c\u4ecb\u7d39\" width=\"111\" height=\"53\" \/>\u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc\u745e\u5178\uff0c\u5c08\u9580\u7814\u767c\u751f\u7522\u51b7\u5149\uff08Bioluminescence\uff09\u4e4b\u76f8\u95dc\u7522\u54c1\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u3001\u9ad8\u7a69\u5b9a\u6027\u4e4bATP\u8207\u51b7\u5149\u5206\u6790\u8a66\u5291\u5957\u7d44\u3002\u5176\u61c9\u7528\u5305\u62ec\u5feb\u901f\u7d30\u80de\u8a08\u6578\u3001\u7d30\u80de\u589e\u751f\u8207\u6bd2\u6027\u3001\u514d\u75ab\u8a66\u9a57\u3001\u9175\u7d20\u8207\u4ee3\u8b1d\u7269\u76e3\u63a7\u3001Luciferase reporter geneassay\u3001\u74b0\u5883\u885b\u751f\u6aa2\u6e2c\u7b49\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000ATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u662f\u4e00\u7a2e\u5feb\u901f\u6aa2\u6e2c\u65b9\u6cd5\uff0c\u7528\u4ee5\u6aa2\u6e2c\u751f\u7269ATP\u7684\u542b\u91cf\u3002\u6b64\u65b9\u6cd5\u70ba\u7f8e\u570b\u592a\u7a7a\u7e3d\u7f72\u65bc1960\u5e74\u4ee3\u767c\u5c55\u51fa\u4f86\u7684\u6280\u8853\uff0c\u7576\u521d\u767c\u5c55\u6b64\u9805\u6280\u8853\u7684\u76ee\u7684\u662f\u5e0c\u671b\u80fd\u7528\u4ee5\u6aa2\u6e2c\u5916\u592a\u7a7a\u662f\u5426\u6709\u751f\u547d\u7269\u8cea\u5b58\u5728\uff0c\u5176\u53cd\u61c9\u539f\u7406\u8207\u87a2\u706b\u87f2\u9ad4\u5167\u767c\u5149\u53cd\u61c9\u76f8\u540c\uff0c\u5728\u87a2\u5149\u7d20\uff08luciferin\uff09\u8207\u87a2\u5149\u9175\u7d20\uff08luciferase\uff09\u5b58\u5728\u4e0b\uff0cATP\u8207luciferin\u53caluciferase\u50ac\u5316\u4e0b\u7522\u751f\u6de1\u85cd\u8272\u7684\u5149\u3002\u53cd\u61c9\u65b9\u7a0b\u5f0f\u5982\u4e0b\uff1a<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-607 aligncenter\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula.png\" alt=\"rapid_ATP_formula\" width=\"500\" height=\"33\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula.png 500w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP_formula-300x19.png 300w\" sizes=\"(max-width: 500px) 100vw, 500px\" \/><\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u8fd1\u4f86\u751f\u7269ATP\u51b7\u5149\u53cd\u61c9\u5728\u98df\u54c1\u8207\u91ab\u85e5\u9818\u57df\u4e2d\u88ab\u5ee3\u6cdb\u4f7f\u7528\u3002\u4ee5\u539f\u6838\u751f\u7269\u800c\u8a00\uff0c\u4e00\u96bb\u7d30\u83cc\u9ad4\u5167\u7d04\u542b2 x 10<sup>-18<\/sup>\u00a0mol ATP\uff1b\u771f\u83cc\u7d30\u80de\u5247\u542b10<sup>-15<\/sup>\uff5e10<sup>-16<\/sup>\u00a0mol ATP\u3002\u61c9\u7528ATP\u51b7\u5149\u53cd\u61c9\u6280\u8853\u6aa2\u6e2c\u751f\u83cc\u6578\u6642\uff0c\u9700\u5148\u5c07\u98df\u54c1\u4e2d\u975e\u5fae\u751f\u7269\u7684ATP\u6c34\u89e3\uff0c\u518d\u5c07\u5fae\u751f\u7269\u9ad4\u5167\u7684ATP\u91cb\u653e\u51fa\u4f86\uff0c\u53cd\u61c9\u5f8c\u4ee5ATP\u751f\u7269\u51b7\u5149\u5100\u5075\u6e2c\u3002\u82e5\u6aa2\u6e2c\u98df\u54c1\u63a5\u89f8\u8868\u9762\u7684\u6e05\u6f54\u5ea6\uff0c\u5247\u7121\u9700\u53bb\u9664\u98df\u54c1\u4e2d\u7684ATP\uff0c\u76f4\u63a5\u6e2c\u91cf\u5373\u53ef\u3002\u6aa2\u6e2c\u7d50\u679c\u5247\u4ee5\u76f8\u5c0d\u5438\u5149\u503c\uff08relative