{"id":28265,"date":"2021-08-06T16:25:42","date_gmt":"2021-08-06T08:25:42","guid":{"rendered":"http:\/\/biopioneer.com.tw\/?p=28265"},"modified":"2023-04-25T16:24:36","modified_gmt":"2023-04-25T08:24:36","slug":"bca%e8%9b%8b%e7%99%bd%e5%ae%9a%e9%87%8f%e5%88%86%e6%9e%90%e5%a5%97%e7%b5%84-visualprotein%e8%b2%a8%e8%99%9fbc03-500","status":"publish","type":"post","link":"http:\/\/biopioneer.com.tw\/?p=28265","title":{"rendered":"BCA\u86cb\u767d\u5b9a\u91cf\u5206\u6790\u5957\u7d44 | VisualProtein\u8ca8\u865fBC03-500"},"content":{"rendered":"

\u6e2c\u91cf\u86cb\u767d\u8cea\u6fc3\u5ea6(protein concentration)\u65b9\u6cd5-BCA \u86cb\u767d\u5b9a\u91cf<\/span><\/span><\/strong><\/span><\/p>\n

BCA\u86cb\u767d\u5b9a\u91cf\u5206\u6790\u5957\u7d44 | VisualProtein\u8ca8\u865fBC03-500\u00a0<\/a>\u00a0<\/span><\/strong><\/span><\/p>\n

\"\"<\/p>\n

BCA\u6700\u5e38\u7528\u7684\u6e2c\u91cf\u86cb\u767d\u8cea\u6fc3\u5ea6(protein concentration)\u7684\u65b9\u6cd5-Dual-Range BCA Protein Assay Kit<\/span><\/p>\n

Dual-Range\u2122 BCA \u86cb\u767d\u8cea\u6aa2\u6e2c\u8a66\u5291\u76d2(Dual-Range\u2122 BCA Protein Assay Kit, BC03-500)\u662f\u6700\u5e38\u7528\u7684\u86cb\u767d\u8cea\u6aa2\u6e2c\u65b9\u6cd5\u3002\u8207 Lowry \u65b9\u6cd5\u4e00\u6a23\uff0c\u8a72\u6e2c\u5b9a\u4f9d\u8cf4\u65bc\u86cb\u767d\u8cea\u5c0d Cu2+ \u96e2\u5b50\u7684\u9084\u539f\u3002\u901a\u904e\u8207\u4e8c\u8f9b\u53ef\u5be7\u9178(bicinchoninate)\u53cd\u61c9\u8f49\u5316\u70ba\u7d2b\u8272\u7269\u8cea\u4f86\u6aa2\u6e2c\u7531\u6b64\u5f62\u6210\u7684 Cu+\u3002 Dual-Range\u2122 BCA Protein Assay Kit \u8207\u8a31\u591a\u53bb\u6c61\u5291\u517c\u5bb9\uff0c\u4f46\u8207\u9084\u539f\u5291(reducing agents)\u5982 DTT\u3001DTE \u548c 2-\u5df0\u57fa\u4e59\u9187(2-Mercaptoethanol)\u7b49\u4e0d\u517c\u5bb9\u3002\u4e8c\u8f9b\u53ef\u5be7\u9178 (bicinchoninic acid , BCA)\uff0c\u8a66\u5291 A \u542b\u6709 BCA\uff0c\u8a66\u5291 B \u5247\u542b\u6709\u786b\u9178\u9285 (CuSO4)\u3002 \u4f7f\u7528\u524d\u6df7\u548c\u5169\u7a2e\u8a66\u5291\uff0c\u5728\u9e7c\u6027\u74b0\u5883\u4e0b\uff0c\u86cb\u767d\u8cea\u53ef\u5c07\u4e8c\u50f9\u9285\u96e2 \u5b50 Cu2+ \u9084\u539f\u70ba\u5169\u500b\u55ae\u50f9\u9285\u96e2\u5b50 Cu+\uff0c\u800c\u4e00\u500b Cu+ \u53ef\u87af\u5408\u5169 \u500b BCA \u5206\u5b50\uff0c\u4f7f\u8a66\u5291\u984f\u8272\u7531\u860b\u679c\u7da0\u8f49\u8b8a\u70ba\u7d2b\u8272\uff0c\u6b64\u5c08\u4e00\u6027\u5448 \u8272\u8207\u86cb\u767d\u8cea\u6fc3\u5ea6\u6210\u6b63\u6bd4\uff0c\u900f\u904e\u6aa2\u6e2c 562nm \u7684\u5438\u5149\u503c\uff0c\u4e26\u6bd4\u5c0d \u6a19\u6e96\u86cb\u767d ( \u4f8b\u5982\uff1aBSA) \u7684\u6821\u6b63\u66f2\u7dda\uff0c\u53ef\u53d6\u5f97\u86cb\u767d\u8cea\u6fc3\u5ea6\u5b9a\u91cf \u6578\u64da\u3002\u00a0\u7528\u65bc 5\u20132,000 \u03bcg\/mL \u6fc3\u5ea6\u7bc4\u570d\u5167\u7684\u7e3d\u86cb\u767d\u8cea\u7684\u6bd4\u8272\u6aa2\u6e2c\u548c\u5b9a\u91cf\u3002\u6a19\u6e96\u65b9\u6848\u70ba 20-2000 \u03bcg\/mL\uff0c\u589e\u5f37\u65b9\u6848\u70ba 5-250 \u03bcg\/mL<\/p>\n

