{"id":28752,"date":"2021-08-24T18:17:11","date_gmt":"2021-08-24T10:17:11","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28752"},"modified":"2022-07-19T13:56:08","modified_gmt":"2022-07-19T05:56:08","slug":"jnk-%e6%b4%bb%e6%80%a7%e7%af%a9%e9%81%b8%e8%a9%a6%e5%8a%91%e7%9b%92-jnk-activity-screening-kit%e8%b2%a8%e8%99%9fk430","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28752","title":{"rendered":"JNK \u6d3b\u6027\u7be9\u9078\u8a66\u5291\u76d2| JNK Activity screening kit\u8ca8\u865fK430"},"content":{"rendered":"

JNK \u6d3b\u6027\u7be9\u9078\u8a66\u5291\u76d2| \u00a0JNK Activity screening kit\u8ca8\u865f<\/strong>K430<\/a><\/span><\/p>\n

\"biovision\u00e5\u0095\u0086\u00e5\u0093\u0081\u00e6\u008f\u0092\u00e5\u009c\u0096\"<\/p>\n

JNK Activity screening kit<\/p>\n

\"JNK-\u6d3b\u6027\u7be9\u9078\u8a66\u5291\u76d2-JNK-Activity-screening-kit\u8ca8\u865fK430.pdf\"<\/a><\/p>\n

\u7522\u54c1\u4ecb\u7d39: c-Jun N \u7aef\u6fc0\u9176 (c-Jun N-terminal kinase , JNK) \u662f\u5728\u54fa\u4e73\u52d5\u7269\u4e2d\u9451\u5b9a\u7684\u5e7e\u500b\u4e3b\u8981 MAP \u6fc0\u9176\u7d44(MAP kinase groups)\u4e4b\u4e00\u3002\u6700\u8fd1\u7684\u8b49\u64da\u8868\u660e JNK \u7684\u6fc0\u6d3b\u53ef\u80fd\u5728\u795e\u7d93\u5143\u51cb\u4ea1(neuronal apoptosis)\u548c\u5176\u4ed6\u751f\u7406(physiological)\u548c\u75c5\u7406\u904e\u7a0b(pathological processes)\u4e2d\u8d77\u91cd\u8981\u4f5c\u7528\u3002 JNK \u6d3b\u6027\u7be9\u9078\u8a66\u5291\u76d2(JNK Activity screening kit, K430)\u5229\u7528\u8207\u7a40\u80f1\u7518\u80bd\u74ca\u8102\u7cd6\u73e0(glutathion sepharose beads)\u7d50\u5408\u7684 N \u7aef c-Jun (1-79) \u878d\u5408\u86cb\u767d\u9078\u64c7\u6027\u5730\u5f9e\u7d30\u80de\u88c2\u89e3\u7269\u4e2d\u201c\u62c9\u4e0b\u201dJNK\u3002\u6d17\u6ecc\u53bb\u9664\u975e\u7279\u7570\u6027\u7d50\u5408\u7684\u86cb\u767d\u8cea\u5f8c\uff0c\u7136\u5f8c\u5728\u51b7 ATP \u5b58\u5728\u4e0b\u9032\u884c\u6fc0\u9176\u53cd\u61c9\u3002\u7136\u5f8c\u4f7f\u7528\u78f7\u9178-c-Jun (c-Jun phosphorylation)\u7279\u7570\u6027\u6297\u9ad4\u901a\u904e\u86cb\u767d\u8cea\u5370\u8de1(Western blot)\u5206\u6790\u6e2c\u91cf c-Jun \u78f7\u9178\u5316\u3002<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n
Cat # +Size<\/strong><\/td>\nK430-40<\/td>\n<\/tr>\n
Size<\/strong><\/td>\n40 assays<\/td>\n<\/tr>\n
Detection Method<\/strong><\/td>\nWestern blot analysis with anti-Phospho-cJun (Ser 73) Specific Antibody at 1:1000 dilutions<\/td>\n<\/tr>\n
Species Reactivity<\/strong><\/td>\nMammalian<\/td>\n<\/tr>\n
Applications<\/strong><\/td>\nThe kit can be used to screen JNK activity relatively quickly for large numbers of samples.<\/td>\n<\/tr>\n
Features & Benefits<\/strong><\/td>\n\u2022 Simple procedure
\n\u2022 The kit is suitable for both research and drug discovery
\n\u2022 The assay utilizes a N-terminal c-Jun fusion protein bead to selectively \u201cpull down\u201d JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.<\/td>\n<\/tr>\n
Kit Components<\/strong><\/td>\n\u2022 Kinase Extraction Buffer
\n\u2022 c-Jun (1-79) Fusion Protein Beads
\n\u2022 Kinase Assay Buffer
\n\u2022 ATP (10 mM)
\n\u2022 Phospho-cJun Specific Antibody<\/td>\n<\/tr>\n
Storage Conditions<\/strong><\/td>\n-20\u00b0C<\/td>\n<\/tr>\n
Shipping Conditions<\/strong><\/td>\nGel Pack<\/td>\n<\/tr>\n
USAGE<\/strong><\/td>\nFor Research Use Only! Not For Use in Humans.<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\"\u00e7\u009b\u00b8\u00e9\u0097\u009c\u00e8\u00a9\u00a6\u00e5\u008a\u0091\"\u7a40\u80f1\u7518\u80bd\u904e\u6c27\u5316\u7269\u9176\u6d3b\u6027\u6e2c\u5b9a<\/span><\/a> | \u00a0FRAP\u6aa2\u6e2c<\/a> | \u7e3d\u81a0\u539f\u86cb\u767d\u5206\u6790<\/u><\/strong><\/a> | Dopamine\u591a\u5df4\u80fa\u6aa2\u6e2c<\/a> | \u4e09\u9178\u7518\u6cb9\u916f\u5206\u6790<\/a> | Cholesterol Efflux\u81bd\u56fa\u9187\u6392\u51fa\u87a2\u5149\u5206\u6790<\/a> | \u8102\u8cea\u904e\u6c27\u5316\uff08MDA<\/strong>\uff09\u6e2c\u5b9a<\/strong><\/a> | <\/strong>\u652f\u93c8\u6c28\u57fa\u9178<\/strong><\/a> BCAA<\/u><\/strong>\u6aa2\u6e2c<\/u><\/strong><\/span><\/p>\n

