{"id":28756,"date":"2021-08-24T18:36:45","date_gmt":"2021-08-24T10:36:45","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28756"},"modified":"2022-07-19T13:55:57","modified_gmt":"2022-07-19T05:55:57","slug":"%e7%94%9f%e7%89%a9%e7%b4%a0%e5%8c%96%e8%be%a3%e6%a0%b9%e9%81%8e%e6%b0%a7%e5%8c%96%e7%89%a9%e9%85%b6-biotinylated-horseradish-peroxidase%e8%b2%a8%e8%99%9fp1473-5","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28756","title":{"rendered":"\u751f\u7269\u7d20\u5316\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 | Biotinylated Horseradish Peroxidase\u8ca8\u865fP1473-5"},"content":{"rendered":"

\u751f\u7269\u7d20\u5316\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 | Biotinylated Horseradish Peroxidase\u8ca8\u865f<\/strong>P1473-5<\/a><\/span><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"\"\u751f\u7269\u7d20\u5316\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176-Biotinylated-Horseradish-Peroxidase\u8ca8\u865fP1473-5\"<\/a><\/p>\n

 <\/p>\n

\"\u00e5\u008e\u009f\u00e7\u0090\u0086\u00e4\u00bb\u008b\u00e7\u00b4\u00b9\" \u751f\u7269\u7d20(Biotin)\u662f\u4e00\u7a2e\u5c0f\u751f\u7269\u5206\u5b50(small biomolecule)\uff0c\u662f\u6240\u6709\u751f\u7269\u9ad4\u751f\u547d\u6240\u9700\u7684\u4ee3\u8b1d\u9176(metabolic enzymes)\u7684\u5fc5\u9700\u8f14\u52a9\u56e0\u5b50(essential cofactor)\u3002\u751f\u7269\u7d20(Biotin)\u662f\u4e00\u7a2e\u76f8\u5c0d\u8f03\u5c0f\u7684\u6c34\u6eb6\u6027\u5206\u5b50\uff0c\u4e0d\u6703\u5e72\u64fe\u5b83\u6240\u52a0\u5165\u7684\u5927\u5206\u5b50\u3002\u5c0d\u65bc\u751f\u7269\u6280\u8853\u61c9\u7528\u6700\u91cd\u8981\u7684\u662f\uff0c\u751f\u7269\u7d20(Biotin)\u53ef\u4ee5\u8207\u86cb\u767d\u8cea\u7d50\u5408\uff0c\u7528\u65bc\u751f\u5316\u5206\u6790\u3002\u751f\u7269\u7d20(Biotin)\u4ee5\u6975\u9ad8\u7684\u89aa\u548c\u529b\u8207\u89aa\u548c\u7d20\u548c\u93c8\u9ef4(streptavidin)\u89aa\u548c\u7d20\u7d50\u5408\uff1b\u4e00\u7a2e\u5438\u5f15\u529b\uff0c\u662f\u81ea\u7136\u754c\u4e2d\u767c\u73fe\u7684\u6700\u5f37\u548c\u6700\u7a69\u5b9a\u7684\u975e\u5171\u50f9\u76f8\u4e92\u4f5c\u7528\u4e4b\u4e00\u3002\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 (HRP) \u662f\u4e00\u7a2e\u9176\uff0c\u5b9a\u7fa9\u70ba\u6c27\u5316\u9084\u539f\u9176(oxidoreductase)\uff0c\u5b83\u5229\u7528\u6709\u6a5f\u904e\u6c27\u5316\u7269\u5316\u5408\u7269\uff0c\u4f7f\u7528\u6c2b\u904e\u6c27\u5316\u7269(hydroperoxide)\u4f5c\u70ba\u96fb\u5b50\u53d7\u9ad4(electron acceptor)\u3002\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\u984f\u8272\u9bae\u8c54\uff0c\u5c24\u5176\u662f\u5728\u8fd1\u7d2b\u5916\u5149\u4e0b\u3002\u9019\u7a2e\u7279\u6027\u4f7f\u5176\u53ef\u7528\u65bc\u9644\u8457\u5728\u201c\u900f\u660e\u201d\u86cb\u767d\u8cea\u4e0a\uff0c\u4ee5\u4fbf\u5728\u4e0d\u540c\u6ce2\u9577\u4e0b\u53ef\u4ee5\u770b\u5230\u5b83\u5011\u3002\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176(Horseradish peroxidase)\u662f\u4e00\u7a2e\u9ad8\u6d3b\u6027\u4e14\u7a69\u5b9a\u7684\u9176\uff0c\u901a\u5e38\u7528\u4f5c\u514d\u75ab\u5206\u6790\u4e2d\u7684\u6aa2\u6e2c\u8a66\u5291\uff0c\u5982\u86cb\u767d\u8cea\u5370\u8de1(western blot)\u3001\u514d\u75ab\u7d44\u7e54\u5316\u5b78(immunohistochemistry)\u548c ELISA\u3002 BioVision \u7684\u751f\u7269\u7d20\u5316\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176\u6bcf\u500b HRP \u542b\u6709 1-2 \u7a2e\u751f\u7269\u7d20\uff0c\u4f7f\u7528 BioVision \u7684\u751f\u7269\u7d20\u5b9a\u91cf\u8a66\u5291\u76d2\uff08\u6bd4\u8272\u6cd5\uff09\uff08Cat. No. K811\uff09\u901a\u904e HABA \u5206\u6790\u4f30\u8a08\uff0c\u5b83\u4e0d\u542b\u4efb\u4f55\u672a\u7d50\u5408\u7684\u751f\u7269\u7d20\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nP1473-5<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n5 mg<\/td>\n<\/tr>\n
Alternate Name<\/strong><\/td>\nBiotin-HRP, Biotin Peroxidase<\/td>\n<\/tr>\n
Appearance<\/strong><\/td>\nLyophilized powder<\/td>\n<\/tr>\n
Physical Form Description<\/strong><\/td>\nLyophilized in Phosphate Buffered Saline, pH 7.4<\/td>\n<\/tr>\n
Reconstitution Instructions<\/strong><\/td>\nReconstitute in distilled water at the concentration of 1.0 mg\/ml (or any buffer of choice)<\/td>\n<\/tr>\n
Handling<\/strong><\/td>\nCentrifuge the vial prior to opening.<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n4.0 \u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/span><\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n

 <\/p>\n

 <\/p>\n

\u00a0<\/strong><\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

\u751f\u7269\u7d20\u5316\u8fa3\u6839\u904e\u6c27\u5316\u7269\u9176 | Biotinylated Horseradish Peroxidase\u8ca8\u865fP147 […]<\/p>\n","protected":false},"author":3,"featured_media":459,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1060],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28756"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28756"}],"version-history":[{"count":2,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28756\/revisions"}],"predecessor-version":[{"id":28758,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28756\/revisions\/28758"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/459"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28756"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28756"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28756"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}