{"id":31658,"date":"2022-03-31T16:12:59","date_gmt":"2022-03-31T08:12:59","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31658"},"modified":"2022-07-19T11:42:55","modified_gmt":"2022-07-19T03:42:55","slug":"%e5%8d%b3%e7%94%a8%e5%9e%8bihc-%e8%a9%a6%e5%8a%91%e7%9b%92-detection-system-kit-mouserabbit-bioworld-%e8%b2%a8%e8%99%9fbd6001","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31658","title":{"rendered":"\u5373\u7528\u578bIHC \u8a66\u5291\u76d2 |Detection System kit-Mouse\/Rabbit -BioWorld \u8ca8\u865fBD6001"},"content":{"rendered":"

\"\u6a19\u7c6405\"<\/a>\u5373\u7528\u578bIHC Detection System kit \uff08Mouse\/Rabbit\uff09| BioWorld\u8ca8\u865fBD6001<\/a><\/span><\/p>\n

\"\u7522\u54c1\u4ecb\u7d39\"\u8d85\u9748\u654f\u6aa2\u6e2c\u7cfb\u7d71\u8a66\u5291\u76d2(Super Sensitive Detection System kit, BD6001)\u662f\u805a\u5408\u7269\u6a19\u8a18(Polymer detection methods)\u7684\u6700\u65b0\u6280\u8853\u3002\u805a\u5408\u7269\u6aa2\u6e2c\u65b9\u6cd5(Polymer detection methods)\u5df2\u88ab\u8b49\u660e\u53ef\u63d0\u4f9b\u66f4\u9ad8\u7684\u9748\u654f\u5ea6\u3002\u8207\u50b3\u7d71 IHC \u7cfb\u7d71\u76f8\u6bd4\uff0c\u9019\u7a2e\u5275\u65b0\u7684\u805a\u5408\u7269\u6280\u8853\u5177\u6709\u91cd\u5927\u512a\u52e2\u3002\u8d85\u9748\u654f\u6aa2\u6e2c\u7cfb\u7d71\u65bc\u4f7f\u7528\u5c0f\u9f20(mouse)\u548c\u5154(rabbit)\u4e00\u6297\u5177\u6709\u653e\u5927\u4fe1\u865f\u3002\u7531\u65bc\u8d85\u9748\u654f\u6aa2\u6e2c\u4e0d\u662f\u57fa\u65bc\u751f\u7269\u7d20\/\u6297\u751f\u7269\u7d20\u86cb\u767d\u7684\u7cfb\u7d71\uff0c\u56e0\u6b64\u6d88\u9664\u4e86\u7531\u65bc\u8207\u5167\u6e90\u6027\u751f\u7269\u7d20\u5206\u5b50\u975e\u7279\u7570\u6027\u7d50\u5408(nonspecific binding)\u800c\u5c0e\u81f4\u7684\u80cc\u666f\u566a\u8072(background noise)\uff0c\u8a72\u7cfb\u7d71\u6d88\u9664\u4e86\u7531\u65bc\u8207\u5167\u6e90\u6027\u751f\u7269\u7d20(endogenous biotin)\u975e\u7279\u7570\u6027\u7d50\u5408\u800c\u5c0e\u81f4\u7684\u80cc\u666f\u566a\u8072(background noise)\u3002\u8d85\u9748\u654f\u6aa2\u6e2c\u7cfb\u7d71\u70ba\u7528\u6236\u63d0\u4f9b\u4e86\u4e00\u7a2e\u5feb\u901f\u3001\u6613\u65bc\u4f7f\u7528\u4e14\u7528\u9014\u5ee3\u6cdb\u7684 IHC \u6aa2\u6e2c\u7cfb\u7d71\u3002<\/p>\n

\"bd6001\"<\/p>\n\n\n\n\n\n\n\n\n\n
Product Name<\/strong><\/td>\nSuper Sensitive TM IHC Detection System kit \uff08Mouse\/Rabbit\uff09<\/td>\n<\/tr>\n
Catalog No.<\/strong><\/td>\nBD6001<\/td>\n<\/tr>\n
Classification<\/strong><\/td>\nIHC Related Reagents<\/td>\n<\/tr>\n
Format<\/strong><\/td>\nKit<\/td>\n<\/tr>\n
Contents<\/strong><\/td>\nA:Hydrogen Peroxide Blocking ReagentB:Blocking ReagentC:Antibody AmplifierD:HRP PolymerE:DAB Substrate ReagentF:DAB Chromogen Reagent<\/td>\n<\/tr>\n
Storage & Shelf Life<\/strong><\/td>\nStore at 2-8\u00b0C. Each component is stable for up to 12 Months<\/td>\n<\/tr>\n
Research use<\/strong><\/td>\nFor research use only, not for use in diagnostic procedures.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u64cd\u4f5c\u624b\u518a\"<\/a><\/p>\n

