{"id":31302,"date":"2022-03-22T18:33:32","date_gmt":"2022-03-22T10:33:32","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31302"},"modified":"2023-11-10T12:40:41","modified_gmt":"2023-11-10T04:40:41","slug":"chekine-%e7%be%a5%e5%9f%ba%e8%87%aa%e7%94%b1%e5%9f%ba%e6%8a%97%e6%b0%a7%e5%8c%96%e8%83%bd%e5%8a%9b-horac-%e8%9e%a2%e5%85%89%e6%aa%a2%e6%b8%ac%e8%a9%a6%e5%8a%91%e7%9b%92-%e8%b2%a8%e8%99%9f","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31302","title":{"rendered":"CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6297\u6c27\u5316\u80fd\u529b (HORAC) \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fKTB1502"},"content":{"rendered":"

CheKine\u2122 Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit<\/p>\n

CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6297\u6c27\u5316\u80fd\u529b (HORAC) \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2 | \u8ca8\u865fKTB1502<\/a><\/span><\/p>\n

CheKine\u2122 \u7fa5\u57fa\u81ea\u7531\u57fa\u6297\u6c27\u5316\u80fd\u529b (Hydroxyl Radical Antioxidant Capacity , HORAC) \u87a2\u5149\u6aa2\u6e2c\u8a66\u5291\u76d2\u70ba\u6aa2\u6e2c\u7fa5\u57fa\u81ea\u7531\u57fa\u6297\u6c27\u5316\u80fd\u529b (HORAC) \u63d0\u4f9b\u4e86\u65b9\u4fbf\u7684\u5de5\u5177\u3002 \u5176\u539f\u7406\u662f\u87a2\u5149\u7d20\u9209\u4f5c\u70ba\u87a2\u5149\u63a2\u91dd\uff0cFenton\u53cd\u61c9\u7522\u751f\u7fa5\u57fa\u81ea\u7531\u57fa\uff0c\u6839\u64da\u7fa5\u57fa\u81ea\u7531\u57fa\u5c0d\u87a2\u5149\u63a2\u91dd\u7684\u640d\u50b7\u7a0b\u5ea6\uff0c\u87a2\u5149\u5f37\u5ea6\u767c\u751f\u8b8a\u5316\uff0c\u87a2\u5149\u5f37\u5ea6\u7684\u8b8a\u5316\u53cd\u6620\u4e86\u81ea\u7531\u57fa\u640d\u50b7\u7684\u7a0b\u5ea6\u7fa5\u57fa\u81ea\u7531\u57fa\u5438\u5149\u5ea6(Hydroxyl Radical Absorbance Capacity , HORAC)\u662f\u4e00\u7a2e\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u751f\u7269\u5206\u5b50\u6297\u6c27\u5316\u80fd\u529b\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u53cd\u6620\u6297\u6c27\u5316\u5291\u8f49\u79fb\u7fa5\u57fa\u81ea\u7531\u57fa(Hydroxyl free radicals)\u7684\u80fd\u529b\u3002Hydroxyl Radical Absorbance Capacity (HORAC) is a method to detect the antioxidant Capacity of biological molecules in various samples, which can reflect the ability of antioxidants to transfer Hydroxyl free radicals.<\/p>\n

\u00a0(HORAC) \u87a2\u5149\u6aa2\u6e2c<\/a>\u00a0|\u00a0TAC\u7e3d\u6297\u6c27\u5316\u80fd\u529b\u6aa2\u6e2c\u00a0<\/a>\u00a0|\u00a0\u6c27\u81ea\u7531\u57fa\u6297\u6c27\u5316\u80fd\u529b (ORAC) \u87a2\u5149\u6aa2\u6e2c<\/a><\/span><\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n

\u6297\u6c27\u5316\u80fd\u529b\u5206\u6790\u6aa2\u6e2cAnalysis and detection of antioxidant capacity<\/p>\n

