{"id":31265,"date":"2022-03-21T15:21:35","date_gmt":"2022-03-21T07:21:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31265"},"modified":"2023-11-13T16:54:49","modified_gmt":"2023-11-13T08:54:49","slug":"%e4%ba%ba%e7%b8%bd%e8%86%bd%e6%b1%81%e9%85%b8-tba%e8%a9%a6%e5%8a%91%e7%9b%92-human-total-bile-acid-tba-elisa-kit%e8%b2%a8%e8%99%9fkte63032","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31265","title":{"rendered":"\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u8a66\u5291\u76d2 | Human Total bile acid (TBA) ELISA Kit\u8ca8\u865fKTE63032"},"content":{"rendered":"

\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u8a66\u5291\u76d2 | <\/strong>Human Total bile acid (TBA) ELISA Kit\u8ca8\u865f<\/strong>KTE63032<\/a><\/span><\/p>\n

<\/p>\n

\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u8a66\u5291\u76d2\u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u3002\u5c0d\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u7279\u7570\u7684\u6297\u9ad4\u5df2\u9810\u5148\u5305\u88ab\u5728\u5fae\u5b54\u677f\u4e0a\u3002\u5c07\u6a19\u6e96\u54c1\u548c\u6a23\u54c1\u5438\u53d6\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u5b58\u5728\u7684\u4efb\u4f55\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u90fd\u88ab\u56fa\u5b9a\u7684\u6297\u9ad4\u7d50\u5408\u3002\u9664\u53bb\u6240\u6709\u672a\u7d50\u5408\u7684\u7269\u8cea\u5f8c\uff0c\u5c07HRP-\u7db4\u5408\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u6aa2\u6e2c\u6297\u9ad4\u6dfb\u52a0\u5230\u5b54\u4e2d\u3002\u6d17\u6ecc\u4ee5\u9664\u53bb\u4efb\u4f55\u672a\u7d50\u5408\u7684HRP\u8a66\u5291\u5f8c\uff0c\u5c07\u8272\u539f\u6eb6\u6db2\u6dfb\u52a0\u5230\u5b54\u4e2d\uff0c\u4e26\u4e14\u6839\u64da\u5728\u521d\u59cb\u6b65\u9a5f\u4e2d\u7d50\u5408\u7684\u4eba\u7e3d\u81bd\u6c41\u9178 (TBA)\u7684\u91cf\u6210\u6bd4\u4f8b\u5730\u986f\u8272\u3002\u505c\u6b62\u986f\u8272\u4e26\u6e2c\u91cf\u984f\u8272\u7684\u5f37\u5ea6\u3002\u5075\u6e2c\u7bc4\u570d: 1 mmol\/L-16 mmol\/L, \u6700\u4f4e\u9748\u654f\u5ea60.1 mmol\/L\u3002<\/u><\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n