\"\"<\/p>\n

\u25cf\u00a0\u5bec\u7dda\u6027\u7bc4\u570d\uff1a\u6a19\u6e96\u65b9\u6848\u70ba 20-2000 \u03bcg\/mL\uff0c\u589e\u5f37\u65b9\u6848\u70ba 5-250 \u03bcg\/mL<\/p>\n

\u25cf BCA \u5c0d\u4ecb\u9762\u6d3b\u6027\u5291 (detergent) \u5177\u6709\u9ad8\u8010\u53d7\u6027\uff0c\u4e0d\u6613\u53d7\u5176\u5f71\u97ff<\/p>\n

\u25cf \u6e2c\u5b9a\u5feb\u901f\uff0c\u53ef\u65bc 30 \u5206\u9418\u5b8c\u6210\u6e2c\u5b9a\uff0c\u6bd4 Lowry \u6cd5\u5feb 4 \u500d<\/p>\n

\u25cf \u5ba4\u6eab\u4fdd\u5b58\uff0c\u6548\u671f 24 \u500b\u6708<\/p>\n

\u25cf \u53f0\u7063\u751f\u7522\uff0c\u54c1\u8cea\u4fdd\u8b49<\/p>\n

\"\"<\/p>\n

\u5716 1.<\/strong>\u725b\u8840\u6e05\u767d\u86cb\u767d (BSA) \u548c\u725b\u4e19\u7a2e\u7403\u86cb\u767d (BGG) \u7684\u6a19\u6e96\u66f2\u7dda\uff0c\u63a1\u7528\u5fae\u5b54\u677f\u7a0b\u5e8f\uff0837\u00b0C\uff0c30 \u5206\u9418\uff09\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u5716 2.<\/strong>\u4f7f\u7528\u5fae\u5b54\u677f\u7a0b\u5e8f\uff0837\u00b0C\uff0c30 \u5206\u9418\uff09\uff0c\u542b 5% Triton X-100 \u6216 8% SDS \u7684 BSA \u6a19\u6e96\u66f2\u7dda\u3002<\/span><\/p>\n

\"\"<\/p>\n

\u5716 3.<\/strong>\u4f7f\u7528\u5fae\u5b54\u677f\u7a0b\u5e8f\u548c\u589e\u5f37\u65b9\u6848\uff0860\u00b0C 30 \u5206\u9418\uff09\u7684\u5fae\u5b54\u677f\u7a0b\u5e8f\u7684 BSA \u6a19\u6e96\u66f2\u7dda\u3002<\/span><\/p>\n