\u751f\u7269\u9ad4\u5167\u6709\u8a31\u591a\u6297\u6c27\u5316\u5291(antioxidants)\uff0c\u5982SOD\u3001CAT\u3001GSHPx\u3001G6PD\u3001GR\u3001\u7a40\u80f1\u7518\u80bd(glutathione)\u7b49\uff0c\u53ef\u4ee5\u6e05\u9664\u9ad4\u5167\u5404\u7a2e\u6d3b\u6027\u6c27\uff0c\u9632\u6b62\u6d3b\u6027\u6c27(reactive oxygen species)\u8a98\u5c0e\u7684\u6c27\u5316\u61c9\u6fc0(oxidative stress)\u7684\u7522\u751f\u3002\u4e00\u500b\u7cfb\u7d71\u4e2d\u5404\u7a2e\u6297\u6c27\u5316\u5927\u5206\u5b50\u3001\u6297\u6c27\u5316\u5c0f\u5206\u5b50\u548c\u9176\u7684\u7e3d\u6c34\u5e73\u53cd\u6620\u4e86\u7cfb\u7d71\u7684\u6297\u6c27\u5316\u80fd\u529b(antioxidant capacity)\u3002 \u96d6\u7136 ROS \u8a98\u5c0e\u7684\u6c27\u5316\u640d\u50b7\u7684\u5206\u6790\u88ab\u5ee3\u6cdb\u7528\u4f5c\u5206\u6790\u6307\u6a19\uff0c\u4f46\u6aa2\u6e2c\u9ad4\u5167\u7d30\u80de\u3001\u7d30\u80de\u548c\u63d0\u53d6\u7269\u7684\u6297\u6c27\u5316\u80fd\u529b\u6c34\u5e73\u4e5f\u5f88\u91cd\u8981\u3002 \u7d30\u80de\u5167\u5927\u5206\u5b50\u7684\u6297\u6c27\u5316\u80fd\u529b\u53ef\u4ee5\u901a\u904eORAC \uff08antioxidant capacity of oxygen group\uff0c\u6c27\u57fa\u7684\u6297\u6c27\u5316\u80fd\u529b\uff09\u3001HORAC\uff08antioxidant capacity of hydroxyl group, \u7fa5\u57fa\u7684\u6297\u6c27\u5316\u80fd\u529b\uff09\u548c\u7e3d\u6297\u6c27\u5316\u80fd\u529b\uff08total antioxidant capacity, TAC\uff09\u4f86\u8861\u91cf\u3002<\/p>\n

Abbkine \u7dca\u96a8 Cell Biolabs \u7684 ORAC \u548c HORAC \u751f\u5316\u5206\u6790\u3002\u6aa2\u6e2c\u65b9\u6cd5\u70ba\u904e\u6c27\u5316\u6c2b\u81ea\u7531\u57fa(hydrogen peroxide radical)\u901a\u904e\u6c2b\u539f\u5b50\u8f49\u79fb\u53cd\u61c9\u6c27\u5316\u87a2\u5149\u63a2\u91dd(oxidized fluorescence probe)\u3002\u5728\u7d44\u7e54\u7d30\u80de\u4e2d\uff0c\u904e\u6c27\u5316\u6c2b\u81ea\u7531\u57fa(hydrogen peroxide free radicals)\u662f\u7531\u5176\u4ed6\u5f15\u767c\u81ea\u7531\u57fa\u7684\u6c27\u5316(free radicals)\u7522\u751f\u7684\u3002\u5728\u6aa2\u6e2c\u904e\u7a0b\u4e2d\uff0c\u5f85\u6e2c\u6a23\u54c1\u4e2d\u7684\u6297\u6c27\u5316\u5291\u6291\u88fd\u904e\u6c27\u5316\u6c2b\u81ea\u7531\u57fa(hydrogen peroxide free radical)\u5c0d\u87a2\u5149\u63a2\u91dd\u7684\u6c27\u5316\uff0c\u76f4\u81f3\u5176\u6297\u6c27\u5316\u80fd\u529b(antioxidant capacity)\u55aa\u5931\u3002\u9019\u6a23\uff0c\u5269\u9918\u7684\u904e\u6c27\u5316\u6c2b\u81ea\u7531\u57fa\u6703\u7e7c\u7e8c\u6c27\u5316\u7834\u58de\u87a2\u5149\u63a2\u91dd\u7684\u87a2\u5149\u6548\u61c9(fluorescence effect)\uff0c\u76f4\u5230\u6297\u6c27\u5316\u5291\u7684\u6291\u5236\u6642\u9593\u548c\u81ea\u7531\u57fa\u640d\u50b7(free radical damage)\u7684\u6291\u5236\u9054\u5230\u5e73\u8861(balanced)\u3002\u56e0\u6b64\uff0c\u5f85\u6e2c\u6a23\u54c1\u7684\u6297\u6c27\u5316\u80fd\u529b(antioxidant capacity)\u8207\u87a2\u5149\u8870\u6e1b\u66f2\u7dda(fluorescence decay curve)\u6709\u95dc\u3002<\/p>\n

