{"id":29280,"date":"2021-09-09T16:03:00","date_gmt":"2021-09-09T08:03:00","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=29280"},"modified":"2023-03-20T11:57:34","modified_gmt":"2023-03-20T03:57:34","slug":"d-luciferin-sodium-salt-%e8%9e%a2%e5%85%89%e7%b4%a0%e9%88%89%e9%b9%bd-a-substrate-for-luciferase%e8%b2%a8%e8%99%9f7902","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=29280","title":{"rendered":"D-Luciferin, Sodium Salt-\u87a2\u5149\u7d20\u9209\u9e7d | A substrate for luciferase\u8ca8\u865f7902 (abcam ab145164)"},"content":{"rendered":"

D-Luciferin, Sodium Salt-\u87a2\u5149\u7d20\u9209\u9e7d<\/a><\/span>\u00a0|\u00a0<\/strong>\u8ca8\u865f<\/strong>7902\u00a0\u00a0A substrate for luciferase\u00a0(abcam ab145164)<\/p>\n

\"\"<\/p>\n

\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly Luciferase)<\/strong>\u69cb\u6210\u4e86\u5404\u7a2e\u5206\u6790\u6280\u8853\u7684\u57fa\u790e\u3002\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176(Firefly Luciferase)<\/strong>\u4e0d\u7a69\u5b9a\uff0c\u5728\u5ba4\u6eab\u4e0b\u6703\u8fc5\u901f\u5931\u53bb\u6d3b\u6027\u3002\u9019\u5f71\u97ff\u8a66\u9a57\u9748\u654f\u5ea6\u548c\u7cbe\u6e96\u5ea6(precision)\uff0c\u4e26\u9650\u5236\u8a2d\u5099\u7684\u53ef\u884c\u6027\u3002\u70ba\u4e86\u514b\u670d\u9019\u4e9b\u969c\u7919\uff0cBioVision\u6700\u8fd1\u958b\u767c\u4e86StayBrite\u2122\u9ad8\u7a69\u5b9a\u6027\u87a2\u5149\u7d20\u9176((Firefly Luciferase))<\/strong>\uff0c\u5b83\u5229\u7528\u4e86\u907a\u50b3\u4fee\u98fe\u7684\u8b8a\u7a2e\uff08rLucHS\uff09\uff0c\u8a72\u8b8a\u9ad4\u884d\u751f\u81ea\u4e2d\u570b\u96f2\u5357\u7701\u7279\u6709\u7684\u679c\u81a0Dia\uff08\u87a2\u706b\u87f2\uff09\u7684\u87a2\u5149\u7d20\u9176\u3002\u5c0d\u5176\u9032\u884c\u4e86\u6539\u826f\uff0c\u4ee5\u4f7f\u5176\u8207Photinus pyralis\u7684\u6b63\u5e38\u8868\u578b\u76f8\u6bd4\u5177\u6709\u589e\u5f37\u7684\u7a69\u5b9a\u6027\uff0c\u5728\u66f4\u5bec\u6cdb\u4e14\u8207\u751f\u7406\u76f8\u95dc\u7684\u6709\u6548pH\u7bc4\u570d\u5167\u5177\u6709\u66f4\u597d\u7684\u9748\u654f\u5ea6\u548c\u6027\u80fd\u3002\u5728\u4f4e\u65bc\u301c8.2\u7684\u6240\u6709pH\u503c\u4e0b\uff0cBioVision\u7684rLucHS\u5747\u6bd4\u5e02\u552e\u7684Photinus\u87a2\u5149\u7d20\u9176\u6d3b\u8e8d\u5f97\u591a\uff0c\u4e26\u4e14\u5728\u5ba4\u6eab\u4e0b\u53ef\u7a69\u5b9a\u653e\u7f6e\u6578\u9031\uff0c\u572837\u00b0C\u4e0b\u53ef\u7a69\u5b9a\u653e\u7f6e60\u5206\u9418\u4ee5\u4e0a\u3002\u6d3b\u6027\u66f2\u7dda\u986f\u793aATP\u503c\u7684\u7dda\u6027\u6aa2\u6e2c\u7bc4\u570d\u4f4e\u81f310 fmol \/\u6e2c\u5b9a\u6cd5\uff081 nM\uff09\u3002 rLucHS\u7684\u6bd4\u6d3b\u6027\u70ba\u301c5 x 1011 RLU \/ mg\u86cb\u767d\u3002\u8a72\u6e2c\u5b9a\u53ef\u4ee5\u5b8c\u5168\u81ea\u52d5\u5316\u4ee5\u5be6\u73fe\u9ad8\u901a\u91cf\u4e26\u4e14\u975e\u5e38\u9748\u654f\u3002\u8a72\u6e2c\u5b9a\u6cd5\u7684\u9ad8\u9748\u654f\u5ea6\u975e\u5e38\u9069\u5408\u6aa2\u6e2c\u5404\u7a2e\u9176\u4fc3\u53cd\u61c9\u4e2dATP\u7684\u7522\u751f\u6216\u6d88\u8017\u3002<\/p>\n

