{"id":31775,"date":"2022-04-06T16:35:12","date_gmt":"2022-04-06T08:35:12","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31775"},"modified":"2022-07-19T11:41:36","modified_gmt":"2022-07-19T03:41:36","slug":"atp%e9%99%8d%e8%a7%a3%e6%ba%b6%e6%b6%b2-atp-eliminating-reagent","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31775","title":{"rendered":"ATP\u964d\u89e3\u6eb6\u6db2 | ATP Eliminating Reagent"},"content":{"rendered":"

ATP Eliminating Reagent-ATP degrading enzyme<\/a><\/span><\/p>\n

Depending on with diluent is used\u00a0when reconstitution ATP Eliminating Enzyme it is either used for degrading mammalian ATP and\u00a0measuring\u00a0bacterial ATP\u00a0or for degrading extracellular ATP and measuring mammalian cells.<\/p>\n

\"\"<\/p>\n

\u7522\u54c1\u4ecb\u7d39: ATP Elimination Reagent \u7528\u65bc\u5feb\u901f\u964d\u89e3\u6a23\u54c1\u4e2d\u7684\u7d30\u80de\u5916 ATP (extracellular ATP)\u3002 ATP \u6fc3\u5ea6\u4ee5\u6bcf\u79d2 50% \u7684\u901f\u5ea6\u964d\u4f4e\u3002\u5c0d\u65bc\u751f\u7269\u6a23\u54c1\uff0c\u8acb\u7b49\u5f85 10 \u5206\u9418\u4ee5\u5b8c\u5168\u964d\u89e3\u7d30\u80de\u5916 ATP\u3002ATP \u6d88\u9664\u8a66\u5291\u61c9\u5728\u7a00\u91cb\u5291 C (Diluent C, \u8ca8\u865f23-101)\u6216\u7d30\u80de\u88c2\u89e3\u8a66\u5291 (Cell Lysing Reagent, \u8ca8\u865f28-101)\u4e2d\u5fa9\u6eb6\uff0c\u5982\u679c\u54fa\u4e73\u52d5\u7269\u7d30\u80de\u61c9\u540c\u6642\u6d88\u9664\u3002<\/p>\n

\u7d30\u80de\u6d3b\u529b\u8a66\u5291\u76d2<\/strong><\/a>\u00a0|\u00a0ATP\u5f62\u6210\u8207\u964d\u89e3\u6e2c\u91cf<\/a>\u00a0|\u00a0\u5fae\u751f\u7269<\/strong>ATP<\/strong>\u6aa2\u6e2c\u5957\u7d44<\/strong><\/a><\/p>\n

Microbial ATP Kit HS, 266-112 <\/strong>\u5fae\u751f\u7269<\/strong>ATP<\/strong>\u6aa2\u6e2c<\/strong>\u5957\u7d44<\/strong><\/p>\n

\u5fae\u751f\u7269\u7684ATP\u8a66\u5291\u76d2 (\u8ca8\u865f\u8a8c266-112)<\/strong>\uff0c\u7528\u65bc\u6aa2\u6e2c\u7d30\u83cc\u7d30\u80de\u4e2dATP\u7684\u542b\u91cf\u3002\u7576\u6aa2\u9ad4\u4f86\u81ea\u9ad4\u6db2\u3001\u553e\u6db2\u7b49\uff0c\u53ef\u7d93\u7531\u5957\u7d44\u5167\u7684ATP\u6d88\u9664\u8a66\u5291\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\u4f86\u6e90\u3002\u7d30\u83cc\u611f\u67d3\u4eba\u9ad4\u5f8c\uff0c\u5f88\u96e3\u7d93\u7531\u5168\u90e8\u6a23\u54c1\u4e2d\uff0c\u76f4\u63a5\u6e2c\u91cf\u7d30\u83cc\u7684\u7d30\u80de\u6578\u3002\u56e0\u6b64\u5fc5\u9808\u5728\u9032\u884c\u6e2c\u91cf\u7d30\u83cc\u7d30\u80de\u6578\u524d\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\uff0c\u4f8b\u5982\u9ad4\u6db2\uff0c\u8840\u6db2\uff0c\u553e\u6db2\u7b49\u7b49\uff1b\u6700\u5f8c\u771f\u5be6\u6e2c\u91cf\u7d30\u83cc\u672c\u8eab\u4e4bATP\u542b\u91cf\u3002<\/p>\n

