{"id":29325,"date":"2021-09-13T10:51:23","date_gmt":"2021-09-13T02:51:23","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=29325"},"modified":"2022-07-19T13:41:15","modified_gmt":"2022-07-19T05:41:15","slug":"cultrex-bme%e5%9f%ba%e5%ba%95%e8%86%9c%e6%8f%90%e5%8f%96%e7%89%a9-pathclear-ledv-free%e8%b2%a8%e8%99%9f3433-001-01","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=29325","title":{"rendered":"Cultrex\u00ae BME\u57fa\u5e95\u819c\u63d0\u53d6\u7269 | PathClear LEDV free\u8ca8\u865f3532-005-02"},"content":{"rendered":"

Cultrex\u00ae BME\u57fa\u5e95\u819c\u63d0\u53d6\u7269 |\u00a0 \u8ca8\u865f3532-005-02<\/a><\/span><\/p>\n

Cultrex Basement Membrane Extract, Type 2, Pathclear<\/p>\n

\"\u00e7\u0094\u00a2\u00e5\u0093\u0081\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"Cultrex\u00ae\u57fa\u5e95\u819c\u63d0\u53d6\u7269\uff08Cultrex\u00ae Basement Membrane Extract , BME\uff09\u662f\u5f9eEngelbreth-Holm-Swarm\uff08Engelbreth-Holm-Swarm , EHS\uff09\u816b\u7624\u7d14\u5316\u7684\u57fa\u5e95\u819c(basement membrane)\u7684\u53ef\u6eb6\u5f62\u5f0f\u3002\u8a72\u63d0\u53d6\u7269\u63d0\u4f9b\u4e86\u5929\u7136\u7684\u7d30\u80de\u5916\u57fa\u8cea\u6c34\u51dd\u81a0(extracellular matrix hydrogel)\uff0c\u8a72\u51dd\u81a0\u572837\u00b0C\u805a\u5408\u5f62\u6210\u91cd\u7d44\u7684\u57fa\u5e95\u819c\u3002\u57fa\u5e95\u819c(Basement membranes)\u662f\u7279\u6b8a\u7684\u7d30\u80de\u5916\u57fa\u8cea(extracellular matrix)\u7684\u9023\u7e8c\u7247\uff0c\u5f62\u6210\u5167\u76ae(endothelial)\uff0c\u4e0a\u76ae(epithelial)\uff0c\u808c\u8089(muscle)\u6216\u795e\u7d93\u5143\u7d30\u80de(neuronal cells)\u8207\u5176\u76f8\u9130\u57fa\u8cea\u4e4b\u9593\u7684\u754c\u9762\uff0c\u4e26\u901a\u904e\u5f71\u97ff\u7d30\u80de\u7c98\u9644(cell adhesion)\uff0c\u9077\u79fb(migration)\uff0c\u589e\u6b96(proliferation)\u548c\u5206\u5316(differentiation)\u5728\u7d44\u7e54\u7d44\u7e54\u4e2d\u767c\u63ee\u91cd\u8981\u4f5c\u7528\u3002\u57fa\u5e95\u819c\u63d0\u53d6\u7269\uff08Cultrex\u00ae Basement Membrane Extract , BME\uff09\u7684\u4e3b\u8981\u6210\u5206\u5305\u62ec\u5c64\u7c98\u9023\u86cb\u767d(laminin)\uff0c\u81a0\u539f\u86cb\u767dIV(collagen IV)\uff0c\u52d5\u86cb\u767d(entactin)\u548c\u786b\u9178\u4e59\u9170\u809d\u7d20\u86cb\u767d\u805a\u91a3(heparan sulfate proteoglycans)\u3002<\/p>\n

\"\"<\/p>\n

Cultrex\u00ae BME Endothelial Tube Formation.<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"<\/p>\n

Cultrex\u00aeRGF BME\u662f\u4e00\u7a2e\u5df2\u958b\u767c\uff0c\u751f\u7522\u4e26\u7b26\u5408\u4e00\u822c\u7d30\u80de\u57f9\u990a\u61c9\u7528\u8981\u6c42\u7684\u57fa\u5e95\u819c\u57fa\u8cea\u3002\u5c0dCultrex\u00aeRGF BME\u9032\u884c\u4e86\u8655\u7406\uff0c\u4ee5\u6e1b\u5c11\u8207\u57fa\u8cea\u76f8\u95dc\u7684\u751f\u9577\u56e0\u5b50\uff0c\u4e26\u63d0\u4f9b\u4e86\u66f4\u660e\u78ba\u7684\u6a21\u578b\u7cfb\u7d71\u3002<\/p>\n

\u8cea\u91cf\u63a7\u5236(Quality control)\u898f\u683c\uff1a<\/strong><\/span><\/p>\n