<\/p>\n

\"\u00e5\u008e\u009f\u00e5\u00bb\u00a0\u00e6\u0093\u008d\u00e4\u00bd\u009c\u00e4\u00b8\u008b\u00e8\u00bc\u0089\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nHuman Total bile acid (TBA) ELISA Kit<\/a><\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA<\/td>\n<\/tr>\n
Applications notes<\/strong><\/td>\nHuman Total bile acid (TBA) ELISA Kit employs a two-site sandwich ELISA to quantitate TBA in samples. An antibody specific for TBA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TBA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugate Human TBA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of TBA bound in the initial step. The color development is stopped and the intensity of the color is measured.<\/td>\n<\/tr>\n
Detection method<\/strong><\/td>\nColorimetric<\/td>\n<\/tr>\n
SampleType<\/strong><\/td>\nCell culture supernatants, Other biological fluids, Plasma, Serum<\/td>\n<\/tr>\n
Assay type<\/strong><\/td>\nSandwich ELISA<\/td>\n<\/tr>\n
Calibration range<\/strong><\/td>\n1 mmol\/L-16 mmol\/L<\/td>\n<\/tr>\n
Limit of detection<\/strong><\/td>\n0.1 mmol\/L<\/td>\n<\/tr>\n
Usage notes<\/strong><\/td>\n\u2022 Do not mix components from different kit lots or use reagents beyond the kit expiration date.
\n\u2022 Allow all reagents to warm to room temperature for at least 30 minutes before opening.
\n\u2022 Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
\n\u2022 Unused wells must be kept desiccated at 4 \u00b0C in the sealed bag provided.
\n\u2022 Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
\n\u2022 It is recommended that all samples and standards be assayed in duplicate or triplicate.<\/td>\n<\/tr>\n
Storage instructions<\/strong><\/td>\nThe unopened kit should be stored at 2 – 8\u00b0C. After opening, please store refer to protocols.<\/td>\n<\/tr>\n
Shipping<\/strong><\/td>\nGel pack with blue ice.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u9175\u7d20\u9023\u7d50\u514d\u75ab\u5438\u9644\u6e2c\u5b9a\u6cd5\uff08ELISA\uff09\u662f\u4e00\u7a2e\u5e38\u7528\u65bc\u6e2c\u91cf\u751f\u7269\u6a23\u672c\u4e2d\u7684\u6297\u9ad4(antibodies)\u3001\u6297\u539f( antigens)\u3001\u86cb\u767d\u8cea(proteins)\u548c\u7cd6\u86cb\u767d( glycoproteins )\u7684\u514d\u75ab\u5b78\u6e2c\u5b9a\u3002\u4e00\u4e9b\u4f8b\u5b50\u5305\u62ec\uff1aHIV \u611f\u67d3( HIV infection)\u7684\u8a3a\u65b7\u3001\u598a\u5a20\u8a66\u9a57(pregnancy tests)\u4ee5\u53ca\u7d30\u80de\u4e0a\u6e05\u6db2\u6216\u8840\u6e05\u4e2d\u7d30\u80de\u56e0\u5b50(cytokines)\u6216\u53ef\u6eb6\u6027\u53d7\u9ad4(soluble receptors)\u7684\u6e2c\u91cf\u3002 ELISA\u539f\u7406\u53ef\u5206\u70ba\u593e\u5fc3ELISA(sandwich ELISA)\u3001\u7af6\u722d\u6027ELISA(competitive ELISA )\u548c\u9593\u63a5ELISA(competitive ELISA )\u3002<\/p>\n

<\/p>\n

\"\u7db2\u7ad9\u898f\u683c\u5c0f\u5716_\u5de5\u4f5c\u5340\u57df<\/a><\/strong><\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u529f\u80fd<\/strong><\/p>\n

\u7d30\u80de\u56e0\u5b50(Cytokine)\u5177\u6709\u591a\u7a2e\u751f\u7269\u5b78\u529f\u80fd\u3002\u5b83\u662f\u6025\u6027\u671f\u53cd\u61c9(acute phase response)\u7684\u6709\u6548\u8a98\u5c0e\u5291\u3002\u5728B\u7d30\u80de(B-cells)\u6700\u7d42\u5206\u5316\u70baIg\u5206\u6ccc\u7d30\u80de(Ig-secreting cells)\u7684\u6700\u7d42\u5206\u5316\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\uff0c\u53c3\u8207\u6dcb\u5df4\u7d30\u80de(lymphocyte)\u548c\u55ae\u6838\u7d30\u80de(monocyte)\u7684\u5206\u5316\u3002\u5b83\u8a98\u5c0e\u9aa8\u9ad3\u7624(myeloma)\u548c\u6f3f\u7d30\u80de\u7624(plasmacytoma growth)\u751f\u9577\uff0c\u4e26\u8a98\u5c0e\u795e\u7d93\u7d30\u80de\u5206\u5316(differentiation)\u3002\u4f5c\u7528\u65bcB\u7d30\u80de(B-cells)\uff0cT\u7d30\u80de(T-cells)\uff0c\u809d\u7d30\u80de(hepatocytes)\uff0c\u9020\u8840\u7956\u7d30\u80de(hematopoeitic progenitor cells)\u548cCNS\u4e2d\u6a1e\u795e\u7d93\u7cfb\u7d71\u7d30\u80de\u3002\u4e5f\u53ef\u4ee5\u4f5c\u70ba\u808c\u8089\u56e0\u5b50(myokine)\u3002\u5b83\u5728\u808c\u8089\u6536\u7e2e\u5f8c\u6392\u5165\u8840\u6db2\uff0c\u4e26\u8d77\u5230\u589e\u52a0\u8102\u80aa\u5206\u89e3\u548c\u6539\u5584\u80f0\u5cf6\u7d20\u62b5\u6297(insulin resistance)\u7684\u4f5c\u7528\u3002<\/p>\n

