{"id":31204,"date":"2022-03-17T15:55:35","date_gmt":"2022-03-17T07:55:35","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=31204"},"modified":"2022-07-19T11:50:21","modified_gmt":"2022-07-19T03:50:21","slug":"%e3%80%90genieplex%e5%a4%9a%e9%87%8d%e5%85%8d%e7%96%ab%e6%aa%a2%e6%b8%ac%e6%8a%80%e8%a1%93%e3%80%91-multiplex-elisa-array-assay-genie-multiplex-elisa-genieplex-multiplex-assays","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=31204","title":{"rendered":"GeniePlex\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853 |  Multiplex ELISA Array, Assay Genie Multiplex ELISA , GeniePlex Multiplex Assays"},"content":{"rendered":"<p>GeniePlex\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853 (Multiplex ELISA Array, Assay Genie Multiplex ELISA)<\/p>\n<p><video controls=\"controls\" width=\"300\" height=\"150\"><source src=\"&lt;iframe width=&quot;416&quot; height=&quot;311&quot; src=&quot;https:\/\/www.youtube.com\/embed\/dsQLADkK7sk&quot; title=&quot;YouTube video player&quot; frameborder=&quot;0&quot; allow=&quot;accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture&quot; allowfullscreen&gt;&lt;\/iframe&gt;\" \/><\/video><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E7%94%A2%E5%93%81%E4%BB%8B%E7%B4%B9-300x111.jpg\" alt=\"\u00e7\u0094\u00a2\u00e5\u0093\u0081\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"102\" height=\"37\" \/>GeniePlex \u591a\u91cd\u6aa2\u6e2c\u662f\u4e00\u7a2e\u57fa\u65bc\u5fae\u73e0\u7684\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853\uff0c\u5e7e\u4e4e\u53ef\u4ee5\u5728\u4efb\u4f55\u6d41\u5f0f\u7d30\u80de\u5100\u4e0a\u540c\u6642\u5b9a\u91cf\u6aa2\u6e2c\u55ae\u500b 15ul \u6a23\u672c\u4e2d\u7684\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u3002\u53ef\u4ee5\u4e00\u500bWell\u540c\u6642\u5075\u6e2c24 \u7a2e\u4e0d\u540c\u7684 ELISA\uff01\u76ee\u524d\u7522\u54c1\u7a2e\u985e\u5f88\u591a\uff0c\u5305\u542b:apoptosis, inflammation, angiogenesis, immune response \u548cmigration, \u53ef\u7531\u4eba\u985e\u3001\u5c0f\u9f20\u3001\u5927\u9f20\u3001\u975e\u4eba\u985e\u9748\u9577\u985e\u52d5\u7269\u548c\u72ac\u79d1\u52d5\u7269\u7684 400 \u591a\u7a2e\u5206\u6790\u7269\u4e2d\u9032\u884c\u9078\u64c7\u3002\u5927\u591a\u6578\u6aa2\u6e2c\u7684\u9748\u654f\u5ea6\u4f4e\u65bc 10.0 pg\/ml\u3002\u6e2c\u5b9a\u6642\u9593\u7d04 4\u500b\u5c0f\u6642\u3002\u6e2c\u5b9a\u5167 CV\uff1a&lt;10%\uff0c\u6e2c\u5b9a\u9593 &lt;20% ( Intra-Assay CV: &lt;10%, Inter-Assay &lt; 20%) \u3002<\/p>\n<p>\u5df2\u7d93\u958b\u767c\u4e86\u5e7e\u7a2e\u57fa\u65bc\u6297\u9ad4\u7684\u6e2c\u5b9a\u5e73\u53f0(antibody-based assay platforms)\u4f5c\u70ba ELISA \u7684\u66ff\u4ee3\u54c1\uff0c\u7528\u65bc\u540c\u6642\u5b9a\u91cf\u5206\u6790\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u5206\u6790\u7269\uff0c\u4e5f\u7a31\u70ba\u591a\u91cd\u6e2c\u5b9a(multiplex assays)\u3002\u6700\u5e38\u898b\u7684\u591a\u91cd\u514d\u75ab(multiplex immunoassays)\u5206\u6790\u5e73\u53f0\u4f7f\u7528\u57fa\u65bc\u6d41\u52d5\u7684\u6280\u8853(flow-based technology)\u548c\u6297\u9ad4\u5305\u88ab\u7684\u73e0\u5b50(antibody-coated beads)\u3002\u73e0\u5b50\u53ef\u7528\u65bc\u540c\u6642\u6e2c\u91cf\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u591a\u7a2e\u5206\u6790\u7269(multiple analytes)\u3002<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/i.shgcdn.com\/44af9eb6-7778-4bfe-826d-46f0ee87f801\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"\" width=\"443\" height=\"368\" \/><\/p>\n<p>\u5728\u591a\u91cd\u5206\u6790(multiplex assay)\u4e2d\uff0c\u7279\u5b9a\u5206\u6790\u7269\u7684\u7279\u7570\u6027\u6297\u9ad4\u8207\u5206\u6790\u4e2d\u7684\u4e00\u7d44\u73e0\u5b50\u76f8\u9023\u3002\u7136\u5f8c\u6dfb\u52a0\u53e6\u4e00\u7a2e\u6297\u9ad4\uff0c\u8a72\u6297\u9ad4\u9644\u6709\u87a2\u5149\u5831\u544a\u57fa\u56e0\u3002\u6bcf\u7d44\u73e0\u5b50\u90fd\u6709\u4e00\u7a2e\u76f8\u95dc\u7684\u984f\u8272\uff0c\u5f9e\u800c\u53ef\u4ee5\u540c\u6642\u6aa2\u6e2c\u55ae\u500b\u6a23\u54c1\u4e2d\u7684\u591a\u7a2e\u5206\u6790\u7269\u3002\u5206\u6790\u7269\u7684\u6aa2\u6e2c\u53ef\u4ee5\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u5100\u8b80\u53d6\uff0c\u56e0\u70ba\u73e0\u5b50\u53ef\u4ee5\u901a\u904e\u5404\u7a2e\u87a2\u5149\u7279\u5fb5\u6aa2\u6e2c\u3002\u53ef\u4ee5\u6839\u64da\u6aa2\u6e2c\u5230\u7684\u4e0d\u540c\u73e0\u5b50\u984f\u8272\u7684\u6578\u91cf\u4f86\u91cf\u5316\u5206\u6790\u7269\u7684\u91cf\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>Assay