\"\"<\/p>\n

BC03-500 \u00a0 \u00a0Dual-Range BCA Protein Assay Kit<\/strong><\/u><\/p>\n

> Reagent A\u00a0\u00a0500 mL<\/p>\n

> Reagent B\u00a0 12 mL<\/p>\n

BSA Standard (2 mg\/mL)\u00a01 mL x 10<\/p>\n

\"\"<\/p><\/blockquote>\n

 <\/p>\n

Reference:<\/strong><\/p>\n

1. Young, Guang-Huar, et al. “The functional role of hemojuvelin in acute ischemic stroke.”\u00a0Journal of Cerebral Blood Flow & Metabolism<\/em>\u00a0(2019): 0271678X19861448.<\/p>\n

2. Chen, Sheng-Yi, et al. “Antioxidant and anti-inflammatory activities and bioactive compounds of the leaves of\u00a0Trichodesma khasianum\u00a0<\/em>clarke.”\u00a0Industrial Crops and Products<\/em>\u00a0151 (2020): 112447.<\/p>\n

3. Wang, Guan-Yu, et al. “Protective effect of rosmarinic acid-rich\u00a0Trichodesma khasianum<\/em>\u00a0Clarke leaves against ethanol-induced gastric mucosal injury in vitro and in vivo.”\u00a0Phytomedicine<\/em>\u00a0(2020): 153382.<\/p>\n

4. Hsu, Jen\u2010Ying, et al. “Aqueous extract from Pepino (Solanum muricatum Ait.<\/em>) leaves ameliorated insulin resistance, hyperlipidemia, and hyperglycemia in mice with metabolic syndrome.”\u00a0Journal of Food Biochemistry<\/em>\u00a044.12 (2020): e13518.<\/p>\n

5. Yang, Kai-Chiang, et al. “Effect of thermal treatments on the structural change and the hemostatic property of hair extracted proteins.”\u00a0Colloids and Surfaces B: Biointerfaces<\/em>\u00a0190 (2020): 110951.<\/p>\n

6. Naito, Yoshisuke, et al. “Deterioration of Myofibrils Affected by Experimental Partial Immobilization in a Septic Rat Model.”\u00a0Journal of St. Marianna University<\/em>\u00a011.2 (2020): 61-72.<\/p>\n

7. Young, Guang\u2010Huar, et al. “Modulation of adenine phosphoribosyltransferase\u2010mediated salvage pathway to accelerate diabetic wound healing.”\u00a0The FASEB Journal<\/em>\u00a035.3 (2021): e21296.<\/p>\n

8. Leyton, Esteban, et al. “DEF8 and Autophagy-Associated Genes are Altered in Mild Cognitive Impairment, Probable Alzheimer\u2019s Disease Patients and a Transgenic Model of the Disease.”\u00a0Journal of Alzheimer’s Disease\u00a0Preprint<\/em>\u00a0(2021): 1-16.<\/p>\n

9. Hsu, Jen-Ying, et al. “Aqueous Extract of Pepino Leaves Ameliorates Palmitic Acid-Induced Hepatocellular Lipotoxicity via Inhibition of Endoplasmic Reticulum Stress and Apoptosis.”\u00a0Antioxidants<\/em>\u00a010.6 (2021): 903.<\/p>\n

10. Chen, Hong, et al. “Promoting effects of MiR-135b on human multiple myeloma cells via regulation of the Wnt\/\u03b2-catenin\/Versican signaling pathway.”\u00a0Cytokine<\/em>\u00a0142 (2021): 155495.<\/p>\n

11. Chen, Ying-Yin, et al. “4\u2011Acetylantroquinonol B enhances cell death and inhibits autophagy by downregulating the PI3K\/Akt\/MDR1 pathway in gemcitabine\u2011resistant pancreatic cancer cells.”\u00a0Oncology Letters<\/em>\u00a023.4 (2022): 1-13.<\/p>\n