light unit, RLU\uff09\u7684\u591a\u5be1\u4f86\u8868\u793aATP\u6fc3\u5ea6\u7684\u9ad8\u4f4e\uff0c\u85c9\u6b64\u53cd\u63a8\u5fae\u751f\u7269\u542b\u91cf\u548c\u53ef\u80fd\u7684\u98df\u7269\u6b98\u7559\uff0c\u4ee5\u8868\u793a\u98df\u7269\u63a5\u89f8\u9762\u6e05\u6f54\u5ea6\u7684\u60c5\u6cc1\u3002<\/p>\n<p style=\"text-align: justify;\">\u3000\u3000\u76ee\u524dATP\u751f\u7269\u51b7\u5149\u53cd\u61c9\u6280\u8853\u61c9\u7528\u5728\u5404\u9805\u98df\u54c1\u7522\u696d\uff0c\u5305\u62ec\u751f\u4e73\u751f\u83cc\u6578\u4f30\u7b97\u3001\u74b0\u5883\u8a2d\u5099\u6e05\u6f54\u8a55\u4f30\u3001\u755c\u7522\u696d\u4e2d\u5c60\u9ad4\u7684\u6c61\u67d3\u8a55\u4f30\u3001\u679c\u6c41\u7522\u696d\u7684\u751f\u83cc\u6578\u4f30\u7b97\u3001\u88fd\u9152\u5de5\u696d\u53ca\u98f2\u6599\u696d\u7b49\u3002<\/p>\n<p style=\"text-align: justify;\"><a href=\"https:\/\/biothema.com\/\"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E5%8E%9F%E5%BB%A0%E9%80%A3%E7%B5%9001-300x79.png\" alt=\"\u539f\u5ee0\u9023\u7d5001\" width=\"138\" height=\"36\" \/><\/a><\/p>\n<p><span style=\"font-size: medium; color: #008000;\">\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf\u5957\u7d44\u7522\u54c1\u5206\u985e\uff1a<\/span><\/p>\n<p style=\"text-align: center;\"><a href=\"http:\/\/biothema.se\/wp-content\/uploads\/2011\/05\/Product-selection-guide-WEB-2012.pdf\" target=\"_blank\"><img decoding=\"async\" loading=\"lazy\" class=\"size-full wp-image-606 aligncenter\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP.png\" alt=\"rapid_atp_chart\" width=\"671\" height=\"266\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP.png 671w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/01\/rapid_ATP-300x118.png 300w\" sizes=\"(max-width: 671px) 100vw, 671px\" \/><\/a><\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\" align=\"center\">\n<tbody>\n<tr>\n<td width=\"100\">\n<p align=\"center\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/p>\n<\/td>\n<td width=\"476\">\n<p align=\"center\"><strong>\u54c1\u540d<\/strong><\/p>\n<\/td>\n<td width=\"100\">\n<p align=\"center\"><strong>\u898f\u683c<\/strong><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-112<\/p>\n<\/td>\n<td width=\"476\">Microbial ATP Kit HS<\/td>\n<td nowrap=\"nowrap\" width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-111<\/p>\n<\/td>\n<td width=\"476\">Intracellular ATP Kit HS<\/td>\n<td width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">\n<p align=\"center\">266-311<\/p>\n<\/td>\n<td valign=\"center\" width=\"476\">ATP Biomass Kit HS<\/td>\n<td width=\"100\">\n<p align=\"center\">375 test<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #00ccff;\"><a style=\"color: #00ccff;\" href=\"http:\/\/biopioneer.com.tw\/?p=6698\"><strong style=\"color: #00ccff;\">Microbial ATP Kit HS, 266-112\u00a0<\/strong><strong style=\"color: #00ccff;\">\u5fae\u751f\u7269<\/strong><strong style=\"color: #00ccff;\">ATP<\/strong><strong