Genie Multiplex ELISA \u8a66\u5291\u76d2\u5141\u8a31\u7814\u7a76\u4eba\u54e1\u901a\u904e\u6d41\u5f0f\u7d30\u80de\u8853\u4f7f\u7528\u4f4e\u81f3 15\u03bcL \u7684\u91cf\u5c0d\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u9032\u884c\u591a\u91cd\u5206\u6790\uff01<\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/i.shgcdn.com\/efa24636-87d9-40b3-9d71-5e3b5e49833d\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"GeniePlex Multiplex Assay\" width=\"1016\" height=\"487\" \/><\/p>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"http:\/\/biopioneer.com.tw\/wp-content\/uploads\/2021\/08\/%E5%8E%9F%E7%90%86%E4%BB%8B%E7%B4%B9-300x114.png\" alt=\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" width=\"93\" height=\"35\" \/><\/p>\n<p>\u591a\u91cdELISA\u539f\u7406-Assay Genie Multiplex ELISA \u5229\u7528\u6309\u5927\u5c0f\u548c\u4e0d\u540c\u87a2\u5149\u5f37\u5ea6\u6c34\u5e73\u5340\u5206\u7684\u591a\u500b\u73e0\u7fa4\u3002\u591a\u91cd\u5c3a\u5bf8\u7684\u73e0\u5b50\u548c\u6bcf\u500b\u73e0\u5b50\u5c3a\u5bf8\u7684\u591a\u500b\u87a2\u5149\u5f37\u5ea6\u6c34\u5e73\uff0c\u591a\u91cd\u6280\u8853\u53ef\u4ee5\u5728\u55ae\u500b\u53cd\u61c9\u4e2d\u540c\u6642\u6e2c\u91cf\u591a\u9054 24 \u7a2e\u5206\u6790\u7269\u3002\u591a\u91cd ELISA \u53ea\u9700\u8981 15\u03bcL \u7684\u6a23\u54c1\u3002\u53cd\u61c9\u4e2d\u7684\u73e0\u5b50\u6578\u91cf\u7531\u914d\u5099\u55ae\u500b 488nm \u6fc0\u5149\u5668\u6216\u96d9 488nm \u548c 633\/640nm \u6fc0\u5149\u5668\u7684\u6d41\u5f0f\u7d30\u80de\u5100\u78ba\u5b9a\u3002\u73e0\u5206\u985e\u67d3\u6599\u7684\u6700\u5927\u767c\u5c04\u6ce2\u9577\u70ba 700 nm\u3002<\/p>\n<p>\u57fa\u65bc\u73e0\u5b50\u7684\u514d\u75ab\u6e2c\u5b9a\u985e\u4f3c\u65bc\u593e\u5fc3 ELISA \u7684\u539f\u7406\uff0c\u6bcf\u500b\u73e0\u5b50\u7fa4\u8207\u6355\u7372\u6a23\u54c1\u4e2d\u611f\u8208\u8da3\u7684\u86cb\u767d\u8cea\uff08\u4f8b\u5982\u7d30\u80de\u56e0\u5b50\uff09\u7684\u7279\u7570\u6027\u6355\u7372\u6297\u9ad4\u5076\u806f\u3002\u901a\u904e\u91dd\u5c0d\u86cb\u767d\u8cea\u4e8c\u7d1a\u8868\u4f4d\u7684\u751f\u7269\u7d20\u5316\u6297\u9ad4\u6aa2\u6e2c\u6355\u7372\u7684\u5206\u6790\u7269\u7684\u91cf\uff0c\u7136\u5f8c\u9032\u884c\u93c8\u9ef4\u89aa\u548c\u7d20-R-\u85fb\u7d05\u86cb\u767d\u8655\u7406\u3002\u73e0\u5b50\u4e0a\u7684 R-\u85fb\u7d05\u86cb\u767d\u7684\u87a2\u5149\u5f37\u5ea6\u5728\u6d41\u5f0f\u7d30\u80de\u5100\u4e0a\u9032\u884c\u91cf\u5316\u3002\u901a\u904e\u5c07\u87a2\u5149\u4fe1\u865f\u8207\u7531\u5df2\u77e5\u6fc3\u5ea6\u7684\u5206\u6790\u7269\u7684\u9023\u7e8c\u7a00\u91cb\u7522\u751f\u7684\u6a19\u6e96\u66f2\u7dda\u7684\u87a2\u5149\u4fe1\u865f\u9032\u884c\u6bd4\u8f03\uff0c\u53ef\u4ee5\u7372\u5f97\u6a23\u54c1\u4e2d\u611f\u8208\u8da3\u7684\u86cb\u767d\u8cea\u7684\u6fc3\u5ea6\u3002\u6e2c\u5b9a\u7a0b\u5e8f\u5305\u62ec 60 \u5206\u9418\u7684\u6297\u539f\u548c\u6355\u7372\u6297\u9ad4\u5076\u806f\u73e0\u5b75\u80b2\u6b65\u9a5f\u300130 \u5206\u9418\u7684\u751f\u7269\u7d20\u5316\u6aa2\u6e2c\u5b75\u80b2\u6b65\u9a5f\u548c 20 \u5206\u9418\u7684\u93c8\u9ef4\u89aa\u548c\u7d20 PE \u5b75\u80b2\u6b65\u9a5f\u3002<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/i.shgcdn.com\/2ce71d47-87eb-4118-85af-7cdd592051fb\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"\" \/><\/p>\n<p>\u6a23\u54c1\u5f62\u5f0f:<\/p>\n<p>Cell Culture Supernatants\u3001Plasma\u3001Serum\u3001Tissue Lysates\u3001 Cell Lysates<\/p>\n<p>&nbsp;<\/p>\n<p>Features &amp; Benefits<\/p>\n<ul>\n<li>Analyze: Multiplex 2-24 analytes in a single sample!<\/li>\n<li>Fast: 2 hour protocol!<\/li>\n<li>Sensitive: Measure as low as &lt;10 pg\/ml of each analyte<\/li>\n<li>Dynamic: Lower limit &lt; 20 pg\/mL | Upper limit &gt; 5,000 pg\/mL<\/li>\n<li>High Precision Intra-assay CV: &lt; 10% | Inter-assay CV: &lt; 20%<\/li>\n<li>Low Volumes: Use as little as 15\u03bcl of sample<\/li>\n<li>Validated: All assays fully tested for cross-reactivity in our lab<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<h2>Multiplex vs. Luminex?