\u6c27\u81ea\u7531\u57fa\u5438\u6536\u80fd\u529b\uff08Oxygen Radical Absorbance Capacity , ORAC\uff09\u662f\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u751f\u7269\u5206\u5b50\u6297\u6c27\u5316\u80fd\u529b(antioxidant Capacity)\u7684\u7d93\u5178\u65b9\u6cd5\u3002 ORAC\u6cd5\u5177\u6709\u7279\u7570\u6027(specificity)\u597d\u3001\u9748\u654f\u5ea6\u9ad8(high sensitivity)\u3001\u6e2c\u5b9a\u7bc4\u570d\u5bec(wide determination range)\u3001\u6297\u6c27\u5316\u6d3b\u6027(antioxidant activity)\u7be9\u9078\u9ad8\u901a\u91cf\u7b49\u512a\u9ede\u3002 \u5b83\u5df2\u8d8a\u4f86\u8d8a\u591a\u5730\u61c9\u7528\u65bc\u5929\u7136\u7522\u7269\u6297\u6c27\u5316\u63d0\u53d6\u7269\u3002 \u98df\u54c1\u548c\u529f\u80fd\u6027\u98df\u54c1(functional food)\u4f01\u696d\u666e\u904d\u63a1\u7528ORAC\u4f5c\u70ba\u529f\u80fd\u6027\u98df\u54c1\u7684\u91cd\u8981\u8a55\u50f9\u6a19\u6e96\u3002<\/p>\n

\u7fa5\u57fa\u81ea\u7531\u57fa\u5438\u5149\u5ea6(Hydroxyl Radical Absorbance Capacity , HORAC)\u662f\u4e00\u7a2e\u6aa2\u6e2c\u5404\u7a2e\u6a23\u54c1\u4e2d\u751f\u7269\u5206\u5b50\u6297\u6c27\u5316\u80fd\u529b\u7684\u65b9\u6cd5\uff0c\u53ef\u4ee5\u53cd\u6620\u6297\u6c27\u5316\u5291\u8f49\u79fb\u7fa5\u57fa\u81ea\u7531\u57fa(Hydroxyl free radicals)\u7684\u80fd\u529b\u3002<\/p>\n\n\n\n\n
Product name<\/strong><\/td>\nCheKine\u2122 Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit<\/a><\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nCheKine\u2122 Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit provides a convenient tool for detection of Hydroxyl Radical Antioxidant Capacity (HORAC). The principle is that sodium fluorescein as fluorescent probe, Fenton reaction produces hydroxyl radical, according to the damage of hydroxyl radical to fluorescent probe, the fluorescence intensity changes, the change of fluorescence intensity reflects the degree of free radical damage.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u5be6\u9a57\u8a2d\u50992\"<\/a>\"\u00e7\u008e\u00bb\u00e7\u0089\u0087\u00e6\u008e\u0083\u00e6\u008f\u008f\"<\/a>\"ELISA<\/a><\/p>\n\n\n\n\n\n\n
Name<\/td>\nCat<\/td>\nSize<\/td>\n<\/tr>\n
CheKine\u2122 Total Antioxidant Capacity (TAC) Colorimetric Assay Kit<\/td>\nKTB1500<\/td>\n96T\/480T<\/td>\n<\/tr>\n
CheKine\u2122 Oxygen Radical Antioxidant Capacity (ORAC) Fluorometric Assay Kit<\/td>\nKTB1501<\/td>\n48T\/96T<\/td>\n<\/tr>\n
CheKine\u2122 Hydroxyl Radical Antioxidant Capacity (HORAC) Fluorometric Assay Kit<\/td>\nKTB1502<\/td>\n48T\/96T<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u5957\u7d44\u00a0<\/a>\u00a0|\u00a0\u00a0CCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong>\u00a0<\/a>\u00a0|\u00a0LDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a>\u00a0|\u00a0\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176<\/a>\u00a0|\u00a0\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2\u00a0<\/a><\/p>\n\n\n\n\n\n\n\n\n
\u8ca8\u865f<\/td>\n\u7522\u54c1\u540d\u7a31<\/td>\n\u80de\u5668\u67d3\u8272\u4f4d\u7f6e<\/td>\n\u87a2\u5149\u6ce2\u6bb5<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n\u7c92\u7dda\u9ad4(mitochondria)<\/td>\nEx \/ Em = 490\/523 nm<\/td>\n\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n\u5fae\u7ba1\u86cb\u767d(Tubulin)<\/td>\nEx\/Em = 500\/520 nm<\/td>\n\u6d3b\u7d30\u80de\u6216\u56fa\u5b9a\u4e4b\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n\u7d30\u80de\u819c<\/a>\u00a0(plasma membrane)<\/td>\nEx\/Em = 484\/501 nm<\/td>\n\u6d3b\u7d30\u80de\u3001\u56fa\u5b9a\u61f8\u6d6e\u3001\u8cbc\u9644\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n\u7d30\u80de\u6838 dsDNA<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\nLysosome<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n\u00a0\u67d3\u8272\u6d3b\u7d30\u80de\u548c\u56fa\u5b9a\u7d30\u80de<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u7522\u54c1\u8ca8\u865f<\/td>\n\u54c1\u9805<\/td>\n\u53cd\u61c9<\/td>\n\u6e2c\u91cf\u5100\u5668<\/td>\n<\/tr>\n
\u00a0\u8ca8\u865fKTA1001<\/a><\/td>\n\u6d3b\u7d30\u80de\u548c\u6b7b\u7d30\u80de\u96d9\u67d3\u8272\u8a66\u5291\u76d2<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\n