\"\u64cd\u4f5c\u65b9\u6cd5\"<\/a><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
BR01-500<\/strong><\/td>\nBradford \u86cb\u767d\u8cea\u5b9a\u91cf\u8a66\u5291<\/td>\n\u00a0Dual-Range Bradford Reagent (1X), 500 ml ,<\/td>\n<\/td>\n<\/tr>\n
BR05-500<\/strong><\/td>\n5X Bradford \u86cb\u767d\u8cea\u5b9a\u91cf\u8a66\u5291<\/td>\n\u00a0Dual-Range Bradford Reagent (5X), 500ml<\/td>\n<\/td>\n<\/tr>\n
BR05-500-K<\/strong><\/td>\n5X Bradford \u86cb\u767d\u8cea\u5b9a\u91cf\u8a66\u5291\u7d44<\/td>\n\n

\u00a0Dual-Range Bradford Protein Assay Kit, (500 ml +10* 2mg\/ml<\/p>\n

BSA standard)<\/td>\n

<\/td>\n<\/tr>\n
BP01-1L<\/strong><\/td>\n\u897f\u65b9\u58a8\u9ede\u6cd5\u906e\u84cb\u6db2<\/td>\n\u00a0BlockPRO Blocking Buffer, 500mlx2<\/td>\n<\/td>\n<\/tr>\n
BF01-1L<\/strong><\/td>\n\u897f\u65b9\u58a8\u9ede\u6cd5\u906e\u84cb\u6db2-Protein Free<\/td>\n\u00a0BlockPRO Protein-Free Blocking Buffer, 500mlx2<\/td>\n<\/td>\n<\/tr>\n
BF20-50P<\/strong><\/td>\n\u897f\u65b9\u58a8\u9ede\u6cd5\u906e\u84cb\u6db2-Protein Free<\/td>\n\n

\u00a0BlockPRO Protein-Free Blocking Buffer, 20X, 20\u5305\uff0c\u6bcf\u53052.5 mL\uff0c<\/p>\n

\u4f7f\u7528\u524d\u52a0\u5165500mL ddH2O \u56de\u6eb6\u6210 1X<\/td>\n

<\/td>\n<\/tr>\n
\u3000<\/strong><\/td>\n<\/td>\n\u00a0\u897f\u65b9\u58a8\u9ede\u6cd5\u906e\u84cb\u6db2-Protein Free, 20X<\/td>\n<\/td>\n<\/tr>\n
BM01-500<\/strong><\/td>\n\u897f\u65b9\u58a8\u9ede\u6cd5\u906e\u84cb\u6db2-1\u5206\u9418\u914d\u65b9<\/td>\n\u00a0BlockPRO 1 Min Protein-Free Blocking Buffer, 500ml<\/td>\n<\/td>\n<\/tr>\n
SP01-500<\/strong><\/td>\n\u6297\u9ad4\u53bb\u9664\u8a66\u5291<\/td>\n\u00a0StripPRO 1 Min Stripping Buffer, 500ml<\/td>\n<\/td>\n<\/tr>\n
LF01-500<\/strong><\/td>\n\u51b7\u5149\u8a66\u5291\u7d44- Prime<\/td>\n\u00a0LumiFlash Prime Chemiluminescent Substrate,( 250mL solnA + 250mL soln B)<\/td>\n<\/td>\n<\/tr>\n
LF08-500<\/strong><\/td>\n\u51b7\u5149\u8a66\u5291\u7d44- Ultima<\/td>\n\u00a0LumiFlash Ultima Chemiluminescent Substrate, (250mL solnA + 250mL soln B)<\/td>\n<\/td>\n<\/tr>\n
LF16-500<\/strong><\/td>\n\u51b7\u5149\u8a66\u5291\u7d44- Infinity<\/td>\n\u00a0LumiFlash Infinity Chemiluminescent Substrate, (250mL solnA + 250mL soln B)<\/td>\n<\/td>\n<\/tr>\n
VC01-250<\/strong><\/td>\n\u9810\u67d3\u86cb\u767d\u8cea\u6a19\u6e96\u6db2<\/td>\n\u00a0VisColorTM<\/sup>\u00a0Pre-Stained Protein Marker, 250ul x 1<\/td>\n<\/td>\n<\/tr>\n
VC01-500<\/strong><\/td>\n\u9810\u67d3\u86cb\u767d\u8cea\u6a19\u6e96\u6db2<\/td>\n\u00a0VisColorTM<\/sup>\u00a0Pre-Stained Protein Marker, 250ul x 2<\/td>\n<\/td>\n<\/tr>\n
VC03-250<\/strong><\/td>\n\u5168\u7bc4\u570d\u9810\u67d3\u86cb\u767d\u8cea\u6a19\u6e96\u6db2<\/td>\n\n

