{"id":31239,"date":"2022-03-18T15:29:11","date_gmt":"2022-03-18T07:29:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31239"},"modified":"2022-07-19T11:49:45","modified_gmt":"2022-07-19T03:49:45","slug":"human-sglt2-sodiumglucose-cotransporter-2-elisa-kit-hufi03401%e8%b2%a8%e8%99%9fhufi03401","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31239","title":{"rendered":"Human SGLT2 (Sodium\/glucose cotransporter 2) ELISA Kit (HUFI03401)\u8ca8\u865fHUFI03401"},"content":{"rendered":"

Human SGLT2 (Sodium\/glucose cotransporter 2) ELISA Kit (HUFI03401)\u8ca8\u865f<\/strong>HUFI03401<\/a><\/span><\/p>\n

SGLT2 (Sodium\/glucose cotransporter 2) encodes a sodium-dependent glucose transport protein, which is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in SGLT2 (Sodium\/glucose cotransporter 2) are associated with renal glucosuria and aminoaciduria. The Assay Genie Human SGLT2 (Sodium\/glucose cotransporter 2) ELISA is a highly sensitive assay for the quantitative measurement of SGLT2 (Sodium\/glucose cotransporter 2) in serum, blood, plasma, cell culture supernatant and tissue samples.<\/p>\n

\"Human<\/p>\n

SGLT2\uff08Sodium\/glucose cotransporter 2, \u9209\/\u8461\u8404\u7cd6\u5354\u540c\u8f49\u904b\u86cb\u767d 2\uff09\u7de8\u78bc\u9209\u4f9d\u8cf4\u6027\u8461\u8404\u7cd6\u8f49\u904b\u86cb\u767d(sodium-dependent glucose transport protein)\uff0c\u5b83\u662f\u53c3\u8207\u814e\u81df\u8461\u8404\u7cd6\u91cd\u5438\u6536(glucose reabsorption) \u7684\u4e3b\u8981\u5354\u540c\u8f49\u904b\u86cb\u767d(cotransporter)\u3002 SGLT2\uff08\u9209\/\u8461\u8404\u7cd6\u5354\u540c\u8f49\u904b\u86cb\u767d 2\uff09\u7684\u7a81\u8b8a(Mutations)\u8207\u814e\u6027\u7cd6\u5c3f(renal glucosuria)\u548c\u6c28\u57fa\u9178\u5c3f(aminoaciduria)\u6709\u95dc\u3002 Assay Genie Human SGLT2\uff08\u9209(Sodium )\/\u8461\u8404\u7cd6\u5354\u540c\u8f49\u904b\u86cb\u767d 2(glucose cotransporter 2)\uff09ELISA \u662f\u4e00\u7a2e\u9ad8\u5ea6\u9748\u654f\u7684\u6e2c\u5b9a\u65b9\u6cd5\uff0c\u7528\u65bc\u5b9a\u91cf\u6e2c\u91cf\u8840\u6e05\u3001\u8840\u6db2\u3001\u8840\u6f3f\u3001\u7d30\u80de\u57f9\u990a\u4e0a\u6e05\u6db2\u548c\u7d44\u7e54\u6a23\u54c1\u4e2d\u7684 SGLT2\uff08\u9209\/\u8461\u8404\u7cd6\u5354\u540c\u8f49\u904b\u86cb\u767d 2\uff09<\/p>\n

Human SGLT2 (Sodium\/glucose cotransporter 2) ELISA<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"SGLT2\uff08Sodium\/glucose cotransporter 2\uff09ELISA\u8a66\u5291\u76d2\u63a1\u7528\u5169\u9ede\u593e\u5fc3ELISA(Sandwich ELISA)\u5b9a\u91cf\u6a23\u54c1\u4e2d\u7684SGLT2\u3002\u5075\u6e2c\u7bc4\u570d: 15.625-1000pg\/ml<\/u><\/strong>, \u6700\u4f4e\u9748\u654f\u5ea69.375pg\/ml<\/u><\/strong>\u3002<\/u><\/strong>\u53ef\u61c9\u7528\u65bc\u4ee5\u4e0b\u6a23\u54c1\u4f86\u6e90: Cell culture supernatants, Other biological fluids, Plasma, Serum<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
ELISA Microplate (Dismountable)<\/td>\n8\u00d712 strips<\/td>\n4\u00b0C for 6 months<\/td>\n<\/tr>\n
Lyophilized Standard<\/td>\n2<\/td>\n4\u00b0C\/-20\u00b0C<\/td>\n<\/tr>\n
Sample\/Standard Dilution Buffer<\/td>\n20ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Biotin-labeled Antibody(Concentrated)<\/td>\n120ul<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
Antibody Dilution Buffer<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
HRP-Streptavidin Conjugate(SABC)<\/td>\n120ul<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
SABC Dilution Buffer<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n10ml<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
Stop Solution<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Wash Buffer(25X)<\/td>\n30ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Plate Sealer<\/td>\n5<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

