{"id":31210,"date":"2022-03-17T16:02:46","date_gmt":"2022-03-17T08:02:46","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31210"},"modified":"2022-07-19T11:50:11","modified_gmt":"2022-07-19T03:50:11","slug":"acute-kidney-injury-aki-5-plex-immunoassay-panel-assay-genie-multiplex-elisa%e8%b2%a8%e8%99%9fhuampm054","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31210","title":{"rendered":"Acute Kidney Injury (AKI) 5-Plex Immunoassay Panel | Assay Genie Multiplex ELISA\u8ca8\u865fHUAMPM054"},"content":{"rendered":"

GeniePlex\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853 (Multiplex ELISA Array, Assay Genie Multiplex ELISA)<\/p>\n

Acute Kidney Injury (AKI) 5-Plex Immunoassay Panel, \u8ca8\u865fHUAMPM054<\/a><\/span><\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"GeniePlex \u591a\u91cd\u6aa2\u6e2c\u662f\u4e00\u7a2e\u57fa\u65bc\u5fae\u73e0\u7684\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853\uff0c\u5e7e\u4e4e\u53ef\u4ee5\u5728\u4efb\u4f55\u6d41\u5f0f\u7d30\u80de\u5100\u4e0a\u540c\u6642\u5b9a\u91cf\u6aa2\u6e2c\u55ae\u500b 15ul \u6a23\u672c\u4e2d\u7684\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u3002\u53ef\u4ee5\u4e00\u500bWell\u540c\u6642\u5075\u6e2c24 \u7a2e\u4e0d\u540c\u7684 ELISA\uff01\u76ee\u524d\u7522\u54c1\u7a2e\u985e\u5f88\u591a\uff0c\u5305\u542b:apoptosis, inflammation, angiogenesis, immune response \u548cmigration, \u53ef\u7531\u4eba\u985e\u3001\u5c0f\u9f20\u3001\u5927\u9f20\u3001\u975e\u4eba\u985e\u9748\u9577\u985e\u52d5\u7269\u548c\u72ac\u79d1\u52d5\u7269\u7684 400 \u591a\u7a2e\u5206\u6790\u7269\u4e2d\u9032\u884c\u9078\u64c7\u3002\u5927\u591a\u6578\u6aa2\u6e2c\u7684\u9748\u654f\u5ea6\u4f4e\u65bc 10.0 pg\/ml\u3002\u6e2c\u5b9a\u6642\u9593\u7d04 4\u500b\u5c0f\u6642\u3002\u6e2c\u5b9a\u5167 CV\uff1a<10%\uff0c\u6e2c\u5b9a\u9593 <20% ( Intra-Assay CV: <10%, Inter-Assay < 20%) \u3002\u5df2\u7d93\u958b\u767c\u4e86\u5e7e\u7a2e\u57fa\u65bc\u6297\u9ad4\u7684\u6e2c\u5b9a\u5e73\u53f0(antibody-based assay platforms)\u4f5c\u70ba ELISA \u7684\u66ff\u4ee3\u54c1\uff0c\u7528\u65bc\u540c\u6642\u5b9a\u91cf\u5206\u6790\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u5206\u6790\u7269\uff0c\u4e5f\u7a31\u70ba\u591a\u91cd\u6e2c\u5b9a(multiplex assays)\u3002\u6700\u5e38\u898b\u7684\u591a\u91cd\u514d\u75ab(multiplex immunoassays)\u5206\u6790\u5e73\u53f0\u4f7f\u7528\u57fa\u65bc\u6d41\u52d5\u7684\u6280\u8853(flow-based technology)\u548c\u6297\u9ad4\u5305\u88ab\u7684\u73e0\u5b50(antibody-coated beads)\u3002\u73e0\u5b50\u53ef\u7528\u65bc\u540c\u6642\u6e2c\u91cf\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u591a\u7a2e\u5206\u6790\u7269(multiple analytes)\u3002\u5728\u591a\u91cd\u5206\u6790(multiplex