\u00a0VisColorTM<\/sup>\u00a0Full-Range Pre-Stained Protein Marker, 250ul x 1<\/p>\n

 <\/td>\n

<\/td>\n<\/tr>\n
VC03-500<\/strong><\/td>\n\u5168\u7bc4\u570d\u9810\u67d3\u86cb\u767d\u8cea\u6a19\u6e96\u6db2<\/td>\n\n

\u00a0VisColorTM<\/sup>\u00a0Full-Range Pre-Stained Protein Marker, 250ul x 2<\/p>\n

 <\/td>\n

<\/td>\n<\/tr>\n
LH03-10<\/strong><\/td>\n\u51b7\u5149\u7b46<\/td>\n\n

\u00a0LuminolPen HRP system,<\/p>\n

(\u7d04 150 \u5f35 Western blot’s membranes \u00a0\u4f7f\u7528)<\/td>\n

<\/td>\n<\/tr>\n
LH03-50<\/strong><\/td>\n\u51b7\u5149\u7b46<\/td>\n\n

\u00a0LuminolPen HRP system,<\/p>\n

(\u7d041000 \u5f35 Western blot’s membranes \u00a0\u4f7f\u7528)<\/td>\n

<\/td>\n<\/tr>\n
LH05-10<\/strong><\/td>\n\u51b7\u5149\u7b46 EZ\u7248<\/td>\n\u00a0LuminolPen EZ HRP System, (\u7d04 150 \u5f35 Western blot’s membranes \u4f7f\u7528)<\/td>\n<\/td>\n<\/tr>\n
LH05-50<\/strong><\/td>\n\u51b7\u5149\u7b46 EZ\u7248<\/td>\n\u00a0LuminolPen EZ HRP System, (\u7d04 1000 \u5f35 Western blot’s membranes \u4f7f\u7528)<\/td>\n<\/td>\n<\/tr>\n
RB10-1L<\/strong><\/td>\nSDS-PAGE \u96fb\u6cf3\u7de9\u885d\u6db2<\/td>\n\u00a0SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), 500 ml *2<\/td>\n<\/td>\n<\/tr>\n
RB500P<\/strong><\/td>\n\u00a0\u00a0SDS-PAGE \u96fb\u6cf3\u7de9\u885d\u6db2\u6fc3\u7e2e\u5305<\/td>\n\n

\u00a0SDS-PAGE Running Buffer , 10X (Tris-Glycine, pH 8.3), (20\u5305\uff0c\u6bcf<\/p>\n

\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165500mL ddH2O \u56de\u6eb6\u6210 1X)<\/td>\n

<\/td>\n<\/tr>\n
WTB10-1L<\/strong><\/td>\nWestern Blot Transfer Buffer<\/td>\n\u00a0Western Blot Transfer Buffer, 10X (Tris-Glycine, pH 8.3), 500 ml *2\u00a0 \u897f\u65b9\u58a8\u9ede\u6cd5\u8f49\u5370\u7de9\u885d\u6db2<\/td>\n<\/td>\n<\/tr>\n
WTB500P<\/strong><\/td>\n\u897f\u65b9\u58a8\u9ede\u6cd5\u8f49\u5370\u7de9\u885d\u6db2\u6fc3\u7e2e\u5305<\/td>\n\n