\u5fae\u751f\u7269<\/strong>ATP<\/strong>\u6aa2\u6e2c<\/strong>\u5957\u7d44<\/strong> | <\/strong>\u7522\u54c1\u7279\u9ede<\/strong><\/p>\n

    \n
  1. 2×10-18 mole ATP per cell<\/li>\n
  2. \u79fb\u9664\u7d30\u83cc\u5916ATP\uff0c\u6e2c\u91cf\u771f\u6b63\u4e4b\u7d30\u83cc\u6578<\/li>\n
  3. ATP\u8a66\u5291\u7684\u6539\u826f\uff0c\u6bcf\u52066%\u7684\u901f\u5ea6\u8870\u6e1b<\/li>\n
  4. ATP\u5206\u6790\u7684\u6821\u6b63-\u4e0d\u9808Standard curve<\/li>\n
  5. 15\u5206\u9418\u5167\u5b8c\u6210ATP\u6d3b\u6027\u5206\u6790<\/li>\n<\/ol>\n

    Biothema<\/strong>\u51b7\u5149\u8a66\u5291\u9748\u654f\u5ea6\u6700\u9ad8<\/strong><\/p>\n

    Biothema\u63d0\u4f9b\u5e02\u9762\u4e0a\u6700\u9ad8\u9748\u654f\u5ea6\u7684ATP\u8a66\u5291\uff0c\u53ef\u4ee5\u6aa2\u6e2cSensitivity10-17mole ATP\u3002\u5927\u90e8\u4efd\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u5927\u7d04\u5177\u67092×10-18 mole ATP per cell\u3002\u771f\u6838\u7d30\u80de\u6bd4\u7d30\u83cc\u5927\uff0c\u5927\u7d04\u542b\u670910-15 mole ATP\u3002\u56e0\u6b64\u6aa2\u9ad4\u5167\u82e5\u542b\u67095\u500b\u7d30\u83cc\u7d30\u80de\uff0c\u5373\u53ef\u6e2c\u5f97\u5176ATP\u542b\u91cf\u3002<\/p>\n

    \u200b<\/p>\n

    Biothema AB\u6210\u7acb1987\u5e74\uff0c\u662f\u4e16\u754c\u4e0a\u5c08\u696d\u5229\u7528\u87a2\u706b\u87f2\u9176 (Luciferase) \u6280\u8853\u9032\u884c\u7d30\u80de\u3001\u57fa\u56e0\u3001\u9176\u8207\u65b0\u9673\u4ee3\u8b1d\u7814\u7a76\u7684\u9818\u5148\u8005\u3002Biotherma\u516c\u53f8\u5275\u59cb\u4ebaArne Lundin<\/em>\u00a0\u535a\u58eb\u662f\u6b64\u9818\u57df\u7684\u570b\u969b\u6b0a\u5a01\u4eba\u7269\u3002www.biothema.com<\/a><\/p>\n

    Biotherma\u6838\u5fc3\u6280\u8853<\/p>\n

    Biotherma\u57fa\u65bc\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176Luciferase\u6280\u8853\uff0c\u63d0\u4f9b\u9ad8\u54c1\u8cea\u7684ATP\u8a66\u5291\uff0c\u5176\u5177\u6709\u9ad8\u9748\u654f\u5ea6\u53ca\u4f7f\u7528\u7c21\u55ae\u3002\u5176\u87a2\u5149\u7d20\u9176\u50ac\u5316\u4ee5\u4e0b\u4e4b\u53cd\u61c9\u3002<\/p>\n

    \u5fae\u751f\u7269<\/strong>ATP<\/strong>\u6aa2\u6e2c\u6280\u8853<\/strong> (Microbial ATP Kit HS, 266-112)<\/strong><\/p>\n

    \u5206\u6790\u539f\u7406: ATP\u542b\u91cf\u5728\u6240\u6709\u7684\u6d3b\u7d30\u80de\u4e2d\u662f\u76f8\u4f3c\u7684\uff0c\u6bcf\u500b\u7d30\u80de\u4e2d\u7684ATP\u542b\u91cf\u8207\u7d30\u80de\u5927\u5c0f\u6210\u6bd4\u4f8b\u76f8\u95dc\u3002\u5927\u90e8\u4efd\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u5927\u7d04\u5177\u67092×10-18 mole ATP per cell\u3002\u771f\u6838\u7d30\u80de\u6bd4\u7d30\u83cc\u5927\uff0c\u5927\u7d04\u542b\u670910-15 mole ATP\u3002<\/p>\n