<\/h2>\n<p>The Assay Genie Multiplex ELISA gives excellent performance versus Luminex technology. The Human TH1\/TH2\/TH17 7-Plex ELISA and a Luminex multiplex assay were used to assay cell culture supernatant samples from human PBMCs treated with various reagents.<\/p>\n<p><img decoding=\"async\" src=\"https:\/\/i.shgcdn.com\/de3799a1-f433-44f3-9d9d-87a32a8c7bfb\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"GeniePlex versus Luminex data\" \/><\/p>\n<p>&nbsp;<\/p>\n<h2>Over 400 Analytes to measure!<\/h2>\n<ul>\n<li>Comprehensive Choice of Targets<\/li>\n<li>Up to 400 assays &amp; custom formats available for human, mouse, rat, porcine, canine and primates<\/li>\n<li>Wide Range of Sample Types<\/li>\n<li>Cell culture supernatants, saliva, plasma, cell\/tissue lysates, serum, BALF, pleural and peritoneal fluids &amp; more<\/li>\n<li>Multiple Formats: 32-well and 96-well sizes available<\/li>\n<li>Key Premixed Panels<\/li>\n<li>We have designed key panels to measure Cytokines, Chemokines, Inflammation, Th1\/Th2 &amp; Th1\/Th2\/Th17<\/li>\n<\/ul>\n<p><img decoding=\"async\" loading=\"lazy\" class=\"\" src=\"https:\/\/i.shgcdn.com\/1245b173-8391-4665-bdd3-4ae9642e8711\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"\" width=\"512\" height=\"274\" \/><\/p>\n<p>&nbsp;<\/p>\n<table class=\"shogun-table\">\n<thead class=\"shogun-table-column-container\">\n<tr>\n<td class=\"shogun-table-column\">Step<\/td>\n<td class=\"shogun-table-column\">Protocol<\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-3f5d5d84-756d-494f-8f35-6979d5ad41ad\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>1.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-765572dc-e592-4bb0-9ae9-8fe615b5aad5\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Prepare the filter plate template. Mark the standard, sample and blank wells. Standards and samples should be run in duplicates or triplicates. If the whole plate will not be used, seal the unused well with a plate seal.<\/p>\n<p>IMPORTANT: Place the filter plate on top of the filter plate lid during the entire assay process to prevent touching the plate bottom on any surface.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-92bd2045-61c8-489d-a4c2-a94dedb7a7b9\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>2.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-d843235e-cd08-4a60-b127-c5d92eac52dc\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Vortex working bead suspension for 15 seconds.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-ea3f5091-3502-4929-bd42-af22a7175b05\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>3.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-909e3e4a-e57e-43fc-b0db-24a2e0505a22\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Add 45 \u00b5L of capture bead working suspension to each well. NOTE: Save the remaining capture bead working suspension and store at 2-8\u00b0C with light protection. It can be used for setting up acquisition parameters on the flow cytometer.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-cd65226a-19d5-4a88-badf-31685d96fc30\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>4.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-d5883953-a913-4a12-8377-44832c16b97f\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove buffer in the wells by using the \u201cflow-through\u201c Filter Plate Washer connected to a vacuum source that has been adjusted according to the Filter Plate Washer Instructions.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-6082e88b-1018-4d8a-a186-596d2c7d0efd\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>5.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-1070ad18-eaff-4c52-992e-d8ccebe820b5\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove buffer in the wells by using the \u201cflow-through\u201c Filter Plate Washer connected to a vacuum source that has been adjusted according to the Filter Plate Washer Instructions.