\"\u00e5\u00bc\u0095\u00e7\u0094\u00a8\u00e6\u0096\u0087\u00e7\u008d\u00bb\"Yu, W. et al. Polycystin-1 Protein Level Determines Activity of the G 12\/JNK Apoptosis Pathway. J. Biol. Chem. 2010 285: 10243-10251.<\/p>\n

Yu XX et al (2008) Am J Physiol Endocrinol Metab 295: E436 – E446.<\/p>\n

Shintani, Y. et al. (2006) Cancer Res. 66: 11745-11753.<\/p>\n

\u00a0<\/strong><\/p>\n\n\n\n\n\n\n\n\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n
\u8ca8\u865f9210<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g, 50 g<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9211-5<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u5206\u5b50\u7b49\u7d1aRNase\/DNase free)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6 99% (Native PAGE and SDS PAGE assay)<\/td>\n<\/tr>\n
\u8ca8\u865f9247<\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (NGS\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg, 1 g, 10 g<\/td>\n\n

\u91cd\u7d44\u9176\u5728Pichia pastoris\u4e2d\u8868\u9054\uff0c<\/p>\n

\u4e26\u9032\u884c\u5ee3\u6cdb\u7684\u7d14\u5316\u4ee5\u7372\u5f97\u6700\u9ad8\u7684<\/p>\n

\u512a\u8cea\u7684\u7522\u54c1\u3002\u984d\u5916\u7684\u7d14\u5316\u6b65\u9a5f\u5c0e\u81f4<\/p>\n

\u6eb6\u89e3\u5ea6\u986f\u8457\u589e\u52a0\uff082.5\u500d\uff09\uff0c\u589e\u52a0<\/p>\n

\u6bd4\u6d3b\u6027\u548c\u6e1b\u5c11\u7684DNA\u542b\u91cf<\/p>\n

 <\/td>\n<\/tr>\n

\u8ca8\u865f9250<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n5 ml, 25 ml, 100 m (20 mg\/ml Solution)<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n
\u8ca8\u865f9251<\/a><\/td>\n\u91cd\u7d44\u86cb\u767d\u9176K (\u6a19\u6e96\u7b49\u7d1a)<\/a><\/td>\n100 mg, 500 mg ,1 g ,10 g<\/td>\n\u7d14\u5ea6>90%<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

JNK \u6d3b\u6027\u7be9\u9078\u8a66\u5291\u76d2| \u00a0JNK Activity screening kit\u8ca8\u865fK430 JNK Acti […]<\/p>\n","protected":false},"author":3,"featured_media":28510,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[3],"tags":[1061],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28752"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28752"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28752\/revisions"}],"predecessor-version":[{"id":28755,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28752\/revisions\/28755"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28510"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28752"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28752"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28752"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}