\u00a0Western Blot Transfer Buffer,(20\u5305\uff0c\u6bcf\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165500mL<\/p>\n

ddH2O \u56de\u6eb6\u6210 1X)<\/td>\n

<\/td>\n<\/tr>\n
TBS10-1L<\/strong><\/td>\n\u00a0TBS\u7de9\u885d\u6db2<\/td>\n\u00a0TBS Buffer, 10X, 500 mL*2<\/td>\n<\/td>\n<\/tr>\n
TBS200P<\/strong><\/td>\nTBS \u7de9\u885d\u6db2\u6fc3\u7e2e\u5305<\/td>\n\n

\u00a0TBS Buffer, 25X, (30\u5305\uff0c\u6bcf\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165200 mL ddH2O \u56de\u6eb6\u6210<\/p>\n

1X)<\/td>\n

<\/td>\n<\/tr>\n
TBST10-1L<\/strong><\/td>\nTBST \u7de9\u885d\u6db2<\/td>\n\u00a0TBS Tween-20 Buffer, 10X, 500 mL*2<\/td>\n<\/td>\n<\/tr>\n
TBST200P<\/strong><\/td>\nTBST \u7de9\u885d\u6db2\u6fc3\u7e2e\u5305<\/td>\n\n

\u00a0TBS Tween-20 Buffer, 25X, (30\u5305\uff0c\u6bcf\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165200 mL<\/p>\n

ddH2O \u56de\u6eb6\u6210 1X)<\/td>\n

<\/td>\n<\/tr>\n
PBS10-1L<\/strong><\/td>\n\u00a0PBS \u7de9\u885d\u6db2<\/td>\n\u00a0PBS Buffer, 10X , 500 mL*2<\/td>\n<\/td>\n<\/tr>\n
PBS200P<\/strong><\/td>\nPBS \u7de9\u885d\u6db2<\/td>\n\u00a0PBS Buffer, 25X, (30\u5305\uff0c\u6bcf\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165200 mL ddH2O \u56de\u6eb6\u6210 1X)<\/td>\n<\/td>\n<\/tr>\n
PBST10-1L<\/strong><\/td>\nPBST \u7de9\u885d\u6db2<\/td>\n\u00a0PBS Tween-20 Buffer, 10X, 500 mL*2<\/td>\n<\/td>\n<\/tr>\n
PBST200P<\/strong><\/td>\nPBST \u7de9\u885d\u6db2<\/td>\n\n

\u00a0PBS Tween-20 Buffer, 25X, (30\u5305\uff0c\u6bcf\u5305\u9808\u65bc\u4f7f\u7528\u524d\u52a0\u5165200 mL<\/p>\n

ddH2O \u56de\u6eb6\u6210 1X)<\/td>\n

<\/td>\n<\/tr>\n
SBN06-15<\/strong><\/td>\n6x Laemmli SDS Sample Buffer, non-reducing<\/td>\n\u00a06x Laemmli SDS Sample Buffer, non-reducing, 15ml<\/td>\n<\/td>\n<\/tr>\n
SBR06-15<\/strong><\/td>\n\u00a06x Laemmli SDS Sample Buffer, reducing<\/td>\n\u00a06x Laemmli SDS Sample Buffer, reducing, 15ml<\/td>\n<\/td>\n<\/tr>\n
VP01-125<\/strong><\/td>\n\u5168\u86cb\u767d\u8cea\u8ca0\u67d3\u8272\u8a66\u5291\u7d44<\/td>\n\n

\u00a0VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL<\/p>\n

soln2)<\/td>\n

<\/td>\n<\/tr>\n
VP01-500<\/strong><\/td>\n\u5168\u86cb\u767d\u8cea\u8ca0\u67d3\u8272\u8a66\u5291\u7d44<\/td>\n\n