KU:<\/strong><\/p>\n

HUFI03401<\/p>\n

PRODUCT TYPE:<\/strong><\/p>\n

ELISA Kit<\/p>\n

SIZE:<\/strong><\/p>\n

96 Assays<\/p>\n

UNIPROT:<\/strong><\/p>\n

P31639<\/p>\n

SENSITIVITY:<\/strong><\/p>\n

9.375pg\/ml<\/p>\n

RANGE:<\/strong><\/p>\n

15.625-1000pg\/ml<\/p>\n

ELISA TYPE:<\/strong><\/p>\n

Sandwich ELISA, Double Antibody<\/p>\n

SYNONYMS:<\/strong><\/p>\n

Sodium\/glucose cotransporter 2, Na(+)\/glucose cotransporter 2, Low affinity sodium-glucose cotransporter, Solute carrier family 5 member 2, SLC5A2, SGLT2<\/p>\n

REACTIVITY:<\/strong><\/p>\n

Human<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name:<\/strong><\/td>\nHuman SGLT2 (Sodium\/glucose cotransporter 2) ELISA Kit<\/td>\n<\/tr>\n
Product Code:<\/strong><\/td>\nHUFI03401<\/td>\n<\/tr>\n
Size:<\/strong><\/td>\n96 Assays<\/td>\n<\/tr>\n
Alias:<\/strong><\/td>\nSodium\/glucose cotransporter 2 ELISA Kit, Na(+)\/glucose cotransporter 2 ELISA Kit, Low affinity sodium-glucose cotransporter ELISA Kit, Solute carrier family 5 member 2 ELISA Kit, SLC5A2 ELISA Kit, SGLT2 ELISA Kit<\/td>\n<\/tr>\n
Detection method:<\/strong><\/td>\nSandwich ELISA, Double Antibody<\/td>\n<\/tr>\n
Application:<\/strong><\/td>\nThis immunoassay kit allows for the in vitro quantitative determination of Human SGLT2 (Sodium\/glucose cotransporter 2) concentrations in serum plasma and other biological fluids.<\/td>\n<\/tr>\n
Sensitivity:<\/strong><\/td>\n< 9.375pg\/ml<\/td>\n<\/tr>\n
Range:<\/strong><\/td>\n15.625-1000pg\/ml<\/td>\n<\/tr>\n
Storage:<\/strong><\/td>\n4\u00b0C for 6 months<\/td>\n<\/tr>\n
Note:<\/strong><\/td>\nFor Research Use Only<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Component<\/strong><\/td>\nQuantity<\/strong><\/td>\nStorage<\/strong><\/td>\n<\/tr>\n
ELISA Microplate (Dismountable)<\/td>\n8\u00d712 strips<\/td>\n4\u00b0C for 6 months<\/td>\n<\/tr>\n
Lyophilized Standard<\/td>\n2<\/td>\n4\u00b0C\/-20\u00b0C<\/td>\n<\/tr>\n
Sample\/Standard Dilution Buffer<\/td>\n20ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Biotin-labeled Antibody(Concentrated)<\/td>\n120ul<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
Antibody Dilution Buffer<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
HRP-Streptavidin Conjugate(SABC)<\/td>\n120ul<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
SABC Dilution Buffer<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
TMB Substrate<\/td>\n10ml<\/td>\n4\u00b0C (Protect from light)<\/td>\n<\/tr>\n
Stop Solution<\/td>\n10ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Wash Buffer(25X)<\/td>\n30ml<\/td>\n4\u00b0C<\/td>\n<\/tr>\n
Plate Sealer<\/td>\n5<\/td>\n–<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n
Sample Type<\/strong><\/td>\nProtocol<\/strong><\/td>\n<\/tr>\n
Serum<\/strong><\/td>\n\n

If using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80\u00b0C. Avoid multiple freeze-thaw cycles.<\/p>\n

If serum separator tubes are not being used, allow samples to clot overnight at 2-8\u00b0C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80\u00b0C. Avoid multiple freeze-thaw cycles.<\/td>\n<\/tr>\n

Plasma<\/strong><\/td>\nCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4\u00b0C for 15 mins at 1000 \u00d7 g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80\u00b0C. Avoid multiple freeze-thaw cycles.\u00a0Note:<\/strong>\u00a0Over haemolysed samples are not suitable for use with this kit.<\/td>\n<\/tr>\n
Urine & Cerebrospinal Fluid<\/strong><\/td>\nCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.<\/td>\n<\/tr>\n
Cell culture supernatant<\/strong><\/td>\nCollect the cell culture media by pipette, followed by centrifugation at 4\u00b0C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.<\/td>\n<\/tr>\n
Cell lysates<\/strong><\/td>\nSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at \u2264 -20 \u00b0C.<\/td>\n<\/tr>\n
Tissue homogenates<\/strong><\/td>\nThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at \u2264 -20\u00b0C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20\u00b0C or -80\u00b0C.<\/td>\n<\/tr>\n
Tissue lysates<\/strong><\/td>\nRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at \u2264 -20 \u00b0C.<\/td>\n<\/tr>\n
Breast Milk<\/strong><\/td>\nCollect milk samples and centrifuge at 10,000 x g for 60 min at 4\u00b0C. Aliquot the supernatant and assay. For long term use, store samples at -80\u00b0C. Minimize freeze\/thaw cycles.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

Human SGLT2 (Sodium\/glucose cotransporter 2) ELISA Kit […]<\/p>\n","protected":false},"author":3,"featured_media":31162,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[947],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31239"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31239"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31239\/revisions"}],"predecessor-version":[{"id":31243,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31239\/revisions\/31243"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31162"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31239"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31239"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31239"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}