\u708e\u6027\u7d30\u80de\u56e0\u5b50<\/strong><\/span><\/p>\n

\u708e\u75c7\u7531\u591a\u7a2e\u53ef\u6eb6\u6027\u56e0\u5b50\u4ecb\u5c0e\uff0c\u5305\u62ec\u4e00\u7d44\u7a31\u70ba\u7d30\u80de\u56e0\u5b50\u7684\u5206\u6ccc\u591a\u80bd\u3002\u708e\u6027\u7d30\u80de\u56e0\u5b50\u53ef\u5206\u70ba\u5169\u985e\uff1a\u53c3\u8207\u6025\u6027\u708e\u75c7\u7684\u7d30\u80de\u56e0\u5b50\u548c\u8ca0\u8cac\u4efb\u7684\u7d30\u80de\u56e0\u5b50\u3002\u708e\u75c7\u662f\u7d44\u7e54\u5c0d\u640d\u50b7\u7684\u53cd\u61c9\uff0c\u5176\u7279\u5fb5\u662f\u5728\u6025\u6027\u671f\u4ee5\u589e\u52a0\u7684\u8840\u6d41\u91cf\u548c\u8840\u7ba1\u901a\u900f\u6027\u4ee5\u53ca\u9ad4\u6db2\uff0c\u767d\u7d30\u80de\u548c\u708e\u6027\u4ecb\u8cea\uff08\u4f8b\u5982\u7d30\u80de\u56e0\u5b50\uff09\u7684\u7a4d\u7d2f\u70ba\u7279\u5fb5\u3002\u5e7e\u7a2e\u7d30\u80de\u56e0\u5b50\u5728\u4ecb\u5c0e\u6025\u6027\u708e\u75c7\u53cd\u61c9\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\uff0c\u5373IL-1\uff0cTNF-\u03b1\uff0cIL-6\uff0cIL-11\uff0cIL-8\u548c\u5176\u4ed6\u8da8\u5316\u56e0\u5b50\uff0cG-CSF\u548cGM-CSF\u3002<\/p>\n

\u5df2\u77e5\u53ef\u4ecb\u5c0e\u6162\u6027\u708e\u75c7\u904e\u7a0b\u7684\u7d30\u80de\u56e0\u5b50\u53ef\u5206\u70ba\u53c3\u8207\u9ad4\u6db2\u708e\u75c7\u7684\u7d30\u80de\u56e0\u5b50\uff0c\u4f8b\u5982IL-3\uff0cIL-4\uff0cIL-5\uff0cIL-6\uff0cIL-7\uff0cIL-9\uff0cIL-10\uff0cIL- 13\uff0c\u8f49\u5316\u751f\u9577\u56e0\u5b50b\uff08TGF-b\uff09\uff0c\u4ee5\u53ca\u90a3\u4e9b\u6709\u52a9\u65bc\u7d30\u80de\u708e\u75c7\u7684\u56e0\u5b50\uff0c\u4f8b\u5982IL-1\uff0cIL-2\uff0cIL-3\uff0cIL-4\uff0cIL-7\uff0cIL-9\uff0cIL-10\uff0c IL-12\uff0c\u5e72\u64fe\u7d20\uff08IFN\uff09\uff0cIFN-g\u8a98\u5c0e\u56e0\u5b50\uff08IGIF\uff09\uff0cTGF-b\u548cTNF-a\u548c-b\u3002\u708e\u75c7\u7570\u5e38\u662f\u4e00\u5927\u985e\u75be\u75c5\uff0c\u662f\u8a31\u591a\u4eba\u985e\u75be\u75c5\u7684\u57fa\u790e\u3002\u7d30\u80de\u56e0\u5b50\u662f\u5bbf\u4e3b\u5c0d\u611f\u67d3\uff0c\u514d\u75ab\u53cd\u61c9\uff0c\u708e\u75c7\u548c\u5275\u50b7\u53cd\u61c9\u7684\u8abf\u7bc0\u5291\u3002\u4e00\u4e9b\u7d30\u80de\u56e0\u5b50\u8d77\u4f7f\u75be\u75c5\u60e1\u5316\u7684\u4f5c\u7528\uff08\u4fc3\u708e\u7d30\u80de\u56e0\u5b50\uff09\uff0c\u800c\u53e6\u4e00\u4e9b\u7d30\u80de\u56e0\u5b50\u8d77\u6e1b\u8f15\u708e\u75c7\u548c\u4fc3\u9032\u7652\u5408\u7684\u4f5c\u7528\uff08\u6297\u708e\u7d30\u80de\u56e0\u5b50\uff09\u3002<\/p>\n