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-1cf52862-0c98-4752-b5cf-d31acea248ed\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>6.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-5789d6f0-6f19-4982-adad-08c5edb1ad1d\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Add 30 \u00b5L of CCS, SPB or TL Assay Buffer to each sample well.<\/p>\n<p>NOTE: Cell culture supernatant samples can be run without diluting in Assay Buffer if very low levels (less than 20 pg\/mL) of cytokines are expected. If it is the case, skip this step and add 45 \u00b5L of cell culture supernatant samples to each sample well in Step 7.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-0fd34b2d-d76d-4d23-b902-34d328be7d51\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>7.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-b9ae9959-101f-45b3-b125-124d09af5154\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Add 15 \u00b5L of samples to each sample well. Add 45 \u00b5L of standards to each standard well. Cover the plate with a plate seal.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-30daa525-1bfb-4769-8b80-42f48c1adb1c\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>8.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-414103d2-561c-4815-ba7f-37b2ef71f839\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Incubate on the shaker (set at 700 rpm) for 60 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-d84ec85e-101c-4f93-80ce-0731e1d36035\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>9.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-fb8e62dd-8c67-40cb-bbfe-d0e0498362f3\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer connected to a vacuum source.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-371f5841-094f-4957-8d8e-020d8f61508e\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>10.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-67d9fc33-86b0-428b-95e3-a9d3831877ed\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove solutions in the wells by using the Filter Plate Washer connected to a vacuum source.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-cbabb8c4-3839-4118-80ff-858dea099764\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>11.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-3af20fb9-c331-4fe9-8660-5423b4bb9a27\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Wash the wells three times with 100\u00b5L 1x Wash Buffer using the Filter Plate Washer.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-f13dee94-8ce8-496c-8bc1-d3b286d919d9\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>12.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-8c2e6d37-94eb-45fd-bb62-0572bba87522\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Gently tap the plate bottom onto several layers of paper towels to remove residual buffer on the plate bottom after the last wash.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-de30ec8f-59ac-4a87-9e7c-010aae21b2ff\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>13.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-09fc98ce-3b0d-4e30-8f09-70247a0eca36\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Add 25\u00b5L of biotinylated antibody working solution to each well. Cover the plate with a plate seal.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-8add195e-080f-445f-9d2a-1e0b13d5d322\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>14.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-52f42d96-d3d5-47cb-8f27-e4c28a94936d\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Incubate on the shaker (set at 700 rpm) for 30 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-68004d83-e58e-4a06-9419-e43310783fa3\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>15.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-2dbb0899-ef27-4ba7-8018-8c28c5398484\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer. Wash the wells three times with 100 \u00b5L 1x Wash Buffer using the Filter Plate Washer.