assay)\u4e2d\uff0c\u7279\u5b9a\u5206\u6790\u7269\u7684\u7279\u7570\u6027\u6297\u9ad4\u8207\u5206\u6790\u4e2d\u7684\u4e00\u7d44\u73e0\u5b50\u76f8\u9023\u3002\u7136\u5f8c\u6dfb\u52a0\u53e6\u4e00\u7a2e\u6297\u9ad4\uff0c\u8a72\u6297\u9ad4\u9644\u6709\u87a2\u5149\u5831\u544a\u57fa\u56e0\u3002\u6bcf\u7d44\u73e0\u5b50\u90fd\u6709\u4e00\u7a2e\u76f8\u95dc\u7684\u984f\u8272\uff0c\u5f9e\u800c\u53ef\u4ee5\u540c\u6642\u6aa2\u6e2c\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u591a\u7a2e\u5206\u6790\u7269\u3002\u5206\u6790\u7269\u7684\u6aa2\u6e2c\u53ef\u4ee5\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u8b80\u53d6\uff0c\u56e0\u70ba\u73e0\u5b50\u53ef\u4ee5\u901a\u904e\u5404\u7a2e\u87a2\u5149\u7279\u5fb5\u6aa2\u6e2c\u3002\u53ef\u4ee5\u6839\u64da\u6aa2\u6e2c\u5230\u7684\u4e0d\u540c\u73e0\u5b50\u984f\u8272\u7684\u6578\u91cf\u4f86\u91cf\u5316\u5206\u6790\u7269\u7684\u91cf\u3002Assay Genie Multiplex ELISA \u8a66\u5291\u76d2\u5141\u8a31\u7814\u7a76\u4eba\u54e1\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u8853\u4f7f\u7528\u4f4e\u81f3 15\u03bcL \u7684\u91cf\u5c0d\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u9032\u884c\u591a\u91cd\u5206\u6790\uff01<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Product Name:<\/strong><\/td>\nAcute Kidney Injury (AKI) 5-Plex Immunoassay Panel<\/td>\n<\/tr>\n
Product Code:<\/strong><\/td>\nHUAMPM054<\/td>\n<\/tr>\n
Reactivity:<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Analytes:<\/strong><\/td>\nIP-10, TIM-1, OPN, NGAL, Cystatin C<\/td>\n<\/tr>\n
Sensitivity (LODs):<\/strong><\/td>\nCystatin C: < 20 pg\/mL, IP-10: < 3 pg\/mL, NGAL: < 50 pg\/mL, OPN: < 5 pg\/mL, TIM-1: < 5 pg\/mL<\/td>\n<\/tr>\n
LLOQs:<\/strong><\/td>\nCystatin C: < 40 pg\/mL, IP-10: < 10 pg\/mL, NGAL: < 100 pg\/mL, OPN: < 10 pg\/mL, TIM-1: < 10 pg\/mL<\/td>\n<\/tr>\n
Sample types:<\/strong><\/td>\nCell culture supernatant, serum, plasma, bodily fluid and tissue\/cell lysate<\/td>\n<\/tr>\n
Assay Type:<\/strong><\/td>\nMultiplex<\/td>\n<\/tr>\n
Detection Method:<\/strong><\/td>\nFlow Cytometry<\/td>\n<\/tr>\n
Assay Time:<\/strong><\/td>\n2 hours<\/td>\n<\/tr>\n
1x Ab-conjugated beads (premixed):<\/strong><\/td>\nS4P3 – anti-IP-10, S4P6 – anti-human TIM-1, S4P7 – anti-human OPN, S5P2 – anti-human NGAL, S5P9 – anti-human Cystatin C<\/td>\n<\/tr>\n
2x Biotin-detection Ab (premixed):<\/strong><\/td>\nBiotin anti-human IP-10, Bioin – anti-human TIM-1, Biotin – anti-human OPN, Biotin – anti-human NGAL, Biotin – anti-human Cystatin C<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/tr>\n
Buffers:<\/strong><\/td>\n2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent.<\/td>\n<\/tr>\n
Lyophilized Standard Mix:<\/strong><\/td>\nLyophilized Standard Mix-Human T Helper Cytokine 4-Plex Panel 2. One vial containing lyophilized recombinant IFN-Gamma, IL-4, IL-10, TNF-Alpha.<\/td>\n<\/tr>\n
LLOQs:<\/strong><\/td>\nCystatin C: < 40 pg\/mL,IP-10: < 10 pg\/mL,NGAL: < 100 pg\/mL,OPN: < 10 pg\/mL,TIM-1: < 10 pg\/mL<\/td>\n<\/tr>\n
ULOQs:Cystatin C: > 20,000 pg\/mL, IP-10: > 2,000 pg\/mL, NGAL: > 50,000 pg\/mL, \u00a0,OPN: > 5,000 pg\/mL, TIM-1: > 5,000 pg\/mL<\/strong><\/td>\n<\/td>\n<\/tr>\n
Standard dose recovery:<\/strong><\/td>\n70-130%<\/td>\n<\/tr>\n
Intra-assay CV:<\/strong><\/td>\nIntra-assay CV: < 10%<\/td>\n<\/tr>\n
Inter-assay CV:<\/strong><\/td>\nInter-assay CV: < 20%<\/td>\n<\/tr>\n
<\/td>\nNegligible<\/td>\n<\/tr>\n
Sample volume:<\/strong><\/td>\n15 \u00b5L\/test<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