\u767d\u4ecb\u7d20-1\uff08Interleukin-1, IL-1\uff09\u662f\u6709\u53f2\u4ee5\u4f86\u6700\u65e9\u7684\u7d30\u80de\u56e0\u5b50(cytokines)\u4e4b\u4e00\u3002\u5b83\u7684\u6700\u521d\u767c\u73fe\u662f\u53ef\u80fd\u8a98\u767c\u767c\u71d2(fever)\uff0c\u63a7\u5236\u6dcb\u5df4\u7d30\u80de(lymphocytes)\uff0c\u589e\u52a0\u9aa8\u9ad3\u7d30\u80de\u6578\u91cf(bone marrow cells)\u4e26\u5f15\u8d77\u9aa8\u95dc\u7bc0\u9000\u5316\u7684\u56e0\u7d20\u3002\u6b64\u6642\uff0cIL-1\u4ee5\u5176\u4ed6\u5e7e\u7a2e\u540d\u7a31\u70ba\u4eba\u6240\u77e5\uff0c\u5305\u62ec\u5167\u6e90\u6027\u71b1\u539f(endogenous pyrogen)\uff0c\u6dcb\u5df4\u7d30\u80de\u6d3b\u5316\u56e0\u5b50(lymphocyte activating factor)\uff0c\u9020\u8840\u7d20-1(haemopoetin-1)\u548c\u55ae\u6838\u7d30\u80de\u56e0\u5b50(mononuclear cell factor)\u7b49\u3002\u5927\u7d04\u57281984\u5e74\u81f31985\u5e74\u9593\uff0c\u79d1\u5b78\u5bb6\u5011\u8b49\u5be6IL-1\u5be6\u969b\u4e0a\u7531\u5169\u7a2e\u4e0d\u540c\u7684\u86cb\u767d\u8cea\u7d44\u6210\uff0c\u73fe\u5728\u7a31\u70baIL-1\u03b1\u548cIL-1\u03b2\uff0c\u5b83\u5011\u5c6c\u65bc\u7a31\u70ba\u767d\u7d30\u80de\u4ecb\u7d201\u8d85\u5bb6\u65cf\u7684\u7d30\u80de\u56e0\u5b50\u5bb6\u65cf\u3002 IL-1\u03b2\u7531\u5de8\u566c\u7d30\u80de(macrophages)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\u548c\u6a39\u7a81\u72c0\u7d30\u80de(dendritic cells)\u7522\u751f\u3002<\/p>\n

IFN-\u03b3\uff08IFN-gamma, \u5e72\u64fe\u7d20-\u03b3\uff09\u662f\u4fc3\u708e(proinflammatory)\u7d30\u80de\u56e0\u5b50(cytokine)\u7684\u539f\u578b(prototype)\uff0c\u7531\u591a\u7a2e\u514d\u75ab\u7d30\u80de(immune cells)\u5728\u708e\u6027(inflammatory)\u689d\u4ef6\u4e0b\u7522\u751f\uff0c\u7279\u5225\u662f\u7531T\u7d30\u80de(T cells)\u548cNK\u7d30\u80de(NK cells)\u7522\u751f\u3002\u5b83\u901a\u904e\u4fc3\u9032Th1\u7d30\u80de\u7684\u767c\u80b2(development)\u548c\u6fc0\u6d3b(activation)\uff0c\u55ae\u6838\u7d30\u80de(monocytes)\u548c\u5de8\u566c\u7d30\u80de(macrophage)\u7684\u5316\u5b78\u5f15\u8a98(chemoattraction)\u548c\u6fc0\u6d3b(activation)\uff0c\u6297\u539f\u5448\u905e\u5206\u5b50(antigen presentation molecules)\u7684\u4e0a\u8abf\u4ee5\u53caB\u7d30\u80de\u4e2d\u7684\u514d\u75ab\u7403\u86cb\u767d\u985e\u5225\u8f49\u63db(immunoglobulin class switching)\uff0c\u5728\u5bbf\u4e3b\u9632\u79a6\u4e2d\u8d77\u95dc\u9375\u4f5c\u7528\u3002\u5b83\u9084\u5177\u6709\u6297\u75c5\u6bd2(antiviral)\uff0c\u6297\u589e\u6b96(antiproliferative)\u548c\u51cb\u4ea1\u4f5c\u7528(apoptotic)\u3002\u53e6\u5916\uff0cIFN-\u03b3\u901a\u904e\u4fc3\u9032\u8abf\u7bc0\u6027T\u7d30\u80de\u7684\u767c\u80b2\u4e26\u6291\u5236Th17\u7d30\u80de\u5206\u5316\u800c\u5145\u7576\u6297\u708e\u4ecb\u8cea\u3002 IFN-\u03b3\u4e8c\u805a\u9ad4\u901a\u904e\u5169\u500bIFN-\u03b3R1\u548c\u5169\u500bIFN-\u03b3R2\u4e9e\u57fa\u7684\u53d7\u9ad4\u8907\u5408\u7269\u767c\u51fa\u4fe1\u865f\u3002<\/p>\n