    Biothema<\/strong>\u51b7\u5149\u8a66\u5291\u9748\u654f\u5ea6\u6700\u9ad8<\/strong><\/p>\n

    Biothema\u63d0\u4f9b\u5e02\u9762\u4e0a\u6700\u9ad8\u9748\u654f\u5ea6\u7684ATP\u8a66\u5291\uff0c\u53ef\u4ee5\u6aa2\u6e2cSensitivity10-17mole ATP\u3002\u5927\u90e8\u4efd\u7684\u7d30\u83cc\u7d30\u80de\uff0c\u5927\u7d04\u5177\u67092×10-18 mole ATP per cell\u3002\u771f\u6838\u7d30\u80de\u6bd4\u7d30\u83cc\u5927\uff0c\u5927\u7d04\u542b\u670910-15 mole ATP\u3002\u56e0\u6b64\u6aa2\u9ad4\u5167\u82e5\u542b\u67095\u500b\u7d30\u83cc\u7d30\u80de\uff0c\u5373\u53ef\u6e2c\u5f97\u5176ATP\u542b\u91cf\u3002<\/p>\n

    Biothema<\/strong>\u79fb\u9664\u7d30\u83cc\u5916<\/strong>ATP<\/strong>\uff0c\u6e2c\u91cf\u771f\u6b63\u7d30\u83cc\u4e4b\u7d30\u80de\u6578<\/strong><\/p>\n

    \u5728\u904e\u53bb30\u5e74\u4e2d\uff0c\u87a2\u5149\u7d20\u9176ATP\u5206\u6790\u7684\u9748\u654f\u5ea6\u53ea\u5c40\u9650\u65bc10-14\u3002\u73fe\u5728\u63d0\u9ad8\u63a5\u8fd110-18 mole ATP \u3002\u56e0\u6b64\u66f4\u5ee3\u6cdb\u61c9\u7528\u65bc\u98df\u54c1\u5de5\u5ee0\u3001\u5fae\u751f\u7269\u63a7\u5236\u3001\u771f\u6838\u7d30\u80de\u8207\u539f\u6838\u7d30\u80de\u6d3b\u6027\u5206\u6027\u3002\u7d30\u83cc\u611f\u67d3\u4eba\u9ad4\u5f8c\uff0c\u5f88\u96e3\u7d93\u7531\u5168\u90e8\u6a23\u54c1\u4e2d\uff0c\u76f4\u63a5\u6e2c\u91cf\u7d30\u83cc\u7684\u7d30\u80de\u6578\u3002\u56e0\u6b64\u5fc5\u9808\u5728\u9032\u884c\u6e2c\u91cf\u7d30\u83cc\u7d30\u80de\u6578\u524d\uff0c\u5148\u79fb\u9664\u6216\u964d\u89e3\u6389\u7d30\u83cc\u4ee5\u5916\u7684ATP\uff0c\u4f8b\u5982\u9ad4\u6db2\uff0c\u8840\u6db2\uff0c\u553e\u6db2\u7b49\u7b49\u3002<\/p>\n

    ATP<\/strong>\u8a66\u5291\u7684\u6539\u826f\uff0c\u6bcf\u5206<\/strong>10%<\/strong>\u7684\u901f\u5ea6\u8870\u6e1b<\/strong><\/p>\n

    Biothema\u516c\u53f820\u591a\u5e74\u65bcATP\u51b7\u5149\u8a66\u5291\u4e4b\u4e0d\u65b7\u7814\u7a76\u8207\u7d93\u9a57\u7d2f\u7a4d\u4e0b\uff0c\u63d0\u4f9b\u6700\u4f73\u5316ATP\u51b7\u5149\u8a66\u5291\u3002\u5176\u767c\u5149\u6548\u7387\u4ee5\u6bcf\u5206\u937e10%\u7684\u901f\u5ea6\u8870\u6e1b\u3002\u56e0\u6b64\u5373\u4f7f\u662f\u624b\u52d5\u64cd\u4f5c\uff0c\u4e5f\u63d0\u4f9b\u826f\u597d\u7684\u6e96\u78ba\u6027\u3002\u800c\u6b64ATP\u8a66\u5291\u7684\u80cc\u666f\u503c\u4f4e\u65bc\u4e0d\u53ef\u6aa2\u6e2c\u7684\u6c34\u5e73\u7bc4\u570d (\u5c0f\u65bc10-18\/\u6aa2\u6e2c)\u3002<\/p>\n