 <\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"\u591a\u91cdELISA\u539f\u7406<\/p>\n

Assay Genie Multiplex ELISA \u5229\u7528\u6309\u5927\u5c0f\u548c\u4e0d\u540c\u87a2\u5149\u5f37\u5ea6\u6c34\u5e73\u5340\u5206\u7684\u591a\u500b\u73e0\u7fa4\u3002\u591a\u91cd\u5c3a\u5bf8\u7684\u73e0\u5b50\u548c\u6bcf\u500b\u73e0\u5b50\u5c3a\u5bf8\u7684\u591a\u500b\u87a2\u5149\u5f37\u5ea6\u6c34\u5e73\uff0c\u591a\u91cd\u6280\u8853\u53ef\u4ee5\u5728\u55ae\u500b\u53cd\u61c9\u4e2d\u540c\u6642\u6e2c\u91cf\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u3002\u591a\u91cd ELISA \u53ea\u9700\u8981 15\u03bcL \u7684\u6a23\u54c1\u3002\u53cd\u61c9\u4e2d\u7684\u73e0\u5b50\u6578\u91cf\u7531\u914d\u5099\u55ae\u500b 488nm \u6fc0\u5149\u5668\u6216\u96d9 488nm \u548c 633\/640nm \u6fc0\u5149\u5668\u7684\u6d41\u5f0f\u7d30\u80de\u5100\u78ba\u5b9a\u3002\u73e0\u5206\u985e\u67d3\u6599\u7684\u6700\u5927\u767c\u5c04\u6ce2\u9577\u70ba 700 nm\u3002<\/p>\n