\u6d3b\u7d30\u80de\u7da0\u8272\u87a2\u5149\u67d3\u5291Ex\/Em = 488\/530 nm;<\/p>\n

\u6b7b\u7d30\u80de\u7d05\u8272\u87a2\u5149\u67d3\u6599(Ex\/Em = 535\/617)<\/td>\n<\/tr>\n

\n

\u8ca8\u865f\u00a0KTA2010<\/a><\/p>\n

 <\/td>\n

\n

TUNEL\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/p>\n

 <\/td>\n

50\u500b\u53cd\u61c9<\/td>\n\u87a2\u5149\u9176\u6a19\u5100\u3001\u87a2\u5149\u986f\u5fae\u93e1\u6216\u6d41\u5f0f\u7d30\u80de\u5100\u3002FITC\u901a\u9053\uff08Ex \/ Em = 490nm \/ 520 nm\uff09<\/td>\n<\/tr>\n
\n

\u8ca8\u865f\u00a0KTC011001<\/a><\/p>\n

 <\/td>\n

CCK-8\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/a><\/td>\n500\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4003<\/a><\/td>\nTraKine\u2122\u7c92\u7dda\u9ad4\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx \/ Em = 490\/523 nm\u589e\u6b96(proliferating)\u548c\u975e\u589e\u6b96\u7d30\u80de(non-proliferating cells)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4100<\/a><\/td>\nPro\u6d3b\u7d30\u80de\u5fae\u7ba1\u86cb\u767d\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\nEx\/Em = 500\/520 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4001<\/a><\/td>\nAbbkine\u00a0<\/a>\u7d30\u80de\u819c\u67d3\u8272\u00a0<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em = 484\/501 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n10MG\/100MG<\/td>\nEx\/Em: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTC4210<\/a><\/td>\n\u6d3b\u7d30\u80deLysosome\u67d3\u8272<\/a><\/td>\n50\u500b\u53cd\u61c9\/250\u500b\u53cd\u61c9<\/td>\ndeep red fluorescent dye , Ex\/Em:650\/665nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA3030<\/a><\/td>\n\u7d30\u80de\u8870\u8001\u6aa2\u6e2c\u8a66\u5291\u76d2\uff08\u03b2-\u534a\u4e73\u7cd6\u82f7\u9176\uff09<\/a><\/td>\n100\u500b\u53cd\u61c9<\/td>\n\u5149\u5b78\u986f\u5fae\u93e1<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1020<\/a><\/td>\nCCK-8<\/u><\/strong>\u7d30\u80de\u589e\u6b96\u548c\u7d30\u80de\u6bd2\u6027\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n1000\u500b\u53cd\u61c9\/10,000\u500b\u53cd\u61c9<\/td>\n\u5438\u6536\u5149450nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTA2030<\/a><\/td>\n\n