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-de858682-ba7f-48e2-8e9a-45f2d701d2fe\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>16.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-4a561efb-2337-4a9e-b625-ea40e0fb6634\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Gently tap the plate bottom onto several layers of paper towels to remove residual buffer on the plate bottom after the last wash.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-12da7ae6-9700-48c8-a63d-da04bb0bf0f6\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>17.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-0f7be6dc-f0d9-4ff9-bdf8-1d1728a435f2\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Add 25\u00b5L of streptavidin-PE working solution to each well. Cover the plate with a plate seal.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-42d4f3e0-e1c9-414f-8be7-47b46aaef682\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>18.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-b7770f34-fd52-4cc2-b3f9-f733ed1c4a76\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Incubate on the shaker (set at 700 rpm) for 20 min at room temperature. Protect from light by wrapping the filter plate in aluminum foil.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-0e8f3737-a24d-411e-b2c9-8262438bd26a\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>19.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-1c27bb82-94e1-47c3-9ef5-e5f80a56203a\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Remove the plate seal. Remove solutions in the wells by using the Filter Plate Washer. Wash the wells twice with 100 \u00b5L 1x Wash Buffer.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-4fc7d5c6-14dc-449a-88da-0b394239a959\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>20.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-b086f812-3cf9-4278-9b2d-18bb87235599\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Gently tap the plate bottom onto several layers of paper towels to remove residual solution. Add 150\u00b5L to 300\u00b5L of 1x Reading Buffer to each well depending on the sample loading mechanism of a flow cytometer to re-suspend the beads. Cover the plate with a plate seal.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<tr class=\"shogun-table-row-container\">\n<td class=\"shogun-table-row\">\n<div id=\"s-b553f489-6894-4c24-9c3d-41ec398ae6ac\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>21.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<td class=\"shogun-table-row\">\n<div id=\"s-4f181126-bab1-4e14-a1ec-59ad381df8d1\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<p>Place the plate on the microtiter shaker and shake for 30 seconds at 700 rpm.<\/p>\n<p>NOTE: If the flow cytometer has no 96-well plate loader and more than 200 \u00b5L of 1x Reading Buffer is needed to re-suspend the beads, do not shake the plate. Re-suspend the beads in each well by pipetting up and down 6\u20138 times with a P200 pipette then transfer to a test tube for acquisition. Remove the plate seal. Read on a flow cytometer.<\/p>\n<\/div>\n<\/div>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><img decoding=\"async\" src=\"https:\/\/i.shgcdn.com\/56bae9ed-13ff-4cdb-8db6-d81faef2d5e8\/-\/format\/auto\/-\/preview\/3000x3000\/-\/quality\/lighter\/\" alt=\"\" \/><\/p>\n<div id=\"s-4469aaa2-a7c5-4730-984e-9d39cd86328c\" class=\"shg-c shg-align-left\">\n<div class=\"shogun-heading-component\" data-animations=\"[&quot;&quot;]\">\n<h3>Support Resources<\/h3>\n<\/div>\n<\/div>\n<div id=\"s-c7425d21-47fd-4294-9905-dc1bff2dc564\" class=\"shg-c \">\n<div class=\"shg-row\" data-col-grid-mode-on=\"\">\n<div class=\"shg-c-lg-6 shg-c-md-6 shg-c-sm-6 shg-c-xs-12\">\n<div id=\"s-5b539a65-4e86-42a8-a2d7-6672004e246a\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<ul>\n<li><a href=\"https:\/\/www.assaygenie.com\/human-multiplex-cytokine-elisa-panel\">Human Multiplex Cytokine Panels<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/human-multiplex-inflammation-elisa-panels\">Human Multiplex Inflammation ELISA Panels<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/mouse-multiplex-cytokine-elisa-panel\">Mouse Multiplex Cytokine ELISA Panels<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/mouse-multiplex-inflammation-elisa-panels\">Mouse Multiplex Inflammation ELISA Panels<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/rat-multiplex-elisa-panels\">Rat Multiplex ELISA<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/what-are-cytokines\">What are Cytokines?