    ATP<\/strong>\u5206\u6790\u7684\u6821\u6b63<\/strong>–<\/strong>\u4e0d\u9808<\/strong>Standard curve<\/strong><\/p>\n

    ATP\u5206\u6790\u5fc5\u9808\u901a\u5167\u5728ATP\u6a19\u6e96\u54c1\u4f86\u9032\u884c\u6821\u6b63\u3002\u7368\u7279\u5167\u6821\u5f0fATP\u6a19\u6e96\u54c1\uff0c\u4e0d\u9700\u53e6\u4f5c\u6a19\u6e96\u66f2\u7dda\u5373\u53ef\u5feb\u901f\u5075\u6e2c\u6a23\u54c1ATP\u542b\u91cf\uff0c\u5176\u6e2c\u5f97\u7684\u6578\u503c\u70ba\u7d55\u5c0d\u55ae\u4f4d(mole)\uff0c\u975e\u4e00\u822c\u76f8\u5c0d\u55ae\u4f4d(RLU)<\/p>\n

    15<\/strong>\u5206\u9418\u5167\u5b8c\u6210<\/strong>ATP<\/strong>\u6d3b\u6027\u5206\u6790<\/strong><\/p>\n

    \u7d30\u83cc\u5916ATP\u4e4b\u79fb\u9664\u8207\u964d\u89e3\u904e\u7a0b\u5927\u7d0410\u5206\u9418\uff0c\u5176\u65bc\u6b65\u9a5f\u52a0\u5b8c\u5373\u5b8c\u6210\u3002\u5168\u90e8\u6d41\u7a0b\u5927\u7d0415\u5206\u5167\u5b8c\u6210<\/p>\n

    Microbial ATP KIT HS, 266-112<\/strong>\u7522\u54c1\u7279\u9ede\u6574\u7406<\/strong><\/p>\n

    Fast Assay: Results within minutes Highly Sensitive: 10-17 mol ATP (~ 5 bacteria) Reliable: Stable, Ready-to-use ATP Standard Cost-efficient: No Standard curve required Flexible: Choice of\u00a0\u03bc-plate & cuvette method<\/em><\/p>\n

    Microbial ATP KIT HS, 266-112<\/strong>\u7522\u54c1\u898f\u683c<\/strong><\/p>\n

    \u4e00\u7d44ATP reagent HS\u8a66\u5291\u53ef\u4ee5\u505a150~375\u6b21\u3002\u6bcf\u4e00\u5c0f\u7ba1\u53ef\u9032\u884c25\u500bTube luminometer\u53ca62.5\u500bmicroplate luminometer\u3002\u6574\u7d44kit\u5305\u62ec\u67096\u5c0f\u7ba1\uff0c\u6240\u4ee5\u53ef\u9032\u884c150\u500bTube luminometer\u53ca375\u500bmicroplate luminometer\u3002<\/p>\n

      \n
    1. ATP Reagent HS (6Vials). Lyophilized reagent contating luciferase and luciferin<\/li>\n
    2. Diluent B 10ml (6 vials). Buffer used to reconstitute ATP Reagent HS.<\/li>\n
    3. Extractant B\/S 10ml, (1 vial)<\/li>\n
    4. ATP standard 5ml (1 vial), contains 100nmol\/L ATP<\/li>\n
    5. ATP Eliminating Reagent (1 vial)<\/li>\n
    6. Cell lysing Reagent 10ml (1 vial). Used to reconstitute ATP Eliminating Reagnent.<\/li>\n<\/ol>\n

       <\/p>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      ATP Eliminating Reagent-ATP degrading enzyme Depending […]<\/p>\n","protected":false},"author":3,"featured_media":31776,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[912],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31775"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31775"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31775\/revisions"}],"predecessor-version":[{"id":31777,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31775\/revisions\/31777"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31776"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31775"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31775"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31775"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}