style=\"color: #00ccff;\">\u6aa2\u6e2c<\/strong><strong style=\"color: #00ccff;\">\u5957\u7d44<\/strong><\/a>\u00a0\u53ef\u9078\u64c7\u5fae\u5b54\u677f\u548c cuvette \u65b9\u6cd5\u6e2c\u91cf<\/span><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E7%94%A2%E5%93%81%E4%BB%8B%E7%B4%B9-300x144.png\" alt=\"\u7522\u54c1\u4ecb\u7d39\" width=\"109\" height=\"53\" \/>\u5fae\u751f\u7269\u7684ATP\u8a66\u5291\u76d2 (\u8ca8\u865f\u8a8c<strong>266-112)<\/strong>\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u83ccATP\u00a0(adenosine triphosphate)\u7684\u542b\u91cf\u3002\u7576\u6aa2\u9ad4\u4f86\u81ea\u9ad4\u6db2\u3001\u553e\u6db2\u7b49\uff0c\u53ef\u7d93\u7531\u5957\u7d44\u5167\u7684ATP\u6d88\u9664\u8a66\u5291\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\u4f86\u6e90\u3002\u7d30\u83cc\u611f\u67d3\u4eba\u9ad4\u5f8c\uff0c\u5f88\u96e3\u7d93\u7531\u5168\u90e8\u6a23\u54c1\u4e2d\uff0c\u76f4\u63a5\u6e2c\u91cf\u7d30\u83cc\u7684\u7d30\u80de\u6578\u3002\u56e0\u6b64\u5fc5\u9808\u5728\u9032\u884c\u6e2c\u91cf\u7d30\u83cc\u7d30\u80de\u6578\u524d\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\uff1b\u6700\u5f8c\u771f\u5be6\u6e2c\u91cf\u7d30\u83cc\u672c\u8eab\u4e4bATP\u542b\u91cf\u3002<img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/biothema.com\/wp-content\/uploads\/2016\/06\/DSC00437.jpg\" alt=\"\" width=\"515\" height=\"368\" \/><\/p>\n<p><strong>\u5fae\u751f\u7269<\/strong><strong>ATP<\/strong><strong>\u6aa2\u6e2c<\/strong><strong>\u5957\u7d44<\/strong><strong>\u00a0|\u00a0<\/strong><strong>\u7522\u54c1\u7279\u9ede<\/strong><\/p>\n<ol>\n<li>2&#215;10<sup>-18<\/sup>\u00a0mole ATP per cell<\/li>\n<li>\u79fb\u9664\u7d30\u83cc\u5916ATP\uff0c\u6e2c\u91cf\u771f\u6b63\u4e4b\u7d30\u83cc\u6578<\/li>\n<li>ATP\u8a66\u5291\u7684\u6539\u826f\uff0c\u6bcf\u52066%\u7684\u901f\u5ea6\u8870\u6e1b<\/li>\n<li>ATP\u5206\u6790\u7684\u6821\u6b63-\u4e0d\u9808Standard curve<\/li>\n<li>15\u5206\u9418\u5167\u5b8c\u6210ATP\u6d3b\u6027\u5206\u6790<\/li>\n<\/ol>\n<p>Biothema AB\u6210\u7acb1987\u5e74\uff0c\u662f\u4e16\u754c\u4e0a\u5c08\u696d\u5229\u7528\u87a2\u706b\u87f2\u9176 (Luciferase) \u6280\u8853\u9032\u884c\u7d30\u80de\u3001\u57fa\u56e0\u3001\u9176\u8207\u65b0\u9673\u4ee3\u8b1d\u7814\u7a76\u7684\u9818\u5148\u8005\u3002Biotherma\u516c\u53f8\u5275\u59cb\u4eba<em>Arne Lundin<\/em>\u00a0\u535a\u58eb\u662f\u6b64\u9818\u57df\u7684\u570b\u969b\u6b0a\u5a01\u4eba\u7269\u3002<a href=\"http:\/\/www.biothema.com\/\">www.biothema.com<\/a>\u00a0Biotherma\u6838\u5fc3\u6280\u8853Biotherma\u57fa\u65bc\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176Luciferase\u6280\u8853\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u7684ATP\u8a66\u5291\uff0c\u5176\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u53ca\u4f7f\u7528\u7c21\u55ae\u3002<\/p>\n<p><a href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2019\/05\/266-112-Microbial-ATP-Kit-HS.pdf \"><img