\u7d30\u80de\u589e\u6b96EdU Image\u8a66\u5291\u76d2<\/a><\/p>\n

(BrdU\u66ff\u4ee3\u6cd5)<\/td>\n

100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nAbFluor 488 azide (Ex\/Em = 501\/525 nm)<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1400<\/a><\/td>\n\u4e00\u6c27\u5316Dan\uff08NO\uff09\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (540 nm)<\/p>\n

\u6aa2\u6e2c\u7bc4\u570d\uff1a1-100 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/<\/td>\n<\/tr>\n

\u8ca8\u865fKTD3001<\/a><\/td>\n\u00a0BCA\u86cb\u767d\u5b9a\u91cf\u8a66\u5291\u76d2<\/a><\/td>\n\n

2000\u500b\u53cd\u61c9\/<\/p>\n

5000\u500b\u53cd\u61c9<\/td>\n

\n

OD 562 nm<\/p>\n

\u76f8\u5bb9\u6027 5 % SDS, 5 % Triton X-100, 5 % Tween-20, 60, 80<\/p>\n

50 to 1000 \u00b5g\/ml.<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1110<\/a><\/td>\n\u4e73\u9178\u812b\u6c2b\u9176\uff08LDH\uff09\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u03bbmax= 450 nm<\/p>\n

1- 20 U\/mL of LDH<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>PRP100153<\/a><\/td>\nIGF-I\u91cd\u7d44\u86cb\u767d<\/a><\/td>\n200UG\/1MG<\/td>\n\n

human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118)<\/p>\n

> 95 % as determined by SDS-PAGE<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTA2020<\/a><\/td>\n\u7d30\u80de\u9031\u671f\u67d3\u8272\u6aa2\u6e2c<\/a><\/td>\n100\u500b\u53cd\u61c9\/500\u500b\u53cd\u61c9<\/td>\nEx\/Em=535\/615 nm.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMC21400<\/a><\/td>\nPurKine\u2122\u5167\u6bd2\u7d20\u53bb\u9664\u9810\u88dd\u96e2\u5fc3\u67f1<\/a><\/td>\n\n

1ml\u00d75<\/p>\n

3mlx5<\/td>\n

More than 2,000,000 EU\/mL to eliminate >99% of endotoxins.<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1200<\/a><\/td>\nCheKine\u2122\u86cb\u767d\u7fb0\u57fa\u6bd4\u8272\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149370 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1080<\/a><\/td>\n\u8d85\u6c27\u9670\u96e2\u5b50\u6e05\u9664\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

Superoxide anion scavenging rate D% =\uff08\u2206\u2206ODControl – \u2206\u2206ODSample\uff09\u00f7 \u2206\u2206ODControl \u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1090<\/a><\/td>\n\u6e05\u9664\u6c2b\u6c27\u81ea\u7531\u57fa\u80fd\u529b\u6e2c\u5b9a\u8a66\u5291<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\u5438\u6536\u5149 536nm Hydroxyl radical scavenging rate D% =\uff08ASample – AControl\uff09\u00f7\uff08ABlank – AControl\uff09\u00d7100%<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB2220<\/a><\/td>\nTC\u7e3d\u81bd\u56fa\u9187\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

500nm Standard: 5 \u03bcmol\/mL<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1510<\/a><\/td>\nCheKine\u2122\u5c3f\u9178\uff08UA\uff09\u6bd4\u8272\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

48\u500b\u53cd\u61c9\/<\/p>\n

96\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u6536\u5149505 nm<\/p>\n

Standard: 5 \u03bcmol\/mL.<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1300<\/a><\/td>\nGlucose\u8461\u8404\u7cd6\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

192\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0\u5438\u5149\u503c630nm<\/p>\n