    \n
  1. \u4f7f\u7528\u5c0f\u9f20\u6297\u9ad4\u751f\u7522\uff08mouse antibody production , MAP\uff09\u6e2c\u8a66\uff0c\u5e38\u898f\u7be9\u67e5\u5c0f\u9f20\u83cc\u843d\u7684\u75c5\u539f\u9ad4\u3002<\/li>\n
  2. Engelbreth-Holm-Swarm\uff08Engelbreth-Holm-Swarm , EHS\uff09\u816b\u7624\u4e0d\u542bLDEV\u3002<\/li>\n
  3. PathClear\u00ae\u2013\u901a\u904ePCR\u6aa2\u6e2c\u70ba\u9670\u6027\u768417\u7a2e\u7d30\u83cc\u548c\u75c5\u6bd2\u682a\uff0c\u901a\u5e38\u5305\u542b\u5728\u5c0f\u9f20\u6297\u9ad4\u751f\u7522\uff08mouse antibody production , MAP\uff09\u6e2c\u8a66\u4e2d\uff0c\u53e6\u5916\u9084\u670913\u7a2e\u9f20\u985e\u50b3\u67d3\u539f(murine infectious agents)\uff0c\u5305\u62ecLDEV\uff0c\u5171\u8a0831\u7a2e\u751f\u7269\u548c\u75c5\u6bd2\u3002<\/li>\n
  4. \u6839\u64daUSP\u7121\u83cc\u6e2c\u8a66\u6307\u5357\uff0c\u572837\u00b0C\u4e0b\u5b75\u80b214\u5929\u5f8c\u672a\u6aa2\u6e2c\u5230\u7d30\u83cc\u6216\u771f\u83cc\u751f\u9577\u3002,<\/li>\n
  5. LAL\u6e2c\u5b9a(Limulus Amoebocyte Lysate) \u7684\u5167\u6bd2\u7d20\u6fc3\u5ea6\u22648 EU \/ ml\u3002<\/li>\n
  6. \u86cb\u767d\u8cea\u6fc3\u5ea6\u70ba12 \u2013 18 mg \/ ml\u3002PCR\u672a\u6aa2\u6e2c\u5230mycoplasma\u6c61\u67d3\u3002<\/li>\n
  7. \u5728\u4e0d\u542b\u915a\u7d05\u7684Dulbecco\u6539\u826f\u578bEagle\u57f9\u990a\u57fa\uff08DMEM\uff09\u4e2d\u63d0\u4f9b\uff0c\u542b10 \u00b5g \/ ml\u786b\u9178\u6176\u5927\u9709\u7d20(gentamicin sulfate)\u3002<\/li>\n
  8. \u51dd\u81a0\u7a69\u5b9a\u6027(Gel stability tested)\u572837\u00b0C\u4e0b\u6e2c\u8a6614\u5929\u3002<\/li>\n<\/ol>\n

    \u78ba\u5b9a\u6bcf\u500b\u6279\u6b21\u7684\u751f\u7269\u6d3b\u6027(Biological activity)\uff1a\u7ba1\u5f62\u6210\u5206\u6790(Tube formation assay)\u2013 BME\u4fc3\u9032\u4eba\uff08HBMVEC; HUVEC\uff09\u6216\u5c0f\u9f20\uff08SVEC4-10\uff09\u5167\u76ae\u7d30\u80de\u5f62\u6210\u6bdb\u7d30\u8840\u7ba1\u6a23\u7d50\u69cb\u3002<\/span><\/strong><\/p>\n

    \u5f15\u7528\u6587\u737b15+<\/sup><\/a><\/p>\n

    \"Assessment<\/p>\n

    \u76f8\u95dc\u7522\u54c1\u00a0\u5f57\u661f\u5206\u6790\u5957\u7d44<\/a>\u00a0|\u00a0\u5f57\u661f\u5206\u6790\u5c08\u7528\u96fb\u6cf3\u8a2d\u5099<\/a>\u00a0|\u00a0hOGG1 DNA\u4fee\u5fa9\u9176<\/a>\u00a0|\u00a0\u7e3d\u6297\u6c27\u5316\u80fd\u529b\u5206\u6790<\/a>\u00a0|\u00a0DNA\u96fb\u6cf3\u69fd<\/a>\u00a0|\u00a0\u9280\u67d3\u8a66\u5291\u7d44<\/a><\/p>\n

    \"\u00e4\u00bd\u00bf\u00e7\u0094\u00a8\u00e6\u0096\u00b9\u00e6\u00b3\u0095A\"\u539a\u51dd\u81a0\u6cd5(Thick Gel Method)\uff1a<\/p>\n

    1. \u5982\u4e0a\u6240\u8ff0\u89e3\u51cd Cultrex BME\u3002<\/p>\n

    2. \u7de9\u6162\u4e0a\u4e0b\u5439\u6253\u6eb6\u6db2\u6df7\u5408 Cultrex BME\uff1b\u5c0f\u5fc3\u4e0d\u8981\u5f15\u5165\u6c23\u6ce1\u3002<\/p>\n

    3. \u5c07\u6bcf\u5e73\u65b9\u5398\u7c73 200-300 \u03bcL \u79fb\u6db2\u5230\u751f\u9577\u8868\u9762\u4e0a\u3002<\/p>\n

    4. \u5c07\u5857\u5c64\u7269\u9ad4\u5728 37 \u00b0C \u4e0b\u653e\u7f6e 30 \u5206\u9418\u3002<\/p>\n