\u57fa\u65bc\u73e0\u5b50\u7684\u514d\u75ab\u6e2c\u5b9a\u985e\u4f3c\u65bc\u593e\u5fc3 ELISA \u7684\u539f\u7406\uff0c\u6bcf\u500b\u73e0\u5b50\u7fa4\u8207\u6355\u7372\u6a23\u54c1\u4e2d\u611f\u8208\u8da3\u7684\u86cb\u767d\u8cea\uff08\u4f8b\u5982\u7d30\u80de\u56e0\u5b50\uff09\u7684\u7279\u7570\u6027\u6355\u7372\u6297\u9ad4\u5076\u806f\u3002\u901a\u904e\u91dd\u5c0d\u86cb\u767d\u8cea\u4e8c\u7d1a\u8868\u4f4d\u7684\u751f\u7269\u7d20\u5316\u6297\u9ad4\u6aa2\u6e2c\u6355\u7372\u7684\u5206\u6790\u7269\u7684\u91cf\uff0c\u7136\u5f8c\u9032\u884c\u93c8\u9ef4\u89aa\u548c\u7d20-R-\u85fb\u7d05\u86cb\u767d\u8655\u7406\u3002\u73e0\u5b50\u4e0a\u7684 R-\u85fb\u7d05\u86cb\u767d\u7684\u87a2\u5149\u5f37\u5ea6\u5728\u6d41\u5f0f\u7d30\u80de\u5100\u4e0a\u9032\u884c\u91cf\u5316\u3002\u901a\u904e\u5c07\u87a2\u5149\u4fe1\u865f\u8207\u7531\u5df2\u77e5\u6fc3\u5ea6\u7684\u5206\u6790\u7269\u7684\u9023\u7e8c\u7a00\u91cb\u7522\u751f\u7684\u6a19\u6e96\u66f2\u7dda\u7684\u87a2\u5149\u4fe1\u865f\u9032\u884c\u6bd4\u8f03\uff0c\u53ef\u4ee5\u7372\u5f97\u6a23\u54c1\u4e2d\u611f\u8208\u8da3\u7684\u86cb\u767d\u8cea\u7684\u6fc3\u5ea6\u3002\u6e2c\u5b9a\u7a0b\u5e8f\u5305\u62ec 60 \u5206\u9418\u7684\u6297\u539f\u548c\u6355\u7372\u6297\u9ad4\u5076\u806f\u73e0\u5b75\u80b2\u6b65\u9a5f\u300130 \u5206\u9418\u7684\u751f\u7269\u7d20\u5316\u6aa2\u6e2c\u5b75\u80b2\u6b65\u9a5f\u548c 20 \u5206\u9418\u7684\u93c8\u9ef4\u89aa\u548c\u7d20 PE \u5b75\u80b2\u6b65\u9a5f\u3002<\/p>\n

 <\/p>\n

\u6a23\u54c1\u5f62\u5f0f:<\/p>\n

Cell Culture Supernatants\u3001Plasma\u3001Serum\u3001Tissue Lysates\u3001 Cell Lysates<\/p>\n

 <\/p>\n

Features & Benefits<\/p>\n

    \n
  • Analyze: Multiplex 2-24 analytes in a single sample!<\/li>\n
  • Fast: 2 hour protocol!<\/li>\n
  • Sensitive: Measure as low as <10 pg\/ml of each analyte<\/li>\n
  • Dynamic: Lower limit < 20 pg\/mL | Upper limit > 5,000 pg\/mL<\/li>\n
  • High Precision Intra-assay CV: < 10% | Inter-assay CV: < 20%<\/li>\n
  • Low Volumes: Use as little as 15\u03bcl of sample<\/li>\n
  • Validated: All assays fully tested for cross-reactivity in our lab<\/li>\n<\/ul>\n

     <\/p>\n

    Multiplex vs. Luminex?<\/h2>\n

    The Assay Genie Multiplex ELISA gives excellent performance versus Luminex technology. The Human TH1\/TH2\/TH17 7-Plex ELISA and a Luminex multiplex assay were used to assay cell culture supernatant samples from human PBMCs treated with various reagents.<\/p>\n

     <\/p>\n

     <\/p>\n

    Over 400 Analytes to measure!<\/h2>\n
      \n
    • Comprehensive Choice of Targets<\/li>\n
    • Up to 400 assays & custom formats available for human, mouse, rat, porcine, canine and primates<\/li>\n
    • Wide Range of Sample Types<\/li>\n
    • Cell culture supernatants, saliva, plasma, cell\/tissue lysates, serum, BALF, pleural and peritoneal fluids & more<\/li>\n
    • Multiple Formats: 32-well and 96-well sizes available<\/li>\n
    • Key Premixed Panels<\/li>\n
    • We have designed key panels to measure Cytokines, Chemokines, Inflammation, Th1\/Th2 & Th1\/Th2\/Th17<\/li>\n<\/ul>\n

       <\/p>\n","protected":false},"excerpt":{"rendered":"

      GeniePlex\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853 (Multiplex ELISA Array, Assay Genie M […]<\/p>\n","protected":false},"author":3,"featured_media":31205,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[949],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31210"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31210"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31210\/revisions"}],"predecessor-version":[{"id":31212,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31210\/revisions\/31212"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31205"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31210"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31210"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31210"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}