4- 300 mg\/dL)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1100<\/a><\/td>\nLactate\u00a0<\/u><\/strong><\/a> \u4e73\u9178\u6aa2\u6e2c\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

colorimetric (450 nm)<\/p>\n

0.03 mM-2 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1700<\/a><\/td>\n\u7d44\u7e54\u548c\u8840\u6db2\u9e7c\u6027\u78f7\u9178\u9176\uff08\u00a0Alkaline Phosphatase, AKP \/ ALP\uff09\u6bd4\u8272\u6e2c\u5b9a<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\u5438\u6536\u5149510 nm<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTB1500<\/a><\/td>\n\u7e3d\u6297\u6c27\u5316\u5291\u80fd\u529bTAC\u5206\u6790<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

593 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 0.15-3 mM<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTA0002<\/a><\/td>\nAnnexin V-AbFluor\u2122488\u7d30\u80de\u51cb\u4ea1\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n20\u500b\u53cd\u61c9\/50\u500b\u53cd\u61c9\/100\u500b\u53cd\u61c9<\/td>\nAnnexin V- AbFluor\u2122 488: Abs\/Em = 491\/517 nm; PI: Abs\/Em = 535\/617 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KET7013<\/a><\/td>\nEliKine\u2122 Rat IL-6 ELISA Kit<\/u><\/strong>\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 250pg\/mL-16000 pg\/mL, \u6700\u4f4e\u9748\u654f\u5ea6100 pg\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET0003<\/a><\/td>\n\u96cc\u4e8c\u9187\uff08Estradiol , E2\uff09\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d40 pg\/ml-1500 pg\/ml<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6032<\/a><\/td>\nTNF<\/u><\/em>–\u03b1<\/u><\/em>\u00a0Alpha\u578b\u816b\u7624\u58de\u6b7b\u56e0\u5b50 ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

7.8 pg\/ml-500 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET6033<\/a><\/td>\nVEGF\u8840\u7ba1\u5167\u76ae\u751f\u9577\u56e0\u5b50ELISA\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a031.25 pg\/mL-2000 pg\/mL<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KET7005<\/a><\/td>\n\u767d\u4ecb\u7d20-1\u03b2\uff08Interleukin-1\u03b2, IL-1\u03b2\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.<\/p>\n

\u5075\u6e2c\u7bc4\u570d:\u00a015.6 pg\/ml-1000 pg\/ml<\/u><\/strong><\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTE70003<\/a><\/td>\n\u5c0f\u9f20\u9ec3\u560c\u5464\u812b\u6c2b\u9176\/\u6c27\u5316\u9176\uff08XDH\uff09ELISA\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

96 X5\u76e4<\/td>\n

\n

450 nm<\/p>\n

\u5075\u6e2c\u7bc4\u570d: 22.5 ng\/L-360 ng\/L, \u6700\u4f4e\u9748\u654f\u5ea61.0 ng\/L\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f\u00a0A02051HRP<\/u><\/strong><\/a><\/td>\nHis-tag\u6a19\u7c64\u591a\u682a\u6297\u9ad4-HRP\u00a0<\/a><\/td>\n50ul\/200ul<\/td>\nWB (1:5000).<\/td>\n<\/tr>\n
\u8ca8\u865fA02170MGB<\/a><\/td>\nAnti-V5 Tag Mouse Monoclonal Antibody (11D5), \u78c1\u73e0<\/a><\/td>\n100ul\/400ul\/2ml<\/td>\nGKPIPNPLLGLDST epitope<\/td>\n<\/tr>\n
\u8ca8\u865fKTA4001<\/a><\/td>\nJC-1<\/u><\/strong>\u7dda\u7c92\u9ad4\u819c\u96fb\u4f4d\u6e2c\u5b9a\u8a66\u5291\u76d2<\/u><\/strong><\/a><\/td>\n\n

100\u500b\u53cd\u61c9\/<\/p>\n

500\u500b\u53cd\u61c9<\/td>\n

\n

\uff08\u805a\u7d50\u7684JC-1\uff0cEx \/ Em = 585\/590 nm\uff09\u767c\u51fa\u7da0\u8272\u87a2\u5149\uff08JC-1\u55ae\u9ad4\uff0cEx \/ Em = 510\/527 nm\uff09<\/p>\n

JC-1 Stain<\/p>\n

CCCP (10 mM)\u00a0 Assay Buffer (5\u00d7)<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1600<\/a><\/td>\n\u7a40\u80f1\u7518\u80bd(Glutathione)\u6aa2\u6e2c<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