\u00a0VisPRO 5 Minutes Protein Stain Kit, (500mL soln 1 + 500mL soln<\/p>\n

2)<\/td>\n

<\/td>\n<\/tr>\n
VP05-125<\/strong><\/td>\n\u5168\u86cb\u767d\u8cea\u8ca0\u67d3\u8272\u8a66\u5291\u7d44- \u4e94\u500d\u6fc3\u7e2e\u6db2<\/td>\n\n

\u00a05x VisPRO 5 Minutes Protein Stain Kit, (125mL soln 1 + 125mL soln 2)<\/p>\n

 <\/td>\n

<\/td>\n<\/tr>\n
VP05-500<\/strong><\/td>\n\u5168\u86cb\u767d\u8cea\u8ca0\u67d3\u8272\u8a66\u5291\u7d44- \u4e94\u500d\u6fc3\u7e2e\u6db2<\/td>\n\u00a05x VisPRO 5 Minutes Protein Stain Kit, (250mL soln 1 + 250mL soln 2)<\/td>\n<\/td>\n<\/tr>\n
\n

PP03-2C<\/strong><\/p>\n

\u3000<\/strong><\/td>\n

\u78f7\u9178\u5316\u86cb\u8cea\u8403\u53d6\u8a66\u5291\u7d44<\/td>\n\u00a0PhosPRO Phosphoprotein Enrichment Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock)<\/td>\n<\/td>\n<\/tr>\n
PP03-6C<\/strong><\/td>\n\u78f7\u9178\u5316\u86cb\u8cea\u8403\u53d6\u8a66\u5291\u7d44<\/td>\n\n

\u00a0PhosPRO Phosphoprotein Enrichment Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) +<\/p>\n

(100mL Elution buffer+100 mL Stringent buffer stock )<\/td>\n

<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
\n

PP03-5E\u00a0<\/strong><\/p>\n

\u3000<\/strong><\/td>\n

\u78f7\u9178\u5316\u86cb\u8cea\u8403\u53d6\u8a66\u5291\u7d44<\/td>\n\n

\u00a0PhosPRO Phosphoprotein Enrichment Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) +<\/p>\n

(100mL Elution buffer+100 mL Stringent buffer stock)<\/td>\n

<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
\n

PP05-2C<\/strong><\/p>\n

\u3000<\/strong><\/td>\n

\u00a0PhosPRO Phosphoprotein Purification Kit, (2 pre-packed column + 2mL Lysis buffer+250mL System buffer) +(100mL Elution buffer+100 mL Stringent buffer stock)<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
\n

PP05-6C<\/strong><\/p>\n

\u3000<\/strong><\/td>\n

\u78f7\u9178\u5316\u86cb\u767d\u8cea\u7d14\u5316\u8a66\u5291\u7d44<\/td>\nPhosPRO Phosphoprotein Purification Kit, (6 pre-packed column + 2mL Lysis buffer+250mL System buffer) +\u00a0(100mL Elution buffer+100 mL Stringent buffer stock)\u00a0 \u78f7\u9178\u5316\u86cb\u767d\u8cea\u7d14\u5316\u8a66\u5291\u7d44<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
\n