decoding=\"async\" loading=\"lazy\" class=\"alignnone\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E6%93%8D%E4%BD%9C%E6%89%8B%E5%86%8A-300x144.png\" alt=\"\u64cd\u4f5c\u624b\u518a\" width=\"108\" height=\"53\" \/><\/a><\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"100\"><strong>\u7522\u54c1\u7de8\u865f<\/strong><\/td>\n<td width=\"476\"><strong>\u7522\u54c1\u540d\u7a31<\/strong><\/td>\n<td width=\"100\"><strong>\u898f\u683c<\/strong><\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>ATP<\/strong><strong>\u751f\u6210\u4ee3\u8b1d<\/strong><strong>\u00a0\u00a0<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">111-021<\/td>\n<td width=\"476\">Kinase RR Kit\uff0d\u5075\u6e2cKinase\u6216aminoacyl tRNA\u5408\u6210\u9176\u53ca\u76f8\u95dc\u53d7\u8cea<\/td>\n<td width=\"100\">1000 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">144-041<\/td>\n<td width=\"476\">ATP Kit SL\uff0d\u5075\u6e2cATP\u751f\u6210\u8207\u964d\u89e3<\/td>\n<td width=\"100\">1000 tests<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>\u5feb\u901f\u7d30\u80de\u8a08\u6578\u8207\u7d30\u80de\u6bd2\u6027\u5206\u6790<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">155-050<\/td>\n<td width=\"476\">Cellular ATP Kit HTS\uff0d\u5075\u6e2cMammalian cells\u4e14\u4f7f\u7528\u5177\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td width=\"100\">1200 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">188-441<\/td>\n<td width=\"476\">Cell Viability Kit SL\uff0d\u5075\u6e2cMammalian cells\u4e14\u4f7f\u7528\u7121\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td width=\"100\">1600 tests<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><a href=\"http:\/\/biopioneer.com.tw\/?p=153\"><strong>\u5fae\u751f\u7269\u5feb\u901f\u5b9a\u91cf<\/strong><\/a><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">266-111<\/td>\n<td width=\"476\">Intracellular ATP Kit HS\uff0d\u5075\u6e2c\u542b\u6709Mammalian cells\u53ca\u542b\u6709\u7d30\u80de\u5916ATP\u4e4b\u5fae\u751f\u7269<\/td>\n<td width=\"100\">375 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">266-112<\/td>\n<td width=\"476\">Microbial ATP Kit HS\uff0d\u5075\u6e2c\u7121Mammalian cells\u4f46\u542b\u6709\u7d30\u80de\u5916ATP\u4e4b\u5fae\u751f\u7269<\/td>\n<td width=\"100\">375 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">266-311<\/td>\n<td width=\"476\">ATP Biomass Kit HS\uff0d\u5075\u6e2c\u7121Mammalian cells\u53ca\u7d30\u80de\u5916ATP\u4e4b\u5fae\u751f\u7269<\/td>\n<td width=\"100\">375 tests<\/td>\n<\/tr>\n<tr>\n<td colspan=\"3\" width=\"680\"><strong>Luciferase reporter gene assay<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"100\">484-001<\/td>\n<td width=\"476\">Luciferase Assay Kit<\/td>\n<td width=\"100\">800 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">484-102<\/td>\n<td width=\"476\">Luciferase Assay Kit HTS\uff0d\u9069\u5408\u5927\u91cf\u5206\u6790\u4e14\u7121\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td width=\"100\">4800 