412 nm<\/p>\n

2-200 \u00b5g\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>BMD00063<\/a><\/td>\nDAPI Staining Solution<\/a><\/td>\n1MG\/10MG\/100MG<\/td>\n\u03bbEx\/\u03bbEm: 358\/461 nm (with DNA)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTP3001<\/a><\/td>\n\u7d30\u80de\u6838\/\u7d30\u80de\u8cea\u5206\u96e2\u8a66\u5291\u76d2<\/a><\/td>\n\n

50\u500b\u53cd\u61c9\/<\/p>\n

200\u500b\u53cd\u61c9<\/td>\n

Cytoplasmic Solution A (CES A) Cytoplasmic Solution B (CES B) \u2022 Nuclear Extraction Solution (NES) DTT (500X) Protease Inhibitor (100X)<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>BMD00064-50UG<\/a><\/td>\nCalcein AM\u9223\u9ec3\u7da0\u7d20\u6d3b\u7d30\u80de\u87a2\u5149\u67d3\u5291<\/a><\/td>\n50ug\/1mg\/10mg<\/td>\n\n

\u03bbEX\/\u03bbEm: 494\/517 nm (pH 8) (calcein)<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>\u00a0KTB1660<\/a><\/td>\n\u786b\u6c27\u9084\u86cb\u767d\u904e\u6c27\u5316\u7269\u9176\uff08TPX\uff09\u6aa2\u6e2c\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n240nm<\/td>\n<\/tr>\n
\u8ca8\u865fKTB1020<\/a><\/td>\nNAD\/NADH \u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD565 nm<\/p>\n

96 \u5b54\u677f\u6e2c\u5b9a\u4e2d\u7684\u6aa2\u6e2c\u9650\u503c\u70ba 0.78 \u03bcM \u7dda\u6027\u6700\u9ad8 50 \u03bcM NAD+\/NADH\u3002<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1070<\/a><\/td>\nCheKine\u2122\u9ec3\u560c\u5464\u6c27\u5316\u9176\u6aa2\u6e2c<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 450 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d15.6 -500 mU\/mL<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1040<\/a><\/td>\nCatalase\u904e\u6c27\u5316\u6c2b\u9176\u6d3b\u6027\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

OD 540 nm<\/p>\n

2-75 \u00b5M<\/td>\n<\/tr>\n

\u8ca8\u865f<\/u><\/strong>KTB1060<\/a><\/td>\n\u904e\u6c27\u5316\u7269\u9176\uff08Peroxidases\uff09\u5206\u6790\u8a66\u5291\u76d2<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 664 nm<\/p>\n

\u7dda\u6027\u7bc4\u570d\u70ba15.6-1000 \u00b5U\/mL<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1050<\/a><\/td>\n\u8102\u8cea\u904e\u6c27\u5316\u6aa2\u6e2c\u8a66\u5291\u76d2<\/a>(MDA)<\/td>\n96\u500b\u53cd\u61c9<\/td>\n\n

OD 532 nm<\/p>\n

1-100 \u00b5M\/L<\/td>\n<\/tr>\n

\u8ca8\u865fKTB1030<\/a><\/td>\n\u8d85\u6c27\u5316\u7269\u6b67\u5316\u9176\uff08SOD\uff09\u6d3b\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2\u00a0<\/a><\/td>\n96\u500b\u53cd\u61c9<\/td>\n200-3.13 U\/ml<\/td>\n<\/tr>\n
\u8ca8\u865f<\/u><\/strong>KTA1030<\/a><\/td>\nLDH\u7d30\u80de\u6bd2\u6027\u6e2c\u5b9a\u8a66\u5291\u76d2<\/a><\/td>\n\n

96\u500b\u53cd\u61c9\/<\/p>\n

480\u500b\u53cd\u61c9<\/td>\n

\n

\u00a0OD 565 nm<\/p>\n

10,00-100,0000 cells per well<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

CheKine\u2122 Hydroxyl Radical Antioxidant Capacity (HORAC) […]<\/p>\n","protected":false},"author":3,"featured_media":28512,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[940],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31302"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31302"}],"version-history":[{"count":5,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31302\/revisions"}],"predecessor-version":[{"id":46758,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31302\/revisions\/46758"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28512"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31302"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31302"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31302"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}