    5. \u5857\u5c64\u7269\u9ad4\u5373\u53ef\u4f7f\u7528\u3002<\/p>\n

    \"\u00e4\u00bd\u00bf\u00e7\u0094\u00a8\u00e6\u0096\u00b9\u00e6\u00b3\u0095B\"\u8584\u5c64\u6cd5\uff08Thin Layer Method\u00a0\u975e\u81a0\u51dd\uff09\uff1a<\/p>\n

    1. \u5982\u4e0a\u6240\u8ff0\u89e3\u51cd Cultrex BME\u3002<\/p>\n

    2. \u7de9\u6162\u4e0a\u4e0b\u5439\u6253\u6eb6\u6db2\u6df7\u5408 Cultrex BME\uff1b\u5c0f\u5fc3\u4e0d\u8981\u5f15\u5165\u6c23\u6ce1\u3002<\/p>\n

    3. \u5728\u51b7\u7684\u7121\u8840\u6e05\u57f9\u990a\u57fa\u4e2d\u5c07 Cultrex BME \u7a00\u91cb\u81f3\u6240\u9700\u6fc3\u5ea6\u3002\u63a8\u85a6 1:100 \u7a00\u91cb\u7528\u65bc\u539f\u4ee3\u7d30\u80de\u7684\u7e41\u6b96\u3002\u53ef\u80fd\u9700\u8981\u6839\u64da\u60a8\u7684\u61c9\u7528\u78ba\u5b9a\u6700\u4f73\u5857\u5c64\u6fc3\u5ea6\u3002<\/p>\n

    4. \u6dfb\u52a0\u8db3\u91cf\u7684\u6eb6\u6db2\u4ee5\u8986\u84cb\u6574\u500b\u751f\u9577\u8868\u9762\u7a4d\u3002\u5efa\u8b70\u9ad4\u7a4d\u70ba 300 \u00b5L\/cm2\u3002<\/p>\n

    5. \u5728\u5ba4\u6eab\u4e0b\u5b75\u80b2\u5857\u5c64\u7269\u9ad4\u4e00\u5c0f\u6642\u3002<\/p>\n

    6. \u5438\u51fa\u5305\u88ab\u6eb6\u6db2\u4e26\u7acb\u5373\u5e73\u677f\u7d30\u80de\u3002\u4e0d\u8981\u8b93\u5857\u5c64\u8868\u9762\u8b8a\u4e7e\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
    \n

    \u7522\u54c1\u578b\u865f<\/b><\/p>\n<\/td>\n

    		\n

    \u7522\u54c1\u540d\u7a31<\/b><\/p>\n<\/td>\n

    		\u5305\u88dd<\/b><\/td>\n<\/tr>\n
    \n

    3432-001-01
    \n3432-005-01
    \n3432-010-01<\/p>\n<\/td>\n

    		Cultrex\u00ae Basement Membrane Extract, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3433-001-01
    \n3433-005-01
    \n3433-010-01<\/p>\n<\/td>\n

    		Cultrex\u00ae Reduced Growth Factor Basement Membrane Extract, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3532-001-02
    \n3532-005-02
    \n3532-010-02<\/p>\n<\/td>\n

    		Cultrex\u00ae Basement Membrane Extract, Type 2, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3533-001-02
    \n3533-005-02
    \n3533-010-02<\/p>\n<\/td>\n

    		Cultrex\u00ae Reduced Growth Factor Basement Membrane Extract, Type 2, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3632-001-02
    \n3632-005-02
    \n3632-010-02<\/p>\n<\/td>\n

    		Cultrex\u00ae Basement Membrane Extract, Type 3, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3445-001-01
    \n3445-005-01
    \n3445-010-01<\/p>\n<\/td>\n

    		Cultrex\u00ae 3-D Culture Matrix\u2122 Reduced Growth Factor Basement Membrane Extract, PathClear\u00ae<\/td>\n1 ml
    \n5 ml
    \n2 \u00d7 5 ml<\/td>\n<\/tr>\n
    \n

    3415-001-03<\/p>\n<\/td>\n

    		Cultrex\u00ae Stem Cell Qualified Human Basement Membrane Extract, PathClear\u00ae<\/td>\n1 ml<\/td>\n<\/tr>\n
    \n

    3434-001-02
    \n3434-005-02<\/p>\n<\/td>\n

    		Cultrex\u00ae Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract, PathClear\u00ae<\/td>\n1 ml
    \n5 ml<\/td>\n<\/tr>\n
    \n

    3430-50-01<\/p>\n<\/td>\n

    		100X Phenol Red Solution<\/td>\n50 \u00b5l<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    Cultrex\u00ae BME\u57fa\u5e95\u819c\u63d0\u53d6\u7269 |\u00a0 \u8ca8\u865f3532-005-02 Cultrex Basement Me […]<\/p>\n","protected":false},"author":3,"featured_media":25596,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1012],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29325"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=29325"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29325\/revisions"}],"predecessor-version":[{"id":29331,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29325\/revisions\/29331"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/25596"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=29325"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=29325"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=29325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}