\u767d\u7d30\u80de\u4ecb\u7d2017 (Interleukin 17, IL17)<\/p>\n

IL-17\u57fa\u56e0\u7de8\u78bc( IL-17 gene)\u7684\u86cb\u767d\u8cea\u662f\u7531\u6d3b\u5316\u7684T\u7d30\u80de(activated T cells)\u6240\u7522\u751f\u7684\u4fc3\u767c\u708e\u7d30\u80de\u6fc0\u7d20\u3002\u6b64\u7d30\u80de\u6fc0\u7d20\u8abf\u7bc0 NF-\u03baB \u548c\u7d72\u88c2\u539f\u6d3b\u5316\u86cb\u767d\u6fc0\u9176(mitogen-activated protein kinases)\u7684\u6d3b\u6027\u3002\u9019\u7a2e\u7d30\u80de\u6fc0\u7d20(cytokine )\u53ef\u4ee5\u523a\u6fc0 IL6 \u548c\u74b0\u6c27\u5408\u9176-2 (cyclooxygenase-2, PTGS2\/COX-2) \u7684\u8868\u9054\uff0c\u4e26\u589e\u5f37\u4e00\u6c27\u5316\u6c2e (nitric oxide, NO) \u7684\u7522\u751f\u3002\u9ad8\u6c34\u5e73\u7684\u9019\u7a2e\u7d30\u80de\u6fc0\u7d20\u8207\u591a\u7a2e\u6162\u6027\u767c\u708e\u6027\u75be\u75c5\u6709\u95dc\uff0c\u5305\u62ec\u985e\u98a8\u6fd5\u6027\u95dc\u7bc0\u708e(rheumatoid arthritis)\u3001\u725b\u76ae\u766c(psoriasis )\u548c\u591a\u767c\u6027\u786c\u5316\u75c7(multiple sclerosis)\u3002<\/p>\n

\u767d\u8840\u7403\u4ecb\u7d20-8\uff08Interleukin-8, IL8)<\/p>\n

IL-8\u57fa\u56e0\u7de8\u78bc(IL-8 gene)\u7684\u86cb\u767d\u8cea\u662fCXC\u8da8\u5316\u56e0\u5b50\u5bb6\u65cf(CXC chemokine family)\u7684\u6210\u54e1\u3002\u9019\u7a2e\u8da8\u5316\u56e0\u5b50(chemokine )\u662f\u767c\u708e\u53cd\u61c9(inflammatory response)\u7684\u4e3b\u8981\u4ecb\u8cea\u4e4b\u4e00\u3002\u9019\u7a2e\u8da8\u5316\u56e0\u5b50\u7531\u591a\u7a2e\u7d30\u80de\u985e\u578b\u5206\u6ccc\u3002\u5b83\u5177\u6709\u5316\u5b78\u5f15\u8a98\u5291( chemoattractant)\u7684\u4f5c\u7528\uff0c\u4e5f\u662f\u4e00\u7a2e\u6709\u6548\u7684\u8840\u7ba1\u751f\u6210\u56e0\u5b50(angiogenic factor)\u3002 IL-8\u57fa\u56e0\u88ab\u8a8d\u70ba\u5728\u7d30\u652f\u6c23\u7ba1\u708e\uff08\u4e00\u7a2e\u7531\u75c5\u6bd2\u611f\u67d3\u5f15\u8d77\u7684\u5e38\u898b\u547c\u5438\u9053\u75be\u75c5\uff09\u7684\u767c\u75c5\u6a5f\u5236(pathogenesis )\u4e2d\u767c\u63ee\u4f5c\u7528\u3002 IL-8\u57fa\u56e0(IL-8 gene )\u548cCXC\u8da8\u5316\u56e0\u5b50\u57fa\u56e0\u5bb6\u65cf(CXC chemokine gene family)\u7684\u5176\u4ed610\u500b\u6210\u54e1\u5728\u5b9a\u4f4d\u65bc\u67d3\u8272\u9ad44q( chromosome 4q)\u7684\u5340\u57df\u4e2d\u5f62\u6210\u8da8\u5316\u56e0\u5b50\u57fa\u56e0\u7c07(chemokine gene cluster )\u3002<\/p>\n

<\/p>\n