PP05-5E<\/strong><\/p>\n

\u3000<\/strong><\/td>\n

\u78f7\u9178\u5316\u86cb\u767d\u8cea\u7d14\u5316\u8a66\u5291\u7d44<\/td>\n\u00a0PhosPRO Phosphoprotein Purification Kit, (3 mL Resin + 2mL Lysis buffer+250mL System buffer) +\u00a0(100mL Elution buffer+100 mL Stringent buffer stock )<\/td>\n<\/td>\n<\/tr>\n
<\/td>\n<\/tr>\n
EP05-30<\/strong><\/td>\n\u5168\u86cb\u767d\u8cea\u8403\u53d6\u8a66\u5291<\/td>\n\u00a0ExtractPRO Protein Extraction Reagent<\/td>\n<\/td>\n<\/tr>\n
FP01-5<\/strong><\/td>\n\u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2\u8a66\u5291\u7d44<\/td>\n\u00a0FracPRO Protein Fractionation Kit, (10mL Soln1+ 10mL Soln2 +7.5mL Soln3+1.5mL Soln4)<\/td>\n<\/td>\n<\/tr>\n
FP01-50<\/strong><\/td>\n\u80de\u5668\u86cb\u767d\u8cea\u5206\u96e2\u8a66\u5291\u7d44<\/td>\n\u00a0FracPRO Protein Fractionation Kit, (100mL Soln1 + 100mL Soln2+ 75mL Soln3 +15mL Soln4)<\/td>\n<\/td>\n<\/tr>\n
HB01-1L<\/strong><\/td>\n\u4fc3\u878d\u5408\u7624\u7d30\u80de\u751f\u9577\u56e0\u5b50<\/td>\n\u00a0HybriMore, Hybridoma cloning factor, for 1L medium dilution<\/td>\n<\/td>\n<\/tr>\n
IF01-4N<\/strong><\/td>\n\u5feb\u901f\u514d\u75ab\u4f50\u5291<\/td>\n\u00a0ImmunoFast Adjuvant, for 4 injections to mouse<\/td>\n<\/td>\n<\/tr>\n
DG03-1P<\/strong><\/td>\nDIGE\u86cb\u767d\u8cea\u6a19\u8a18\u8a66\u5291\u7d44<\/td>\n\u00a0DIGE-Kit, (Cy2 400pmole + Cy3 400pmole + Cy5 400pmole+ 20ml DMF)<\/td>\n<\/td>\n<\/tr>\n
GLP01<\/strong><\/td>\n\u8ca0\u67d3\u81a0\u7247\u5149\u7bb1<\/td>\n\u00a0Gel Lighting Plate 21 x 29.7 cm(A4)<\/td>\n<\/td>\n<\/tr>\n
BLP01<\/strong><\/td>\n\u6b63\u67d3\u81a0\u7247\u5149\u7bb1<\/td>\n\u00a0Band Lighting Plate 21 x 29.7 cm(A4)<\/td>\n<\/td>\n<\/tr>\n
BSB01<\/strong><\/td>\n\u9ed1\u67d3\u76d2<\/td>\n\u00a0Black Staining Box 13 x 13 x 4 cm (For mini gel)<\/td>\n<\/td>\n<\/tr>\n
BSB02<\/strong><\/td>\n\u9ed1\u67d3\u76d2<\/td>\n\u00a0Black Staining Box 17.5 x 17.5 x 4.5 cm (For regular gel)<\/td>\n<\/td>\n<\/tr>\n
BSB03<\/strong><\/td>\n\u9ed1\u67d3\u76d2<\/td>\n\u00a0Black Staining Box 20 x 24 x 5 cm<\/td>\n<\/td>\n<\/tr>\n
BSB04<\/strong><\/td>\n\u9ed1\u67d3\u76d2<\/td>\n\u00a0Black Staining Box 27 x 27 x 5 cm<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u6e2c\u91cf\u86cb\u767d\u8cea\u6fc3\u5ea6(protein concentration)\u65b9\u6cd5-BCA \u86cb\u767d\u5b9a\u91cf BCA\u86cb\u767d\u5b9a\u91cf\u5206\u6790\u5957\u7d44 | […]<\/p>\n","protected":false},"author":3,"featured_media":28266,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5626,3],"tags":[1085],"_links":{"self":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28265"}],"collection":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=28265"}],"version-history":[{"count":3,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28265\/revisions"}],"predecessor-version":[{"id":41691,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/posts\/28265\/revisions\/41691"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=\/wp\/v2\/media\/28266"}],"wp:attachment":[{"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=28265"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=28265"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/biopioneer.com.tw\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=28265"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}