{"id":28828,"date":"2021-08-26T13:49:44","date_gmt":"2021-08-26T05:49:44","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28828"},"modified":"2022-07-19T13:54:22","modified_gmt":"2022-07-19T05:54:22","slug":"25-hydroxy-vitamin-d3-elisa%e8%a9%a6%e5%8a%91%e7%9b%92-25-hydroxy-vitamin-d3-elisa%e8%b2%a8%e8%99%9fkte60833","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28828","title":{"rendered":"25-hydroxy vitamin D3 ELISA\u8a66\u5291\u76d2| 25-hydroxy vitamin D3 ELISA\u8ca8\u865fKTE60833"},"content":{"rendered":"

25-hydroxy vitamin D3 ELISA\u8a66\u5291\u76d2| \u00a0<\/strong>25-hydroxy vitamin D3 ELISA\u8ca8\u865f<\/strong>KTE60833<\/a><\/span><\/p>\n

Human 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit<\/p>\n

\"abbkine\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

\u9aa8\u5316\u4e8c\u9187(Calcifediol)\u662f\u4e00\u7a2e\u524d\u6fc0\u7d20\uff0c\u7531\u809d\u81df\u4e2d\u7dad\u751f\u7d20 D3\uff08\u81bd\u9223\u5316\u9187\uff09\u7684\u7fa5\u57fa\u5316\u7522\u751f\u3002\u7136\u5f8c\u9aa8\u5316\u4e8c\u9187(Calcifediol)\u5728\u814e\u81df\u4e2d\uff08\u901a\u904e\u9176 25(OH)D-1\u03b1-\u7fa5\u5316\u9176\uff09\u8f49\u5316\u70ba\u9aa8\u5316\u4e09\u9187 (1,25-(OH)2D3)\uff0c\u4e00\u7a2e\u662f\u7dad\u751f\u7d20 D \u6d3b\u6027\u5f62\u5f0f\u7684\u985e\u56fa\u9187\u6fc0\u7d20\u3002\u5b83\u4e5f\u53ef\u4ee5\u662f\u901a\u904e 24-\u7fa5\u57fa\u5316\u4f5c\u7528\u5728\u814e\u81df\u4e2d\u8f49\u5316\u70ba 24-\u7fa5\u57fa\u9aa8\u5316\u4e8c\u9187\u3002\u5728\u91ab\u5b78\u4e0a\uff0c\u9aa8\u5316\u9187\u7684\u8840\u6fc3\u5ea6\u88ab\u8a8d\u70ba\u662f\u7dad\u751f\u7d20 D \u72c0\u614b\u7684\u6700\u4f73\u6307\u6a19\u3002\u9019\u662f\u6700\u654f\u611f\u7684\u63aa\u65bd\uff0c\u5118\u7ba1\u5c08\u5bb6\u547c\u7c72\u63d0\u9ad8\u4e0d\u540c\u5be6\u9a57\u5ba4\u7684\u6a19\u6e96\u5316\u548c\u53ef\u91cd\u8907\u6027\u3002\u6b63\u5e38\u7bc4\u570d\u56e0\u591a\u7a2e\u56e0\u7d20\u800c\u7570\uff0c\u5305\u62ec\u5e74\u9f61\u548c\u5730\u7406\u4f4d\u7f6e\u3002\u5df2\u5efa\u8b70 20\u2013150 nmol\/L \u7684\u5ee3\u6cdb\u53c3\u8003\u7bc4\u570d\uff0c\u800c\u5e7e\u9805\u7814\u7a76\u5df2\u5c07\u4f4e\u65bc 80 nmol\/L \u7684\u6c34\u5e73\u5b9a\u7fa9\u70ba\u7dad\u751f\u7d20 D \u7f3a\u4e4f\u7684\u6307\u793a<\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\"This Human 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit employs a two-site sandwich ELISA to quantitate 25 HVD3 in samples. An antibody specific for 25 HVD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any25 HVD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 25 HVD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 25 HVD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n
Product name<\/strong><\/td>\nHuman 25-hydroxy vitamin D3 (25 HVD3) ELISA Kit<\/a><\/td>\n<\/tr>\n
Reactivity<\/strong><\/td>\nHuman<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nELISA<\/td>\n<\/tr>\n
Detection method<\/strong><\/td>\nColorimetric<\/td>\n<\/tr>\n
SampleType<\/strong><\/td>\nCell culture supernatants, Other biological fluids, Plasma, Serum<\/td>\n<\/tr>\n
Assay type<\/strong><\/td>\nSandwich ELISA (quantitative)<\/td>\n<\/tr>\n
Assay duration<\/strong><\/td>\nMultiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.<\/td>\n<\/tr>\n
Alternative<\/strong><\/td>\n25 HVD3<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u4f7f\u7528\u6ce8\u610f\u4e8b\u9805<\/p>\n

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  • \u8acb\u52ff\u6df7\u7528\u4e0d\u540c\u8a66\u5291\u76d2\u4e2d\u7684\u7d44\u5206\uff0c\u6216\u5728\u8a66\u5291\u76d2\u5230\u671f\u65e5\u671f\u4e4b\u5f8c\u4f7f\u7528\u8a66\u5291\u3002<\/li>\n
  • \u6253\u958b\u4e4b\u524d\uff0c\u8b93\u6240\u6709\u8a66\u5291\u81f3\u5c11\u52a0\u5ba4\u6eab30\u5206\u9418\u3002<\/li>\n
  • \u7528\u8a66\u5291\u9810\u6e05\u6d17\u79fb\u6db2\u5668\u5438\u982d\uff0c\u70ba\u6bcf\u500b\u6a23\u54c1\uff0c\u6a19\u6e96\u6db2\u548c\u8a66\u5291\u4f7f\u7528\u65b0\u9bae\u7684\u79fb\u6db2\u5668\u5438\u982d\uff0c\u4ee5\u514d\u6c61\u67d3\u3002<\/li>\n
  • \u672a\u4f7f\u7528\u7684\u5b54\u5fc5\u9808\u5728\u63d0\u4f9b\u7684\u5bc6\u5c01\u888b\u4e2d\u65bc4\u00b0C\u4e7e\u71e5\u3002<\/li>\n
  • \u5fb9\u5e95\u6df7\u5408\u5c0d\u65bc\u7d50\u679c\u975e\u5e38\u91cd\u8981\u3002\u5efa\u8b70\u6bcf10\u5206\u9418\u4f7f\u7528\u4f4e\u983b\u632f\u76ea\u5668\u6216\u7a0d\u7a0d\u6416\u52d5\u624b\u3002<\/li>\n
  • \u5efa\u8b70\u6240\u6709\u6a23\u54c1\u548c\u6a19\u6e96\u54c1\u4e00\u5f0f\u5169\u4efd\u6216\u4e00\u5f0f\u4e09\u4efd\u9032\u884c\u6e2c\u5b9a\u3002<\/li>\n<\/ul>\n

     <\/p>\n

     <\/p>\n

     <\/p>\n","protected":false},"excerpt":{"rendered":"

    25-hydroxy vitamin D3 ELISA\u8a66\u5291\u76d2| \u00a025-hydroxy vitamin D3 […]<\/p>\n","protected":false},"author":3,"featured_media":24540,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[452],"tags":[1055],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28828"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28828"}],"version-history":[{"count":1,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28828\/revisions"}],"predecessor-version":[{"id":28829,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28828\/revisions\/28829"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/24540"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28828"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28828"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28828"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}