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\">484-103<\/td>\n<td width=\"476\">Luciferase Assay Kit HTS\uff0d\u9069\u5408\u5927\u91cf\u5206\u6790\u4e14\u5177\u5206\u6ce8\u5668\u4e4b\u51b7\u5149\u5100<\/td>\n<td width=\"100\">4600 tests<\/td>\n<\/tr>\n<tr>\n<td width=\"100\"><\/td>\n<td width=\"476\"><\/td>\n<td width=\"100\"><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/10\/266-111-Intracellular-ATP-Kit.pdf\">Intracellular ATP Kit HS (266-111)\u64cd\u4f5c\u624b\u518a\u4e0b\u8f09<\/a><\/span><\/p>\n<p><span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/10\/266-112-Microbial-ATP-Kit.pdf\">Microbial ATP Kit HS\u00a0(266-112)\u64cd\u4f5c\u624b\u518a\u4e0b\u8f09<\/a><\/span><\/p>\n<p><span style=\"color: #ff0000;\"><a style=\"color: #ff0000;\" href=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/downloads\/2022\/10\/266-311-TP-Biomass-Kit.pdf\">ATP Biomass Kit HS\u00a0(266-311)\u64cd\u4f5c\u624b\u518a\u4e0b\u8f09<\/a><\/span><\/p>\n<p><strong>\u5fae\u751f\u7269<\/strong><strong>ATP<\/strong><strong>\u6aa2\u6e2c\u6280\u8853<\/strong><strong>\u00a0(Microbial ATP Kit HS, 266-112)<\/strong><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2022\/03\/%E7%94%A2%E5%93%81%E5%8E%9F%E7%90%86-300x144.png\" alt=\"\u7522\u54c1\u539f\u7406\" width=\"114\" height=\"55\" \/>ATP\u542b\u91cf\u5728\u6240\u6709\u7684\u6d3b\u7d30\u80de\u4e2d\u662f\u76f8\u4f3c\u7684\uff0c\u6bcf\u500b\u7d30\u80de\u4e2d\u7684ATP\u542b\u91cf\u8207\u7d30\u80de\u5927\u5c0f\u6210\u6bd4\u4f8b\u76f8\u95dc\u3002\u5927\u90e8\u4efd\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u5927\u7d04\u5177\u67092&#215;10<sup>-18<\/sup>\u00a0mole ATP per cell\u3002\u771f\u6838\u7d30\u80de\u6bd4\u7d30\u83cc\u5927\uff0c\u5927\u7d04\u542b\u670910<sup>-15 mole ATP\u3002<\/sup><\/p>\n<p><sup><strong>Biothema<\/strong><strong>\u51b7\u5149\u8a66\u5291\u9748\u654f\u5ea6\u6700\u9ad8<\/strong><\/sup><\/p>\n<p>Biothema\u63d0\u4f9b\u5e02\u9762\u4e0a\u6700\u9ad8\u9748\u654f\u5ea6\u7684ATP\u8a66\u5291\uff0c\u53ef\u4ee5\u6aa2\u6e2cSensitivity10<sup>-17<\/sup>mole ATP\u3002\u5927\u90e8\u4efd\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u5927\u7d04\u5177\u67092&#215;10<sup>-18<\/sup>\u00a0mole ATP per cell\u3002\u771f\u6838\u7d30\u80de\u6bd4\u7d30\u83cc\u5927\uff0c\u5927\u7d04\u542b\u670910<sup>-15<\/sup>\u00a0mole ATP\u3002\u56e0\u6b64\u6aa2\u9ad4\u5167\u82e5\u542b\u67095\u500b\u7d30\u83cc\u7d30\u80de\uff0c\u5373\u53ef\u6e2c\u5f97\u5176ATP\u542b\u91cf\u3002<\/p>\n<p><strong>Biothema<\/strong><strong>\u79fb\u9664\u7d30\u83cc\u5916<\/strong><strong>ATP<\/strong><strong>\uff0c\u6e2c\u91cf\u771f\u6b63\u7d30\u83cc\u4e4b\u7d30\u80de\u6578<\/strong><\/p>\n<p>\u5728\u904e\u53bb30\u5e74\u4e2d\uff0c\u87a2\u5149\u7d20\u9176ATP\u5206\u6790\u7684\u9748\u654f\u5ea6\u53ea\u5c40\u9650\u65bc10<sup>-14<\/sup>\u3002\u73fe\u5728\u63d0\u9ad8\u63a5\u8fd110<sup>-18<\/sup>\u00a0mole ATP \u3002\u56e0\u6b64\u66f4\u5ee3\u6cdb\u61c9\u7528\u65bc\u98df\u54c1\u5de5\u5ee0\u3001\u5fae\u751f\u7269\u63a7\u5236\u3001\u771f\u6838\u7d30\u80de\u8207\u539f\u6838\u7d30\u80de\u6d3b\u6027\u5206\u6027\u3002\u7d30\u83cc\u611f\u67d3\u4eba\u9ad4\u5f8c\uff0c\u5f88\u96e3\u7d93\u7531\u5168\u90e8\u6a23\u54c1\u4e2d\uff0c\u76f4\u63a5\u6e2c\u91cf\u7d30\u83cc\u7684\u7d30\u80de\u6578\u3002\u56e0\u6b64\u5fc5\u9808\u5728\u9032\u884c\u6e2c\u91cf\u7d30\u83cc\u7d30\u80de\u6578\u524d\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\uff0c\u4f8b\u5982\u9ad4\u6db2\uff0c\u8840\u6db2\uff0c\u553e\u6db2\u7b49\u7b49\u3002<\/p>\n<p><strong>ATP<\/strong><strong>\u8a66\u5291\u7684\u6539\u826f\uff0c\u6bcf\u5206<\/strong><strong>10%<\/strong><strong>\u7684\u901f\u5ea6\u8870\u6e1b<\/strong><\/p>\n<p>Biothema\u516c\u53f820\u591a\u5e74\u65bcATP\u51b7\u5149\u8a66\u5291\u4e4b\u4e0d\u65b7\u7814\u7a76\u8207\u7d93\u9a57\u7d2f\u7a4d\u4e0b\uff0c\u63d0\u4f9b\u6700\u4f73\u5316ATP\u51b7\u5149\u8a66\u5291\u3002\u5176\u767c\u5149\u6548\u7387\u4ee5\u6bcf\u5206\u937e10%\u7684\u901f\u5ea6\u8870\u6e1b\u3002\u56e0\u6b64\u5373\u4f7f\u662f\u624b\u52d5\u64cd\u4f5c\uff0c\u4e5f\u63d0\u4f9b\u826f\u597d\u7684\u6e96\u78ba\u6027\u3002\u800c\u6b64ATP\u8a66\u5291\u7684\u80cc\u666f\u503c\u4f4e\u65bc\u4e0d\u53ef\u6aa2\u6e2c\u7684\u6c34\u5e73\u7bc4\u570d (\u5c0f\u65bc10<sup>-18<\/sup>\/\u6aa2\u6e2c)\u3002<\/p>\n<p><strong>ATP<\/strong><strong>\u5206\u6790\u7684\u6821\u6b63<\/strong><strong>&#8211;<\/strong><strong>\u4e0d\u9808<\/strong><strong>Standard curve<\/strong><\/p>\n<p>ATP\u5206\u6790\u5fc5\u9808\u901a\u5167\u5728ATP\u6a19\u6e96\u54c1\u4f86\u9032\u884c\u6821\u6b63\u3002\u7368\u7279\u5167\u6821\u5f0fATP\u6a19\u6e96\u54c1\uff0c\u4e0d\u9700\u53e6\u4f5c\u6a19\u6e96\u66f2\u7dda\u5373\u53ef\u5feb\u901f\u5075\u6e2c\u6a23\u54c1ATP\u542b\u91cf\uff0c\u5176\u6e2c\u5f97\u7684\u6578\u503c\u70ba\u7d55\u5c0d\u55ae\u4f4d(mole)\uff0c\u975e\u4e00\u822c\u76f8\u5c0d\u55ae\u4f4d(RLU)<\/p>\n<p><strong>15<\/strong><strong>\u5206\u9418\u5167\u5b8c\u6210<\/strong><strong>ATP<\/strong><strong>\u6d3b\u6027\u5206\u6790<\/strong><\/p>\n<p>\u7d30\u83cc\u5916ATP\u4e4b\u79fb\u9664\u8207\u964d\u89e3\u904e\u7a0b\u5927\u7d0410\u5206\u9418\uff0c\u5176\u65bc\u6b65\u9a5f\u52a0\u5b8c\u5373\u5b8c\u6210\u3002\u5168\u90e8\u6d41\u7a0b\u5927\u7d0415\u5206\u5167\u5b8c\u6210\u3002<\/p>\n<p>\u64cd\u4f5c\u65b9\u6cd5:\u00a0 \u53d620\uff0850\uff09\u00b5L\u6a23\u54c1\u8207\u7b49\u9ad4\u7a4dATP\u6d88\u9664\u8a66\u5291(ATP Eliminating Reagent) \u6df7\u5408\uff0c\u5ba4\u6eab10\u5206\u9418\u3002<\/p>\n<p>\u52a0\u516520\uff0850\uff09\u00b5L Extractant B\/S<\/p>\n<p>\u52a0\u5165160\uff08400\uff09\u00b5L ATP Reagent HS, \u4e26\u6e2c\u91cf\u51b7\u5149 Ismp<\/p>\n<p>\u52a0\u516510\uff0810\uff09\u00b5L 100 nmol \/ L ATP\u6a19\u6e96\u6db2\uff0c\u53731pmol ATP\uff0c\u6e2c\u91cf\u51b7\u5149Ismp + std<\/p>\n<p><strong>Microbial ATP KIT HS, 266-112<\/strong><strong>\u7522\u54c1\u7279\u9ede\u6574\u7406<\/strong><\/p>\n<p><em>Fast Assay: Results within minutes Highly Sensitive: 10<sup>-17<\/sup>\u00a0mol ATP (~ 5 bacteria) Reliable: Stable, Ready-to-use ATP Standard Cost-efficient: No Standard curve required Flexible: Choice of\u00a0\u03bc-plate &amp; cuvette method<\/em><\/p>\n<p><strong>Microbial ATP KIT HS, 266-112<\/strong><strong>\u7522\u54c1\u898f\u683c<\/strong><\/p>\n<p>\u4e00\u7d44ATP reagent HS\u8a66\u5291\u53ef\u4ee5\u505a150~375\u6b21\u3002\u6bcf\u4e00\u5c0f\u7ba1\u53ef\u9032\u884c25\u500bTube luminometer\u53ca62.5\u500bmicroplate luminometer\u3002\u6574\u7d44kit\u5305\u62ec\u67096\u5c0f\u7ba1ATP reagent HS\uff0c\u6240\u4ee5\u53ef\u9032\u884c150\u500bTube luminometer\u53ca375\u500bmicroplate \u3002luminometer\u3002<\/p>\n<ol>\n<li>ATP Reagent HS (6Vials)\u51cd\u4e7e\u578b\u5f0f\uff1b\u542b\u6709\u87a2\u5149\u7d20\u9176(luciferase) \u548c\u87a2\u5149\u7d20( luciferin) \u4f7f\u7528\u524d\u9700\u8207\u00a0Diluent B\u6eb6\u6db2\u91cd\u7d44<\/li>\n<li>Diluent B 10ml (6 vials)\u91cd\u7d44\u8a66\u5291\uff1bBuffer used to reconstitute ATP Reagent HS.\u00a0\u91cd\u7d44\u8a66\u5291\u4e2d\u7684\u87a2\u5149\u7d20\u9176\u6d3b\u6027\u00a0 \u00a0Diluent B 10 mL (6 vials)<\/li>\n<li>Extractant B\/S 10ml, (1 vial) \u7d30\u80deATP\u8403\u53d6\u6db2: \u91cb\u653e\u7d30\u80de\u5167ATP<\/li>\n<li>ATP standard 5ml (1 vial), contains 10<sup>-7<\/sup>\u00a0mol\/L ATP<\/li>\n<li>ATP Eliminating Reagent (1 vial) \u4f7f\u7528\u524d\u9700\u8207Cell lysing Reagent\u91cd\u7d44\u3002<\/li>\n<li>Cell lysing Reagent\u91cd\u7d44\u8a66\u529110ml (1 vial). Used to reconstitute ATP Eliminating Reagnent.<\/li>\n<\/ol>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"wp-image-2678 size-full\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification.jpg\" alt=\"Biothema \u7522\u54c1\u61c9\u7528\u5206\u985e\" width=\"400\" height=\"330\" srcset=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification.jpg 400w, http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2014\/05\/Biothema_classification-300x247.jpg 300w\" sizes=\"(max-width: 400px) 100vw, 400px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u00a0\u7d30\u80de\u6d3b\u529b\u8a66\u5291\u76d2\u00a0|\u00a0ATP\u5f62\u6210\u8207\u964d\u89e3\u6e2c\u91cf\u00a0|\u00a0\u5fae\u751f\u7269ATP\u6aa2\u6e2c\u5957\u7d44\u00a0 \u3000\u3000BioThema\u65bc2001\u5e74\u5efa\u7acb\u65bc [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":2680,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[760],"tags":[911],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31778"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31778"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31778\/revisions"}],"predecessor-version":[{"id":34939,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31778\/revisions\/34939"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/2680"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31778"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31778"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31778"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}