\"\"<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"D-Luciferin\u00a0<\/strong>D-\u87a2\u5149\u7d20\u9209\u9e7d(StayBrite\u2122 D-Luciferin sodium salt\u00a0)\u662f\u87a2\u706b\u87f2\u87a2\u5149\u7d20\u9176\u7684\u6709\u7528\u5e95\u7269(substrate)\u3002 \u7576\u9176\u87a2\u5149\u7d20\u9176\u6c27\u5316\u6642\uff0c\u7522\u751f\u751f\u7269\u767c\u5149\u3002 D-\u87a2\u5149\u7d20\u53ef\u7528\u65bc\u6e2c\u5b9a\u8207\u611f\u8208\u8da3\u7684\u555f\u52d5\u5b50\u76f8\u9023\u4e4b\u87a2\u5149\u7d20\u9176\u57fa\u56e0\u7684\u8868\u9054\u3002 D-\u87a2\u5149\u7d20\u548c\u87a2\u5149\u7d20\u9176\u53ef\u7528\u65bc\u8a55\u4f30\u7d30\u80de\u6216\u751f\u7269\u5316\u5b78\u6e2c\u5b9a\u4e2dATP\u8868\u73fe\u3002\u5728\u6574\u500b\u751f\u7269\u6280\u8853\u9818\u57df\u7684\u61c9\u7528\u5305\u62ecATP\u5206\u6790(\u00a0ATP assays)\uff0c\u57fa\u56e0\u5831\u544a\u57fa\u56e0\u5206\u6790(\u00a0gene reporter assays)\uff0c\u9ad8\u901a\u91cf\u6e2c\u5e8f(gene reporter assays)\u548c\u5404\u7a2e\u6c61\u67d3\u5206\u6790(\u00a0contamination assays)\u3002\u5c0d\u65bc\u9ad4\u5167\u6210\u50cf(in vivo<\/i>\u00a0imaging)\uff0c\u5728\u6a21\u578b\u7cfb\u7d71\u4e2d\u6ce8\u5c04\u87a2\u5149\u7d20(\u00a0luciferin )\u53ef\u4ee5\u901a\u904e\u751f\u7269\u767c\u5149\uff0cnon-invasive\u76e3\u6e2c\u75be\u75c5\u9032\u5c55\u548c\/\u6216\u85e5\u7269\u7642\u6548(\u00a0drug efficacy )\u3002<\/span><\/p>\n

\"\"<\/p>\n

 <\/p>\n\n\n\n\n\n\n\n\n\n
Alternate Name<\/strong><\/td>\nAerosporin; Mastimyxin<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nWhite solid<\/td>\n<\/tr>\n
CAS #<\/strong><\/td>\n1405-20-5<\/td>\n<\/tr>\n
Molecular Formula<\/strong><\/td>\nC\u2085\u2085H\u2089\u2086N\u2081\u2086O\u2081\u2083 \u00b7 2H\u2082SO\u2084<\/td>\n<\/tr>\n
Molecular Weight<\/strong><\/td>\n1385.61<\/td>\n<\/tr>\n
Purity<\/strong><\/td>\n>6000 units\/mg<\/td>\n<\/tr>\n
Solubility<\/strong><\/td>\nH\u2082O (25 mg\/ml)<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0\"7902\"<\/a><\/strong><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
Name<\/td>\nCat. No.<\/td>\nSize<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-100<\/a><\/td>\n100 mg<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-1G<\/a><\/td>\n1 g<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-3<\/a><\/td>\n3 x 1 g<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-5<\/a><\/td>\n5 x 1 g<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-10G<\/a><\/td>\n10 g<\/td>\n<\/tr>\n
D-Luciferin, Potassium Salt<\/a><\/td>\n7903-10<\/a><\/td>\n10 x 1 g<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-100<\/a><\/td>\n100 mg<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-1G<\/a><\/td>\n1 g<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-3<\/a><\/td>\n3 x 1 g<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-5<\/a><\/td>\n5 x 1 g<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-10G<\/a><\/td>\n10 g<\/td>\n<\/tr>\n
D-Luciferin, Sodium Salt<\/a><\/td>\n7902-10<\/a><\/td>\n10 x 1 g<\/td>\n<\/tr>\n
D-Luciferin (Free acid)<\/a><\/td>\n2779-250<\/a><\/td>\n250 mg<\/td>\n<\/tr>\n
D-Luciferin (Free acid)<\/a><\/td>\n2779-500<\/a><\/td>\n500 mg<\/td>\n<\/tr>\n
D-Luciferin (Free acid)<\/a><\/td>\n2779-1000<\/a><\/td>\n1 g<\/td>\n<\/tr>\n
D-Luciferin (Free acid)<\/a><\/td>\n2779-5000<\/a><\/td>\n5 g<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

D-Luciferin, Sodium Salt-\u87a2\u5149\u7d20\u9209\u9e7d\u00a0|\u00a0\u8ca8\u865f7902\u00a0\u00a0A substrate fo […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1018,1019],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29280"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=29280"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29280\/revisions"}],"predecessor-version":[{"id":40310,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/29280\/revisions\/40310"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=29280"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=29280"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=29280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}