Alternative Name :<\/strong>
\n1.\u00a0Within the validity period; 2.\u00a0Not intended to clinical diagnosis.<\/p>\n

Classification :<\/strong>
\nIHC Related Reagents<\/p>\n

Format :<\/strong>
\nKit<\/p>\n

Contents :<\/strong>
\nA:Hydrogen Peroxide Blocking Reagent<\/p>\n

B:Blocking Reagent<\/p>\n

C:Antibody Amplifier<\/p>\n

D:HRP Polymer<\/p>\n

E:DAB Substrate ReagentF:DAB Chromogen Reagent<\/p>\n

Storage & Shelf Life :<\/strong>
\nStore at 2-8\u00b0C. Each component is stable for up to 12 Months<\/p>\n

Procedure :<\/strong>
\n1.\u00a0Deparaffinize and rehydrate tissue section\uff1bPBS\/TBS wash for 2 min\u00d73\uff1b 2.\u00a0Incubate tissue in appropriate pretreatment or digestive enzyme if required for primary antibody; and PBS\/TBS wash for 2 min\u00d73; 3.\u00a0Incubate slide in Hydrogen Peroxide Blocking Reagent for 10 minutes, PBS\/TBS wash for 2 min\u00d73; 4.\u00a0Apply Blocking Reagent and incubate for 5 minutes, PBS\/TBS wash for 2 min\u00d73; (May be omitted if primary antibodies are diluted in buffers containing normal goat serum.); 5.\u00a0Apply primary antibody and incubate according to manufacturer\\’s recommended protocol\uff0cPBS\/TBS wash for 2 min\u00d73\uff1b 6.\u00a0Apply HRP Polymer and incubate for 10 min; (NOTE: HRP is light sensitive. Please avoid unnecessary light exposure.)\uff0cPBS\/TBS wash for 2 min\u00d73\uff1b 7.\u00a0Add 30 \u03bcl (1 drop) DAB Chromogen to 1 ml of DAB Substrate, mix by swirling and apply to tissue. Incubate for about 3-5 minutes\uff0cPBS\/TBS wash for 2 min\u00d73\uff1b 8.\u00a0Counterstain and coverslip using a permanent mounting media.<\/p>\n

Research use :<\/strong>
\nFor research use only, not for use in diagnostic procedures.<\/p>\n

\"\u54c1\u724c\u4ecb\u7d39\"<\/p>\n

Bioworld\u53f0\u7063\u4ee3\u7406\u5546-BRAND\/AGENCY\/\u4ee3\u7406\u54c1\u724c\/ Bioworld\u53f0\u7063\u4ee3\u7406\u5546<\/p>\n

Bioworld \u53f0\u7063\u4ee3\u7406\u5546<\/a><\/span><\/h2>\n

Bioworld\u64c1\u6709\u5c08\u696d\u7d93\u9a57\u8c50\u5bcc\u7684\u79d1\u5b78\u548c\u6280\u8853\u5c08\u696d\u4eba\u54e1\uff0c\u88fd\u9020\u548c\u92b7\u552e\u6297\u9ad4\u3002 \u6211\u5011\u76ee\u524d\u7684\u6297\u9ad4\u8986\u84cb\u7bc4\u570d\u5ee3\u6cdb\u7684\u4fe1\u865f\u8f49\u5c0e\u548c\u7d30\u80de\u6311\u9078\uff0c\u4f8b\u5982\uff1a<\/p>\n

1\uff09\u67d3\u8272\u8cea\u548c\u9170\u5316\u4fe1\u865f<\/p>\n

2\uff09MAPK\u4fe1\u865f<\/p>\n

3\uff09\u795e\u7d93\u5143SAPK \/ JNK\u4fe1\u865f<\/p>\n

4\uff09\u7d30\u80de\u51cb\u4ea1\u548cCaspase\u4fe1\u865f<\/p>\n

5\uff09AKT\u4fe1\u865f<\/p>\n

6\uff09\u8f49\u9304\u548c\u7ffb\u8b6f\u63a7\u5236<\/p>\n

7\uff09PKC\u4fe1\u865f<\/p>\n

8\uff09\u7d30\u80de\u9031\u671f\u548cDNA\u640d\u50b7\u4fe1\u865f<\/p>\n

9\uff09\u7d30\u80de\u9aa8\u67b6\u4fe1\u865f<\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u5373\u7528\u578bIHC Detection System kit \uff08Mouse\/Rabbit\uff09| BioWorld\u8ca8\u865fB […]<\/p>\n","protected":false},"author":3,"featured_media":31659,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[919],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31658"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31658"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31658\/revisions"}],"predecessor-version":[{"id":31672,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31658\/revisions\/31672"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31659"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31658"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31658"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31658"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}