<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"shg-c-lg-6 shg-c-md-6 shg-c-sm-6 shg-c-xs-12\">\n<div id=\"s-e2b74570-86be-46c3-8668-dd9725344163\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<ul>\n<li><a href=\"https:\/\/www.assaygenie.com\/multiplex-elisa-troubleshooting\">Multiplex Troubleshooting<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/content\/ELISA%20Genie\/AM\/GeniePlex%20Technical%20Manual.pdf\">Technical Manual<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/canine-multiplex-elisa\">Canine Multiplex ELISA<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/cytokine-storm-assays\">Cytokine Storms Assays<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/multiplex-elisa-kits-jp\">\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9 ELISA<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<div id=\"s-5688415c-23ac-4059-a85f-569ea541d112\" class=\"shg-c shg-align-left\">\n<div class=\"shogun-heading-component\" data-animations=\"[&quot;&quot;]\">\n<h3>Support Resources<\/h3>\n<\/div>\n<\/div>\n<div id=\"s-6d3351ed-556c-4ed3-b0cb-e84e22871dba\" class=\"shg-c \">\n<div class=\"shg-row\" data-col-grid-mode-on=\"\">\n<div class=\"shg-c-lg-6 shg-c-md-6 shg-c-sm-6 shg-c-xs-12\">\n<div id=\"s-b5c75fdd-c6ca-4786-a0e0-9edd7d1d0d3b\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<ul>\n<li><a href=\"https:\/\/www.assaygenie.com\/101-elisa-troubleshooting-tips\/\">101 ELISA Troubleshooting Tips<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/101-western-blot-troubleshooting-tips\">101 Western Blot Protocol Troubleshooting Tips<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/cell-based-elisa-kits\">Cell Based ELISA Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/multiplex-elisa-kits\">Multiplex ELISA Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/cytokine-elisa-kits\">Cytokine ELISA Kits<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<div class=\"shg-c-lg-6 shg-c-md-6 shg-c-sm-6 shg-c-xs-12\">\n<div id=\"s-f87429d5-1191-4b5b-9cab-dc6485d78a7c\" class=\"shg-c \">\n<div class=\"shg-rich-text shg-theme-text-content\" data-animations=\"[&quot;&quot;]\">\n<ul>\n<li><a href=\"https:\/\/www.assaygenie.com\/covid19-research-kits-tools\">COVID-19 Research Tools<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/elispot-assay\">ELIspot Assays<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/food-safety-elisa-kits\">Food Safety ELISA Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/hormone-peptide-small-molecule-elisa-kits\">Hormone &amp; Small Molecule ELISA Kits<\/a><\/li>\n<li><a href=\"https:\/\/www.assaygenie.com\/antibodies\">Antibodies<\/a><\/li>\n<\/ul>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>GeniePlex\u591a\u91cd\u514d\u75ab\u6aa2\u6e2c\u6280\u8853 (Multiplex ELISA Array, Assay Genie M [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":31205,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[951,952,950],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31204"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=31204"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31204\/revisions"}],"predecessor-version":[{"id":31211,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/31204\/revisions\/31211"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/31205"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=31204"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=31204"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=31204"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}